Restriction map for
pFN21AE1553
(Circular) (Six-base) MAPSORT of: pFN21AE1553.seq Check: 5845 from: 1 to: 6601
pFN21AE1553 6601 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4411 278
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3944 1937
Size: 2007 4594
Acc65I G'GTAC_C
Cuts at: 3922 3922
Size: 6601
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 4664 6048 6316 1632
Size: 876 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 876 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 2268 3079 5500 6477 1203
Size: 1065 811 2421 977 1327
Fragments arranged by size:
2421 1327 1065 977 811
AgeI A'CCGG_T
Cuts at: 5432 5432
Size: 6601
AhdI GACnn_n'nnGTC
Cuts at: 5330 5330
Size: 6601
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2781 2813 2781
Size: 32 6569
AlwNI CAG_nnn'CTG
Cuts at: 2767 3570 4344 5916 2767
Size: 803 774 1572 3452
Fragments arranged by size:
3452 1572 803 774
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 2464 3910 4136 4288 6397 1493
Size: 971 1446 226 152 2109 1697
Fragments arranged by size:
2109 1697 1446 971 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5155 160
Size: 872 4123 1606
Fragments arranged by size:
4123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 2841 2882 3926 1870
Size: 73 898 41 1044 4545
Fragments arranged by size:
4545 1044 898 73 41
BamHI G'GATC_C
Cuts at: 3931 4298 3931
Size: 367 6234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 3922 5377 618
Size: 325 217 627 318 1817 1455 1842
Fragments arranged by size:
1842 1817 1455 627 325 318 217
BanII G_rGCy'C
Cuts at: 1391 3122 3342 3507 3920 1391
Size: 1731 220 165 413 4072
Fragments arranged by size:
4072 1731 413 220 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3519 928
Size: 971 1620 4010
Fragments arranged by size:
4010 1620 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3262 3296 4792 4826 1903
Size: 34 1325 34 1496 34 3678
Fragments arranged by size:
3678 1496 1325 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 5703 4493
Size: 1210 5391
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5212 136
Size: 107 122 71 631 605 337 3203 1525
Fragments arranged by size:
3203 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6596 6596
Size: 6601
BlpI GC'TnA_GC
Cuts at: 4018 4018
Size: 6601
Bme1580I G_kGCm'C
Cuts at: 1790 2341 4662 5818 1790
Size: 551 2321 1156 2573
Fragments arranged by size:
2573 2321 1156 551
BmgBI CAC'GTC
Cuts at: 1318 3080 1318
Size: 1762 4839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 2556 3558
Size: 416 53 83 399 24 883 256 1002
Cuts at: 3558 4332 5290 442
Size: 774 958 1753
Fragments arranged by size:
1753 1002 958 883 774 416 399 256
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3210 3210
Size: 6601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5261 1239
Size: 4022 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 4726 5591 5889 3005
Size: 1721 865 298 3717
Fragments arranged by size:
3717 1721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5264 882
Size: 617 3765 2219
Fragments arranged by size:
3765 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2269 4392 493
Size: 711 1065 2123 2702
Fragments arranged by size:
2702 2123 1065 711
BsaBI GATnn'nnATC
Cuts at: 2708 6595 2708
Size: 3887 2714
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4408 4790 5425 275
Size: 53 83 186 936 2875 382 635 1451
Fragments arranged by size:
2875 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5034 5432 5706 5853 6264 5034
Size: 398 274 147 411 5371
Fragments arranged by size:
5371 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6376
Fragments arranged by size:
6376 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2900 3002 3199 3360 1186
Size: 1714 102 197 161 4427
Fragments arranged by size:
4427 1714 197 161 102
BseYI C'CCAG_C
Cuts at: 3526 5804 6300 3526
Size: 2278 496 3827
Fragments arranged by size:
3827 2278 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 2451 1163
Size: 573 324 391 5313
Fragments arranged by size:
5313 573 391 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 4051 4812 5840 1540
Size: 275 186 664 1386 761 1028 2301
Fragments arranged by size:
2301 1386 1028 761 664 275 186
BsiHKAI G_wGCw'C
Cuts at: 4662 4747 5818 4662
Size: 85 1071 5445
Fragments arranged by size:
5445 1071 85
BsmI GAATG_Cn'
Cuts at: 4112 4205 4112
Size: 93 6508
BsmBI CGTCTCn'nnnn_
Cuts at: 2652 2747 2652
Size: 95 6506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2341 3122 3342 3507 3920 4662 4747
Size: 399 551 781 220 165 413 742 85
Cuts at: 4747 5818 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 781 742 551 413 399 220
165 85
BspEI T'CCGG_A
Cuts at: 6264 6264
Size: 6601
BspHI T'CATG_A
Cuts at: 3226 4490 6220 3226
Size: 1264 1730 3607
Fragments arranged by size:
3607 1730 1264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 3956 844
Size: 1238 1874 3489
Fragments arranged by size:
3489 1874 1238
BsrBI CCG'CTC
Cuts at: 1394 1823 2424 4488 1394
Size: 429 601 2064 3507
Fragments arranged by size:
3507 2064 601 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2722 3160 5096 5270 65
Size: 1183 1474 438 1936 174 1396
Fragments arranged by size:
1936 1474 1396 1183 438 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2664 3140 5245 5432 1700
Size: 110 854 476 2105 187 2869
Fragments arranged by size:
2869 2105 854 476 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6601
BssSI C'ACGA_G
Cuts at: 4661 5673 4661
Size: 1012 5589
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6601
BstBI TT'CG_AA
Cuts at: 2410 5405 2410
Size: 2995 3606
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6601
BstYI r'GATC_y
Cuts at: 1972 2354 2557 3931 4298 4683 4700 6152 6238
Size: 382 203 1374 367 385 17 1452 86
Cuts at: 6238 6250 6596 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1374 385 382 367 346 203
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2187 3605 513
Size: 552 1122 1418 3509
Fragments arranged by size:
3509 1418 1122 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5862
Fragments arranged by size:
5862 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3444 4117 4911 4931 2478
Size: 966 673 794 20 4148
Fragments arranged by size:
4148 966 794 673 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3594 3629 529
Size: 35 3030 35 3501
Fragments arranged by size:
3501 3030 35 35
DraI TTT'AAA
Cuts at: 2380 3905 2380
Size: 1525 5076
DraIII CAC_nnn'GTG
Cuts at: 3566 3566
Size: 6601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5608 809
Size: 507 4292 1802
Fragments arranged by size:
4292 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 2662 4048
Size: 54 1425 50 275 18 487 345 1386
Cuts at: 4048 4937 6566 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1386 889 487 345 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2662 4048 1537
Size: 275 850 1386 4090
Fragments arranged by size:
4090 1386 850 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 4531 1353
Size: 779 2399 3423
Fragments arranged by size:
3423 2399 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5188 5562 5708 387
Size: 1064 138 3599 374 146 1280
Fragments arranged by size:
3599 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 4664 5261 6048 6316 1239
Size: 393 876 2156 597 787 268 1524
Fragments arranged by size:
2156 1524 876 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2821 2821
Size: 6601
EcoRI G'AATT_C
Cuts at: 3910 4288 3910
Size: 378 6223
FspI TGC'GCA
Cuts at: 2912 5107 2912
Size: 2195 4406
HaeII r_GCGC'y
Cuts at: 1678 2015 2146 5748 1678
Size: 337 131 3602 2531
Fragments arranged by size:
3602 2531 337 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5242 5274 5316 5348 1373
Size: 32 135 32 3670 32 42 32 2626
Fragments arranged by size:
3670 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2698 3945 4191 669
Size: 988 281 760 1247 246 3079
Fragments arranged by size:
3079 1247 988 760 281 246
HindIII A'AGCT_T
Cuts at: 748 3480 748
Size: 2732 3869
HpaI GTT'AAC
Cuts at: 4191 4191
Size: 6601
KpnI G_GTAC'C
Cuts at: 3926 3926
Size: 6601
MfeI C'AATT_G
Cuts at: 4200 4200
Size: 6601
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2650 3567 5715 5899 1305
Size: 1345 917 2148 184 2007
Fragments arranged by size:
2148 2007 1345 917 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4779
Fragments arranged by size:
4779 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4559 4918 5077 518
Size: 648 3393 359 159 2042
Fragments arranged by size:
3393 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 3115 3162 3653 4017 4694 5842 6087
Size: 610 899 47 491 364 677 1148 245
Cuts at: 6087 1606
Size: 2120
Fragments arranged by size:
2120 1148 899 677 610 491 364 245
47
NaeI GCC'GGC
Cuts at: 2666 3142 2666
Size: 476 6125
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6049
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6601
NgoMIV G'CCGG_C
Cuts at: 2664 3140 2664
Size: 476 6125
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 3824 3959 5504 1132
Size: 97 2595 135 1545 2229
Fragments arranged by size:
2595 2229 1545 135 97
PasI CC'CwG_GG
Cuts at: 1348 3170 1348
Size: 1822 4779
PciI A'CATG_T
Cuts at: 5500 5500
Size: 6601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3553 3570 1108
Size: 2445 17 4139
Fragments arranged by size:
4139 2445 17
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3419 1613
Size: 1806 4795
PsiI TTA'TAA
Cuts at: 4171 4171
Size: 6601
PstI C_TGCA'G
Cuts at: 830 2215 3521 3953 830
Size: 1385 1306 432 3478
Fragments arranged by size:
3478 1385 1306 432
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
PvuII CAG'CTG
Cuts at: 2216 3162 3653 2216
Size: 946 491 5164
Fragments arranged by size:
5164 946 491
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 3474 4849 6561 3474
Size: 1375 1712 3514
Fragments arranged by size:
3514 1712 1375
SfcI C'TryA_G
Cuts at: 826 1045 2211 2833 3517 3949 5084 5765 5956
Size: 219 1166 622 684 432 1135 681 191
Cuts at: 5956 826
Size: 1471
Fragments arranged by size:
1471 1166 1135 684 681 622 432 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 3928 1872
Size: 2056 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2984 4705 5606 5868 820
Size: 197 926 1041 1721 901 262 1553
Fragments arranged by size:
1721 1553 1041 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 3959 1132
Size: 2827 3774
SspI AAT'ATT
Cuts at: 5 52 4525 5
Size: 47 4473 2081
Fragments arranged by size:
4473 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6601
StyI C'CwwG_G
Cuts at: 513 1065 2033 2498 4040 513
Size: 552 968 465 1542 3074
Fragments arranged by size:
3074 1542 968 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3577 4628 4645 4798 4983 5428 325
Size: 1255 1997 1051 17 153 185 445 1498
Fragments arranged by size:
1997 1498 1255 1051 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3472 3803 4847
Size: 274 80 33 51 1443 1495 331 1044
Cuts at: 4847 6559 96
Size: 1712 138
Fragments arranged by size:
1712 1495 1443 1044 331 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6561 6593 6561
Size: 32 6569
Tth111I GACn'n_nGTC
Cuts at: 1316 2976 1316
Size: 1660 4941
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3550 3567 3550
Size: 17 6584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6601
XmaI C'CCGG_G
Cuts at: 1870 3926 1870
Size: 2056 4545
XmnI GAAnn'nnTTC
Cuts at: 2493 4730 2493
Size: 2237 4364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4409 276
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI