(Circular) (Six-base) MAPSORT of: pFN21AE1434.seq Check: 9043 from: 1 to: 7057 pFN21AE1434 7057 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4867 278 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4400 1937 Size: 2463 4594 Acc65I G'GTAC_C Cuts at: 4378 4378 Size: 7057 AclI AA'CG_TT Cuts at: 1563 2936 5184 5557 1563 Size: 1373 2248 373 3063 Fragments arranged by size: 3063 2248 1373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2538 3599 5120 6504 6772 1632 Size: 906 1061 1521 1384 268 1917 Fragments arranged by size: 1917 1521 1384 1061 906 268 AfeI AGC'GCT Cuts at: 4247 4247 Size: 7057 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6860 AflIII A'CryG_T Cuts at: 1203 5956 6933 1203 Size: 4753 977 1327 Fragments arranged by size: 4753 1327 977 AgeI A'CCGG_T Cuts at: 5888 5888 Size: 7057 AhdI GACnn_n'nnGTC Cuts at: 5786 5786 Size: 7057 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7057 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7057 AlwNI CAG_nnn'CTG Cuts at: 2162 2245 3991 4800 6372 2162 Size: 83 1746 809 1572 2847 Fragments arranged by size: 2847 1746 1572 809 83 ApaLI G'TGCA_C Cuts at: 5114 6270 5114 Size: 1156 5901 ApoI r'AATT_y Cuts at: 1493 2746 3112 3139 3384 3427 3623 4366 4592 Size: 1253 366 27 245 43 196 743 226 Cuts at: 4592 4744 6853 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1253 743 366 245 226 196 152 43 27 AseI AT'TA_AT Cuts at: 160 1032 5611 160 Size: 872 4579 1606 Fragments arranged by size: 4579 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 AvaI C'yCGr_G Cuts at: 1870 1943 4382 1870 Size: 73 2439 4545 Fragments arranged by size: 4545 2439 73 AvrII C'CTAG_G Cuts at: 2419 2419 Size: 7057 BamHI G'GATC_C Cuts at: 4387 4754 4387 Size: 367 6690 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4378 5833 618 Size: 325 217 627 2591 1455 1842 Fragments arranged by size: 2591 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2154 4221 4376 1391 Size: 763 2067 155 4072 Fragments arranged by size: 4072 2067 763 155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2346 3587 4016 928 Size: 971 447 1241 429 3969 Fragments arranged by size: 3969 1241 971 447 429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5248 5282 1903 Size: 34 50 34 3227 34 3678 Fragments arranged by size: 3678 3227 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4949 6159 4949 Size: 1210 5847 BclI T'GATC_A Cuts at: 1548 1548 Size: 7057 BfrBI ATG'CAT Cuts at: 3932 3932 Size: 7057 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5668 136 Size: 107 122 71 631 605 3996 1525 Fragments arranged by size: 3996 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2557 7052 2557 Size: 4495 2562 BlpI GC'TnA_GC Cuts at: 4311 4474 4311 Size: 163 6894 Bme1580I G_kGCm'C Cuts at: 1790 5118 6274 1790 Size: 3328 1156 2573 Fragments arranged by size: 3328 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7057 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2408 3973 4788 Size: 416 53 83 399 24 991 1565 815 Cuts at: 4788 5746 442 Size: 958 1753 Fragments arranged by size: 1753 1565 991 958 815 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7057 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2666 3863 4013 4235 4310 5717 1239 Size: 983 444 1197 150 222 75 1407 2579 Fragments arranged by size: 2579 1407 1197 983 444 222 150 75 Bpu10I CC'TnA_GC Cuts at: 2122 2122 Size: 7057 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2552 2561 5182 6047 6345 2552 Size: 9 2621 865 298 3264 Fragments arranged by size: 3264 2621 865 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5720 882 Size: 617 4221 2219 Fragments arranged by size: 4221 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4848 493 Size: 711 3644 2702 Fragments arranged by size: 3644 2702 711 BsaBI GATnn'nnATC Cuts at: 7051 7051 Size: 7057 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4864 5246 5881 275 Size: 53 83 186 936 3331 382 635 1451 Fragments arranged by size: 3331 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2946 3218 5490 5888 6162 6309 6720 2946 Size: 272 2272 398 274 147 411 3283 Fragments arranged by size: 3283 2272 411 398 274 272 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6832 Fragments arranged by size: 6832 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7057 BseYI C'CCAG_C