Restriction map for pFN21AB0499
 (Linear) (Six-base) MAPSORT of: KIBB0499.seq  Check: 6057  from: 1  to: 2262



KIBB0499.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AatII G_ACGT'C

Cuts at:      0     507    2262 
   Size:        507    1755
 
AccI GT'mk_AC

Cuts at:      0    1384    2262 
   Size:       1384     878
 
Acc65I G'GTAC_C

Cuts at:      0     223    2262 
   Size:        223    2039
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     938    1912    2262 
   Size:        938     974     350

  Fragments arranged by size: 

                 974     938     350
 
AflIII A'CryG_T

Cuts at:      0     802    2262 
   Size:        802    1460
 
ApaI G_GGCC'C

Cuts at:      0      28    2262 
   Size:         28    2234
 
ApaLI G'TGCA_C

Cuts at:      0     488    2262 
   Size:        488    1774
 
ApoI r'AATT_y

Cuts at:      0     313     535    1174    1599    1972    2049    2059    2262 
   Size:        313     222     639     425     373      77      10     203

  Fragments arranged by size: 

                 639     425     373     313     222     203      77      10
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     215     248    2262 
   Size:        215      33    2014

  Fragments arranged by size: 

                2014     215      33
 
BanI G'GyrC_C

Cuts at:      0     223    2262 
   Size:        223    2039
 
BanII G_rGCy'C

Cuts at:      0      28    2262 
   Size:         28    2234
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1418    1889    2262 
   Size:       1418     471     373

  Fragments arranged by size: 

                1418     471     373
 
BclI T'GATC_A

Cuts at:      0     111    2262 
   Size:        111    2151
 
BglII A'GATC_T

Cuts at:      0    2118    2262 
   Size:       2118     144
 
Bme1580I G_kGCm'C

Cuts at:      0      28     492    2262 
   Size:         28     464    1770

  Fragments arranged by size: 

                1770     464      28
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     980    1063    2262 
   Size:        980      83    1199

  Fragments arranged by size: 

                1199     980      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     995    2102    2262 
   Size:        995    1107     160

  Fragments arranged by size: 

                1107     995     160
 
Bpu10I CC'TnA_GC

Cuts at:      0    2167    2262 
   Size:       2167      95
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1829    1861    2262 
   Size:       1829      32     401

  Fragments arranged by size: 

                1829     401      32
 
BsaAI yAC'GTr

Cuts at:      0    1089    2262 
   Size:       1089    1173
 
BsaBI GATnn'nnATC

Cuts at:      0    1273    2262 
   Size:       1273     989
 
BsaHI Gr'CG_yC

Cuts at:      0     504    2262 
   Size:        504    1758
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     993    1023    2262 
   Size:        993      30    1239

  Fragments arranged by size: 

                1239     993      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      50    2262 
   Size:         50    2212
 
BsiHKAI G_wGCw'C

Cuts at:      0     492    2262 
   Size:        492    1770
 
BsmI GAATG_Cn'

Cuts at:      0     442     696    1187    2262 
   Size:        442     254     491    1075

  Fragments arranged by size: 

                1075     491     442     254
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     492    2262 
   Size:         28     464    1770

  Fragments arranged by size: 

                1770     464      28
 
BspHI T'CATG_A

Cuts at:      0     947    2262 
   Size:        947    1315
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1491    2262 
   Size:       1491     771
 
BstYI r'GATC_y

Cuts at:      0    2118    2262 
   Size:       2118     144
 
Bsu36I CC'TnA_GG

Cuts at:      0     974    2262 
   Size:        974    1288
 
BtgI C'CryG_G

Cuts at:      0      20    2262 
   Size:         20    2242
 
DraI TTT'AAA

Cuts at:      0     383    1583    2249    2262 
   Size:        383    1200     666      13

  Fragments arranged by size: 

                1200     666     383      13
 
EaeI y'GGCC_r

Cuts at:      0    1667    2262 
   Size:       1667     595
 
EarI CTCTTCn'nnn_

Cuts at:      0     354     755    2153    2201    2262 
   Size:        354     401    1398      48      61

  Fragments arranged by size: 

                1398     401     354      61      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     938     995    1912    2102    2262 
   Size:        938      57     917     190     160

  Fragments arranged by size: 

                 938     917     190     160      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2084    2262 
   Size:       2084     178
 
EcoRI G'AATT_C

Cuts at:      0     535    2262 
   Size:        535    1727
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2230    2262 
   Size:       2230      32
 
HaeII r_GCGC'y

Cuts at:      0    1067    2262 
   Size:       1067    1195
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     592     624     993    1025    1087    1119    1534    1566 
   Size:        592      32     369      32      62      32     415      32

Cuts at:   1566    1570    1602    2262 
   Size:          4      32     660

  Fragments arranged by size: 

                 660     592     415     369      62      32      32      32
                  32      32       4
 
HincII GTy'rAC

Cuts at:      0     143    2041    2262 
   Size:        143    1898     221

  Fragments arranged by size: 

                1898     221     143
 
HpaI GTT'AAC

Cuts at:      0     143    2262 
   Size:        143    2119
 
KpnI G_GTAC'C

Cuts at:      0     227    2262 
   Size:        227    2035
 
MfeI C'AATT_G

Cuts at:      0     423     509    2262 
   Size:        423      86    1753

  Fragments arranged by size: 

                1753     423      86
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      57    2262 
   Size:         57    2205
 
MscI TGG'CCA

Cuts at:      0    1669    2262 
   Size:       1669     593
 
MspA1I CmG'CkG

Cuts at:      0      22    2262 
   Size:         22    2240
 
NspI r_CATG'y

Cuts at:      0     347    2200    2262 
   Size:        347    1853      62

  Fragments arranged by size: 

                1853     347      62
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     613    2262 
   Size:        613    1649
 
PfoI T'CCnGG_A

Cuts at:      0     795    1011    2079    2262 
   Size:        795     216    1068     183

  Fragments arranged by size: 

                1068     795     216     183
 
PspOMI G'GGCC_C

Cuts at:      0      24    2262 
   Size:         24    2238
 
PstI C_TGCA'G

Cuts at:      0    1626    2262 
   Size:       1626     636
 
SacII CC_GC'GG

Cuts at:      0      23    2262 
   Size:         23    2239
 
SapI GCTCTTCn'nnn_

Cuts at:      0     354    2262 
   Size:        354    1908
 
SfcI C'TryA_G

Cuts at:      0    1622    2262 
   Size:       1622     640
 
SmlI C'TyrA_G

Cuts at:      0    1844    1876    2262 
   Size:       1844      32     386

  Fragments arranged by size: 

                1844     386      32
 
SnaBI TAC'GTA

Cuts at:      0    1089    2262 
   Size:       1089    1173
 
SphI G_CATG'C

Cuts at:      0     347    2262 
   Size:        347    1915
 
SspI AAT'ATT

Cuts at:      0     526    2262 
   Size:        526    1736
 
StyI C'CwwG_G

Cuts at:      0    1777    2262 
   Size:       1777     485
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1215    2262 
   Size:       1215    1047
 
TatI w'GTAC_w

Cuts at:      0     988    2262 
   Size:        988    1274
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      75     107     923     955    1854    1886    2262 
   Size:         75      32     816      32     899      32     376

  Fragments arranged by size: 

                 899     816     376      75      32      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1185    2262 
   Size:       1185    1077
 
ZraI GAC'GTC

Cuts at:      0     505    2262 
   Size:        505    1757
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     ApaI    ApaLI     ApoI
     BaeI     BanI    BanII     BbsI     BclI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BstYI   Bsu36I     BtgI
     DraI     EaeI     EarI  Eco57MI    EcoNI    EcoRI     FalI    HaeII
    Hin4I   HincII     HpaI     KpnI     MfeI     MmeI     MscI   MspA1I
     NspI    PflMI     PfoI   PspOMI     PstI    SacII     SapI     SfcI
     SmlI    SnaBI     SphI     SspI     StyI    TaqII     TatI     TstI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI     BsaI    BsaWI    BseYI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRV
     FseI     FspI    FspAI  HindIII     KasI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI
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