(Linear) (Six-base) MAPSORT of: KIEE1537.seq Check: 3171 from: 1 to: 2736 KIEE1537.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 141 2736 Size: 141 2595 AatII G_ACGT'C Cuts at: 0 2392 2736 Size: 2392 344 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1681 2521 2736 Size: 1681 840 215 Fragments arranged by size: 1681 840 215 AhdI GACnn_n'nnGTC Cuts at: 0 714 2736 Size: 714 2022 AleI CACnn'nnGTG Cuts at: 0 1962 2736 Size: 1962 774 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1794 2122 2736 Size: 1794 328 614 Fragments arranged by size: 1794 614 328 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1588 1620 2736 Size: 1588 32 1116 Fragments arranged by size: 1588 1116 32 AlwNI CAG_nnn'CTG Cuts at: 0 144 513 1018 1692 2511 2736 Size: 144 369 505 674 819 225 Fragments arranged by size: 819 674 505 369 225 144 ApaI G_GGCC'C Cuts at: 0 265 1060 1957 2736 Size: 265 795 897 779 Fragments arranged by size: 897 795 779 265 ApoI r'AATT_y Cuts at: 0 1790 2668 2736 Size: 1790 878 68 Fragments arranged by size: 1790 878 68 AvaI C'yCGr_G Cuts at: 0 14 563 926 1123 2328 2339 2455 2549 Size: 14 549 363 197 1205 11 116 94 Cuts at: 2549 2736 Size: 187 Fragments arranged by size: 1205 549 363 197 187 116 94 14 11 BamHI G'GATC_C Cuts at: 0 2278 2736 Size: 2278 458 BanI G'GyrC_C Cuts at: 0 161 333 1080 1643 1859 2736 Size: 161 172 747 563 216 877 Fragments arranged by size: 877 747 563 216 172 161 BanII G_rGCy'C Cuts at: 0 256 265 836 948 1060 1123 1400 1516 Size: 256 9 571 112 112 63 277 116 Cuts at: 1516 1745 1957 2590 2736 Size: 229 212 633 146 Fragments arranged by size: 633 571 277 256 229 212 146 116 112 112 63 9 BbeI G_GCGC'C Cuts at: 0 165 1084 1863 2736 Size: 165 919 779 873 Fragments arranged by size: 919 873 779 165 BbsI GAAGACnn'nnnn_ Cuts at: 0 11 322 2534 2736 Size: 11 311 2212 202 Fragments arranged by size: 2212 311 202 11 BbvCI CC'TCA_GC Cuts at: 0 77 1508 1837 2353 2736 Size: 77 1431 329 516 383 Fragments arranged by size: 1431 516 383 329 77 BglI GCCn_nnn'nGGC Cuts at: 0 19 280 2459 2736 Size: 19 261 2179 277 Fragments arranged by size: 2179 277 261 19 BlpI GC'TnA_GC Cuts at: 0 793 1903 2623 2736 Size: 793 1110 720 113 Fragments arranged by size: 1110 793 720 113 Bme1580I G_kGCm'C Cuts at: 0 265 1060 1957 2736 Size: 265 795 897 779 Fragments arranged by size: 897 795 779 265 BmgBI CAC'GTC Cuts at: 0 1482 1803 1964 2736 Size: 1482 321 161 772 Fragments arranged by size: 1482 772 321 161 BmrI ACTGGGnnnn_n' Cuts at: 0 268 2736 Size: 268 2468 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 129 2365 2736 Size: 129 2236 371 Fragments arranged by size: 2236 371 129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 166 331 481 544 622 986 1051 Size: 124 42 165 150 63 78 364 65 Cuts at: 1051 1090 1151 1183 2017 2378 2671 2736 Size: 39 61 32 834 361 293 65 Fragments arranged by size: 834 364 361 293 165 150 124 78 65 65 63 61 42 39 32 Bpu10I CC'TnA_GC Cuts at: 0 77 1508 1837 2312 2353 2584 2736 Size: 77 1431 329 475 41 231 152 Fragments arranged by size: 1431 475 329 231 152 77 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1828 2044 2736 Size: 1828 216 692 Fragments arranged by size: 1828 692 216 BsaI GGTCTCn'nnnn_ Cuts at: 0 2629 2676 2736 Size: 2629 47 60 Fragments arranged by size: 2629 60 47 BsaAI yAC'GTr Cuts at: 0 195 576 1293 2736 Size: 195 381 717 1443 Fragments arranged by size: 1443 717 381 195 BsaHI Gr'CG_yC Cuts at: 0 162 496 1022 1081 1860 2083 2389 2736 Size: 162 334 526 59 779 223 306 347 Fragments arranged by size: 779 526 347 334 306 223 162 59 BsaWI w'CCGG_w Cuts at: 0 734 899 932 2736 Size: 734 165 33 1804 Fragments arranged by size: 1804 734 165 33 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 373 403 2736 Size: 373 30 2333 Fragments arranged by size: 2333 373 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 619 1378 1483 1524 2341 2537 2647 2668 Size: 619 759 105 41 817 196 110 21 Cuts at: 2668 2677 2736 Size: 9 59 Fragments arranged by size: 817 759 619 196 110 105 59 41 21 9 BseYI C'CCAG_C Cuts at: 0 165 249 518 1035 1384 2323 2589 2720 Size: 165 84 269 517 349 939 266 131 Cuts at: 2720 2736 Size: 16 Fragments arranged by size: 939 517 349 269 266 165 131 84 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 961 1204 1297 1639 2715 2736 Size: 436 525 243 93 342 1076 21 Fragments arranged by size: 1076 525 436 342 243 93 21 BsiEI CG_ry'CG Cuts at: 0 763 1156 1428 2736 Size: 763 393 272 1308 Fragments arranged by size: 1308 763 393 272 BsiHKAI G_wGCw'C Cuts at: 0 1400 2259 2736 Size: 1400 859 477 Fragments arranged by size: 1400 859 477 BsmI GAATG_Cn' Cuts at: 0 1348 1410 2736 Size: 1348 62 1326 Fragments arranged by size: 1348 1326 62 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1472 2380 2396 2736 Size: 1472 908 16 340 Fragments arranged by size: 1472 908 340 16 Bsp1286I G_dGCh'C Cuts at: 0 256 265 836 948 1060 1123 1400 1516 Size: 256 9 571 112 112 63 277 116 Cuts at: 1516 1745 1957 2259 2590 2736 Size: 229 212 302 331 146 Fragments arranged by size: 571 331 302 277 256 229 212 146 116 112 112 63 9 BspEI T'CCGG_A Cuts at: 0 734 2736 Size: 734 2002 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 141 429 1841 2027 2627 2736 Size: 141 288 1412 186 600 109 Fragments arranged by size: 1412 600 288 186 141 109 BsrBI CCG'CTC Cuts at: 0 570 585 2049 2241 2736 Size: 570 15 1464 192 495 Fragments arranged by size: 1464 570 495 192 15 BsrDI GCAATG_nn' Cuts at: 0 2379 2736 Size: 2379 357 BsrFI r'CCGG_y Cuts at: 0 336 452 755 2736 Size: 336 116 303 1981 Fragments arranged by size: 1981 336 303 116 BsrGI T'GTAC_A Cuts at: 0 671 2736 Size: 671 2065 BssSI C'ACGA_G Cuts at: 0 1948 2736 Size: 1948 788 BstAPI GCAn_nnn'nTGC Cuts at: 0 1227 2004 2736 Size: 1227 777 732 Fragments arranged by size: 1227 777 732 BstXI CCAn_nnnn'nTGG Cuts at: 0 1964 2736 Size: 1964 772 BstYI r'GATC_y Cuts at: 0 2278 2736 Size: 2278 458 BtgI C'CryG_G Cuts at: 0 446 650 1334 1639 1676 2474 2736 Size: 446 204 684 305 37 798 262 Fragments arranged by size: 798 684 446 305 262 204 37 BtsI GCAGTG_nn' Cuts at: 0 1994 2736 Size: 1994 742 EaeI y'GGCC_r Cuts at: 0 2153 2379 2736 Size: 2153 226 357 Fragments arranged by size: 2153 357 226 EarI CTCTTCn'nnn_ Cuts at: 0 113 1172 1327 1733 2736 Size: 113 1059 155 406 1003 Fragments arranged by size: 1059 1003 406 155 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 595 2124 2536 2736 Size: 595 1529 412 200 Fragments arranged by size: 1529 595 412 200 EcoICRI GAG'CTC Cuts at: 0 1398 2736 Size: 1398 1338 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 166 331 481 544 622 986 1051 Size: 124 42 165 150 63 78 364 65 Cuts at: 1051 1090 1151 1183 1681 2017 2378 2521 2671 Size: 39 61 32 498 336 361 143 150 Cuts at: 2671 2736 Size: 65 Fragments arranged by size: 498 364 361 336 165 150 150 143 124 78 65 65 63 61 42 39 32 EcoNI CCTnn'n_nnAGG Cuts at: 0 148 1465 2736 Size: 148 1317 1271 Fragments arranged by size: 1317 1271 148 EcoO109I rG'GnC_Cy Cuts at: 0 34 217 262 1056 1596 1830 1953 2452 Size: 34 183 45 794 540 234 123 499 Cuts at: 2452 2542 2565 2736 Size: 90 23 171 Fragments arranged by size: 794 540 499 234 183 171 123 90 45 34 23 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 300 2736 Size: 300 2436 FspI TGC'GCA Cuts at: 0 1297 2736 Size: 1297 1439 FspAI rTGC'GCAy Cuts at: 0 1297 2736 Size: 1297 1439 HaeII r_GCGC'y Cuts at: 0 165 1084 1863 2040 2736 Size: 165 919 779 177 696 Fragments arranged by size: 919 779 696 177 165 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 373 405 700 732 909 941 2736 Size: 373 32 295 32 177 32 1795 Fragments arranged by size: 1795 373 295 177 32 32 32 HincII GTy'rAC Cuts at: 0 657 1806 2736 Size: 657 1149 930 Fragments arranged by size: 1149 930 657 KasI G'GCGC_C Cuts at: 0 161 1080 1859 2736 Size: 161 919 779 877 Fragments arranged by size: 919 877 779 161 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 743 2216 2639 2736 Size: 743 1473 423 97 Fragments arranged by size: 1473 743 423 97 MscI TGG'CCA Cuts at: 0 2155 2736 Size: 2155 581 MslI CAynn'nnrTG Cuts at: 0 490 1405 1681 1962 2736 Size: 490 915 276 281 774 Fragments arranged by size: 915 774 490 281 276 MspA1I CmG'CkG Cuts at: 0 322 405 459 522 818 1641 1659 1689 Size: 322 83 54 63 296 823 18 30 Cuts at: 1689 1857 2398 2649 2736 Size: 168 541 251 87 Fragments arranged by size: 823 541 322 296 251 168 87 83 63 54 30 18 NaeI GCC'GGC Cuts at: 0 454 2736 Size: 454 2282 NarI GG'CG_CC Cuts at: 0 162 1081 1860 2736 Size: 162 919 779 876 Fragments arranged by size: 919 876 779 162 NcoI C'CATG_G Cuts at: 0 1334 2736 Size: 1334 1402 NgoMIV G'CCGG_C Cuts at: 0 452 2736 Size: 452 2284 NspI r_CATG'y Cuts at: 0 241 249 678 1302 2002 2736 Size: 241 8 429 624 700 734 Fragments arranged by size: 734 700 624 429 241 8 PasI CC'CwG_GG Cuts at: 0 500 2569 2736 Size: 500 2069 167 Fragments arranged by size: 2069 500 167 PflMI CCAn_nnn'nTGG Cuts at: 0 144 177 1335 2736 Size: 144 33 1158 1401 Fragments arranged by size: 1401 1158 144 33 PfoI T'CCnGG_A Cuts at: 0 287 308 803 2609 2736 Size: 287 21 495 1806 127 Fragments arranged by size: 1806 495 287 127 21 PmlI CAC'GTG Cuts at: 0 195 576 1293 2736 Size: 195 381 717 1443 Fragments arranged by size: 1443 717 381 195 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2336 2368 2736 Size: 2336 32 368 Fragments arranged by size: 2336 368 32 PpuMI rG'GwC_Cy Cuts at: 0 217 1596 1830 2565 2736 Size: 217 1379 234 735 171 Fragments arranged by size: 1379 735 234 217 171 PspOMI G'GGCC_C Cuts at: 0 261 1056 1953 2736 Size: 261 795 897 783 Fragments arranged by size: 897 795 783 261 PstI C_TGCA'G Cuts at: 0 191 206 1373 1549 1658 1718 2446 2736 Size: 191 15 1167 176 109 60 728 290 Fragments arranged by size: 1167 728 290 191 176 109 60 15 PvuI CG_AT'CG Cuts at: 0 1428 2736 Size: 1428 1308 PvuII CAG'CTG Cuts at: 0 459 522 1659 2398 2649 2736 Size: 459 63 1137 739 251 87 Fragments arranged by size: 1137 739 459 251 87 63 RsrII CG'GwC_CG Cuts at: 0 2408 2736 Size: 2408 328 SacI G_AGCT'C Cuts at: 0 1400 2736 Size: 1400 1336 SacII CC_GC'GG Cuts at: 0 1642 2736 Size: 1642 1094 SanDI GG'GwC_CC Cuts at: 0 2565 2736 Size: 2565 171 SapI GCTCTTCn'nnn_ Cuts at: 0 113 1172 1733 2736 Size: 113 1059 561 1003 Fragments arranged by size: 1059 1003 561 113 SexAI A'CCwGG_T Cuts at: 0 1011 2736 Size: 1011 1725 SfcI C'TryA_G Cuts at: 0 187 202 893 1369 1545 1654 1714 2442 Size: 187 15 691 476 176 109 60 728 Cuts at: 2442 2736 Size: 294 Fragments arranged by size: 728 691 476 294 187 176 109 60 15 SfiI GGCCn_nnn'nGGCC Cuts at: 0 280 2459 2736 Size: 280 2179 277 Fragments arranged by size: 2179 280 277 SfoI GGC'GCC Cuts at: 0 163 1082 1861 2736 Size: 163 919 779 875 Fragments arranged by size: 919 875 779 163 SmaI CCC'GGG Cuts at: 0 565 2736 Size: 565 2171 SmlI C'TyrA_G Cuts at: 0 1843 2059 2549 2736 Size: 1843 216 490 187 Fragments arranged by size: 1843 490 216 187 SphI G_CATG'C Cuts at: 0 241 1302 2736 Size: 241 1061 1434 Fragments arranged by size: 1434 1061 241 StuI AGG'CCT Cuts at: 0 283 2736 Size: 283 2453 StyI C'CwwG_G Cuts at: 0 266 684 1076 1334 1358 2736 Size: 266 418 392 258 24 1378 Fragments arranged by size: 1378 418 392 266 258 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 560 2425 2736 Size: 560 1865 311 Fragments arranged by size: 1865 560 311 TatI w'GTAC_w Cuts at: 0 671 2736 Size: 671 2065 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 687 719 970 1002 2736 Size: 687 32 251 32 1734 Fragments arranged by size: 1734 687 251 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2232 2736 Size: 2232 504 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 483 1938 2646 2736 Size: 483 1455 708 90 Fragments arranged by size: 1455 708 483 90 XhoI C'TCGA_G Cuts at: 0 2549 2736 Size: 2549 187 XmaI C'CCGG_G Cuts at: 0 563 2736 Size: 563 2173 XmnI GAAnn'nnTTC Cuts at: 0 283 730 1378 2736 Size: 283 447 648 1358 Fragments arranged by size: 1358 648 447 283 ZraI GAC'GTC Cuts at: 0 2390 2736 Size: 2390 346 Enzymes that do cut: AarI AatII AcuI AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SanDI SapI SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BspHI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI SalI SbfI ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI