################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI DpnII DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 325 GTCTAC GT^[AC][GT]AC AhdI 138 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 92 GGGCCC GGGCC^C AspEI 138 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 40 GGCACC G^G[CT][GA]CC BcgI 314 GCATCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 465 GTATCC GTATCC(6/5) BsgI 490 CTGCAC GTGCAG(16/14) BsmBI 16 CGTCTC CGTCTC(1/5) BsmFI 301 GTCCC GGGAC(10/14) BspMI 186 ACCTGC ACCTGC(4/8) BstXI 549 CCACACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 215 GCAAGC GC[ACGT]^[ACGT]GC CpoI 248 CGGACCG CG^G[AT]CCG DraII 91 GGGGCCC [GA]G^G[ACGT]CC[CT] DsaI 504 CCACGG C^C[GA][CT]GG Eam1105I 138 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 91 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoT22I 160 ATGCAT ATGCA^T MaeII 15 ACGT A^CGT MslI 550 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 128 CAGCTG C[AC]G^C[GT]G NsiI 160 ATGCAT ATGCA^T PflMI 453 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 475 CTGCAG CTGCA^G PvuII 128 CAGCTG CAG^CTG RsrII 248 CGGACCG CG^G[AT]CCG SapI 408 GCTCTTC GCTCTTC(1/4) ScaI 586 AGTACT AGT^ACT SfcI 475 CTGCAG C^T[GA][CT]AG SmlI 279 CTTGAG C^T[CT][GA]AG TaqI 496 TCGA T^CGA Tsp509I 289 AATT ^AATT TthHB8I 496 TCGA T^CGA Van91I 453 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 96 CCAGGGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BanII 92 GGGCCC G[GA]GC[CT]^C 100 GGGCTC BbsI 340 GTCTTC GAAGAC(2/6) 592 GTCTTC BbvCI 444 CCTCAGC CCTCAGC(-5/-2) 576 CCTCAGC BfaI 571 CTAG C^TAG 584 CTAG BmyI 92 GGGCCC G[AGT]GC[ACT]^C 100 GGGCTC BpmI 262 CTCCAG CTGGAG(16/14) 234 CTGGAG BpuAI 340 GTCTTC GAAGAC(2/6) 592 GTCTTC BsaI 206 GAGACC GGTCTC(1/5) 533 GAGACC Bsp1286I 92 GGGCCC G[AGT]GC[ACT]^C 100 GGGCTC BsrI 308 CCAGT ACTGG(1/-1) 420 CCAGT BstF5I 561 CATCC GGATG(2/0) 267 GGATG DdeI 445 CTCAG C^T[ACGT]AG 577 CTCAG EarI 19 CTCTTC CTCTTC(1/4) 409 CTCTTC EcoT14I 28 CCAAGG C^C[AT][AT]GG 365 CCAAGG FokI 561 CATCC GGATG(9/13) 267 GGATG HphI 195 TCACC GGTGA(8/7) 524 TCACC Ksp632I 19 CTCTTC CTCTTC(1/4) 409 CTCTTC MaeI 571 CTAG C^TAG 584 CTAG MwoI 75 GCTGGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 133 GCCCAGACTGC NlaIII 397 CATG CATG^ 433 CATG StyI 28 CCAAGG C^C[AT][AT]GG 365 CCAAGG TspRI 227 CACTG CA[GC]TG[ACGT][ACGT]^ 309 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AfaI AluI AvaII BbvI BsaJI BseRI BsiYI BslI BsmAI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII ItaI MboII MnlI MvaI NlaIV RsaI Sau96I ScrFI SfaNI TseI