Restriction map for
pFN21AB5360
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BcgI BclI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII CfoI Cfr10I
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 837 CGCG CG^CG
AlwNI 891 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 856 GGCACC G^G[CT][GA]CC
BbvCI 342 CCTCAGC CCTCAGC(-5/-2)
BciVI 468 GGATAC GTATCC(6/5)
BcnI 157 CCGGG CC^[GC]GG
BfaI 589 CTAG C^TAG
BpmI 279 CTGGAG CTGGAG(16/14)
BsaBI 360 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 806 CTCCTC GAGGAG(10/8)
BsiEI 475 CGATCG CG[GA][CT]^CG
BspDI 779 ATCGAT AT^CGAT
BstUI 837 CGCG CG^CG
BstXI 542 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 874 GGATCT [GA]^GATC[CT]
Cac8I 958 GCTTGC GC[ACGT]^[ACGT]GC
ClaI 779 ATCGAT AT^CGAT
DsaI 542 CCATGG C^C[GA][CT]GG
EarI 1078 GAAGAG CTCTTC(1/4)
EcoRI 607 GAATTC G^AATTC
EcoT14I 542 CCATGG C^C[AT][AT]GG
FauI 748 CCCGC CCCGC(4/6)
HapII 157 CCGG C^CGG
HpaII 157 CCGG C^CGG
HphI 764 TCACC GGTGA(8/7)
Ksp632I 1078 GAAGAG CTCTTC(1/4)
MaeI 589 CTAG C^TAG
MaeII 530 ACGT A^CGT
MamI 360 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 1078 GAAGA GAAGA(8/7)
MflI 874 GGATCT [GA]^GATC[CT]
MspA1I 423 CAGCTG C[AC]G^C[GT]G
MspI 157 CCGG C^CGG
MvnI 837 CGCG CG^CG
NciI 157 CCGGG CC^[GC]GG
NcoI 542 CCATGG C^CATGG
NspI 47 GCATGT [GA]CATG^[CT]
PpuMI 338 GGGACCT [GA]G^G[AT]CC[CT]
PstI 560 CTGCAG CTGCA^G
PvuI 475 CGATCG CGAT^CG
PvuII 423 CAGCTG CAG^CTG
SapI 1078 GAAGAGC GCTCTTC(1/4)
StyI 542 CCATGG C^C[AT][AT]GG
Tsp45I 284 GTGAC ^GT[GC]AC
XhoII 874 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 749 CCGC CCGC(-3/-1)
823 GCGG
AcsI 607 GAATTC [GA]^AATT[CT]
1027 GAATTT
AocI 20 CCTCAGG CC^T[ACGT]AGG
808 CCTCAGG
ApoI 607 GAATTC [GA]^AATT[CT]
1027 GAATTT
AvaII 41 GGACC G^G[AT]CC
339 GGACC
BbvI 1072 GCAGC GCAGC(8/12)
200 GCTGC
BstF5I 396 CATCC GGATG(2/0)
844 CATCC
BstNI 696 CCTGG CC^[AT]GG
1091 CCAGG
Bsu36I 20 CCTCAGG CC^T[ACGT]AGG
808 CCTCAGG
DraII 338 GGGACCT [GA]G^G[ACGT]CC[CT]
569 GGGGCCT
Eco81I 20 CCTCAGG CC^T[ACGT]AGG
808 CCTCAGG
EcoO109I 338 GGGACCT [GA]G^G[ACGT]CC[CT]
569 GGGGCCT
EcoRII 696 CCTGG ^CC[AT]GG
1091 CCAGG
Fnu4HI 200 GCTGC GC^[ACGT]GC
1072 GCAGC
FokI 396 CATCC GGATG(9/13)
844 CATCC
HaeIII 260 GGCC GG^CC
571 GGCC
ItaI 200 GCTGC GC^[ACGT]GC
1072 GCAGC
MslI 543 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
643 CATCGGTGTG
MvaI 696 CCTGG CC^[AT]GG
1091 CCAGG
PflMI 329 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
395 CCATCCATTGG
TseI 200 GCTGC G^C[AT]GC
1072 GCAGC
Van91I 329 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
395 CCATCCATTGG
XcmI 542 CCATGGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
843 CCATCCTGTTCATGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BsmFI BsrI BssKI Cfr13I DdeI
DpnII DraI HinfI MaeIII MboI
MnlI MseI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I Tsp509I
TspRI TthHB8I