(Circular) (Six-base) MAPSORT of: pFN21AB5863.seq Check: 2523 from: 1 to: 6088 pFN21AB5863 6088 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2671 2671 Size: 6088 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 3431 1937 Size: 328 1166 4594 Fragments arranged by size: 4594 1166 328 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2341 4151 5535 5803 1632 Size: 709 1810 1384 268 1917 Fragments arranged by size: 1917 1810 1384 709 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2113 2379 2676 3831 5403 2113 Size: 266 297 1155 1572 2798 Fragments arranged by size: 2798 1572 1155 297 266 ApaI G_GGCC'C Cuts at: 3219 3219 Size: 6088 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2049 2217 3397 3623 3775 5884 1493 Size: 556 168 1180 226 152 2109 1697 Fragments arranged by size: 2109 1697 1180 556 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3176 3413 1870 Size: 73 1233 237 4545 Fragments arranged by size: 4545 1233 237 73 BamHI G'GATC_C Cuts at: 2118 3336 3418 3785 2118 Size: 1218 82 367 4421 Fragments arranged by size: 4421 1218 367 82 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3239 3382 3409 4864 618 Size: 325 217 627 1452 143 27 1455 1842 Fragments arranged by size: 1842 1455 1452 627 325 217 143 27 BanII G_rGCy'C Cuts at: 1391 2154 2994 3219 3288 3407 1391 Size: 763 840 225 69 119 4072 Fragments arranged by size: 4072 840 763 225 119 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2616 2625 2851 928 Size: 971 717 9 226 4165 Fragments arranged by size: 4165 971 717 226 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2320 2354 4279 4313 1903 Size: 34 383 34 1925 34 3678 Fragments arranged by size: 3678 1925 383 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2376 2720 4699 Size: 107 122 71 631 605 704 344 1979 Cuts at: 4699 136 Size: 1525 Fragments arranged by size: 1979 1525 704 631 605 344 122 107 71 BglII A'GATC_T Cuts at: 2190 6083 2190 Size: 3893 2195 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2104 2328 2955 3219 3348 3385 4149 5305 Size: 314 224 627 264 129 37 764 1156 Cuts at: 5305 1790 Size: 2573 Fragments arranged by size: 2573 1156 764 627 314 264 224 129 37 BmgBI CAC'GTC Cuts at: 1318 2263 1318 Size: 945 5143 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2165 2189 4748 1239 Size: 832 94 24 2559 2579 Fragments arranged by size: 2579 2559 832 94 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3005 4751 882 Size: 617 1506 1746 2219 Fragments arranged by size: 2219 1746 1506 617 BsaAI yAC'GTr Cuts at: 493 1204 2334 3879 493 Size: 711 1130 1545 2702 Fragments arranged by size: 2702 1545 1130 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2050 2080 2162 2192 2288 Size: 30 165 30 647 30 82 30 96 Cuts at: 2288 2318 1178 Size: 30 4948 Fragments arranged by size: 4948 647 165 96 82 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2058 2162 2201 2219 2477 1186 Size: 872 104 39 18 258 4797 Fragments arranged by size: 4797 872 258 104 39 18 BseYI C'CCAG_C Cuts at: 2379 2506 2820 2922 3027 3267 3378 5291 5787 Size: 127 314 102 105 240 111 1913 496 Cuts at: 5787 2379 Size: 2680 Fragments arranged by size: 2680 1913 496 314 240 127 111 105 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2428 2897 3142 1163 Size: 573 692 469 245 4109 Fragments arranged by size: 4109 692 573 469 245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 2301 2730 2888 2994 4149 4234 5305 2154 Size: 147 429 158 106 1155 85 1071 2937 Fragments arranged by size: 2937 1155 1071 429 158 147 106 85 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2154 2301 2328 2730 2888 2955 Size: 399 314 50 147 27 402 158 67 Cuts at: 2955 2994 3219 3288 3348 3385 3407 4149 4234 Size: 39 225 69 60 37 22 742 85 Cuts at: 4234 5305 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 314 225 158 147 85 69 67 60 50 39 37 27 22 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3132 3977 5707 3132 Size: 845 1730 3513 Fragments arranged by size: 3513 1730 845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2671 2820 3443 844 Size: 1827 149 623 3489 Fragments arranged by size: 3489 1827 623 149 BsrBI CCG'CTC Cuts at: 1394 1823 2173 3975 1394 Size: 429 350 1802 3507 Fragments arranged by size: 3507 1802 429 350 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3002 3183 4732 4919 1700 Size: 110 1192 181 1549 187 2869 Fragments arranged by size: 2869 1549 1192 187 181 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 3017 4148 5160 3017 Size: 1131 1012 3945 Fragments arranged by size: 3945 1131 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 2270 1184 Size: 1086 5002 BstYI r'GATC_y Cuts at: 1972 2118 2190 2629 3336 3418 3785 4170 4187 Size: 146 72 439 707 82 367 385 17 Cuts at: 4187 5639 5725 5737 6083 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 707 439 385 367 346 146 86 82 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2633 3102 3219 3235 3340 513 Size: 552 1568 469 117 16 105 3261 Fragments arranged by size: 3261 1568 552 469 117 105 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2887 487 Size: 683 56 1661 3688 Fragments arranged by size: 3688 1661 683 56 BtsI GCAGTG_nn' Cuts at: 2531 2544 2730 2761 2906 3604 4398 4418 2531 Size: 13 186 31 145 698 794 20 4201 Fragments arranged by size: 4201 794 698 186 145 31 20 13 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DraIII CAC_nnn'GTG Cuts at: 2110 2110 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 3004 3535 Size: 54 1425 50 275 18 207 967 531 Cuts at: 3535 4424 6053 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 967 889 531 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3368 4018 1353 Size: 2015 650 3423 Fragments arranged by size: 3423 2015 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 2992 2152 Size: 840 5248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2165 2189 2341 4151 4748 5535 Size: 393 439 94 24 152 1810 597 787 Cuts at: 5535 5803 1239 Size: 268 1524 Fragments arranged by size: 1810 1524 787 597 439 393 268 152 94 24 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 6088 EcoO109I rG'GnC_Cy Cuts at: 2559 3215 2559 Size: 656 5432 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2875 2875 Size: 6088 FseI GG_CCGG'CC Cuts at: 3006 3006 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2482 2821 5235 1678 Size: 804 339 2414 2531 Fragments arranged by size: 2531 2414 804 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4729 4761 4803 4835 1373 Size: 32 135 32 3157 32 42 32 2626 Fragments arranged by size: 3157 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 3432 3678 669 Size: 988 281 328 1166 246 3079 Fragments arranged by size: 3079 1166 988 328 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 2419 3687 2419 Size: 1268 4820 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 10 Size: 54 1425 343 207 4059 Fragments arranged by size: 4059 1425 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 4046 4405 4564 518 Size: 648 2880 359 159 2042 Fragments arranged by size: 2880 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2361 2376 2635 2756 2973 3504 4181 5329 Size: 755 15 259 121 217 531 677 1148 Cuts at: 5329 5574 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 755 677 531 259 245 217 121 15 NaeI GCC'GGC Cuts at: 3004 3004 Size: 6088 NcoI C'CATG_G Cuts at: 513 1065 3219 3340 513 Size: 552 2154 121 3261 Fragments arranged by size: 3261 2154 552 121 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 3002 3002 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 3160 3446 4991 1132 Size: 97 1931 286 1545 2229 Fragments arranged by size: 2229 1931 1545 286 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2230 2281 2767 1108 Size: 1122 51 486 4429 Fragments arranged by size: 4429 1122 486 51 PfoI T'CCnGG_A Cuts at: 2142 2964 2142 Size: 822 5266 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PmlI CAC'GTG Cuts at: 2334 2334 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 3215 3215 Size: 6088 PstI C_TGCA'G Cuts at: 830 2526 3440 830 Size: 1696 914 3478 Fragments arranged by size: 3478 1696 914 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 2361 2361 Size: 6088 SacI G_AGCT'C Cuts at: 2154 2994 2154 Size: 840 5248 SacII CC_GC'GG Cuts at: 2636 2636 Size: 6088 SalI G'TCGA_C Cuts at: 1936 2264 3430 1936 Size: 328 1166 4594 Fragments arranged by size: 4594 1166 328 SapI GCTCTTCn'nnn_ Cuts at: 3368 3368 Size: 6088 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SexAI A'CCwGG_T Cuts at: 2106 3242 2106 Size: 1136 4952 SfcI C'TryA_G Cuts at: 826 1045 2482 2522 2875 3436 4571 5252 5443 Size: 219 1437 40 353 561 1135 681 191 Cuts at: 5443 826 Size: 1471 Fragments arranged by size: 1471 1437 1135 681 561 353 219 191 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4192 5093 5355 820 Size: 197 926 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2211 2292 3219 3340 3527 513 Size: 552 1146 81 927 121 187 3074 Fragments arranged by size: 3074 1146 927 552 187 121 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2734 4115 4132 4285 4470 4915 325 Size: 1255 1154 1381 17 153 185 445 1498 Fragments arranged by size: 1498 1381 1255 1154 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2050 2082 2162 2194 2288 2320 6048 6080 2050 Size: 32 80 32 94 32 3728 32 2058 Fragments arranged by size: 3728 2058 94 80 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2140 2140 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI