Restriction map for
pFN21AE1434
(Linear) (Six-base) MAPSORT of: KIEE1434.seq Check: 3805 from: 1 to: 2352
KIEE1434.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AclI AA'CG_TT
Cuts at: 0 931 2352
Size: 931 1421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1594 2352
Size: 533 1061 758
Fragments arranged by size:
1061 758 533
AfeI AGC'GCT
Cuts at: 0 2242 2352
Size: 2242 110
AlwNI CAG_nnn'CTG
Cuts at: 0 157 240 1986 2352
Size: 157 83 1746 366
Fragments arranged by size:
1746 366 157 83
ApoI r'AATT_y
Cuts at: 0 741 1107 1134 1379 1422 1618 2352
Size: 741 366 27 245 43 196 734
Fragments arranged by size:
741 734 366 245 196 43 27
AvrII C'CTAG_G
Cuts at: 0 414 2352
Size: 414 1938
BanII G_rGCy'C
Cuts at: 0 149 2216 2352
Size: 149 2067 136
Fragments arranged by size:
2067 149 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 341 1582 2011 2352
Size: 341 1241 429 341
Fragments arranged by size:
1241 429 341 341
BfrBI ATG'CAT
Cuts at: 0 1927 2352
Size: 1927 425
BglII A'GATC_T
Cuts at: 0 552 2352
Size: 552 1800
BlpI GC'TnA_GC
Cuts at: 0 2306 2352
Size: 2306 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 403 1968 2352
Size: 403 1565 384
Fragments arranged by size:
1565 403 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 661 1858 2008 2230 2305 2352
Size: 217 444 1197 150 222 75 47
Fragments arranged by size:
1197 444 222 217 150 75 47
Bpu10I CC'TnA_GC
Cuts at: 0 117 2352
Size: 117 2235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 556 2352
Size: 547 9 1796
Fragments arranged by size:
1796 547 9
BsaWI w'CCGG_w
Cuts at: 0 941 1213 2352
Size: 941 272 1139
Fragments arranged by size:
1139 941 272
BseYI C'CCAG_C
Cuts at: 0 1903 2237 2352
Size: 1903 334 115
Fragments arranged by size:
1903 334 115
BsiHKAI G_wGCw'C
Cuts at: 0 988 1791 2352
Size: 988 803 561
Fragments arranged by size:
988 803 561
BsmI GAATG_Cn'
Cuts at: 0 1361 1530 2352
Size: 1361 169 822
Fragments arranged by size:
1361 822 169
Bsp1286I G_dGCh'C
Cuts at: 0 149 988 1791 2216 2352
Size: 149 839 803 425 136
Fragments arranged by size:
839 803 425 149 136
BspEI T'CCGG_A
Cuts at: 0 941 2352
Size: 941 1411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1710 2352
Size: 1710 642
BsrBI CCG'CTC
Cuts at: 0 1947 2352
Size: 1947 405
BsrDI GCAATG_nn'
Cuts at: 0 102 2352
Size: 102 2250
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1374 1711 2352
Size: 1374 337 641
Fragments arranged by size:
1374 641 337
BstYI r'GATC_y
Cuts at: 0 121 552 912 1095 2352
Size: 121 431 360 183 1257
Fragments arranged by size:
1257 431 360 183 121
Bsu36I CC'TnA_GG
Cuts at: 0 141 2352
Size: 141 2211
BtgI C'CryG_G
Cuts at: 0 668 1836 2294 2352
Size: 668 1168 458 58
Fragments arranged by size:
1168 668 458 58
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 28 650 685 2352
Size: 28 622 35 1667
Fragments arranged by size:
1667 622 35 28
DraI TTT'AAA
Cuts at: 0 128 1076 2352
Size: 128 948 1276
Fragments arranged by size:
1276 948 128
EaeI y'GGCC_r
Cuts at: 0 1300 1652 1672 1713 2352
Size: 1300 352 20 41 639
Fragments arranged by size:
1300 639 352 41 20
EarI CTCTTCn'nnn_
Cuts at: 0 2119 2352
Size: 2119 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1723 2352
Size: 1723 629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 533 661 1594 1858 2008 2230 2305
Size: 217 316 128 933 264 150 222 75
Cuts at: 2305 2352
Size: 47
Fragments arranged by size:
933 316 264 222 217 150 128 75
47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 2352
Size: 52 2300
EcoO109I rG'GnC_Cy
Cuts at: 0 1818 2352
Size: 1818 534
EcoRI G'AATT_C
Cuts at: 0 1618 2352
Size: 1618 734
EcoRV GAT'ATC
Cuts at: 0 1257 2352
Size: 1257 1095
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2049 2352
Size: 2049 303
HaeII r_GCGC'y
Cuts at: 0 2244 2352
Size: 2244 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1684 1716 2352
Size: 1684 32 636
Fragments arranged by size:
1684 636 32
HincII GTy'rAC
Cuts at: 0 935 2352
Size: 935 1417
HpaI GTT'AAC
Cuts at: 0 935 2352
Size: 935 1417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1727 2352
Size: 203 1524 625
Fragments arranged by size:
1524 625 203
MscI TGG'CCA
Cuts at: 0 1302 1654 1674 1715 2352
Size: 1302 352 20 41 637
Fragments arranged by size:
1302 637 352 41 20
MslI CAynn'nnrTG
Cuts at: 0 1709 2352
Size: 1709 643
MspA1I CmG'CkG
Cuts at: 0 2310 2352
Size: 2310 42
NcoI C'CATG_G
Cuts at: 0 1836 2294 2352
Size: 1836 458 58
Fragments arranged by size:
1836 458 58
NdeI CA'TA_TG
Cuts at: 0 617 2352
Size: 617 1735
NsiI A_TGCA'T
Cuts at: 0 1929 2352
Size: 1929 423
NspI r_CATG'y
Cuts at: 0 1782 2352
Size: 1782 570
PasI CC'CwG_GG
Cuts at: 0 294 2352
Size: 294 2058
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 1986 2352
Size: 240 1746 366
Fragments arranged by size:
1746 366 240
PfoI T'CCnGG_A
Cuts at: 0 1979 2352
Size: 1979 373
PpuMI rG'GwC_Cy
Cuts at: 0 1818 2352
Size: 1818 534
SfcI C'TryA_G
Cuts at: 0 214 556 2352
Size: 214 342 1796
Fragments arranged by size:
1796 342 214
SmlI C'TyrA_G
Cuts at: 0 526 535 2352
Size: 526 9 1817
Fragments arranged by size:
1817 526 9
SpeI A'CTAG_T
Cuts at: 0 1503 2352
Size: 1503 849
SspI AAT'ATT
Cuts at: 0 1318 2352
Size: 1318 1034
StyI C'CwwG_G
Cuts at: 0 53 414 958 1836 2294 2352
Size: 53 361 544 878 458 58
Fragments arranged by size:
878 544 458 361 58 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1950 2352
Size: 1950 402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 11 1834 2280 2352
Size: 11 1823 446 72
Fragments arranged by size:
1823 446 72 11
Enzymes that do cut:
AclI AcuI AfeI AlwNI ApoI AvrII BanII BbsI
BfrBI BglII BlpI BmrI BpmI Bpu10I BpuEI BsaWI
BseYI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BstXI BstYI Bsu36I BtgI CspCI DraI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PflMI PfoI PpuMI SfcI
SmlI SpeI SspI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BglI Bme1580I BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmBI
BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI DraIII DrdI EagI EcoICRI
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI