Restriction map for pFN21AE1553
 (Linear) (Six-base) MAPSORT of: KIEE1553.seq  Check: 3505  from: 1  to: 1896



KIEE1553.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1896 
   Size:        503    1393
 
AflIII A'CryG_T

Cuts at:      0     263    1074    1896 
   Size:        263     811     822

  Fragments arranged by size: 

                 822     811     263
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     776     808    1896 
   Size:        776      32    1088

  Fragments arranged by size: 

                1088     776      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     762    1565    1896 
   Size:        762     803     331

  Fragments arranged by size: 

                 803     762     331
 
ApoI r'AATT_y

Cuts at:      0     459    1896 
   Size:        459    1437
 
AvaI C'yCGr_G

Cuts at:      0     836     877    1896 
   Size:        836      41    1019

  Fragments arranged by size: 

                1019     836      41
 
BanI G'GyrC_C

Cuts at:      0     100    1896 
   Size:        100    1796
 
BanII G_rGCy'C

Cuts at:      0    1117    1337    1502    1896 
   Size:       1117     220     165     394

  Fragments arranged by size: 

                1117     394     220     165
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1514    1896 
   Size:       1514     382
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1257    1291    1896 
   Size:       1257      34     605

  Fragments arranged by size: 

                1257     605      34
 
Bme1580I G_kGCm'C

Cuts at:      0     336    1896 
   Size:        336    1560
 
BmgBI CAC'GTC

Cuts at:      0    1075    1896 
   Size:       1075     821
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     295     551    1553    1896 
   Size:        295     256    1002     343

  Fragments arranged by size: 

                1002     343     295     256
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1205    1896 
   Size:       1205     691
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1000    1896 
   Size:       1000     896
 
BsaAI yAC'GTr

Cuts at:      0     264    1896 
   Size:        264    1632
 
BsaBI GATnn'nnATC

Cuts at:      0     703    1896 
   Size:        703    1193
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     895     997    1194    1355    1896 
   Size:        895     102     197     161     541

  Fragments arranged by size: 

                 895     541     197     161     102
 
BseYI C'CCAG_C

Cuts at:      0    1521    1896 
   Size:       1521     375
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     446    1896 
   Size:         55     391    1450

  Fragments arranged by size: 

                1450     391      55
 
BsiEI CG_ry'CG

Cuts at:      0     660    1896 
   Size:        660    1236
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     647     742    1896 
   Size:        647      95    1154

  Fragments arranged by size: 

                1154     647      95
 
Bsp1286I G_dGCh'C

Cuts at:      0     336    1117    1337    1502    1896 
   Size:        336     781     220     165     394

  Fragments arranged by size: 

                 781     394     336     220     165
 
BspHI T'CATG_A

Cuts at:      0    1221    1896 
   Size:       1221     675
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      77    1896 
   Size:         77    1819
 
BsrBI CCG'CTC

Cuts at:      0     419    1896 
   Size:        419    1477
 
BsrDI GCAATG_nn'

Cuts at:      0     717    1155    1896 
   Size:        717     438     741

  Fragments arranged by size: 

                 741     717     438
 
BsrFI r'CCGG_y

Cuts at:      0     659    1135    1896 
   Size:        659     476     761

  Fragments arranged by size: 

                 761     659     476
 
BstBI TT'CG_AA

Cuts at:      0     405    1896 
   Size:        405    1491
 
BstYI r'GATC_y

Cuts at:      0     349     552    1896 
   Size:        349     203    1344

  Fragments arranged by size: 

                1344     349     203
 
BtgI C'CryG_G

Cuts at:      0     182    1600    1896 
   Size:        182    1418     296

  Fragments arranged by size: 

                1418     296     182
 
BtsI GCAGTG_nn'

Cuts at:      0     473    1439    1896 
   Size:        473     966     457

  Fragments arranged by size: 

                 966     473     457
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1589    1624    1896 
   Size:       1589      35     272

  Fragments arranged by size: 

                1589     272      35
 
DraI TTT'AAA

Cuts at:      0     375    1896 
   Size:        375    1521
 
DraIII CAC_nnn'GTG

Cuts at:      0    1561    1896 
   Size:       1561     335
 
EaeI y'GGCC_r

Cuts at:      0     312     657    1896 
   Size:        312     345    1239

  Fragments arranged by size: 