Cuts at: 3908 4242 6260 6756 3908 Size: 334 2018 496 4209 Fragments arranged by size: 4209 2018 496 334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6484 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4507 5268 6296 1540 Size: 275 186 2506 761 1028 2301 Fragments arranged by size: 2506 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2993 3796 5118 5203 6274 2993 Size: 803 1322 85 1071 3776 Fragments arranged by size: 3776 1322 1071 803 85 BsmI GAATG_Cn' Cuts at: 3366 3535 4568 4661 3366 Size: 169 1033 93 5762 Fragments arranged by size: 5762 1033 169 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2993 3796 4221 4376 5118 5203 Size: 399 364 839 803 425 155 742 85 Cuts at: 5203 6274 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 839 803 742 425 399 364 155 85 BspEI T'CCGG_A Cuts at: 2946 6720 2946 Size: 3774 3283 BspHI T'CATG_A Cuts at: 4946 6676 4946 Size: 1730 5327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3715 4412 844 Size: 2871 697 3489 Fragments arranged by size: 3489 2871 697 BsrBI CCG'CTC Cuts at: 1394 1823 3952 4944 1394 Size: 429 2129 992 3507 Fragments arranged by size: 3507 2129 992 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2107 5552 5726 65 Size: 1183 859 3445 174 1396 Fragments arranged by size: 3445 1396 1183 859 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5701 5888 1700 Size: 110 3891 187 2869 Fragments arranged by size: 3891 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7057 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7057 BssSI C'ACGA_G Cuts at: 5117 6129 5117 Size: 1012 6045 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7057 BstBI TT'CG_AA Cuts at: 5861 5861 Size: 7057 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7057 BstXI CCAn_nnnn'nTGG Cuts at: 3379 3716 3379 Size: 337 6720 BstYI r'GATC_y Cuts at: 1972 2126 2557 2917 3100 4387 4754 5139 5156 Size: 154 431 360 183 1287 367 385 17 Cuts at: 5156 6608 6694 6706 7052 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1287 431 385 367 360 346 183 154 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2146 2146 Size: 7057 BtgI C'CryG_G Cuts at: 513 1065 2673 3841 4299 513 Size: 552 1608 1168 458 3271 Fragments arranged by size: 3271 1608 1168 552 458 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6318 Fragments arranged by size: 6318 683 56 BtsI GCAGTG_nn' Cuts at: 4573 5367 5387 4573 Size: 794 20 6243 Fragments arranged by size: 6243 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7057 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 1998 2033 2655 2690 529 Size: 35 1434 35 622 35 4896 Fragments arranged by size: 4896 1434 622 35 35 35 DraI TTT'AAA Cuts at: 2133 3081 4361 2133 Size: 948 1280 4829 Fragments arranged by size: 4829 1280 948 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6064 809 Size: 507 4748 1802 Fragments arranged by size: 4748 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3305 3657 3677 Size: 54 1425 50 275 18 1475 352 20 Cuts at: 3677 3718 4504 5393 7022 8 Size: 41 786 889 1629 43 Fragments arranged by size: 1629 1475 1425 889 786 352 275 54 50 43 41 20 18 EagI C'GGCC_G Cuts at: 1537 1812 4504 1537 Size: 275 2692 4090 Fragments arranged by size: 4090 2692 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4124 4987 1353 Size: 2771 863 3423 Fragments arranged by size: 3423 2771 863 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3728 5644 6018 6164 387 Size: 1064 138 2139 1916 374 146 1280 Fragments arranged by size: 2139 1916 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2538 2666 3599 3863 4013 4235 Size: 393 590 316 128 933 264 150 222 Cuts at: 4235 4310 5120 5717 6504 6772 1239 Size: 75 810 597 787 268 1524 Fragments arranged by size: 1524 933 810 787 597 590 393 316 268 264 222 150 128 75 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2057 Size: 7057 EcoO109I rG'GnC_Cy Cuts at: 3823 3823 Size: 7057 EcoRI G'AATT_C Cuts at: 3623 4366 4744 3623 Size: 743 378 5936 Fragments arranged by size: 5936 743 378 EcoRV GAT'ATC Cuts at: 3262 3262 Size: 7057 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4054 4054 Size: 7057 FspI TGC'GCA Cuts at: 5563 5563 Size: 7057 HaeII r_GCGC'y Cuts at: 1678 4249 6204 1678 Size: 2571 1955 2531 Fragments arranged by size: 2571 2531 1955 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3689 3721 5698 5730 5772 Size: 32 135 32 2117 32 1977 32 42 Cuts at: 5772 5804 1373 Size: 32 2626 Fragments arranged by size: 2626 2117 1977 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 4401 4647 669 Size: 988 281 1002 1461 246 3079 Fragments arranged by size: 3079 1461 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7057 HpaI GTT'AAC Cuts at: 2940 4647 2940 Size: 1707 5350 KpnI G_GTAC'C Cuts at: 4382 4382 Size: 7057 MfeI C'AATT_G Cuts at: 4656 4656 Size: 7057 MluI A'CGCG_T Cuts at: 6933 6933 Size: 7057 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 3732 6171 6355 1305 Size: 903 1524 2439 184 2007 Fragments arranged by size: 2439 2007 1524 903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3307 3659 3679 3720 10 Size: 54 1425 343 1475 352 20 41 3347 Fragments arranged by size: 3347 1475 1425 352 343 54 41 20 MslI CAynn'nnrTG Cuts at: 518 1166 3714 5015 5374 5533 518 Size: 648 2548 1301 359 159 2042 Fragments arranged by size: 2548 2042 1301 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4315 4473 5150 6298 6543 1606 Size: 2709 158 677 1148 245 2120 Fragments arranged by size: 2709 2120 1148 677 245 158 NcoI C'CATG_G Cuts at: 513 1065 3841 4299 513 Size: 552 2776 458 3271 Fragments arranged by size: 3271 2776 552 458 NdeI CA'TA_TG Cuts at: 387 2622 387 Size: 2235 4822 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7057 NotI GC'GGCC_GC Cuts at: 4504 4504 Size: 7057 NsiI A_TGCA'T Cuts at: 3934 3934 Size: 7057 NspI r_CATG'y Cuts at: 1132 1229 3787 4415 5960 1132 Size: 97 2558 628 1545 2229 Fragments arranged by size: 2558 2229 1545 628 97 PasI CC'CwG_GG Cuts at: 1348 2299 1348 Size: 951 6106 PciI A'CATG_T Cuts at: 5956 5956 Size: 7057 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 3991 1108 Size: 1137 1746 4174 Fragments arranged by size: 4174 1746 1137 PfoI T'CCnGG_A Cuts at: 3984 3984 Size: 7057 PmeI GTTT'AAAC Cuts at: 4361 4361 Size: 7057 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5126 5158 6639 6671 5126 Size: 32 1481 32 5512 Fragments arranged by size: 5512 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3823 3823 Size: 7057 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7057 PsiI TTA'TAA Cuts at: 4627 4627 Size: 7057 PstI C_TGCA'G Cuts at: 830 4409 830 Size: 3579 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7057 SalI G'TCGA_C Cuts at: 1936 4399 1936 Size: 2463 4594 SbfI CC_TGCA'GG Cuts at: 4409 4409 Size: 7057 ScaI AGT'ACT Cuts at: 5305 7017 5305 Size: 1712 5345 SfcI C'TryA_G Cuts at: 826 1045 2219 2561 4405 5540 6221 6412 826 Size: 219 1174 342 1844 1135 681 191 1471 Fragments arranged by size: 1844 1471 1174 1135 681 342 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7057 SmaI CCC'GGG Cuts at: 1872 4384 1872 Size: 2512 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 2540 5161 6062 6324 820 Size: 197 926 588 9 2621 901 262 1553 Fragments arranged by size: 2621 1553 926 901 588 262 197 9 SnaBI TAC'GTA Cuts at: 493 4848 493 Size: 4355 2702 SpeI A'CTAG_T Cuts at: 152 3508 152 Size: 3356 3701 SphI G_CATG'C Cuts at: 1132 4415 1132 Size: 3283 3774 SspI AAT'ATT Cuts at: 5 52 3323 4981 5 Size: 47 3271 1658 2081 Fragments arranged by size: 3271 2081 1658 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7057 StyI C'CwwG_G Cuts at: 513 1065 2058 2419 2963 3841 4299 4496 513 Size: 552 993 361 544 878 458 197 3074 Fragments arranged by size: 3074 993 878 552 544 458 361 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3955 5084 5101 5254 5439 5884 325 Size: 1255 2375 1129 17 153 185 445 1498 Fragments arranged by size: 2375 1498 1255 1129 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5303 7015 96 Size: 274 80 33 51 1443 3326 1712 138 Fragments arranged by size: 3326 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7017 7049 7017 Size: 32 7025 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7057 XbaI T'CTAG_A Cuts at: 4393 4393 Size: 7057 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2016 3839 4285 2016 Size: 1823 446 4788 Fragments arranged by size: 4788 1823 446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7057 XmaI C'CCGG_G Cuts at: 1870 4382 1870 Size: 2512 4545 XmnI GAAnn'nnTTC Cuts at: 5186 5186 Size: 7057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4865 276 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI