(Circular) (Six-base) MAPSORT of: pFN21AB0499.seq Check: 1609 from: 1 to: 6967 pFN21AB0499 6967 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2512 4777 278 Size: 53 83 186 936 976 2265 2468 Fragments arranged by size: 2468 2265 976 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 4310 1937 Size: 1452 921 4594 Fragments arranged by size: 4594 1452 921 Acc65I G'GTAC_C Cuts at: 2228 4288 2228 Size: 2060 4907 AclI AA'CG_TT Cuts at: 1563 5094 5467 1563 Size: 3531 373 3063 Fragments arranged by size: 3531 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2943 3917 5030 6414 6682 1632 Size: 1311 974 1113 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1113 974 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6770 AflIII A'CryG_T Cuts at: 1203 2807 5866 6843 1203 Size: 1604 3059 977 1327 Fragments arranged by size: 3059 1604 1327 977 AgeI A'CCGG_T Cuts at: 5798 5798 Size: 6967 AhdI GACnn_n'nnGTC Cuts at: 5696 5696 Size: 6967 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6967 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6967 AlwNI CAG_nnn'CTG Cuts at: 4710 6282 4710 Size: 1572 5395 ApaI G_GGCC'C Cuts at: 2033 2033 Size: 6967 ApaLI G'TGCA_C Cuts at: 2493 5024 6180 2493 Size: 2531 1156 3280 Fragments arranged by size: 3280 2531 1156 ApoI r'AATT_y Cuts at: 1493 2318 2540 3179 3604 3977 4054 4064 4276 Size: 825 222 639 425 373 77 10 212 Cuts at: 4276 4502 4654 6763 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 825 639 425 373 226 222 212 152 77 10 AseI AT'TA_AT Cuts at: 160 1032 5521 160 Size: 872 4489 1606 Fragments arranged by size: 4489 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 AvaI C'yCGr_G Cuts at: 1870 1943 4292 1870 Size: 73 2349 4545 Fragments arranged by size: 4545 2349 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2220 2253 2220 Size: 33 6934 BamHI G'GATC_C Cuts at: 4297 4664 4297 Size: 367 6600 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 4288 5743 618 Size: 325 217 627 441 2060 1455 1842 Fragments arranged by size: 2060 1842 1455 627 441 325 217 BanII G_rGCy'C Cuts at: 1391 2033 4286 1391 Size: 642 2253 4072 Fragments arranged by size: 4072 2253 642 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3423 3894 928 Size: 971 1524 471 4001 Fragments arranged by size: 4001 1524 971 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5158 5192 1903 Size: 34 3221 34 3678 Fragments arranged by size: 3678 3221 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4859 6069 4859 Size: 1210 5757 BclI T'GATC_A Cuts at: 1548 2116 1548 Size: 568 6399 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5578 136 Size: 107 122 71 631 605 3906 1525 Fragments arranged by size: 3906 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4123 6962 4123 Size: 2839 4128 BlpI GC'TnA_GC Cuts at: 4384 4384 Size: 6967 Bme1580I G_kGCm'C Cuts at: 1790 2033 2497 5028 6184 1790 Size: 243 464 2531 1156 2573 Fragments arranged by size: 2573 2531 1156 464 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6967 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2985 3068 4698 Size: 416 53 83 399 24 1568 83 1630 Cuts at: 4698 5656 442 Size: 958 1753 Fragments arranged by size: 1753 1630 1568 958 416 399 83 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6967 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3000 4107 5627 1239 Size: 1761 1107 1520 2579 Fragments arranged by size: 2579 1761 1520 1107 Bpu10I CC'TnA_GC Cuts at: 4172 4172 Size: 6967 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3834 3866 5092 5957 6255 3834 Size: 32 1226 865 298 4546 Fragments arranged by size: 4546 1226 865 298 32 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5630 882 Size: 617 4131 2219 Fragments arranged by size: 4131 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3094 4758 493 Size: 711 1890 1664 2702 Fragments arranged by size: 2702 1890 1664 711 BsaBI GATnn'nnATC Cuts at: 3278 6961 3278 Size: 3683 3284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2509 4774 5156 5791 Size: 53 83 186 936 976 2265 382 635 Cuts at: 5791 275 Size: 1451 Fragments arranged by size: 2265 1451 976 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5400 5798 6072 6219 6630 5400 Size: 398 274 147 411 5737 Fragments arranged by size: 5737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2998 3028 1178 Size: 30 165 30 1595 30 5117 Fragments arranged by size: 5117 1595 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2055 1186 Size: 869 6098 BseYI C'CCAG_C Cuts at: 6170 6666 6170 Size: 496 6471 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6394 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4417 5178 6206 1540 Size: 275 186 2416 761 1028 2301 Fragments arranged by size: 2416 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2497 5028 5113 6184 2497 Size: 2531 85 1071 3280 Fragments arranged by size: 3280 2531 1071 85 BsmI GAATG_Cn' Cuts at: 2447 2701 3192 4478 4571 2447 Size: 254 491 1286 93 4843 Fragments arranged by size: 4843 1286 491 254 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2497 4286 5028 5113 6184 1391 Size: 399 243 464 1789 742 85 1071 2174 Fragments arranged by size: 2174 1789 1071 742 464 399 243 85 BspEI T'CCGG_A Cuts at: 6630 6630 Size: 6967 BspHI T'CATG_A Cuts at: 2952 4856 6586 2952 Size: 1904 1730 3333 Fragments arranged by size: 3333 1904 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3496 4322 844 Size: 2652 826 3489 Fragments arranged by size: 3489 2652 826 BsrBI CCG'CTC Cuts at: 1394 1823 4854 1394 Size: 429 3031 3507 Fragments arranged by size: 3507 3031 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5462 5636 65 Size: 1183 4214 174 1396 Fragments arranged by size: 4214 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5611 5798 1700 Size: 110 3801 187 2869 Fragments arranged by size: 3801 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6967 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6967 BssSI C'ACGA_G Cuts at: 5027 6039 5027 Size: 1012 5955 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6967 BstBI TT'CG_AA Cuts at: 5771 5771 Size: 6967 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6967 BstYI r'GATC_y Cuts at: 1972 4123 4297 4664 5049 5066 6518 6604 6616 Size: 2151 174 367 385 17 1452 86 12 Cuts at: 6616 6962 1972 Size: 346 1977 Fragments arranged by size: 2151 1977 1452 385 367 346 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2979 2979 Size: 6967 BtgI C'CryG_G Cuts at: 513 1065 2025 513 Size: 552 960 5455 Fragments arranged by size: 5455 960 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6228 Fragments arranged by size: 6228 683 56 BtsI GCAGTG_nn' Cuts at: 4483 5277 5297 4483 Size: 794 20 6153 Fragments arranged by size: 6153 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6967 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6932 DraI TTT'AAA Cuts at: 2388 3588 4254 4271 2388 Size: 1200 666 17 5084 Fragments arranged by size: 5084 1200 666 17 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5974 809 Size: 507 4658 1802 Fragments arranged by size: 4658 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3672 4414 5303 Size: 54 1425 50 275 18 1842 742 889 Cuts at: 5303 6932 8 Size: 1629 43 Fragments arranged by size: 1842 1629 1425 889 742 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4414 1537 Size: 275 2602 4090 Fragments arranged by size: 4090 2602 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2359 2760 4158 4206 4258 4897 1353 Size: 1006 401 1398 48 52 639 3423 Fragments arranged by size: 3423 1398 1006 639 401 52 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5554 5928 6074 387 Size: 1064 138 3965 374 146 1280 Fragments arranged by size: 3965 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2943 3000 3917 4107 5030 5627 6414 Size: 393 1311 57 917 190 923 597 787 Cuts at: 6414 6682 1239 Size: 268 1524 Fragments arranged by size: 1524 1311 923 917 787 597 393 268 190 57 EcoNI CCTnn'n_nnAGG Cuts at: 4089 4089 Size: 6967 EcoRI G'AATT_C Cuts at: 2540 4276 4654 2540 Size: 1736 378 4853 Fragments arranged by size: 4853 1736 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4235 4235 Size: 6967 FspI TGC'GCA Cuts at: 5473 5473 Size: 6967 HaeII r_GCGC'y Cuts at: 1678 3072 6114 1678 Size: 1394 3042 2531 Fragments arranged by size: 3042 2531 1394 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2597 2629 2998 3030 3092 Size: 32 135 32 1025 32 369 32 62 Cuts at: 3092 3124 3539 3571 3575 3607 5608 5640 5682 Size: 32 415 32 4 32 2001 32 42 Cuts at: 5682 5714 1373 Size: 32 2626 Fragments arranged by size: 2626 2001 1025 415 369 135 62 42 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2148 4046 4311 4557 669 Size: 988 281 210 1898 265 246 3079 Fragments arranged by size: 3079 1898 988 281 265 246 210 HindIII A'AGCT_T Cuts at: 748 748 Size: 6967 HpaI GTT'AAC Cuts at: 2148 4557 2148 Size: 2409 4558 KpnI G_GTAC'C Cuts at: 2232 4292 2232 Size: 2060 4907 MfeI C'AATT_G Cuts at: 2428 2514 4566 2428 Size: 86 2052 4829 Fragments arranged by size: 4829 2052 86 MluI A'CGCG_T Cuts at: 6843 6843 Size: 6967 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2062 6081 6265 1305 Size: 757 4019 184 2007 Fragments arranged by size: 4019 2007 757 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3674 10 Size: 54 1425 343 1842 3303 Fragments arranged by size: 3303 1842 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4925 5284 5443 518 Size: 648 3759 359 159 2042 Fragments arranged by size: 3759 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 4383 5060 6208 6453 1606 Size: 421 2356 677 1148 245 2120 Fragments arranged by size: 2356 2120 1148 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6415 NdeI CA'TA_TG Cuts at: 387 387 Size: 6967 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6967 NotI GC'GGCC_GC Cuts at: 4414 4414 Size: 6967 NspI r_CATG'y Cuts at: 1132 1229 2352 4205 4325 5870 1132 Size: 97 1123 1853 120 1545 2229 Fragments arranged by size: 2229 1853 1545 1123 120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6967 PciI A'CATG_T Cuts at: 5866 5866 Size: 6967 PflMI CCAn_nnn'nTGG Cuts at: 1108 2618 1108 Size: 1510 5457 PfoI T'CCnGG_A Cuts at: 2800 3016 4084 2800 Size: 216 1068 5683 Fragments arranged by size: 5683 1068 216 PmeI GTTT'AAAC Cuts at: 4271 4271 Size: 6967 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 6549 6581 5036 Size: 32 1481 32 5422 Fragments arranged by size: 5422 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6967 PsiI TTA'TAA Cuts at: 4537 4537 Size: 6967 PspOMI G'GGCC_C Cuts at: 2029 2029 Size: 6967 PstI C_TGCA'G Cuts at: 830 3631 4319 830 Size: 2801 688 3478 Fragments arranged by size: 3478 2801 688 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6967 SacII CC_GC'GG Cuts at: 2028 2028 Size: 6967 SalI G'TCGA_C Cuts at: 1936 4309 1936 Size: 2373 4594 SapI GCTCTTCn'nnn_ Cuts at: 2359 2359 Size: 6967 SbfI CC_TGCA'GG Cuts at: 4319 4319 Size: 6967 ScaI AGT'ACT Cuts at: 5215 6927 5215 Size: 1712 5255 SfcI C'TryA_G Cuts at: 826 1045 3627 4315 5450 6131 6322 826 Size: 219 2582 688 1135 681 191 1471 Fragments arranged by size: 2582 1471 1135 688 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6967 SmaI CCC'GGG Cuts at: 1872 4294 1872 Size: 2422 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3849 3881 5071 5972 6234 820 Size: 197 926 1906 32 1190 901 262 1553 Fragments arranged by size: 1906 1553 1190 926 901 262 197 32 SnaBI TAC'GTA Cuts at: 493 3094 4758 493 Size: 2601 1664 2702 Fragments arranged by size: 2702 2601 1664 SpeI A'CTAG_T Cuts at: 152 152 Size: 6967 SphI G_CATG'C Cuts at: 1132 2352 4325 1132 Size: 1220 1973 3774 Fragments arranged by size: 3774 1973 1220 SspI AAT'ATT Cuts at: 5 52 2531 4891 5 Size: 47 2479 2360 2081 Fragments arranged by size: 2479 2360 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6967 StyI C'CwwG_G Cuts at: 513 1065 3782 4406 513 Size: 552 2717 624 3074 Fragments arranged by size: 3074 2717 624 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3220 4994 5011 5164 5349 5794 325 Size: 1255 1640 1774 17 153 185 445 1498 Fragments arranged by size: 1774 1640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2993 5213 6925 Size: 274 80 33 51 1443 1016 2220 1712 Cuts at: 6925 96 Size: 138 Fragments arranged by size: 2220 1712 1443 1016 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2080 2112 2928 2960 3859 3891 6927 6959 2080 Size: 32 816 32 899 32 3036 32 2088 Fragments arranged by size: 3036 2088 899 816 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6967 XbaI T'CTAG_A Cuts at: 4303 4303 Size: 6967 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6967 XmaI C'CCGG_G Cuts at: 1870 4292 1870 Size: 2422 4545 XmnI GAAnn'nnTTC Cuts at: 3190 5096 3190 Size: 1906 5061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2510 4775 276 Size: 53 83 186 936 976 2265 2468 Fragments arranged by size: 2468 2265 976 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI