Restriction map for pFN21AB8730
 (Linear) (Six-base) MAPSORT of: KIBB8730.seq  Check: 2359  from: 1  to: 1179



KIBB8730.orf

 With 182 enzymes: SgfI * 

                        April 2, 2009 17:32  ..

AccI GT'mk_AC

Cuts at:      0    1126    1179 
   Size:       1126      53
 
AclI AA'CG_TT

Cuts at:      0      89    1179 
   Size:         89    1090
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     973    1126    1179 
   Size:        185     788     153      53

  Fragments arranged by size: 

                 788     185     153      53
 
AfeI AGC'GCT

Cuts at:      0     541    1009    1179 
   Size:        541     468     170

  Fragments arranged by size: 

                 541     468     170
 
AflIII A'CryG_T

Cuts at:      0     707    1179 
   Size:        707     472
 
AlwNI CAG_nnn'CTG

Cuts at:      0     205     722    1179 
   Size:        205     517     457

  Fragments arranged by size: 

                 517     457     205
 
ApoI r'AATT_y

Cuts at:      0     597    1179 
   Size:        597     582
 
BanI G'GyrC_C

Cuts at:      0     460     651    1179 
   Size:        460     191     528

  Fragments arranged by size: 

                 528     460     191
 
BanII G_rGCy'C

Cuts at:      0      35    1092    1179 
   Size:         35    1057      87

  Fragments arranged by size: 

                1057      87      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     173     779    1179 
   Size:        173     606     400

  Fragments arranged by size: 

                 606     400     173
 
BbvCI CC'TCA_GC

Cuts at:      0     572    1179 
   Size:        572     607
 
Bme1580I G_kGCm'C

Cuts at:      0     656    1179 
   Size:        656     523
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     802    1179 
   Size:        802     377
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     561     693    1106    1179 
   Size:        561     132     413      73

  Fragments arranged by size: 

                 561     413     132      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     762    1080    1179 
   Size:        762     318      99

  Fragments arranged by size: 

                 762     318      99
 
Bpu10I CC'TnA_GC

Cuts at:      0     572    1179 
   Size:        572     607
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1033    1179 
   Size:       1033     146
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     833    1179 
   Size:        833     346
 
BsaAI yAC'GTr

Cuts at:      0     374    1179 
   Size:        374     805
 
BsaWI w'CCGG_w

Cuts at:      0     740    1179 
   Size:        740     439
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     922     952    1179 
   Size:         28     894      30     227

  Fragments arranged by size: 

                 894     227      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     560     930    1179 
   Size:         19     541     370     249

  Fragments arranged by size: 

                 541     370     249      19
 
BseYI C'CCAG_C

Cuts at:      0     947    1063    1179 
   Size:        947     116     116

  Fragments arranged by size: 

                 947     116     116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     239     448    1179 
   Size:        209      30     209     731

  Fragments arranged by size: 

                 731     209     209      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     910     921    1179 
   Size:        910      11     258

  Fragments arranged by size: 

                 910     258      11
 
BsmI GAATG_Cn'

Cuts at:      0     200     342    1179 
   Size:        200     142     837

  Fragments arranged by size: 

                 837     200     142
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     656     910     921    1092    1179 
   Size:         35     621     254      11     171      87

  Fragments arranged by size: 

                 621     254     171      87      35      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     427    1179 
   Size:        427     752
 
BsrDI GCAATG_nn'

Cuts at:      0     122    1179 
   Size:        122    1057
 
BsrFI r'CCGG_y

Cuts at:      0     243     842    1179 
   Size:        243     599     337

  Fragments arranged by size: 

                 599     337     243
 
BssSI C'ACGA_G

Cuts at:      0     889    1179 
   Size:        889     290
 
BstYI r'GATC_y

Cuts at:      0      18     992    1179 
   Size:         18     974     187

  Fragments arranged by size: 

                 974     187      18
 
Bsu36I CC'TnA_GG

Cuts at:      0     668    1179 
   Size:        668     511
 
BtgI C'CryG_G

Cuts at:      0     476     996    1179 
   Size:        476     520     183

  Fragments arranged by size: 

                 520     476     183
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     569    1179 
   Size:        569     610
 
BtsI GCAGTG_nn'

Cuts at:      0     919    1179 
   Size:        919     260
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     357    1179 
   Size:        357     822
 
EaeI y'GGCC_r

Cuts at:      0     410     479    1179 
   Size:        410      69     700

  Fragments arranged by size: 

                 700     410      69
 
EarI CTCTTCn'nnn_

Cuts at:      0     851    1179 
   Size:        851     328
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     762     973    1080    1126    1179 
   Size:        185     577     211     107      46      53

  Fragments arranged by size: 

                 577     211     185     107      53      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1165    1179 
   Size:       1165      14
 
HaeII r_GCGC'y

Cuts at:      0     525     543    1011    1179 
   Size:        525      18     468     168

  Fragments arranged by size: 

                 525     468     168      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1179 
   Size:         30    1149
 
MscI TGG'CCA

Cuts at:      0     412     481    1179 
   Size:        412      69     698

  Fragments arranged by size: 

                 698     412      69
 
MspA1I CmG'CkG

Cuts at:      0     432    1179 
   Size:        432     747
 
NcoI C'CATG_G

Cuts at:      0     476    1179 
   Size:        476     703
 
NspI r_CATG'y

Cuts at:      0     711    1179 
   Size:        711     468
 
PasI CC'CwG_GG

Cuts at:      0     901    1179 
   Size:        901     278
 
PciI A'CATG_T

Cuts at:      0     707    1179 
   Size:        707     472
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     117     970    1179 
   Size:        117     853     209

  Fragments arranged by size: 

                 853     209     117
 
PshAI GACnn'nnGTC

Cuts at:      0     173    1179 
   Size:        173    1006
 
PvuII CAG'CTG

Cuts at:      0     432    1179 
   Size:        432     747
 
SgrAI Cr'CCGG_yG

Cuts at:      0     243    1179 
   Size:        243     936
 
SmlI C'TyrA_G

Cuts at:      0    1048    1179 
   Size:       1048     131
 
SspI AAT'ATT

Cuts at:      0     979    1179 
   Size:        979     200
 
StuI AGG'CCT

Cuts at:      0    1039    1179 
   Size:       1039     140
 
StyI C'CwwG_G

Cuts at:      0     476    1040    1179 
   Size:        476     564     139

  Fragments arranged by size: 

                 564     476     139
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1093    1125    1179 
   Size:       1093      32      54

  Fragments arranged by size: 

                1093      54      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     266     765    1179 
   Size:        266     499     414

  Fragments arranged by size: 

                 499     414     266
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI   AflIII    AlwNI     ApoI     BanI
    BanII     BbsI    BbvCI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BssSI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EarI  Eco57MI
 EcoO109I    HaeII    Hin4I     MscI   MspA1I     NcoI     NspI     PasI
     PciI    PflMI    PshAI    PvuII    SgrAI     SmlI     SspI     StuI
     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI    BsaBI    BsaHI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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