(Linear) (Six-base) MAPSORT of: KIEE1553.seq Check: 3505 from: 1 to: 1896 KIEE1553.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 503 1896 Size: 503 1393 AflIII A'CryG_T Cuts at: 0 263 1074 1896 Size: 263 811 822 Fragments arranged by size: 822 811 263 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 776 808 1896 Size: 776 32 1088 Fragments arranged by size: 1088 776 32 AlwNI CAG_nnn'CTG Cuts at: 0 762 1565 1896 Size: 762 803 331 Fragments arranged by size: 803 762 331 ApoI r'AATT_y Cuts at: 0 459 1896 Size: 459 1437 AvaI C'yCGr_G Cuts at: 0 836 877 1896 Size: 836 41 1019 Fragments arranged by size: 1019 836 41 BanI G'GyrC_C Cuts at: 0 100 1896 Size: 100 1796 BanII G_rGCy'C Cuts at: 0 1117 1337 1502 1896 Size: 1117 220 165 394 Fragments arranged by size: 1117 394 220 165 BbsI GAAGACnn'nnnn_ Cuts at: 0 1514 1896 Size: 1514 382 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1257 1291 1896 Size: 1257 34 605 Fragments arranged by size: 1257 605 34 Bme1580I G_kGCm'C Cuts at: 0 336 1896 Size: 336 1560 BmgBI CAC'GTC Cuts at: 0 1075 1896 Size: 1075 821 BmrI ACTGGGnnnn_n' Cuts at: 0 295 551 1553 1896 Size: 295 256 1002 343 Fragments arranged by size: 1002 343 295 256 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1205 1896 Size: 1205 691 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1000 1896 Size: 1000 896 BsaAI yAC'GTr Cuts at: 0 264 1896 Size: 264 1632 BsaBI GATnn'nnATC Cuts at: 0 703 1896 Size: 703 1193 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 895 997 1194 1355 1896 Size: 895 102 197 161 541 Fragments arranged by size: 895 541 197 161 102 BseYI C'CCAG_C Cuts at: 0 1521 1896 Size: 1521 375 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 446 1896 Size: 55 391 1450 Fragments arranged by size: 1450 391 55 BsiEI CG_ry'CG Cuts at: 0 660 1896 Size: 660 1236 BsmBI CGTCTCn'nnnn_ Cuts at: 0 647 742 1896 Size: 647 95 1154 Fragments arranged by size: 1154 647 95 Bsp1286I G_dGCh'C Cuts at: 0 336 1117 1337 1502 1896 Size: 336 781 220 165 394 Fragments arranged by size: 781 394 336 220 165 BspHI T'CATG_A Cuts at: 0 1221 1896 Size: 1221 675 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 77 1896 Size: 77 1819 BsrBI CCG'CTC Cuts at: 0 419 1896 Size: 419 1477 BsrDI GCAATG_nn' Cuts at: 0 717 1155 1896 Size: 717 438 741 Fragments arranged by size: 741 717 438 BsrFI r'CCGG_y Cuts at: 0 659 1135 1896 Size: 659 476 761 Fragments arranged by size: 761 659 476 BstBI TT'CG_AA Cuts at: 0 405 1896 Size: 405 1491 BstYI r'GATC_y Cuts at: 0 349 552 1896 Size: 349 203 1344 Fragments arranged by size: 1344 349 203 BtgI C'CryG_G Cuts at: 0 182 1600 1896 Size: 182 1418 296 Fragments arranged by size: 1418 296 182 BtsI GCAGTG_nn' Cuts at: 0 473 1439 1896 Size: 473 966 457 Fragments arranged by size: 966 473 457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1589 1624 1896 Size: 1589 35 272 Fragments arranged by size: 1589 272 35 DraI TTT'AAA Cuts at: 0 375 1896 Size: 375 1521 DraIII CAC_nnn'GTG Cuts at: 0 1561 1896 Size: 1561 335 EaeI y'GGCC_r Cuts at: 0 312 657 1896 Size: 312 345 1239 Fragments arranged by size: 1239 345 312 EagI C'GGCC_G Cuts at: 0 657 1896 Size: 657 1239 EarI CTCTTCn'nnn_ Cuts at: 0 127 1896 Size: 127 1769 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 503 1896 Size: 503 1393 EcoO109I rG'GnC_Cy Cuts at: 0 816 1896 Size: 816 1080 FspI TGC'GCA Cuts at: 0 907 1896 Size: 907 989 HaeII r_GCGC'y Cuts at: 0 10 141 1896 Size: 10 131 1755 Fragments arranged by size: 1755 131 10 HincII GTy'rAC Cuts at: 0 693 1896 Size: 693 1203 HindIII A'AGCT_T Cuts at: 0 1475 1896 Size: 1475 421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 645 1562 1896 Size: 645 917 334 Fragments arranged by size: 917 645 334 MspA1I CmG'CkG Cuts at: 0 211 1110 1157 1648 1896 Size: 211 899 47 491 248 Fragments arranged by size: 899 491 248 211 47 NaeI GCC'GGC Cuts at: 0 661 1137 1896 Size: 661 476 759 Fragments arranged by size: 759 661 476 NgoMIV G'CCGG_C Cuts at: 0 659 1135 1896 Size: 659 476 761 Fragments arranged by size: 761 659 476 NspI r_CATG'y Cuts at: 0 1819 1896 Size: 1819 77 PasI CC'CwG_GG Cuts at: 0 1165 1896 Size: 1165 731 PflMI CCAn_nnn'nTGG Cuts at: 0 1548 1565 1896 Size: 1548 17 331 Fragments arranged by size: 1548 331 17 PshAI GACnn'nnGTC Cuts at: 0 1414 1896 Size: 1414 482 PstI C_TGCA'G Cuts at: 0 210 1516 1896 Size: 210 1306 380 Fragments arranged by size: 1306 380 210 PvuII CAG'CTG Cuts at: 0 211 1157 1648 1896 Size: 211 946 491 248 Fragments arranged by size: 946 491 248 211 ScaI AGT'ACT Cuts at: 0 1469 1896 Size: 1469 427 SfcI C'TryA_G Cuts at: 0 206 828 1512 1896 Size: 206 622 684 384 Fragments arranged by size: 684 622 384 206 SmlI C'TyrA_G Cuts at: 0 979 1896 Size: 979 917 StyI C'CwwG_G Cuts at: 0 28 493 1896 Size: 28 465 1403 Fragments arranged by size: 1403 465 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1572 1896 Size: 1572 324 TatI w'GTAC_w Cuts at: 0 1467 1798 1896 Size: 1467 331 98 Fragments arranged by size: 1467 331 98 Tth111I GACn'n_nGTC Cuts at: 0 971 1896 Size: 971 925 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1545 1562 1896 Size: 1545 17 334 Fragments arranged by size: 1545 334 17 XmnI GAAnn'nnTTC Cuts at: 0 488 1896 Size: 488 1408 Enzymes that do cut: AcuI AflIII AloI AlwNI ApoI AvaI BanI BanII BbsI BcgI Bme1580I BmgBI BmrI BplI BpuEI BsaAI BsaBI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstBI BstYI BtgI BtsI CspCI DraI DraIII EaeI EagI EarI Eco57MI EcoO109I FspI HaeII HincII HindIII MmeI MspA1I NaeI NgoMIV NspI PasI PflMI PshAI PstI PvuII ScaI SfcI SmlI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmtI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BsiHKAI BsiWI BsmI BspEI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HpaI KasI KpnI MfeI MluI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XhoI XmaI ZraI