(Circular) (Six-base) MAPSORT of: pFN21AE1007.seq Check: 4386 from: 1 to: 5293 pFN21AE1007 5293 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2347 2428 2347 Size: 81 5212 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2376 3103 278 Size: 53 83 186 936 840 727 2468 Fragments arranged by size: 2468 936 840 727 186 83 53 AccI GT'mk_AC Cuts at: 1937 2211 2636 1937 Size: 274 425 4594 Fragments arranged by size: 4594 425 274 Acc65I G'GTAC_C Cuts at: 2614 2614 Size: 5293 AclI AA'CG_TT Cuts at: 1563 2592 3420 3793 1563 Size: 1029 828 373 3063 Fragments arranged by size: 3063 1029 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3356 4740 5008 1632 Size: 1724 1384 268 1917 Fragments arranged by size: 1917 1724 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5096 AflIII A'CryG_T Cuts at: 1203 4192 5169 1203 Size: 2989 977 1327 Fragments arranged by size: 2989 1327 977 AgeI A'CCGG_T Cuts at: 4124 4124 Size: 5293 AhdI GACnn_n'nnGTC Cuts at: 2563 4022 2563 Size: 1459 3834 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5293 AlwNI CAG_nnn'CTG Cuts at: 2368 3036 4608 2368 Size: 668 1572 3053 Fragments arranged by size: 3053 1572 668 ApaLI G'TGCA_C Cuts at: 3350 4506 3350 Size: 1156 4137 ApoI r'AATT_y Cuts at: 1493 2602 2828 2980 5089 1493 Size: 1109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1109 226 152 AseI AT'TA_AT Cuts at: 160 1032 3847 160 Size: 872 2815 1606 Fragments arranged by size: 2815 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 AvaI C'yCGr_G Cuts at: 1870 1943 2510 2618 1870 Size: 73 567 108 4545 Fragments arranged by size: 4545 567 108 73 BamHI G'GATC_C Cuts at: 2623 2990 2623 Size: 367 4926 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2516 2614 4069 618 Size: 325 217 627 729 98 1455 1842 Fragments arranged by size: 1842 1455 729 627 325 217 98 BanII G_rGCy'C Cuts at: 1391 2408 2540 2612 1391 Size: 1017 132 72 4072 Fragments arranged by size: 4072 1017 132 72 BbeI G_GCGC'C Cuts at: 2520 2520 Size: 5293 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3484 3518 1903 Size: 34 1547 34 3678 Fragments arranged by size: 3678 1547 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3185 4395 3185 Size: 1210 4083 BclI T'GATC_A Cuts at: 1548 1548 Size: 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 3904 136 Size: 107 122 71 631 605 394 1838 1525 Fragments arranged by size: 1838 1525 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 5288 5288 Size: 5293 BlpI GC'TnA_GC Cuts at: 2498 2710 2498 Size: 212 5081 Bme1580I G_kGCm'C Cuts at: 1790 2140 3354 4510 1790 Size: 350 1214 1156 2573 Fragments arranged by size: 2573 1214 1156 350 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5293 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3024 3982 442 Size: 416 53 83 399 24 1607 958 1753 Fragments arranged by size: 1753 1607 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5293 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2536 2095 Size: 441 4852 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2501 3953 1239 Size: 830 432 1452 2579 Fragments arranged by size: 2579 1452 830 432 Bpu10I CC'TnA_GC Cuts at: 2303 2303 Size: 5293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2605 3418 4283 4581 2605 Size: 813 865 298 3317 Fragments arranged by size: 3317 865 813 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2332 3956 882 Size: 617 833 1624 2219 Fragments arranged by size: 2219 1624 833 617 BsaAI yAC'GTr Cuts at: 493 1204 3084 493 Size: 711 1880 2702 Fragments arranged by size: 2702 1880 711 BsaBI GATnn'nnATC Cuts at: 5287 5287 Size: 5293 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2201 2373 2517 3100 Size: 53 83 186 936 668 172 144 583 Cuts at: 3100 3482 4117 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 668 635 583 382 186 172 144 83 53 BsaWI w'CCGG_w Cuts at: 3726 4124 4398 4545 4956 3726 Size: 398 274 147 411 4063 Fragments arranged by size: 4063 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5068 Fragments arranged by size: 5068 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2320 2483 1186 Size: 1134 163 3996 Fragments arranged by size: 3996 1134 163 BseYI C'CCAG_C Cuts at: 4496 4992 4496 Size: 496 4797 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 2381 1163 Size: 573 516 129 4075 Fragments arranged by size: 4075 573 516 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2743 3504 4532 1540 Size: 275 186 742 761 1028 2301 Fragments arranged by size: 2301 1028 761 742 275 186 BsiHKAI G_wGCw'C Cuts at: 2309 2408 2540 3354 3439 4510 2309 Size: 99 132 814 85 1071 3092 Fragments arranged by size: 3092 1071 814 132 99 85 BsmI GAATG_Cn' Cuts at: 2804 2897 2804 Size: 93 5200 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2309 2408 2540 2612 3354 3439 Size: 399 350 169 99 132 72 742 85 Cuts at: 3439 4510 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 169 132 99 85 72 BspEI T'CCGG_A Cuts at: 4956 4956 Size: 5293 BspHI T'CATG_A Cuts at: 3182 4912 3182 Size: 1730 3563 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2325 2347 2428 2648 844 Size: 1481 22 81 220 3489 Fragments arranged by size: 3489 1481 220 81 22 BsrBI CCG'CTC Cuts at: 1394 1823 3180 1394 Size: 429 1357 3507 Fragments arranged by size: 3507 1357 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3788 3962 65 Size: 1183 2540 174 1396 Fragments arranged by size: 2540 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3937 4124 1700 Size: 110 2127 187 2869 Fragments arranged by size: 2869 2127 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5293 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5293 BssSI C'ACGA_G Cuts at: 3353 4365 3353 Size: 1012 4281 BstAPI GCAn_nnn'nTGC Cuts at: 794 2359 794 Size: 1565 3728 BstBI TT'CG_AA Cuts at: 4097 4097 Size: 5293 BstEII G'GTnAC_C Cuts at: 1184 2257 2278 1184 Size: 1073 21 4199 Fragments arranged by size: 4199 1073 21 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2290 2269 Size: 21 5272 BstYI r'GATC_y Cuts at: 1972 2623 2990 3375 3392 4844 4930 4942 5288 Size: 651 367 385 17 1452 86 12 346 Cuts at: 5288 1972 Size: 1977 Fragments arranged by size: 1977 1452 651 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2310 2526 513 Size: 552 939 306 216 3280 Fragments arranged by size: 3280 939 552 306 216 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4554 Fragments arranged by size: 4554 683 56 BtsI GCAGTG_nn' Cuts at: 2223 2809 3603 3623 2223 Size: 586 794 20 3893 Fragments arranged by size: 3893 794 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5293 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5258 DraI TTT'AAA Cuts at: 2597 2597 Size: 5293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4300 809 Size: 507 2984 1802 Fragments arranged by size: 2984 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2409 2577 Size: 54 1425 50 275 18 228 351 168 Cuts at: 2577 2740 3629 5258 8 Size: 163 889 1629 43 Fragments arranged by size: 1629 1425 889 351 275 228 168 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2740 1537 Size: 275 928 4090 Fragments arranged by size: 4090 928 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3223 1353 Size: 1870 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 2102 3880 4254 4400 387 Size: 1064 138 495 18 1778 374 146 1280 Fragments arranged by size: 1778 1280 1064 495 374 146 138 18 EcoICRI GAG'CTC Cuts at: 2406 2538 2406 Size: 132 5161 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2501 3356 3953 4740 5008 1239 Size: 393 437 432 855 597 787 268 1524 Fragments arranged by size: 1524 855 787 597 437 432 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2294 2321 2294 Size: 27 5266 EcoO109I rG'GnC_Cy Cuts at: 2110 2299 2110 Size: 189 5104 EcoRI G'AATT_C Cuts at: 2602 2980 2602 Size: 378 4915 FspI TGC'GCA Cuts at: 2546 3799 2546 Size: 1253 4040 HaeII r_GCGC'y Cuts at: 1678 2520 4440 1678 Size: 842 1920 2531 Fragments arranged by size: 2531 1920 842 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2549 2581 3934 3966 4008 Size: 32 135 32 977 32 1353 32 42 Cuts at: 4008 4040 1373 Size: 32 2626 Fragments arranged by size: 2626 1353 977 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 2883 669 Size: 988 281 699 246 3079 Fragments arranged by size: 3079 988 699 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5293 HpaI GTT'AAC Cuts at: 2883 2883 Size: 5293 KasI G'GCGC_C Cuts at: 2516 2516 Size: 5293 KpnI G_GTAC'C Cuts at: 2618 2618 Size: 5293 MfeI C'AATT_G Cuts at: 2892 2892 Size: 5293 MluI A'CGCG_T Cuts at: 5169 5169 Size: 5293 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4407 4591 1305 Size: 3102 184 2007 Fragments arranged by size: 3102 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 10 Size: 54 1425 343 228 3243 Fragments arranged by size: 3243 1425 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 3251 3610 3769 518 Size: 648 2085 359 159 2042 Fragments arranged by size: 2085 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2240 2506 2563 2709 3386 4534 4779 Size: 441 193 266 57 146 677 1148 245 Cuts at: 4779 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 441 266 245 193 146 57 NarI GG'CG_CC Cuts at: 2517 2517 Size: 5293 NcoI C'CATG_G Cuts at: 513 1065 2004 2310 513 Size: 552 939 306 3496 Fragments arranged by size: 3496 939 552 306 NdeI CA'TA_TG Cuts at: 387 387 Size: 5293 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5293 NotI GC'GGCC_GC Cuts at: 2740 2740 Size: 5293 NspI r_CATG'y Cuts at: 1132 1229 2651 4196 1132 Size: 97 1422 1545 2229 Fragments arranged by size: 2229 1545 1422 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5293 PciI A'CATG_T Cuts at: 4192 4192 Size: 5293 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5293 PmeI GTTT'AAAC Cuts at: 2597 2597 Size: 5293 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3362 3394 4875 4907 3362 Size: 32 1481 32 3748 Fragments arranged by size: 3748 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5293 PsiI TTA'TAA Cuts at: 2863 2863 Size: 5293 PstI C_TGCA'G Cuts at: 830 2121 2322 2645 830 Size: 1291 201 323 3478 Fragments arranged by size: 3478 1291 323 201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5293 PvuII CAG'CTG Cuts at: 2047 2240 2506 2563 2047 Size: 193 266 57 4777 Fragments arranged by size: 4777 266 193 57 SacI G_AGCT'C Cuts at: 2408 2540 2408 Size: 132 5161 SalI G'TCGA_C Cuts at: 1936 2635 1936 Size: 699 4594 SbfI CC_TGCA'GG Cuts at: 2645 2645 Size: 5293 ScaI AGT'ACT Cuts at: 3541 5253 3541 Size: 1712 3581 SfcI C'TryA_G Cuts at: 826 1045 2117 2318 2641 3776 4457 4648 826 Size: 219 1072 201 323 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 681 323 219 201 191 SfoI GGC'GCC Cuts at: 2518 2518 Size: 5293 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5293 SmaI CCC'GGG Cuts at: 1872 2620 1872 Size: 748 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2584 3397 4298 4560 820 Size: 197 926 641 813 901 262 1553 Fragments arranged by size: 1553 926 901 813 641 262 197 SnaBI TAC'GTA Cuts at: 493 3084 493 Size: 2591 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5293 SphI G_CATG'C Cuts at: 1132 2651 1132 Size: 1519 3774 SspI AAT'ATT Cuts at: 5 52 3217 5 Size: 47 3165 2081 Fragments arranged by size: 3165 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5293 StyI C'CwwG_G Cuts at: 513 1065 2004 2082 2226 2310 2732 513 Size: 552 939 78 144 84 422 3074 Fragments arranged by size: 3074 939 552 422 144 84 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3320 3337 3490 3675 4120 325 Size: 1255 1740 17 153 185 445 1498 Fragments arranged by size: 1740 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3539 5251 96 Size: 274 80 33 51 1443 1562 1712 138 Fragments arranged by size: 1712 1562 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5253 5285 5253 Size: 32 5261 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5293 XbaI T'CTAG_A Cuts at: 2629 2629 Size: 5293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5293 XmaI C'CCGG_G Cuts at: 1870 2618 1870 Size: 748 4545 XmnI GAAnn'nnTTC Cuts at: 3422 3422 Size: 5293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2374 3101 276 Size: 53 83 186 936 840 727 2468 Fragments arranged by size: 2468 936 840 727 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI