################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## Acc65I AccIII AclI AcsI AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BglII BlnI BsaAI BsaBI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT14I EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 526 GTCGAC GT^[AC][GT]AC AflII 715 CTTAAG C^TTAAG AflIII 791 ACATGT A^C[GA][CT]GT AlwNI 1165 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 819 CCTCAGG CC^T[ACGT]AGG ApaI 59 GGGCCC GGGCC^C AvaI 63 CCCGGG C^[CT]CG[GA]G BbeI 488 GGCGCC GGCGC^C BbsI 1329 GAAGAC GAAGAC(2/6) BbvCI 874 GCTGAGG CCTCAGC(-5/-2) BclI 1340 TGATCA T^GATCA BfaI 658 CTAG C^TAG BfrI 715 CTTAAG C^TTAAG BglI 1069 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1329 GAAGAC GAAGAC(2/6) BsaI 844 GAGACC GGTCTC(1/5) BsaWI 1214 ACCGGA [AT]^CCGG[AT] BsoBI 63 CCCGGG C^[CT]CG[GA]G BspLU11I 791 ACATGT A^CATGT BsrDI 1098 CATTGC GCAATG(2/0) BstAPI 1398 GCATGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 212 CCAGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 819 CCTCAGG CC^T[ACGT]AGG DsaI 106 CCGCGG C^C[GA][CT]GG EarI 730 GAAGAG CTCTTC(1/4) Eco81I 819 CCTCAGG CC^T[ACGT]AGG EcoRV 826 GATATC GAT^ATC FbaI 1340 TGATCA T^GATCA HincII 526 GTCGAC GT[CT]^[GA]AC HindII 526 GTCGAC GT[CT]^[GA]AC KasI 488 GGCGCC G^GCGCC Ksp632I 730 GAAGAG CTCTTC(1/4) KspI 106 CCGCGG CCGC^GG MaeI 658 CTAG C^TAG MaeIII 145 GTGAC ^GT[ACGT]AC MseI 716 TTAA T^TAA NaeI 215 GCCGGC GCC^GGC NarI 488 GGCGCC GG^CGCC NgoMIV 215 GCCGGC G^CCGGC NspI 791 ACATGT [GA]CATG^[CT] PpuMI 1166 AGGACCT [GA]G^G[AT]CC[CT] SacII 106 CCGCGG CCGC^GG SalI 526 GTCGAC G^TCGAC SexAI 161 ACCAGGT A^CC[AT]GGT SfoI 488 GGCGCC GGC^GCC SmaI 63 CCCGGG CCC^GGG StuI 8 AGGCCT AGG^CCT Tru9I 716 TTAA T^TAA Tsp45I 145 GTGAC ^GT[GC]AC XcmI 317 CCAACGAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 63 CCCGGG C^CCGGG XmaI 63 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AatII 147 GACGTC GACGT^C 529 GACGTC BanI 488 GGCGCC G^G[CT][GA]CC 1124 GGCACC BlpI 87 GCTGAGC GC^T[ACGT]AGC 754 GCTCAGC Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC 754 GCTCAGC BsmAI 844 GAGAC GTCTC(1/5) 1354 GAGAC BsmFI 223 GGGAC GGGAC(10/14) 150 GTCCC BsrBI 73 CCGCTC CCGCTC(-3/-3) 635 GAGCGG BsrFI 215 GCCGGC [GA]^CCGG[CT] 422 ACCGGC BstYI 737 AGATCC [GA]^GATC[CT] 980 AGATCC CelII 87 GCTGAGC GC^T[ACGT]AGC 754 GCTCAGC Cfr10I 215 GCCGGC [GA]^CCGG[CT] 422 ACCGGC EaeI 278 TGGCCG [CT]^GGCC[GA] 452 TGGCCG HaeII 402 GGCGCT [GA]GCGC^[CT] 488 GGCGCC MflI 737 AGATCC [GA]^GATC[CT] 980 AGATCC SfaNI 646 GATGC GCATC(5/9) 1044 GATGC XhoII 737 AGATCC [GA]^GATC[CT] 980 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI RsaI SacI Sau3AI Sau96I ScrFI SfcI SmlI TaqI TfiI TseI Tsp509I TspRI TthHB8I