(Linear) (Six-base) MAPSORT of: KIEE1386.seq Check: 2196 from: 1 to: 615 KIEE1386.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AccI GT'mk_AC Cuts at: 0 326 615 Size: 326 289 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 615 Size: 110 505 AhdI GACnn_n'nnGTC Cuts at: 0 143 615 Size: 143 472 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 5 37 615 Size: 5 32 578 Fragments arranged by size: 578 32 5 ApaI G_GGCC'C Cuts at: 0 96 615 Size: 96 519 BanI G'GyrC_C Cuts at: 0 40 615 Size: 40 575 BanII G_rGCy'C Cuts at: 0 96 104 615 Size: 96 8 511 Fragments arranged by size: 511 96 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 333 585 615 Size: 333 252 30 Fragments arranged by size: 333 252 30 BbvCI CC'TCA_GC Cuts at: 0 445 577 615 Size: 445 132 38 Fragments arranged by size: 445 132 38 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 303 337 615 Size: 303 34 278 Fragments arranged by size: 303 278 34 BciVI GTATCCnnnnn_n' Cuts at: 0 476 615 Size: 476 139 BfrBI ATG'CAT Cuts at: 0 162 615 Size: 162 453 Bme1580I G_kGCm'C Cuts at: 0 96 615 Size: 96 519 BmrI ACTGGGnnnn_n' Cuts at: 0 414 615 Size: 414 201 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 255 615 Size: 247 8 360 Fragments arranged by size: 360 247 8 Bpu10I CC'TnA_GC Cuts at: 0 445 577 615 Size: 445 132 38 Fragments arranged by size: 445 132 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 615 Size: 300 315 BsaI GGTCTCn'nnnn_ Cuts at: 0 200 527 615 Size: 200 327 88 Fragments arranged by size: 327 200 88 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 41 71 615 Size: 41 30 544 Fragments arranged by size: 544 41 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 366 405 433 615 Size: 46 320 39 28 182 Fragments arranged by size: 320 182 46 39 28 BseYI C'CCAG_C Cuts at: 0 211 615 Size: 211 404 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 615 Size: 475 140 BsmBI CGTCTCn'nnnn_ Cuts at: 0 22 615 Size: 22 593 Bsp1286I G_dGCh'C Cuts at: 0 96 104 615 Size: 96 8 511 Fragments arranged by size: 511 96 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 195 615 Size: 195 420 BstXI CCAn_nnnn'nTGG Cuts at: 0 556 615 Size: 556 59 BtgI C'CryG_G Cuts at: 0 504 615 Size: 504 111 BtsI GCAGTG_nn' Cuts at: 0 226 615 Size: 226 389 EarI CTCTTCn'nnn_ Cuts at: 0 25 415 615 Size: 25 390 200 Fragments arranged by size: 390 200 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 247 255 615 Size: 110 137 8 360 Fragments arranged by size: 360 137 110 8 EcoO109I rG'GnC_Cy Cuts at: 0 92 615 Size: 92 523 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 129 161 259 291 450 482 526 558 Size: 129 32 98 32 159 32 44 32 Cuts at: 558 615 Size: 57 Fragments arranged by size: 159 129 98 57 44 32 32 32 32 MslI CAynn'nnrTG Cuts at: 0 554 615 Size: 554 61 MspA1I CmG'CkG Cuts at: 0 130 615 Size: 130 485 NsiI A_TGCA'T Cuts at: 0 164 615 Size: 164 451 PasI CC'CwG_GG Cuts at: 0 96 528 615 Size: 96 432 87 Fragments arranged by size: 432 96 87 PflMI CCAn_nnn'nTGG Cuts at: 0 459 615 Size: 459 156 PfoI T'CCnGG_A Cuts at: 0 263 615 Size: 263 352 PspOMI G'GGCC_C Cuts at: 0 92 615 Size: 92 523 PstI C_TGCA'G Cuts at: 0 479 615 Size: 479 136 PvuII CAG'CTG Cuts at: 0 130 615 Size: 130 485 RsrII CG'GwC_CG Cuts at: 0 249 615 Size: 249 366 SapI GCTCTTCn'nnn_ Cuts at: 0 415 615 Size: 415 200 ScaI AGT'ACT Cuts at: 0 588 615 Size: 588 27 SfcI C'TryA_G Cuts at: 0 475 615 Size: 475 140 SmlI C'TyrA_G Cuts at: 0 279 615 Size: 279 336 StyI C'CwwG_G Cuts at: 0 28 365 615 Size: 28 337 250 Fragments arranged by size: 337 250 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 541 562 615 Size: 541 21 53 Fragments arranged by size: 541 53 21 TatI w'GTAC_w Cuts at: 0 586 615 Size: 586 29 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 103 615 Size: 103 512 Enzymes that do cut: AccI AcuI AhdI AloI ApaI BanI BanII BbsI BbvCI BcgI BciVI BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspMI BstXI BtgI BtsI EarI Eco57MI EcoO109I Hin4I MslI MspA1I NsiI PasI PflMI PfoI PspOMI PstI PvuII RsrII SapI ScaI SfcI SmlI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BclI BglI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI