(Linear) (Six-base) MAPSORT of: KIEE3633.seq Check: 8751 from: 1 to: 2466 KIEE3633.orf With 182 enzymes: SgfI * March 22, 2012 16:38 .. AccI GT'mk_AC Cuts at: 0 1772 2426 2441 2466 Size: 1772 654 15 25 Fragments arranged by size: 1772 654 25 15 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1017 1581 1654 1702 2466 Size: 1017 564 73 48 764 Fragments arranged by size: 1017 764 564 73 48 AfeI AGC'GCT Cuts at: 0 847 2466 Size: 847 1619 AflIII A'CryG_T Cuts at: 0 1175 1177 2466 Size: 1175 2 1289 Fragments arranged by size: 1289 1175 2 AleI CACnn'nnGTG Cuts at: 0 1667 2466 Size: 1667 799 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 976 2466 Size: 976 1490 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 205 237 2466 Size: 205 32 2229 Fragments arranged by size: 2229 205 32 AlwNI CAG_nnn'CTG Cuts at: 0 444 525 708 1119 2382 2466 Size: 444 81 183 411 1263 84 Fragments arranged by size: 1263 444 411 183 84 81 AvaI C'yCGr_G Cuts at: 0 806 943 1603 1834 2466 Size: 806 137 660 231 632 Fragments arranged by size: 806 660 632 231 137 BamHI G'GATC_C Cuts at: 0 366 2466 Size: 366 2100 BanI G'GyrC_C Cuts at: 0 93 2131 2466 Size: 93 2038 335 Fragments arranged by size: 2038 335 93 BanII G_rGCy'C Cuts at: 0 164 314 655 1031 1480 2126 2466 Size: 164 150 341 376 449 646 340 Fragments arranged by size: 646 449 376 341 340 164 150 BbsI GAAGACnn'nnnn_ Cuts at: 0 113 2466 Size: 113 2353 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 283 317 2466 Size: 283 34 2149 Fragments arranged by size: 2149 283 34 BglI GCCn_nnn'nGGC Cuts at: 0 1213 1858 2227 2466 Size: 1213 645 369 239 Fragments arranged by size: 1213 645 369 239 BlpI GC'TnA_GC Cuts at: 0 727 2466 Size: 727 1739 Bme1580I G_kGCm'C Cuts at: 0 430 583 2466 Size: 430 153 1883 Fragments arranged by size: 1883 430 153 BmgBI CAC'GTC Cuts at: 0 1941 2466 Size: 1941 525 BmrI ACTGGGnnnn_n' Cuts at: 0 309 1446 1975 2357 2466 Size: 309 1137 529 382 109 Fragments arranged by size: 1137 529 382 309 109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1434 1575 2226 2319 2466 Size: 1434 141 651 93 147 Fragments arranged by size: 1434 651 147 141 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 373 415 730 778 1114 1165 1270 Size: 232 141 42 315 48 336 51 105 Cuts at: 1270 1411 1846 1876 2053 2314 2466 Size: 141 435 30 177 261 152 Fragments arranged by size: 435 336 315 261 232 177 152 141 141 105 51 48 42 30 Bpu10I CC'TnA_GC Cuts at: 0 437 2466 Size: 437 2029 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 1066 1117 1870 2466 Size: 561 505 51 753 596 Fragments arranged by size: 753 596 561 505 51 BsaAI yAC'GTr Cuts at: 0 981 1178 2466 Size: 981 197 1288 Fragments arranged by size: 1288 981 197 BsaBI GATnn'nnATC Cuts at: 0 1460 2466 Size: 1460 1006 BsaHI Gr'CG_yC Cuts at: 0 1169 2438 2466 Size: 1169 1269 28 Fragments arranged by size: 1269 1169 28 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 22 52 205 235 832 862 2466 Size: 22 30 153 30 597 30 1604 Fragments arranged by size: 1604 597 153 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 172 229 1039 1741 1765 2134 2164 2167 Size: 172 57 810 702 24 369 30 3 Cuts at: 2167 2257 2260 2466 Size: 90 3 206 Fragments arranged by size: 810 702 369 206 172 90 57 30 24 3 3 BseYI C'CCAG_C Cuts at: 0 108 1318 2466 Size: 108 1210 1148 Fragments arranged by size: 1210 1148 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 158 683 690 778 1567 2339 2466 Size: 158 525 7 88 789 772 127 Fragments arranged by size: 789 772 525 158 127 88 7 BsiEI CG_ry'CG Cuts at: 0 1575 1956 2435 2466 Size: 1575 381 479 31 Fragments arranged by size: 1575 479 381 31 BsiHKAI G_wGCw'C Cuts at: 0 85 164 438 1031 1480 2186 2466 Size: 85 79 274 593 449 706 280 Fragments arranged by size: 706 593 449 280 274 85 79 BsmI GAATG_Cn' Cuts at: 0 282 2466 Size: 282 2184 Bsp1286I G_dGCh'C Cuts at: 0 85 164 314 430 438 583 655 1031 Size: 85 79 150 116 8 145 72 376 Cuts at: 1031 1480 2126 2186 2466 Size: 449 646 60 280 Fragments arranged by size: 646 449 376 280 150 145 116 85 79 72 60 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 383 2381 2402 2466 Size: 383 1998 21 64 Fragments arranged by size: 1998 383 64 21 BsrBI CCG'CTC Cuts at: 0 762 882 1559 1608 2157 2466 Size: 762 120 677 49 549 309 Fragments arranged by size: 762 677 549 309 120 49 BsrFI r'CCGG_y Cuts at: 0 85 99 614 629 638 949 2434 2466 Size: 85 14 515 15 9 311 1485 32 Fragments arranged by size: 1485 515 311 85 32 15 14 9 BssSI C'ACGA_G Cuts at: 0 1123 2466 Size: 1123 1343 BstAPI GCAn_nnn'nTGC Cuts at: 0 1119 2109 2382 2466 Size: 1119 990 273 84 Fragments arranged by size: 1119 990 273 84 BstEII G'GTnAC_C Cuts at: 0 801 1929 1974 2466 Size: 801 1128 45 492 Fragments arranged by size: 1128 801 492 45 BstXI CCAn_nnnn'nTGG Cuts at: 0 1436 1941 2466 Size: 1436 505 525 Fragments arranged by size: 1436 525 505 BstYI r'GATC_y Cuts at: 0 366 1613 2466 Size: 366 1247 853 Fragments arranged by size: 1247 853 366 Bsu36I CC'TnA_GG Cuts at: 0 1282 2162 2466 Size: 1282 880 304 Fragments arranged by size: 1282 880 304 BtgI C'CryG_G Cuts at: 0 192 1238 1934 2063 2466 Size: 192 1046 696 129 403 Fragments arranged by size: 1046 696 403 192 129 BtsI GCAGTG_nn' Cuts at: 0 681 2466 Size: 681 1785 DraIII CAC_nnn'GTG Cuts at: 0 801 2466 Size: 801 1665 EaeI y'GGCC_r Cuts at: 0 1063 1568 1741 1801 1949 2360 2432 2466 Size: 1063 505 173 60 148 411 72 34 Fragments arranged by size: 1063 505 411 173 148 72 60 34 EagI C'GGCC_G Cuts at: 0 2432 2466 Size: 2432 34 EarI CTCTTCn'nnn_ Cuts at: 0 149 1036 1546 2263 2466 Size: 149 887 510 717 203 Fragments arranged by size: 887 717 510 203 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 37 1261 1708 2097 2466 Size: 37 1224 447 389 369 Fragments arranged by size: 1224 447 389 369 37 EcoICRI GAG'CTC Cuts at: 0 162 1029 1478 2466 Size: 162 867 449 988 Fragments arranged by size: 988 867 449 162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 373 415 730 778 1017 1114 1165 Size: 232 141 42 315 48 239 97 51 Cuts at: 1165 1270 1411 1581 1654 1702 1846 1876 2053 Size: 105 141 170 73 48 144 30 177 Cuts at: 2053 2314 2466 Size: 261 152 Fragments arranged by size: 315 261 239 232 177 170 152 144 141 141 105 97 73 51 48 48 42 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 796 2466 Size: 796 1670 EcoO109I rG'GnC_Cy Cuts at: 0 1077 1350 1440 1536 1653 1830 2466 Size: 1077 273 90 96 117 177 636 Fragments arranged by size: 1077 636 273 177 117 96 90 EcoRV GAT'ATC Cuts at: 0 1842 2466 Size: 1842 624 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1557 1659 2466 Size: 1557 102 807 Fragments arranged by size: 1557 807 102 FspI TGC'GCA Cuts at: 0 1018 2466 Size: 1018 1448 HaeII r_GCGC'y Cuts at: 0 849 975 1219 2071 2466 Size: 849 126 244 852 395 Fragments arranged by size: 852 849 395 244 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 22 54 440 472 507 539 2466 Size: 22 32 386 32 35 32 1927 Fragments arranged by size: 1927 386 35 32 32 32 22 HincII GTy'rAC Cuts at: 0 1173 2466 Size: 1173 1293 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1888 2466 Size: 1888 578 MslI CAynn'nnrTG Cuts at: 0 1667 1939 2338 2466 Size: 1667 272 399 128 Fragments arranged by size: 1667 399 272 128 MspA1I CmG'CkG Cuts at: 0 134 657 968 1119 2024 2466 Size: 134 523 311 151 905 442 Fragments arranged by size: 905 523 442 311 151 134 NaeI GCC'GGC Cuts at: 0 101 951 2436 2466 Size: 101 850 1485 30 Fragments arranged by size: 1485 850 101 30 NcoI C'CATG_G Cuts at: 0 1238 1934 2466 Size: 1238 696 532 Fragments arranged by size: 1238 696 532 NgoMIV G'CCGG_C Cuts at: 0 99 949 2434 2466 Size: 99 850 1485 32 Fragments arranged by size: 1485 850 99 32 NspI r_CATG'y Cuts at: 0 2328 2337 2466 Size: 2328 9 129 Fragments arranged by size: 2328 129 9 PasI CC'CwG_GG Cuts at: 0 1920 2466 Size: 1920 546 PflMI CCAn_nnn'nTGG Cuts at: 0 708 2466 Size: 708 1758 PfoI T'CCnGG_A Cuts at: 0 124 209 1247 1442 1721 2466 Size: 124 85 1038 195 279 745 Fragments arranged by size: 1038 745 279 195 124 85 PmlI CAC'GTG Cuts at: 0 981 1178 2466 Size: 981 197 1288 Fragments arranged by size: 1288 981 197 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 205 237 2466 Size: 205 32 2229 Fragments arranged by size: 2229 205 32 PpuMI rG'GwC_Cy Cuts at: 0 1077 1350 1440 1536 1830 2466 Size: 1077 273 90 96 294 636 Fragments arranged by size: 1077 636 294 273 96 90 PstI C_TGCA'G Cuts at: 0 239 251 650 917 2102 2378 2387 2399 Size: 239 12 399 267 1185 276 9 12 Cuts at: 2399 2464 2466 Size: 65 2 Fragments arranged by size: 1185 399 276 267 239 65 12 12 9 2 PvuII CAG'CTG Cuts at: 0 1119 2024 2466 Size: 1119 905 442 Fragments arranged by size: 1119 905 442 SacI G_AGCT'C Cuts at: 0 164 1031 1480 2466 Size: 164 867 449 986 Fragments arranged by size: 986 867 449 164 SanDI GG'GwC_CC Cuts at: 0 1440 2466 Size: 1440 1026 SapI GCTCTTCn'nnn_ Cuts at: 0 1036 2263 2466 Size: 1036 1227 203 Fragments arranged by size: 1227 1036 203 SbfI CC_TGCA'GG Cuts at: 0 917 2378 2466 Size: 917 1461 88 Fragments arranged by size: 1461 917 88 ScaI AGT'ACT Cuts at: 0 1087 2466 Size: 1087 1379 SexAI A'CCwGG_T Cuts at: 0 797 2466 Size: 797 1669 SfcI C'TryA_G Cuts at: 0 235 247 646 913 2098 2374 2383 2395 Size: 235 12 399 267 1185 276 9 12 Cuts at: 2395 2460 2466 Size: 65 6 Fragments arranged by size: 1185 399 276 267 235 65 12 12 9 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2227 2466 Size: 2227 239 SmaI CCC'GGG Cuts at: 0 808 2466 Size: 808 1658 SmlI C'TyrA_G Cuts at: 0 540 1081 1132 1885 2466 Size: 540 541 51 753 581 Fragments arranged by size: 753 581 541 540 51 StyI C'CwwG_G Cuts at: 0 1238 1934 2466 Size: 1238 696 532 Fragments arranged by size: 1238 696 532 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 195 521 1390 2466 Size: 195 326 869 1076 Fragments arranged by size: 1076 869 326 195 TatI w'GTAC_w Cuts at: 0 1085 1983 2466 Size: 1085 898 483 Fragments arranged by size: 1085 898 483 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 120 152 2466 Size: 120 32 2314 Fragments arranged by size: 2314 120 32 Tth111I GACn'n_nGTC Cuts at: 0 1438 2466 Size: 1438 1028 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 526 1245 2028 2466 Size: 526 719 783 438 Fragments arranged by size: 783 719 526 438 XmaI C'CCGG_G Cuts at: 0 806 2466 Size: 806 1660 Enzymes that do cut: AccI AcuI AfeI AflIII AleI AlfI AloI AlwNI AvaI BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SanDI SapI SbfI ScaI SexAI SfcI SfiI SmaI SmlI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglII BmtI BsaI BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI