Restriction map for
pFN21AE1468
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BciVI BfrI BglI BglII
BlnI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MunI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1543 GACGTC GACGT^C
AccIII 1692 TCCGGA T^CCGGA
AcsI 2378 AAATTC [GA]^AATT[CT]
AflIII 713 ACGTGT A^C[GA][CT]GT
ApoI 2378 AAATTC [GA]^AATT[CT]
Asp700I 1300 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 556 GGCGCC GGCGC^C
BbrPI 712 CACGTG CAC^GTG
BfaI 1009 CTAG C^TAG
BlpI 38 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 38 GCTCAGC GC^T[ACGT]AGC
BsaAI 712 CACGTG [CT]AC^GT[GA]
BsaI 1678 GAGACC GGTCTC(1/5)
BsaWI 1692 TCCGGA [AT]^CCGG[AT]
BseAI 1692 TCCGGA T^CCGGA
BsiEI 652 CGGCCG CG[GA][CT]^CG
BspEI 1692 TCCGGA T^CCGGA
BsrDI 699 GCAATG GCAATG(2/0)
BsrFI 654 GCCGGC [GA]^CCGG[CT]
BstEII 2703 GGTGACC G^GT[ACGT]ACC
BstPI 2703 GGTGACC G^GT[ACGT]ACC
BstXI 353 CCAACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 38 GCTCAGC GC^T[ACGT]AGC
Cfr10I 654 GCCGGC [GA]^CCGG[CT]
DraIII 1918 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 131 CCGTGG C^C[GA][CT]GG
EagI 652 CGGCCG C^GGCCG
EclXI 652 CGGCCG C^GGCCG
Eco52I 652 CGGCCG C^GGCCG
EcoNI 1485 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2703 GGTGACC G^GT[ACGT]ACC
HaeII 556 GGCGCC [GA]GCGC^[CT]
HincII 1393 GTCAAC GT[CT]^[GA]AC
HindII 1393 GTCAAC GT[CT]^[GA]AC
HindIII 121 AAGCTT A^AGCTT
KasI 556 GGCGCC G^GCGCC
MaeI 1009 CTAG C^TAG
MroI 1692 TCCGGA T^CCGGA
NaeI 654 GCCGGC GCC^GGC
NarI 556 GGCGCC GG^CGCC
NdeI 563 CATATG CA^TATG
NgoMIV 654 GCCGGC G^CCGGC
PmaCI 712 CACGTG CAC^GTG
PmlI 712 CACGTG CAC^GTG
PpuMI 499 AGGACCC [GA]G^G[AT]CC[CT]
SfoI 556 GGCGCC GGC^GCC
SphI 1014 GCATGC GCATG^C
TfiI 1530 GATTC G^A[AT]TC
XcmI 2519 CCAGCTGGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1300 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 1921 CGCG CG^CG
2459 CGCG
Alw44I 1240 GTGCAC G^TGCAC
2769 GTGCAC
ApaLI 1240 GTGCAC G^TGCAC
2769 GTGCAC
BbsI 81 GAAGAC GAAGAC(2/6)
1619 GTCTTC
BcgI 296 GCATTTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2718 GCAGCCTGTTCG
BclI 342 TGATCA T^GATCA
686 TGATCA
BpuAI 81 GAAGAC GAAGAC(2/6)
1619 GTCTTC
BspMI 2615 ACCTGC ACCTGC(4/8)
2762 ACCTGC
BsrBI 1807 CCGCTC CCGCTC(-3/-3)
2278 GAGCGG
BssSI 971 CTCGTG CACGAG(-5/-1)
2385 CTCGTG
BstUI 1921 CGCG CG^CG
2459 CGCG
FauI 579 CCCGC CCCGC(4/6)
2460 GCGGG
FbaI 342 TGATCA T^GATCA
686 TGATCA
MaeIII 1598 GTGAC ^GT[ACGT]AC
2704 GTGAC
MseI 629 TTAA T^TAA
2603 TTAA
MvnI 1921 CGCG CG^CG
2459 CGCG
SmaI 1100 CCCGGG CCC^GGG
2540 CCCGGG
StuI 609 AGGCCT AGG^CCT
2444 AGGCCT
Tru9I 629 TTAA T^TAA
2603 TTAA
Tsp45I 1598 GTGAC ^GT[GC]AC
2704 GTGAC
XmaCI 1100 CCCGGG C^CCGGG
2540 CCCGGG
XmaI 1100 CCCGGG C^CCGGG
2540 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp509I TspRI
TthHB8I Van91I XhoII