(Circular) (Six-base) MAPSORT of: pFN21AE1005.seq Check: 189 from: 1 to: 5263 pFN21AE1005 5263 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2360 2360 Size: 5263 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3073 278 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2232 2606 1937 Size: 295 374 4594 Fragments arranged by size: 4594 374 295 Acc65I G'GTAC_C Cuts at: 2584 2584 Size: 5263 AclI AA'CG_TT Cuts at: 1563 3390 3763 1563 Size: 1827 373 3063 Fragments arranged by size: 3063 1827 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3326 4710 4978 1632 Size: 1694 1384 268 1917 Fragments arranged by size: 1917 1694 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5066 AflIII A'CryG_T Cuts at: 1203 4162 5139 1203 Size: 2959 977 1327 Fragments arranged by size: 2959 1327 977 AgeI A'CCGG_T Cuts at: 4094 4094 Size: 5263 AhdI GACnn_n'nnGTC Cuts at: 3992 3992 Size: 5263 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5263 AlwNI CAG_nnn'CTG Cuts at: 3006 4578 3006 Size: 1572 3691 ApaLI G'TGCA_C Cuts at: 3320 4476 3320 Size: 1156 4107 ApoI r'AATT_y Cuts at: 1493 2501 2572 2798 2950 5059 1493 Size: 1008 71 226 152 2109 1697 Fragments arranged by size: 2109 1697 1008 226 152 71 AseI AT'TA_AT Cuts at: 160 1032 3817 160 Size: 872 2785 1606 Fragments arranged by size: 2785 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 AvaI C'yCGr_G Cuts at: 1870 1943 2031 2204 2399 2588 1870 Size: 73 88 173 195 189 4545 Fragments arranged by size: 4545 195 189 173 88 73 BamHI G'GATC_C Cuts at: 2250 2593 2960 2250 Size: 343 367 4553 Fragments arranged by size: 4553 367 343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2584 4039 618 Size: 325 217 627 288 509 1455 1842 Fragments arranged by size: 1842 1455 627 509 325 288 217 BanII G_rGCy'C Cuts at: 1391 2582 1391 Size: 1191 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2379 2418 928 Size: 971 480 39 3773 Fragments arranged by size: 3773 971 480 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3454 3488 1903 Size: 34 50 34 1433 34 3678 Fragments arranged by size: 3678 1433 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3155 4365 3155 Size: 1210 4053 BclI T'GATC_A Cuts at: 1548 1548 Size: 5263 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 2339 3874 Size: 107 122 71 631 605 355 312 1535 Cuts at: 3874 136 Size: 1525 Fragments arranged by size: 1535 1525 631 605 355 312 122 107 71 BglII A'GATC_T Cuts at: 5258 5258 Size: 5263 BlpI GC'TnA_GC Cuts at: 2680 2680 Size: 5263 Bme1580I G_kGCm'C Cuts at: 1790 2078 2102 3324 4480 1790 Size: 288 24 1222 1156 2573 Fragments arranged by size: 2573 1222 1156 288 24 BmgBI CAC'GTC Cuts at: 1318 2212 1318 Size: 894 4369 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2249 2994 3952 Size: 416 53 83 399 24 832 745 958 Cuts at: 3952 442 Size: 1753 Fragments arranged by size: 1753 958 832 745 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5263 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2426 3923 1239 Size: 1187 1497 2579 Fragments arranged by size: 2579 1497 1187 Bpu10I CC'TnA_GC Cuts at: 2239 2239 Size: 5263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3388 4253 4551 3388 Size: 865 298 4100 Fragments arranged by size: 4100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3926 882 Size: 617 2427 2219 Fragments arranged by size: 2427 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2536 3054 493 Size: 711 1332 518 2702 Fragments arranged by size: 2702 1332 711 518 BsaBI GATnn'nnATC Cuts at: 5257 5257 Size: 5263 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3070 3452 4087 275 Size: 53 83 186 936 1537 382 635 1451 Fragments arranged by size: 1537 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2482 3696 4094 4368 4515 4926 2482 Size: 1214 398 274 147 411 2819 Fragments arranged by size: 2819 1214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5038 Fragments arranged by size: 5038 