                1239     345     312
 
EagI C'GGCC_G

Cuts at:      0     657    1896 
   Size:        657    1239
 
EarI CTCTTCn'nnn_

Cuts at:      0     127    1896 
   Size:        127    1769
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1896 
   Size:        503    1393
 
EcoO109I rG'GnC_Cy

Cuts at:      0     816    1896 
   Size:        816    1080
 
FspI TGC'GCA

Cuts at:      0     907    1896 
   Size:        907     989
 
HaeII r_GCGC'y

Cuts at:      0      10     141    1896 
   Size:         10     131    1755

  Fragments arranged by size: 

                1755     131      10
 
HincII GTy'rAC

Cuts at:      0     693    1896 
   Size:        693    1203
 
HindIII A'AGCT_T

Cuts at:      0    1475    1896 
   Size:       1475     421
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     645    1562    1896 
   Size:        645     917     334

  Fragments arranged by size: 

                 917     645     334
 
MspA1I CmG'CkG

Cuts at:      0     211    1110    1157    1648    1896 
   Size:        211     899      47     491     248

  Fragments arranged by size: 

                 899     491     248     211      47
 
NaeI GCC'GGC

Cuts at:      0     661    1137    1896 
   Size:        661     476     759

  Fragments arranged by size: 

                 759     661     476
 
NgoMIV G'CCGG_C

Cuts at:      0     659    1135    1896 
   Size:        659     476     761

  Fragments arranged by size: 

                 761     659     476
 
NspI r_CATG'y

Cuts at:      0    1819    1896 
   Size:       1819      77
 
PasI CC'CwG_GG

Cuts at:      0    1165    1896 
   Size:       1165     731
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1548    1565    1896 
   Size:       1548      17     331

  Fragments arranged by size: 

                1548     331      17
 
PshAI GACnn'nnGTC

Cuts at:      0    1414    1896 
   Size:       1414     482
 
PstI C_TGCA'G

Cuts at:      0     210    1516    1896 
   Size:        210    1306     380

  Fragments arranged by size: 

                1306     380     210
 
PvuII CAG'CTG

Cuts at:      0     211    1157    1648    1896 
   Size:        211     946     491     248

  Fragments arranged by size: 

                 946     491     248     211
 
ScaI AGT'ACT

Cuts at:      0    1469    1896 
   Size:       1469     427
 
SfcI C'TryA_G

Cuts at:      0     206     828    1512    1896 
   Size:        206     622     684     384

  Fragments arranged by size: 

                 684     622     384     206
 
SmlI C'TyrA_G

Cuts at:      0     979    1896 
   Size:        979     917
 
StyI C'CwwG_G

Cuts at:      0      28     493    1896 
   Size:         28     465    1403

  Fragments arranged by size: 

                1403     465      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1572    1896 
   Size:       1572     324
 
TatI w'GTAC_w

Cuts at:      0    1467    1798    1896 
   Size:       1467     331      98

  Fragments arranged by size: 

                1467     331      98
 
Tth111I GACn'n_nGTC

Cuts at:      0     971    1896 
   Size:        971     925
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1545    1562    1896 
   Size:       1545      17     334

  Fragments arranged by size: 

                1545     334      17
 
XmnI GAAnn'nnTTC

Cuts at:      0     488    1896 
   Size:        488    1408
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI     ApoI     AvaI     BanI    BanII
     BbsI     BcgI Bme1580I    BmgBI     BmrI     BplI    BpuEI    BsaAI
    BsaBI    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BstBI    BstYI     BtgI     BtsI
    CspCI     DraI   DraIII     EaeI     EagI     EarI  Eco57MI EcoO109I
     FspI    HaeII   HincII  HindIII     MmeI   MspA1I     NaeI   NgoMIV
     NspI     PasI    PflMI    PshAI     PstI    PvuII     ScaI     SfcI
     SmlI     StyI    TaqII     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmtI     BpmI   Bpu10I     BsaI    BsaHI
    BsaWI    BsaXI  BsiHKAI    BsiWI     BsmI    BspEI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
    FspAI    Hin4I     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI     XbaI     XhoI     XmaI     ZraI
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