Restriction map for
pFN21AA1007
(Circular) (Six-base) MAPSORT of: pFN21AA1007.seq Check: 4246 from: 1 to: 11818
pFN21AA1007 11818 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2499 4093 9628 278
Size: 53 83 186 936 963 1594 5535 2468
Fragments arranged by size:
5535 2468 1594 963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 9161 1937
Size: 1243 5981 4594
Fragments arranged by size:
5981 4594 1243
Acc65I G'GTAC_C
Cuts at: 8814 9139 8814
Size: 325 11493
AclI AA'CG_TT
Cuts at: 1563 9945 10318 1563
Size: 8382 373 3063
Fragments arranged by size:
8382 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3231 3406 5477 5585 5936 7676 7898 8824
Size: 1599 175 2071 108 351 1740 222 926
Cuts at: 8824 9881 11265 11533 1632
Size: 1057 1384 268 1917
Fragments arranged by size:
2071 1917 1740 1599 1384 1057 926 351
268 222 175 108
AflII C'TTAA_G
Cuts at: 820 1017 2624 6751 820
Size: 197 1607 4127 5887
Fragments arranged by size:
5887 4127 1607 197
AflIII A'CryG_T
Cuts at: 1203 8448 10717 11694 1203
Size: 7245 2269 977 1327
Fragments arranged by size:
7245 2269 1327 977
AgeI A'CCGG_T
Cuts at: 10649 10649
Size: 11818
AhdI GACnn_n'nnGTC
Cuts at: 10547 10547
Size: 11818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3993 5955 7767 9082 1184
Size: 2809 1962 1812 1315 3920
Fragments arranged by size:
3920 2809 1962 1812 1315
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 11786
AlwNI CAG_nnn'CTG
Cuts at: 2224 5389 6199 6539 6609 7592 8191 9561 11133
Size: 3165 810 340 70 983 599 1370 1572
Cuts at: 11133 2224
Size: 2909
Fragments arranged by size:
3165 2909 1572 1370 983 810 599 340
70
ApaLI G'TGCA_C
Cuts at: 9875 11031 9875
Size: 1156 10662
ApoI r'AATT_y
Cuts at: 1493 2260 2407 3061 3105 3816 4219 5363 5468
Size: 767 147 654 44 711 403 1144 105
Cuts at: 5468 5920 6566 7940 7965 8377 8746 9011 9127
Size: 452 646 1374 25 412 369 265 116
Cuts at: 9127 9353 9505 11614 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1374 1144 767 711 654 646
452 412 403 369 265 226 152 147
116 105 44 25
AseI AT'TA_AT
Cuts at: 160 1032 5199 7371 7869 8022 10372 160
Size: 872 4167 2172 498 153 2350 1606
Fragments arranged by size:
4167 2350 2172 1606 872 498 153
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11818
AvaI C'yCGr_G
Cuts at: 1870 1943 6945 9143 1870
Size: 73 5002 2198 4545
Fragments arranged by size:
5002 4545 2198 73
BamHI G'GATC_C
Cuts at: 2718 4829 9148 9515 2718
Size: 2111 4319 367 5021
Fragments arranged by size:
5021 4319 2111 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4433 6100 7125 8814 9110
Size: 325 217 627 2646 1667 1025 1689 296
Cuts at: 9110 9139 10594 618
Size: 29 1455 1842
Fragments arranged by size:
2646 1842 1689 1667 1455 1025 627 325
296 217 29
BanII G_rGCy'C
Cuts at: 1391 2765 5262 9137 1391
Size: 1374 2497 3875 4072
Fragments arranged by size:
4072 3875 2497 1374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6521 7664 8945 928
Size: 971 4622 1143 1281 3801
Fragments arranged by size:
4622 3801 1281 1143 971
BbvCI CC'TCA_GC
Cuts at: 3377 6412 3377
Size: 3035 8783
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2815 2849 6695 6729 7764 7798 10009
Size: 34 878 34 3846 34 1035 34 2211
Cuts at: 10009 10043 1903
Size: 34 3678
Fragments arranged by size:
3846 3678 2211 1035 878 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4713 8375 8605 9710 10920 4713
Size: 3662 230 1105 1210 5611
Fragments arranged by size:
5611 3662 1210 1105 230
BclI T'GATC_A
Cuts at: 1548 3661 5592 1548
Size: 2113 1931 7774
Fragments arranged by size:
7774 2113 1931
BfrBI ATG'CAT
Cuts at: 3575 3622 7486 3575
Size: 47 3864 7907
Fragments arranged by size:
7907 3864 47
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10429 136
Size: 107 122 71 631 605 8757 1525
Fragments arranged by size:
8757 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2422 2467 3255 7336 11813 2422
Size: 45 788 4081 4477 2427
Fragments arranged by size:
4477 4081 2427 788 45
BlpI GC'TnA_GC
Cuts at: 5790 7734 9235 5790
Size: 1944 1501 8373
Fragments arranged by size:
8373 1944 1501
Bme1580I G_kGCm'C
Cuts at: 1790 4436 5700 8273 9028 9879 11035 1790
Size: 2646 1264 2573 755 851 1156 2573
Fragments arranged by size:
2646 2573 2573 1264 1156 851 755
BmgBI CAC'GTC
Cuts at: 1318 3776 1318
Size: 2458 9360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2437 3170 4439
Size: 416 53 83 399 24 1020 733 1269
Cuts at: 4439 4693 4891 9549 10507 442
Size: 254 198 4658 958 1753
Fragments arranged by size:
4658 1753 1269 1020 958 733 416 399
254 198 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4107 1056
Size: 3051 8767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4008 5953 7335 4008
Size: 1945 1382 8491
Fragments arranged by size:
8491 1945 1382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2986 3921 4092 5184 6866 7380 7509 8517
Size: 1747 935 171 1092 1682 514 129 1008
Cuts at: 8517 8609 10478 1239
Size: 92 1869 2579
Fragments arranged by size:
2579 1869 1747 1682 1092 1008 935 514
171 129 92
Bpu10I CC'TnA_GC
Cuts at: 3377 4664 5043 5055 6412 6477 6897 7728 8001
Size: 1287 379 12 1357 65 420 831 273
Cuts at: 8001 3377
Size: 7194
Fragments arranged by size:
7194 1357 1287 831 420 379 273 65
12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6876 7485 8086 9943 10808 11106 6876
Size: 609 601 1857 865 298 7588
Fragments arranged by size:
7588 1857 865 609 601 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5486 7101 10481 882
Size: 617 3987 1615 3380 2219
Fragments arranged by size:
3987 3380 2219 1615 617
BsaAI yAC'GTr
Cuts at: 493 1204 9609 493
Size: 711 8405 2702
Fragments arranged by size:
8405 2702 711
BsaBI GATnn'nnATC
Cuts at: 2208 3327 5907 8535 11812 2208
Size: 1119 2580 2628 3277 2214
Fragments arranged by size:
3277 2628 2580 2214 1119
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2496 4090 9625 10007
Size: 53 83 186 936 963 1594 5535 382
Cuts at: 10007 10642 275
Size: 635 1451
Fragments arranged by size:
5535 1594 1451 963 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2732 8811 10251 10649 10923 11070 11481 2732
Size: 6079 1440 398 274 147 411 3069
Fragments arranged by size:
6079 3069 1440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 2538 2568 8065
Size: 30 165 30 822 30 283 30 5497
Cuts at: 8065 8095 1178
Size: 30 4901
Fragments arranged by size:
5497 4901 822 283 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 2546 2558 5070 1186
Size: 1357 3 12 2512 7934
Fragments arranged by size:
7934 2512 1357 12 3
BseYI C'CCAG_C
Cuts at: 2343 6777 7410 8986 9087 11021 11517 2343
Size: 4434 633 1576 101 1934 496 2644
Fragments arranged by size:
4434 2644 1934 1576 633 496 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2310 2858 3747 3999 4842 1163
Size: 573 574 548 889 252 843 8139
Fragments arranged by size:
8139 889 843 574 573 548 252
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9057 9268 10029 11057 1540
Size: 275 186 7056 211 761 1028 2301
Fragments arranged by size:
7056 2301 1028 761 275 211 186
BsiHKAI G_wGCw'C
Cuts at: 2361 2673 2765 2998 3928 4384 5517 7462 7729
Size: 312 92 233 930 456 1133 1945 267
Cuts at: 7729 8649 8711 9879 9964 11035 2361
Size: 920 62 1168 85 1071 3144
Fragments arranged by size:
3144 1945 1168 1133 1071 930 920 456
312 267 233 92 85 62
BsmI GAATG_Cn'
Cuts at: 2925 3144 4232 4298 5727 6303 7052 7486 8145
Size: 219 1088 66 1429 576 749 434 659
Cuts at: 8145 8797 9329 9422 2925
Size: 652 532 93 5321
Fragments arranged by size:
5321 1429 1088 749 659 652 576 532
434 219 93 66
BsmBI CGTCTCn'nnnn_
Cuts at: 3862 3862
Size: 11818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2673 2765 2998 3928 4384 4436
Size: 399 571 312 92 233 930 456 52
Cuts at: 4436 5262 5517 5700 7462 7729 8273 8649 8711
Size: 826 255 183 1762 267 544 376 62
Cuts at: 8711 9028 9137 9879 9964 11035 1391
Size: 317 109 742 85 1071 2174
Fragments arranged by size:
2174 1762 1071 930 826 742 571 544
456 399 376 317 312 267 255 233
183 109 92 85 62 52
BspEI T'CCGG_A
Cuts at: 2732 11481 2732
Size: 8749 3069
BspHI T'CATG_A
Cuts at: 6375 7362 7921 9707 11437 6375
Size: 987 559 1786 1730 6756
Fragments arranged by size:
6756 1786 1730 987 559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 9173 844
Size: 8329 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 9705 1394
Size: 429 7882 3507
Fragments arranged by size:
7882 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2297 3633 5645 6727 7396 7452 7815
Size: 1183 1049 1336 2012 1082 669 56 363
Cuts at: 7815 8466 8667 10313 10487 65
Size: 651 201 1646 174 1396
Fragments arranged by size:
2012 1646 1396 1336 1183 1082 1049 669
651 363 201 174 56
BsrFI r'CCGG_y
Cuts at: 1700 1810 10462 10649 1700
Size: 110 8652 187 2869
Fragments arranged by size:
8652 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4417 6029 9016 96
Size: 4321 1612 2987 2898
Fragments arranged by size:
4321 2987 2898 1612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11818
BssSI C'ACGA_G
Cuts at: 7826 9878 10890 7826
Size: 2052 1012 8754
Fragments arranged by size:
8754 2052 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3496 6166 794
Size: 2702 2670 6446
Fragments arranged by size:
6446 2702 2670
BstBI TT'CG_AA
Cuts at: 6907 10622 6907
Size: 3715 8103
BstEII G'GTnAC_C
Cuts at: 1184 5107 5149 1184
Size: 3923 42 7853
Fragments arranged by size:
7853 3923 42
BstXI CCAn_nnnn'nTGG
Cuts at: 5760 8946 5760
Size: 3186 8632
BstYI r'GATC_y
Cuts at: 1972 2422 2467 2718 2961 3255 4829 4987 7336
Size: 450 45 251 243 294 1574 158 2349
Cuts at: 7336 7494 9148 9515 9900 9917 11369 11455 11467
Size: 158 1654 367 385 17 1452 86 12
Cuts at: 11467 11813 1972
Size: 346 1977
Fragments arranged by size:
2349 1977 1654 1574 1452 450 385 367
346 294 251 243 158 158 86 45
17 12
Bsu36I CC'TnA_GG
Cuts at: 2127 7532 2127
Size: 5405 6413
BtgI C'CryG_G
Cuts at: 513 1065 2664 4020 6036 6762 7100 513
Size: 552 1599 1356 2016 726 338 5231
Fragments arranged by size:
5231 2016 1599 1356 726 552 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2196 487
Size: 683 56 970 10109
Fragments arranged by size:
10109 970 683 56
BtsI GCAGTG_nn'
Cuts at: 2200 2962 4293 4404 6167 7820 9334 10128 10148
Size: 762 1331 111 1763 1653 1514 794 20
Cuts at: 10148 2200
Size: 3870
Fragments arranged by size:
3870 1763 1653 1514 1331 794 762 111
20
ClaI AT'CG_AT
Cuts at: 1554 6220 1554
Size: 4666 7152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6858 6893 529
Size: 35 6294 35 5454
Fragments arranged by size:
6294 5454 35 35
DraI TTT'AAA
Cuts at: 2319 3864 4618 4873 9122 2319
Size: 1545 754 255 4249 5015
Fragments arranged by size:
5015 4249 1545 754 255
DraIII CAC_nnn'GTG
Cuts at: 3779 7125 3779
Size: 3346 8472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2495 4474 6077 10825 809
Size: 507 1179 1979 1603 4748 1802
Fragments arranged by size:
4748 1979 1802 1603 1179 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4487 5229 6879
Size: 54 1425 50 275 18 2657 742 1650
Cuts at: 6879 7081 7594 9265 10154 11783 8
Size: 202 513 1671 889 1629 43
Fragments arranged by size:
2657 1671 1650 1629 1425 889 742 513
275 202 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9265 1537
Size: 275 7453 4090
Fragments arranged by size:
7453 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 3344 3425 5331 5393 6702 6909 9748
Size: 953 1038 81 1906 62 1309 207 2839
Cuts at: 9748 1353
Size: 3423
Fragments arranged by size:
3423 2839 1906 1309 1038 953 207 81
62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3906 10405 10779 10925 387
Size: 1064 138 2317 6499 374 146 1280
Fragments arranged by size:
6499 2317 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2763 2763
Size: 11818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2986 3231 3406 3921 4092 5184 5477
Size: 393 1354 245 175 515 171 1092 293
Cuts at: 5477 5585 5936 6866 7380 7509 7676 7898 8517
Size: 108 351 930 514 129 167 222 619
Cuts at: 8517 8609 8824 9881 10478 11265 11533 1239
Size: 92 215 1057 597 787 268 1524
Fragments arranged by size:
1524 1354 1092 1057 930 787 619 597
515 514 393 351 293 268 245 222
215 175 171 167 129 108 92
EcoNI CCTnn'n_nnAGG
Cuts at: 5897 8264 5897
Size: 2367 9451
EcoO109I rG'GnC_Cy
Cuts at: 2542 6536 7885 2542
Size: 3994 1349 6475
Fragments arranged by size:
6475 3994 1349
EcoRI G'AATT_C
Cuts at: 2407 9127 9505 2407
Size: 6720 378 4720
Fragments arranged by size:
6720 4720 378
EcoRV GAT'ATC
Cuts at: 8737 8737
Size: 11818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 5099 5839 2605
Size: 2494 740 8584
Fragments arranged by size:
8584 2494 740
FspI TGC'GCA
Cuts at: 2636 8579 8673 10324 2636
Size: 5943 94 1651 4130
Fragments arranged by size:
5943 4130 1651 94
HaeII r_GCGC'y
Cuts at: 1678 8219 10965 1678
Size: 6541 2746 2531
Fragments arranged by size:
6541 2746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 3355 3387 3859
Size: 32 135 32 434 32 1317 32 472
Cuts at: 3859 3891 4126 4158 5627 5659 7360 7392 7651
Size: 32 235 32 1469 32 1701 32 259
Cuts at: 7651 7683 8104 8136 8541 8573 10459 10491 10533
Size: 32 421 32 405 32 1886 32 42
Cuts at: 10533 10565 1373
Size: 32 2626
Fragments arranged by size:
2626 1886 1701 1469 1317 472 434 421
405 259 235 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 9162 9408 669
Size: 988 281 7224 246 3079
Fragments arranged by size:
7224 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2459 3972 6792 7611 748
Size: 1711 1513 2820 819 4955
Fragments arranged by size:
4955 2820 1711 1513 819
HpaI GTT'AAC
Cuts at: 9408 9408
Size: 11818
KpnI G_GTAC'C
Cuts at: 8818 9143 8818
Size: 325 11493
MfeI C'AATT_G
Cuts at: 4030 6177 6484 8796 9417 4030
Size: 2147 307 2312 621 6431
Fragments arranged by size:
6431 2312 2147 621 307
MluI A'CGCG_T
Cuts at: 11694 11694
Size: 11818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2578 4598 6146 7458 7609 7992 10932 11116
Size: 1273 2020 1548 1312 151 383 2940 184
Cuts at: 11116 1305
Size: 2007
Fragments arranged by size:
2940 2020 2007 1548 1312 1273 383 184
151
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4489 5231 6881 10
Size: 54 1425 343 2657 742 1650 4947
Fragments arranged by size:
4947 2657 1650 1425 742 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 3007 3521 4772 5510 6858 7262
Size: 648 1542 299 514 1251 738 1348 404
Cuts at: 7262 9776 10135 10294 518
Size: 2514 359 159 2042
Fragments arranged by size:
2514 2042 1542 1348 1251 738 648 514
404 359 299 159
MspA1I CmG'CkG
Cuts at: 1606 2347 2864 4181 6338 6464 7414 7592 9234
Size: 741 517 1317 2157 126 950 178 1642
Cuts at: 9234 9911 11059 11304 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2157 2120 1642 1317 1148 950 741 677
517 245 178 126
NcoI C'CATG_G
Cuts at: 513 1065 4020 7100 513
Size: 552 2955 3080 5231
Fragments arranged by size:
5231 3080 2955 552
NdeI CA'TA_TG
Cuts at: 387 7090 387
Size: 6703 5115
NheI G'CTAG_C
Cuts at: 1052 4103 1052
Size: 3051 8767
NotI GC'GGCC_GC
Cuts at: 9265 9265
Size: 11818
NsiI A_TGCA'T
Cuts at: 3577 3624 7488 3577
Size: 47 3864 7907
Fragments arranged by size:
7907 3864 47
NspI r_CATG'y
Cuts at: 1132 1229 2168 3209 3454 3626 6360 6587 6863
Size: 97 939 1041 245 172 2734 227 276
Cuts at: 6863 8009 8452 9176 10721 1132
Size: 1146 443 724 1545 2229
Fragments arranged by size:
2734 2229 1545 1146 1041 939 724 443
276 245 227 172 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11818
PciI A'CATG_T
Cuts at: 8448 10717 8448
Size: 2269 9549
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4070 4340 6199 6211 7057 7417 8749 1108
Size: 2962 270 1859 12 846 360 1332 4177
Fragments arranged by size:
4177 2962 1859 1332 846 360 270 12
PfoI T'CCnGG_A
Cuts at: 3094 5274 6192 3094
Size: 2180 918 8720
Fragments arranged by size:
8720 2180 918
PmeI GTTT'AAAC
Cuts at: 9122 9122
Size: 11818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4366 4398 6357 6389 9887 9919 11400 11432 4366
Size: 32 1959 32 3498 32 1481 32 4752
Fragments arranged by size:
4752 3498 1959 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2542 7885 2542
Size: 5343 6475
PshAI GACnn'nnGTC
Cuts at: 1613 4978 6753 1613
Size: 3365 1775 6678
Fragments arranged by size:
6678 3365 1775
PsiI TTA'TAA
Cuts at: 3245 9388 3245
Size: 6143 5675
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4159 4191 7890 7922 4159
Size: 32 3699 32 8055
Fragments arranged by size:
8055 3699 32 32
PstI C_TGCA'G
Cuts at: 830 4399 4611 5539 6523 7077 9170 830
Size: 3569 212 928 984 554 2093 3478
Fragments arranged by size:
3569 3478 2093 984 928 554 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11818
PvuII CAG'CTG
Cuts at: 2347 2864 4181 6338 6464 7414 7592 2347
Size: 517 1317 2157 126 950 178 6573
Fragments arranged by size:
6573 2157 1317 950 517 178 126
SacI G_AGCT'C
Cuts at: 2765 2765
Size: 11818
SalI G'TCGA_C
Cuts at: 1936 9160 1936
Size: 7224 4594
SapI GCTCTTCn'nnn_
Cuts at: 3344 3425 5331 6909 3344
Size: 81 1906 1578 8253
Fragments arranged by size:
8253 1906 1578 81
SbfI CC_TGCA'GG
Cuts at: 9170 9170
Size: 11818
ScaI AGT'ACT
Cuts at: 10066 11778 10066
Size: 1712 10106
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 11818
SfcI C'TryA_G
Cuts at: 826 1045 4395 4607 5497 5535 6519 6984 7039
Size: 219 3350 212 890 38 984 465 55
Cuts at: 7039 7073 8588 9166 10301 10982 11173 826
Size: 34 1515 578 1135 681 191 1471
Fragments arranged by size:
3350 1515 1471 1135 984 890 681 578
465 219 212 191 55 38 34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11818
SmaI CCC'GGG
Cuts at: 1872 9145 1872
Size: 7273 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 6751 6891 7500 8065 9922
Size: 197 926 681 4127 140 609 565 1857
Cuts at: 9922 10823 11085 820
Size: 901 262 1553
Fragments arranged by size:
4127 1857 1553 926 901 681 609 565
262 197 140
SnaBI TAC'GTA
Cuts at: 493 9609 493
Size: 9116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11818
SphI G_CATG'C
Cuts at: 1132 3626 9176 1132
Size: 2494 5550 3774
Fragments arranged by size:
5550 3774 2494
SspI AAT'ATT
Cuts at: 5 52 8210 9742 5
Size: 47 8158 1532 2081
Fragments arranged by size:
8158 2081 1532 47
StuI AGG'CCT
Cuts at: 1517 3710 7530 8071 1517
Size: 2193 3820 541 5264
Fragments arranged by size:
5264 3820 2193 541
StyI C'CwwG_G
Cuts at: 513 1065 3197 3535 4020 5372 5759 7100 7888
Size: 552 2132 338 485 1352 387 1341 788
Cuts at: 7888 8945 9257 513
Size: 1057 312 3074
Fragments arranged by size:
3074 2132 1352 1341 1057 788 552 485
387 338 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4208 7555 7676 9043 9845 9862 10015
Size: 1255 2628 3347 121 1367 802 17 153
Cuts at: 10015 10200 10645 325
Size: 185 445 1498
Fragments arranged by size:
3347 2628 1498 1367 1255 802 445 185
153 121 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2374 4417 6029
Size: 274 80 33 51 1443 397 2043 1612
Cuts at: 6029 6057 7694 9016 10064 11776 96
Size: 28 1637 1322 1048 1712 138
Fragments arranged by size:
2043 1712 1637 1612 1443 1322 1048 397
274 138 80 51 33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2953 2985 11778 11810 2953
Size: 32 8793 32 2961
Fragments arranged by size:
8793 2961 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2275 3873 1316
Size: 959 1598 9261
Fragments arranged by size:
9261 1598 959
XbaI T'CTAG_A
Cuts at: 4982 9154 4982
Size: 4172 7646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2570 5757 2570
Size: 3187 8631
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11818
XmaI C'CCGG_G
Cuts at: 1870 9143 1870
Size: 7273 4545
XmnI GAAnn'nnTTC
Cuts at: 2905 9947 2905
Size: 7042 4776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2497 4091 9626 276
Size: 53 83 186 936 963 1594 5535 2468
Fragments arranged by size:
5535 2468 1594 963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI RsrII SacII SanDI SfiI
SfoI SrfI SwaI