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2385 2417 2429 1186 Size: 1006 193 32 12 4020 Fragments arranged by size: 4020 1006 193 32 12 BseYI C'CCAG_C Cuts at: 2128 2295 2458 4466 4962 2128 Size: 167 163 2008 496 2429 Fragments arranged by size: 2429 2008 496 167 163 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4690 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2713 3474 4502 1540 Size: 275 186 712 761 1028 2301 Fragments arranged by size: 2301 1028 761 712 275 186 BsiHKAI G_wGCw'C Cuts at: 3324 3409 4480 3324 Size: 85 1071 4107 Fragments arranged by size: 4107 1071 85 BsmI GAATG_Cn' Cuts at: 2774 2867 2774 Size: 93 5170 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2102 2582 3324 3409 4480 1391 Size: 399 288 24 480 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 288 85 24 BspEI T'CCGG_A Cuts at: 4926 4926 Size: 5263 BspHI T'CATG_A Cuts at: 3152 4882 3152 Size: 1730 3533 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2360 2618 844 Size: 1516 258 3489 Fragments arranged by size: 3489 1516 258 BsrBI CCG'CTC Cuts at: 1394 1823 2200 2238 2498 3150 1394 Size: 429 377 38 260 652 3507 Fragments arranged by size: 3507 652 429 377 260 38 BsrDI GCAATG_nn' Cuts at: 65 1248 3758 3932 65 Size: 1183 2510 174 1396 Fragments arranged by size: 2510 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3907 4094 1700 Size: 110 2097 187 2869 Fragments arranged by size: 2869 2097 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5263 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5263 BssSI C'ACGA_G Cuts at: 3323 4335 3323 Size: 1012 4251 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5263 BstBI TT'CG_AA Cuts at: 4067 4067 Size: 5263 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5263 BstYI r'GATC_y Cuts at: 1972 2250 2383 2593 2960 3345 3362 4814 4900 Size: 278 133 210 367 385 17 1452 86 Cuts at: 4900 4912 5258 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 278 210 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 2302 513 Size: 552 960 277 3474 Fragments arranged by size: 3474 960 552 277 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4524 Fragments arranged by size: 4524 683 56 BtsI GCAGTG_nn' Cuts at: 2779 3573 3593 2779 Size: 794 20 4449 Fragments arranged by size: 4449 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5263 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5228 DraI TTT'AAA Cuts at: 2567 2567 Size: 5263 DraIII CAC_nnn'GTG Cuts at: 2082 2308 2082 Size: 226 5037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4270 809 Size: 507 2954 1802 Fragments arranged by size: 2954 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2542 2710 Size: 54 1425 50 275 18 186 526 168 Cuts at: 2710 3599 5228 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 526 275 186 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2710 1537 Size: 275 898 4090 Fragments arranged by size: 4090 898 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3193 1353 Size: 1840 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3850 4224 4370 387 Size: 1064 138 2261 374 146 1280 Fragments arranged by size: 2261 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2426 3326 3923 4710 4978 1239 Size: 393 794 900 597 787 268 1524 Fragments arranged by size: 1524 900 794 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2441 2441 Size: 5263 EcoO109I rG'GnC_Cy Cuts at: 2322 2322 Size: 5263 EcoRI G'AATT_C Cuts at: 2501 2572 2950 2501 Size: 71 378 4814 Fragments arranged by size: 4814 378 71 FspI TGC'GCA Cuts at: 3769 3769 Size: 5263 HaeII r_GCGC'y Cuts at: 1678 2129 2302 4410 1678 Size: 451 173 2108 2531 Fragments arranged by size: 2531 2108 451 173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3904 3936 3978 4010 1373 Size: 32 135 32 2332 32 42 32 2626 Fragments arranged by size: 2626 2332 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2607 2853 669 Size: 988 281 669 246 3079 Fragments arranged by size: 3079 988 669 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5263 HpaI GTT'AAC Cuts at: 2853 2853 Size: 5263 KpnI G_GTAC'C Cuts at: 2588 2588 Size: 5263 MfeI C'AATT_G Cuts at: 2862 2862 Size: 5263 MluI A'CGCG_T Cuts at: 5139 5139 Size: 5263 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4377 4561 1305 Size: 3072 184 2007 Fragments arranged by size: 3072 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2544 10 Size: 54 1425 343 712 2729 Fragments arranged by size: 2729 1425 712 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2361 3221 3580 3739 518 Size: 648 1195 860 359 159 2042 Fragments arranged by size: 2042 1195 860 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2248 2679 3356 4504 4749 1606 Size: 642 431 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 431 245 NcoI C'CATG_G Cuts at: 513 1065 2025 513 Size: 552 960 3751 Fragments arranged by size: 3751 960 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5263 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5263 NotI GC'GGCC_GC Cuts at: 2710 2710 Size: 5263 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5263 NspI r_CATG'y Cuts at: 1132 1229 2012 2422 2621 4166 1132 Size: 97 783 410 199 1545 2229 Fragments arranged by size: 2229 1545 783 410 199 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5263 PciI A'CATG_T Cuts at: 4162 4162 Size: 5263 PflMI CCAn_nnn'nTGG Cuts at: 1108 2261 2308 1108 Size: 1153 47 4063 Fragments arranged by size: 4063 1153 47 PfoI T'CCnGG_A Cuts at: 2442 2442 Size: 5263 PmeI GTTT'AAAC Cuts at: 2567 2567 Size: 5263 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3332 3364 4845 4877 3332 Size: 32 1481 32 3718 Fragments arranged by size: 3718 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5263 PsiI TTA'TAA Cuts at: 2833 2833 Size: 5263 PstI C_TGCA'G Cuts at: 830 2064 2615 830 Size: 1234 551 3478 Fragments arranged by size: 3478 1234 551 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5263 SalI G'TCGA_C Cuts at: 1936 2605 1936 Size: 669 4594 SbfI CC_TGCA'GG Cuts at: 2615 2615 Size: 5263 ScaI AGT'ACT Cuts at: 3511 5223 3511 Size: 1712 3551 SexAI A'CCwGG_T Cuts at: 2078 2078 Size: 5263 SfcI C'TryA_G Cuts at: 826 1045 2060 2611 3746 4427 4618 826 Size: 219 1015 551 1135 681 191 1471 Fragments arranged by size: 1471 1135 1015 681 551 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5263 SmaI CCC'GGG Cuts at: 1872 2033 2206 2590 1872 Size: 161 173 384 4545 Fragments arranged by size: 4545 384 173 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3367 4268 4530 820 Size: 197 926 1424 901 262 1553 Fragments arranged by size: 1553 1424 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3054 493 Size: 2561 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5263 SphI G_CATG'C Cuts at: 1132 2621 1132 Size: 1489 3774 SspI AAT'ATT Cuts at: 5 52 3187 5 Size: 47 3135 2081 Fragments arranged by size: 3135 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5263 StyI C'CwwG_G Cuts at: 513 1065 2025 2702 513 Size: 552 960 677 3074 Fragments arranged by size: 3074 960 677 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3290 3307 3460 3645 4090 325 Size: 1255 1710 17 153 185 445 1498 Fragments arranged by size: 1710 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3509 5221 96 Size: 274 80 33 51 1443 1532 1712 138 Fragments arranged by size: 1712 1532 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5223 5255 5223 Size: 32 5231 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5263 XbaI T'CTAG_A Cuts at: 2599 2599 Size: 5263 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5263 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5263 XmaI C'CCGG_G Cuts at: 1870 2031 2204 2588 1870 Size: 161 173 384 4545 Fragments arranged by size: 4545 384 173 161 XmnI GAAnn'nnTTC Cuts at: 3392 3392 Size: 5263 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3071 276 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI