(Circular) (Six-base) MAPSORT of: pFN21AE1537.seq Check: 7587 from: 1 to: 7441 pFN21AE1537 7441 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2146 2146 Size: 7441 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4397 5251 278 Size: 53 83 186 936 2861 854 2468 Fragments arranged by size: 2861 2468 936 854 186 83 53 AccI GT'mk_AC Cuts at: 1937 4784 1937 Size: 2847 4594 Acc65I G'GTAC_C Cuts at: 4762 4762 Size: 7441 AclI AA'CG_TT Cuts at: 1563 5568 5941 1563 Size: 4005 373 3063 Fragments arranged by size: 4005 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3686 4526 5504 6888 7156 1632 Size: 2054 840 978 1384 268 1917 Fragments arranged by size: 2054 1917 1384 978 840 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7244 AflIII A'CryG_T Cuts at: 1203 6340 7317 1203 Size: 5137 977 1327 Fragments arranged by size: 5137 1327 977 AgeI A'CCGG_T Cuts at: 6272 6272 Size: 7441 AhdI GACnn_n'nnGTC Cuts at: 2719 6170 2719 Size: 3451 3990 AleI CACnn'nnGTG Cuts at: 1166 3967 1166 Size: 2801 4640 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3799 4127 1184 Size: 2615 328 4498 Fragments arranged by size: 4498 2615 328 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3593 3625 3593 Size: 32 7409 AlwNI CAG_nnn'CTG Cuts at: 2149 2518 3023 3697 4516 5184 6756 2149 Size: 369 505 674 819 668 1572 2834 Fragments arranged by size: 2834 1572 819 674 668 505 369 ApaI G_GGCC'C Cuts at: 2270 3065 3962 2270 Size: 795 897 5749 Fragments arranged by size: 5749 897 795 ApaLI G'TGCA_C Cuts at: 5498 6654 5498 Size: 1156 6285 ApoI r'AATT_y Cuts at: 1493 3795 4673 4750 4976 5128 7237 1493 Size: 2302 878 77 226 152 2109 1697 Fragments arranged by size: 2302 2109 1697 878 226 152 77 AseI AT'TA_AT Cuts at: 160 1032 5995 160 Size: 872 4963 1606 Fragments arranged by size: 4963 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2568 2931 3128 4333 4344 4460 Size: 73 76 549 363 197 1205 11 116 Cuts at: 4460 4554 4766 1870 Size: 94 212 4545 Fragments arranged by size: 4545 1205 549 363 212 197 116 94 76 73 11 BamHI G'GATC_C Cuts at: 4283 4771 5138 4283 Size: 488 367 6586 Fragments arranged by size: 6586 488 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 2338 3085 3648 3864 Size: 325 217 627 379 172 747 563 216 Cuts at: 3864 4762 6217 618 Size: 898 1455 1842 Fragments arranged by size: 1842 1455 898 747 627 563 379 325 217 216 172 BanII G_rGCy'C Cuts at: 1391 2261 2270 2841 2953 3065 3128 3405 3521 Size: 870 9 571 112 112 63 277 116 Cuts at: 3521 3750 3962 4595 4760 1391 Size: 229 212 633 165 4072 Fragments arranged by size: 4072 870 633 571 277 229 212 165 116 112 112 63 9 BbeI G_GCGC'C Cuts at: 2170 3089 3868 2170 Size: 919 779 5743 Fragments arranged by size: 5743 919 779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2016 2327 4539 928 Size: 971 117 311 2212 3830 Fragments arranged by size: 3830 2212 971 311 117 BbvCI CC'TCA_GC Cuts at: 2082 3513 3842 4358 2082 Size: 1431 329 516 5165 Fragments arranged by size: 5165 1431 516 329 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5632 5666 1903 Size: 34 3695 34 3678 Fragments arranged by size: 3695 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5333 6543 5333 Size: 1210 6231 BclI T'GATC_A Cuts at: 1548 1548 Size: 7441 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2024 2285 4464 Size: 107 122 71 631 605 352 261 2179 Cuts at: 4464 6052 136 Size: 1588 1525 Fragments arranged by size: 2179 1588 1525 631 605 352 261 122 107 71 BglII A'GATC_T Cuts at: 7436 7436 Size: 7441 BlpI GC'TnA_GC Cuts at: 2798 3908 4628 4858 2798 Size: 1110 720 230 5381 Fragments arranged by size: 5381 1110 720 230 Bme1580I G_kGCm'C Cuts at: 1790 2270 3065 3962 5502 6658 1790 Size: 480 795 897 1540 1156 2573 Fragments arranged by size: 2573 1540 1156 897 795 480 BmgBI CAC'GTC Cuts at: 1318 3487 3808 3969 1318 Size: 2169 321 161 4790 Fragments arranged by size: 4790 2169 321 161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2273 5172 6130 Size: 416 53 83 399 24 856 2899 958 Cuts at: 6130 442 Size: 1753 Fragments arranged by size: 2899 1753 958 856 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7441 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 4370 2134 Size: 2236 5205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2171 2336 2486 2549 2627 2991 3056 Size: 890 42 165 150 63 78 364 65 Cuts at: 3056 3095 3156 3188 4022 4383 4676 6101 1239 Size: 39 61 32 834 361 293 1425 2579 Fragments arranged by size: 2579 1425 890 834 364 361 293 165 150 78 65 63 61 42 39 32 Bpu10I CC'TnA_GC Cuts at: 2082 3513 3842 4317 4358 4589 2082 Size: 1431 329 475 41 231 4934 Fragments arranged by size: 4934 1431 475 329 231 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3833 4049 5566 6431 6729 3833 Size: 216 1517 865 298 4545 Fragments arranged by size: 4545 1517 865 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 4681 6104 882 Size: 617 3135 47 1423 2219 Fragments arranged by size: 3135 2219 1423 617 47 BsaAI yAC'GTr Cuts at: 493 1204 2200 2581 3298 5232 493 Size: 711 996 381 717 1934 2702 Fragments arranged by size: 2702 1934 996 717 711 381 BsaBI GATnn'nnATC Cuts at: 7435 7435 Size: 7441 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2167 2501 3027 3086 Size: 53 83 186 936 634 334 526 59 Cuts at: 3086 3865 4088 4394 5248 5630 6265 275 Size: 779 223 306 854 382 635 1451 Fragments arranged by size: 1451 936 854 779 635 634 526 382 334 306 223 186 83 59 53 BsaWI w'CCGG_w Cuts at: 2739 2904 2937 5874 6272 6546 6693 7104 2739 Size: 165 33 2937 398 274 147 411 3076 Fragments arranged by size: 3076 2937 411 398 274 165 147 33 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2378 2408 1178 Size: 30 165 30 975 30 6211 Fragments arranged by size: 6211 975 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2624 3383 3488 3529 4346 4542 4652 4673 Size: 1438 759 105 41 817 196 110 21 Cuts at: 4673 4682 1186 Size: 9 3945 Fragments arranged by size: 3945 1438 817 759 196 110 105 41 21 9 BseYI C'CCAG_C Cuts at: 2170 2254 2523 3040 3389 4328 4594 4725 6644 Size: 84 269 517 349 939 266 131 1919 Cuts at: 6644 7140 2170 Size: 496 2471 Fragments arranged by size: 2471 1919 939 517 496 349 269 266 131 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 2966 3209 3302 3644 4720 1163 Size: 573 705 525 243 93 342 1076 3884 Fragments arranged by size: 3884 1076 705 573 525 342 243 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2768 3161 3433 4891 5652 6680 Size: 275 186 767 393 272 1458 761 1028 Cuts at: 6680 1540 Size: 2301 Fragments arranged by size: 2301 1458 1028 767 761 393 275 272 186 BsiHKAI G_wGCw'C Cuts at: 3405 4264 5502 5587 6658 3405 Size: 859 1238 85 1071 4188 Fragments arranged by size: 4188 1238 1071 859 85 BsmI GAATG_Cn' Cuts at: 3353 3415 4952 5045 3353 Size: 62 1537 93 5749 Fragments arranged by size: 5749 1537 93 62 BsmBI CGTCTCn'nnnn_ Cuts at: 3477 4385 4401 3477 Size: 908 16 6517 Fragments arranged by size: 6517 908 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2270 2841 2953 3065 3128 3405 Size: 399 471 9 571 112 112 63 277 Cuts at: 3405 3521 3750 3962 4264 4595 4760 5502 5587 Size: 116 229 212 302 331 165 742 85 Cuts at: 5587 6658 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 571 471 399 331 302 277 229 212 165 116 112 112 85 63 9 BspEI T'CCGG_A Cuts at: 2739 7104 2739 Size: 4365 3076 BspHI T'CATG_A Cuts at: 5330 7060 5330 Size: 1730 5711 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2146 2434 3846 4032 4632 4796 844 Size: 1302 288 1412 186 600 164 3489 Fragments arranged by size: 3489 1412 1302 600 288 186 164 BsrBI CCG'CTC Cuts at: 1394 1823 2575 2590 4054 4246 5328 1394 Size: 429 752 15 1464 192 1082 3507 Fragments arranged by size: 3507 1464 1082 752 429 192 15 BsrDI GCAATG_nn' Cuts at: 65 1248 4384 5936 6110 65 Size: 1183 3136 1552 174 1396 Fragments arranged by size: 3136 1552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2341 2457 2760 6085 6272 1700 Size: 110 531 116 303 3325 187 2869 Fragments arranged by size: 3325 2869 531 303 187 116 110 BsrGI T'GTAC_A Cuts at: 96 2676 96 Size: 2580 4861 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7441 BssSI C'ACGA_G Cuts at: 3953 5501 6513 3953 Size: 1548 1012 4881 Fragments arranged by size: 4881 1548 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3232 4009 794 Size: 2438 777 4226 Fragments arranged by size: 4226 2438 777 BstBI TT'CG_AA Cuts at: 6245 6245 Size: 7441 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7441 BstXI CCAn_nnnn'nTGG Cuts at: 3969 3969 Size: 7441 BstYI r'GATC_y Cuts at: 1972 4283 4771 5138 5523 5540 6992 7078 7090 Size: 2311 488 367 385 17 1452 86 12 Cuts at: 7090 7436 1972 Size: 346 1977 Fragments arranged by size: 2311 1977 1452 488 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2451 2655 3339 3644 3681 4479 Size: 552 939 447 204 684 305 37 798 Cuts at: 4479 513 Size: 3475 Fragments arranged by size: 3475 939 798 684 552 447 305 204 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6702 Fragments arranged by size: 6702 683 56 BtsI GCAGTG_nn' Cuts at: 3999 4957 5751 5771 3999 Size: 958 794 20 5669 Fragments arranged by size: 5669 958 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7441 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7406 DraI TTT'AAA Cuts at: 4745 4745 Size: 7441 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6448 809 Size: 507 5132 1802 Fragments arranged by size: 5132 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4158 4384 4888 Size: 54 1425 50 275 18 2328 226 504 Cuts at: 4888 5777 7406 8 Size: 889 1629 43 Fragments arranged by size: 2328 1629 1425 889 504 275 226 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4888 1537 Size: 275 3076 4090 Fragments arranged by size: 4090 3076 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 3177 3332 3738 5371 1353 Size: 765 1059 155 406 1633 3423 Fragments arranged by size: 3423 1633 1059 765 406 155 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2600 4129 4541 6028 6402 6548 Size: 1064 138 1011 1529 412 1487 374 146 Cuts at: 6548 387 Size: 1280 Fragments arranged by size: 1529 1487 1280 1064 1011 412 374 146 138 EcoICRI GAG'CTC Cuts at: 3403 3403 Size: 7441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2171 2336 2486 2549 2627 2991 Size: 393 497 42 165 150 63 78 364 Cuts at: 2991 3056 3095 3156 3188 3686 4022 4383 4526 Size: 65 39 61 32 498 336 361 143 Cuts at: 4526 4676 5504 6101 6888 7156 1239 Size: 150 828 597 787 268 1524 Fragments arranged by size: 1524 828 787 597 498 497 393 364 361 336 268 165 150 150 143 78 65 63 61 42 39 32 EcoNI CCTnn'n_nnAGG Cuts at: 2153 3470 2153 Size: 1317 6124 EcoO109I rG'GnC_Cy Cuts at: 2039 2222 2267 3061 3601 3835 3958 4457 4547 Size: 183 45 794 540 234 123 499 90 Cuts at: 4547 4570 2039 Size: 23 4910 Fragments arranged by size: 4910 794 540 499 234 183 123 90 45 23 EcoRI G'AATT_C Cuts at: 4750 5128 4750 Size: 378 7063 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2305 2305 Size: 7441 FspI TGC'GCA Cuts at: 3302 5947 3302 Size: 2645 4796 FspAI rTGC'GCAy Cuts at: 3302 3302 Size: 7441 HaeII r_GCGC'y Cuts at: 1678 2170 3089 3868 4045 6588 1678 Size: 492 919 779 177 2543 2531 Fragments arranged by size: 2543 2531 919 779 492 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 2705 2737 2914 Size: 32 135 32 806 32 295 32 177 Cuts at: 2914 2946 6082 6114 6156 6188 1373 Size: 32 3136 32 42 32 2626 Fragments arranged by size: 3136 2626 806 295 177 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2662 3811 4785 5031 669 Size: 988 281 724 1149 974 246 3079 Fragments arranged by size: 3079 1149 988 974 724 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7441 HpaI GTT'AAC Cuts at: 5031 5031 Size: 7441 KasI G'GCGC_C Cuts at: 2166 3085 3864 2166 Size: 919 779 5743 Fragments arranged by size: 5743 919 779 KpnI G_GTAC'C Cuts at: 4766 4766 Size: 7441 MfeI C'AATT_G Cuts at: 5040 5040 Size: 7441 MluI A'CGCG_T Cuts at: 7317 7317 Size: 7441 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2748 4221 4644 6555 6739 1305 Size: 1443 1473 423 1911 184 2007 Fragments arranged by size: 2007 1911 1473 1443 423 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4160 10 Size: 54 1425 343 2328 3291 Fragments arranged by size: 3291 2328 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3410 3686 3967 5399 5758 5917 Size: 648 1329 915 276 281 1432 359 159 Cuts at: 5917 518 Size: 2042 Fragments arranged by size: 2042 1432 1329 915 648 359 281 276 159 MspA1I CmG'CkG Cuts at: 1606 2327 2410 2464 2527 2823 3646 3664 3694 Size: 721 83 54 63 296 823 18 30 Cuts at: 3694 3862 4403 4654 4857 5534 6682 6927 1606 Size: 168 541 251 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 721 677 541 296 251 245 203 168 83 63 54 30 18 NaeI GCC'GGC Cuts at: 2459 2459 Size: 7441 NarI GG'CG_CC Cuts at: 2167 3086 3865 2167 Size: 919 779 5743 Fragments arranged by size: 5743 919 779 NcoI C'CATG_G Cuts at: 513 1065 2004 3339 513 Size: 552 939 1335 4615 Fragments arranged by size: 4615 1335 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7441 NgoMIV G'CCGG_C Cuts at: 2457 2457 Size: 7441 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7441 NotI GC'GGCC_GC Cuts at: 4888 4888 Size: 7441 NspI r_CATG'y Cuts at: 1132 1229 2246 2254 2683 3307 4007 4799 6344 Size: 97 1017 8 429 624 700 792 1545 Cuts at: 6344 1132 Size: 2229 Fragments arranged by size: 2229 1545 1017 792 700 624 429 97 8 PasI CC'CwG_GG Cuts at: 1348 2505 4574 1348 Size: 1157 2069 4215 Fragments arranged by size: 4215 2069 1157 PciI A'CATG_T Cuts at: 6340 6340 Size: 7441 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 2182 3340 1108 Size: 1041 33 1158 5209 Fragments arranged by size: 5209 1158 1041 33 PfoI T'CCnGG_A Cuts at: 2292 2313 2808 4614 2292 Size: 21 495 1806 5119 Fragments arranged by size: 5119 1806 495 21 PmeI GTTT'AAAC Cuts at: 4745 4745 Size: 7441 PmlI CAC'GTG Cuts at: 2200 2581 3298 2200 Size: 381 717 6343 Fragments arranged by size: 6343 717 381 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4341 4373 5510 5542 7023 7055 4341 Size: 32 1137 32 1481 32 4727 Fragments arranged by size: 4727 1481 1137 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2222 3601 3835 4570 2222 Size: 1379 234 735 5093 Fragments arranged by size: 5093 1379 735 234 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7441 PsiI TTA'TAA Cuts at: 5011 5011 Size: 7441 PspOMI G'GGCC_C Cuts at: 2266 3061 3958 2266 Size: 795 897 5749 Fragments arranged by size: 5749 897 795 PstI C_TGCA'G Cuts at: 830 2196 2211 3378 3554 3663 3723 4451 4793 Size: 1366 15 1167 176 109 60 728 342 Cuts at: 4793 830 Size: 3478 Fragments arranged by size: 3478 1366 1167 728 342 176 109 60 15 PvuI CG_AT'CG Cuts at: 2001 3433 2001 Size: 1432 6009 PvuII CAG'CTG Cuts at: 2464 2527 3664 4403 4654 2464 Size: 63 1137 739 251 5251 Fragments arranged by size: 5251 1137 739 251 63 RsrII CG'GwC_CG Cuts at: 4413 4413 Size: 7441 SacI G_AGCT'C Cuts at: 3405 3405 Size: 7441 SacII CC_GC'GG Cuts at: 3647 3647 Size: 7441 SalI G'TCGA_C Cuts at: 1936 4783 1936 Size: 2847 4594 SanDI GG'GwC_CC Cuts at: 4570 4570 Size: 7441 SapI GCTCTTCn'nnn_ Cuts at: 2118 3177 3738 2118 Size: 1059 561 5821 Fragments arranged by size: 5821 1059 561 SbfI CC_TGCA'GG Cuts at: 4793 4793 Size: 7441 ScaI AGT'ACT Cuts at: 5689 7401 5689 Size: 1712 5729 SexAI A'CCwGG_T Cuts at: 3016 3016 Size: 7441 SfcI C'TryA_G Cuts at: 826 1045 2192 2207 2898 3374 3550 3659 3719 Size: 219 1147 15 691 476 176 109 60 Cuts at: 3719 4447 4789 5924 6605 6796 826 Size: 728 342 1135 681 191 1471 Fragments arranged by size: 1471 1147 1135 728 691 681 476 342 219 191 176 109 60 15 SfiI GGCCn_nnn'nGGCC Cuts at: 2285 4464 2285 Size: 2179 5262 SfoI GGC'GCC Cuts at: 2168 3087 3866 2168 Size: 919 779 5743 Fragments arranged by size: 5743 919 779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7441 SmaI CCC'GGG Cuts at: 1872 2570 4768 1872 Size: 698 2198 4545 Fragments arranged by size: 4545 2198 698 SmlI C'TyrA_G Cuts at: 820 1017 1943 3848 4064 4554 5545 6446 6708 Size: 197 926 1905 216 490 991 901 262 Cuts at: 6708 820 Size: 1553 Fragments arranged by size: 1905 1553 991 926 901 490 262 216 197 SnaBI TAC'GTA Cuts at: 493 5232 493 Size: 4739 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7441 SphI G_CATG'C Cuts at: 1132 2246 3307 4799 1132 Size: 1114 1061 1492 3774 Fragments arranged by size: 3774 1492 1114 1061 SspI AAT'ATT Cuts at: 5 52 5365 5 Size: 47 5313 2081 Fragments arranged by size: 5313 2081 47 StuI AGG'CCT Cuts at: 1517 2288 1517 Size: 771 6670 StyI C'CwwG_G Cuts at: 513 1065 2004 2271 2689 3081 3339 3363 4880 Size: 552 939 267 418 392 258 24 1517 Cuts at: 4880 513 Size: 3074 Fragments arranged by size: 3074 1517 939 552 418 392 267 258 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2565 4430 5468 5485 5638 5823 6268 Size: 1255 985 1865 1038 17 153 185 445 Cuts at: 6268 325 Size: 1498 Fragments arranged by size: 1865 1498 1255 1038 985 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2676 5687 7399 Size: 274 80 33 51 1443 699 3011 1712 Cuts at: 7399 96 Size: 138 Fragments arranged by size: 3011 1712 1443 699 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2692 2724 2975 3007 7401 7433 2692 Size: 32 251 32 4394 32 2700 Fragments arranged by size: 4394 2700 251 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4237 1316 Size: 2921 4520 XbaI T'CTAG_A Cuts at: 4777 4777 Size: 7441 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2488 3943 4651 2488 Size: 1455 708 5278 Fragments arranged by size: 5278 1455 708 XhoI C'TCGA_G Cuts at: 1943 4554 1943 Size: 2611 4830 XmaI C'CCGG_G Cuts at: 1870 2568 4766 1870 Size: 698 2198 4545 Fragments arranged by size: 4545 2198 698 XmnI GAAnn'nnTTC Cuts at: 2288 2735 3383 5570 2288 Size: 447 648 2187 4159 Fragments arranged by size: 4159 2187 648 447 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4395 5249 276 Size: 53 83 186 936 2861 854 2468 Fragments arranged by size: 2861 2468 936 854 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI NruI NsiI PacI PspXI PsrI SrfI SwaI