Restriction map for pFN21AE1007
 (Linear) (Six-base) MAPSORT of: KIEE1007.seq  Check: 4194  from: 1  to: 588



KIEE1007.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     342     423     588 
   Size:        342      81     165

  Fragments arranged by size: 

                 342     165      81
 
AatII G_ACGT'C

Cuts at:      0     371     588 
   Size:        371     217
 
AccI GT'mk_AC

Cuts at:      0     206     588 
   Size:        206     382
 
AhdI GACnn_n'nnGTC

Cuts at:      0     558     588 
   Size:        558      30
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363     588 
   Size:        363     225
 
AvaI C'yCGr_G

Cuts at:      0     505     588 
   Size:        505      83
 
BanI G'GyrC_C

Cuts at:      0     511     588 
   Size:        511      77
 
BanII G_rGCy'C

Cuts at:      0     403     535     588 
   Size:        403     132      53

  Fragments arranged by size: 

                 403     132      53
 
BbeI G_GCGC'C

Cuts at:      0     515     588 
   Size:        515      73
 
BglI GCCn_nnn'nGGC

Cuts at:      0      61     588 
   Size:         61     527
 
BlpI GC'TnA_GC

Cuts at:      0     493     588 
   Size:        493      95
 
Bme1580I G_kGCm'C

Cuts at:      0     135     588 
   Size:        135     453
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      90     531     588 
   Size:         90     441      57

  Fragments arranged by size: 

                 441      90      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     496     588 
   Size:         64     432      92

  Fragments arranged by size: 

                 432      92      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     298     588 
   Size:        298     290
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     327     588 
   Size:        327     261
 
BsaHI Gr'CG_yC

Cuts at:      0     196     368     512     588 
   Size:        196     172     144      76

  Fragments arranged by size: 

                 196     172     144      76
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     315     478     588 
   Size:        315     163     110

  Fragments arranged by size: 

                 315     163     110
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     376     588 
   Size:        247     129     212

  Fragments arranged by size: 

                 247     212     129
 
BsiHKAI G_wGCw'C

Cuts at:      0     304     403     535     588 
   Size:        304      99     132      53

  Fragments arranged by size: 

                 304     132      99      53
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     304     403     535     588 
   Size:        135     169      99     132      53

  Fragments arranged by size: 

                 169     135     132      99      53
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     320     342     423     588 
   Size:        320      22      81     165

  Fragments arranged by size: 

                 320     165      81      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     354     588 
   Size:        354     234
 
BstEII G'GTnAC_C

Cuts at:      0     252     273     588 
   Size:        252      21     315

  Fragments arranged by size: 

                 315     252      21
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     264     285     588 
   Size:        264      21     303

  Fragments arranged by size: 

                 303     264      21
 
BtgI C'CryG_G

Cuts at:      0     305     521     588 
   Size:        305     216      67

  Fragments arranged by size: 

                 305     216      67
 
BtsI GCAGTG_nn'

Cuts at:      0     218     588 
   Size:        218     370
 
EaeI y'GGCC_r

Cuts at:      0      53     404     572     588 
   Size:         53     351     168      16

  Fragments arranged by size: 

                 351     168      53      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      79      97     588 
   Size:         79      18     491

  Fragments arranged by size: 

                 491      79      18
 
EcoICRI GAG'CTC

Cuts at:      0     401     533     588 
   Size:        401     132      55

  Fragments arranged by size: 

                 401     132      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     496     588 
   Size:         64     432      92

  Fragments arranged by size: 

                 432      92      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     289     316     588 
   Size:        289      27     272

  Fragments arranged by size: 

                 289     272      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     105     294     588 
   Size:        105     189     294

  Fragments arranged by size: 

                 294     189     105
 
FspI TGC'GCA

Cuts at:      0     541     588 
   Size:        541      47
 
HaeII r_GCGC'y

Cuts at:      0     515     588 
   Size:        515      73
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     544     576     588 
   Size:        544      32      12

  Fragments arranged by size: 

                 544      32      12
 
KasI G'GCGC_C

Cuts at:      0     511     588 
   Size:        511      77
 
MscI TGG'CCA

Cuts at:      0      55     588 
   Size:         55     533
 
MspA1I CmG'CkG

Cuts at:      0      42     235     501     558     588 
   Size:         42     193     266      57      30

  Fragments arranged by size: 

                 266     193      57      42      30
 
NarI GG'CG_CC

Cuts at:      0     512     588 
   Size:        512      76
 
NcoI C'CATG_G

Cuts at:      0     305     588 
   Size:        305     283
 
PstI C_TGCA'G

Cuts at:      0     116     317     588 
   Size:        116     201     271

  Fragments arranged by size: 

                 271     201     116
 
PvuII CAG'CTG

Cuts at:      0      42     235     501     558     588 
   Size:         42     193     266      57      30

  Fragments arranged by size: 

                 266     193      57      42      30
 
SacI G_AGCT'C

Cuts at:      0     403     535     588 
   Size:        403     132      53

  Fragments arranged by size: 

                 403     132      53
 
SfcI C'TryA_G

Cuts at:      0     112     313     588 
   Size:        112     201     275

  Fragments arranged by size: 

                 275     201     112
 
SfoI GGC'GCC

Cuts at:      0     513     588 
   Size:        513      75
 
SmlI C'TyrA_G

Cuts at:      0     579     588 
   Size:        579       9
 
StyI C'CwwG_G

Cuts at:      0      77     221     305     588 
   Size:         77     144      84     283

  Fragments arranged by size: 

                 283     144      84      77
 
ZraI GAC'GTC

Cuts at:      0     369     588 
   Size:        369     219
 

 Enzymes that do cut:

     AarI    AatII     AccI     AhdI    AlwNI     AvaI     BanI    BanII
     BbeI     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I     BsaI
    BsaHI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI   BstEII
    BstXI     BtgI     BtsI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FspI    HaeII    Hin4I     KasI     MscI   MspA1I     NarI
     NcoI     PstI    PvuII     SacI     SfcI     SfoI     SmlI     StyI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI    EcoRI    EcoRV     FalI     FseI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI
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