(Circular) (Six-base) MAPSORT of: pFN21AE4452.seq Check: 4153 from: 1 to: 6115 pFN21AE4452 6115 bp With 182 enzymes: SgfI * March 28, 2012 13:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3008 3008 Size: 6115 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2156 2538 3925 278 Size: 53 83 186 936 620 382 1387 2468 Fragments arranged by size: 2468 1387 936 620 382 186 83 53 AccI GT'mk_AC Cuts at: 1937 2532 3458 1937 Size: 595 926 4594 Fragments arranged by size: 4594 926 595 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2750 2895 4178 5562 5830 1632 Size: 1118 145 1283 1384 268 1917 Fragments arranged by size: 1917 1384 1283 1118 268 145 AflII C'TTAA_G Cuts at: 820 1017 2720 820 Size: 197 1703 4215 Fragments arranged by size: 4215 1703 197 AflIII A'CryG_T Cuts at: 1203 2796 5014 5991 1203 Size: 1593 2218 977 1327 Fragments arranged by size: 2218 1593 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6115 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2699 2731 2763 2795 2699 Size: 32 32 32 6019 Fragments arranged by size: 6019 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3175 3858 5430 3175 Size: 683 1572 3860 Fragments arranged by size: 3860 1572 683 ApaI G_GGCC'C Cuts at: 2068 2068 Size: 6115 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 3424 3650 3802 5911 1493 Size: 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2068 3440 1870 Size: 73 125 1372 4545 Fragments arranged by size: 4545 1372 125 73 BamHI G'GATC_C Cuts at: 3445 3812 3445 Size: 367 5748 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2493 3129 3436 4891 618 Size: 325 217 627 706 636 307 1455 1842 Fragments arranged by size: 1842 1455 706 636 627 325 307 217 BanII G_rGCy'C Cuts at: 1391 2068 2099 2138 2349 2466 2842 3434 1391 Size: 677 31 39 211 117 376 592 4072 Fragments arranged by size: 4072 677 592 376 211 117 39 31 BbeI G_GCGC'C Cuts at: 2497 2497 Size: 6115 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3341 928 Size: 971 1442 3702 Fragments arranged by size: 3702 1442 971 BbvCI CC'TCA_GC Cuts at: 2880 2880 Size: 6115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4306 4340 1903 Size: 34 2369 34 3678 Fragments arranged by size: 3678 2369 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 3345 1548 Size: 1797 4318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3080 4726 136 Size: 107 122 71 631 605 1408 1646 1525 Fragments arranged by size: 1646 1525 1408 631 605 122 107 71 BglII A'GATC_T Cuts at: 6110 6110 Size: 6115 BlpI GC'TnA_GC Cuts at: 2093 2760 3532 2093 Size: 667 772 4676 Fragments arranged by size: 4676 772 667 Bme1580I G_kGCm'C Cuts at: 1790 2068 3026 4176 5332 1790 Size: 278 958 1150 1156 2573 Fragments arranged by size: 2573 1156 1150 958 278 BmgBI CAC'GTC Cuts at: 1318 2181 2647 1318 Size: 863 466 4786 Fragments arranged by size: 4786 863 466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3846 4804 442 Size: 416 53 83 399 24 2429 958 1753 Fragments arranged by size: 2429 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 2411 2699 3134 3251 4775 1239 Size: 886 286 288 435 117 1524 2579 Fragments arranged by size: 2579 1524 886 435 288 286 117 Bpu10I CC'TnA_GC Cuts at: 2880 3303 2880 Size: 423 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2417 2558 3002 3119 4240 5105 5403 2417 Size: 141 444 117 1121 865 298 3129 Fragments arranged by size: 3129 1121 865 444 298 141 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2843 4778 882 Size: 617 1344 1935 2219 Fragments arranged by size: 2219 1935 1344 617 BsaAI yAC'GTr Cuts at: 493 1204 3906 493 Size: 711 2702 2702 Fragments arranged by size: 2702 2702 711 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2153 2494 2535 2940 Size: 53 83 186 936 620 341 41 405 Cuts at: 2940 3012 3922 4304 4939 275 Size: 72 910 382 635 1451 Fragments arranged by size: 1451 936 910 635 620 405 382 341 186 83 72 53 41 BsaWI w'CCGG_w Cuts at: 3219 4548 4946 5220 5367 5778 3219 Size: 1329 398 274 147 411 3556 Fragments arranged by size: 3556 1329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2585 2615 2699 2729 1178 Size: 30 165 30 1182 30 84 30 4564 Fragments arranged by size: 4564 1182 165 84 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2236 2486 2489 2606 2720 2753 2897 Size: 942 108 250 3 117 114 33 144 Cuts at: 2897 3131 3254 1186 Size: 234 123 4047 Fragments arranged by size: 4047 942 250 234 144 123 117 114 108 33 3 BseYI C'CCAG_C Cuts at: 2224 5318 5814 2224 Size: 3094 496 2525 Fragments arranged by size: 3094 2525 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5542 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3565 4326 5354 1540 Size: 275 186 1564 761 1028 2301 Fragments arranged by size: 2301 1564 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2099 2349 2466 2511 3414 4176 4261 5332 2099 Size: 250 117 45 903 762 85 1071 2882 Fragments arranged by size: 2882 1071 903 762 250 117 85 45 BsmI GAATG_Cn' Cuts at: 3626 3719 3626 Size: 93 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2099 2138 2349 2466 2511 2842 Size: 399 278 31 39 211 117 45 331 Cuts at: 2842 3026 3414 3434 4176 4261 5332 1391 Size: 184 388 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 388 331 278 211 184 117 85 45 39 31 20 BspEI T'CCGG_A Cuts at: 5778 5778 Size: 6115 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2559 2571 2767 3008 3183 3470 844 Size: 1715 12 196 241 175 287 3489 Fragments arranged by size: 3489 1715 287 241 196 175 12 BsrBI CCG'CTC Cuts at: 1394 1823 2080 2642 4002 1394 Size: 429 257 562 1360 3507 Fragments arranged by size: 3507 1360 562 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 3102 4610 4784 65 Size: 1183 1854 1508 174 1396 Fragments arranged by size: 1854 1508 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2220 2427 4759 4946 1700 Size: 110 410 207 2332 187 2869 Fragments arranged by size: 2869 2332 410 207 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 2521 2523 2525 1925 Size: 596 2 2 5515 Fragments arranged by size: 5515 596 2 2 BssSI C'ACGA_G Cuts at: 4175 5187 4175 Size: 1012 5103 BstAPI GCAn_nnn'nTGC Cuts at: 794 3409 794 Size: 2615 3500 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2224 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2742 2985 3445 3812 4197 4214 5666 5752 Size: 770 243 460 367 385 17 1452 86 Cuts at: 5752 5764 6110 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 770 460 385 367 346 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2825 2825 Size: 6115 BtgI C'CryG_G Cuts at: 513 1065 2111 513 Size: 552 1046 4517 Fragments arranged by size: 4517 1046 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2839 487 Size: 683 56 1613 3763 Fragments arranged by size: 3763 1613 683 56 BtsI GCAGTG_nn' Cuts at: 2814 3631 4425 4445 2814 Size: 817 794 20 4484 Fragments arranged by size: 4484 817 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2457 3562 Size: 54 1425 50 275 18 453 174 1105 Cuts at: 3562 4451 6080 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1105 889 453 275 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3562 1537 Size: 275 1750 4090 Fragments arranged by size: 4090 1750 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2730 4045 1353 Size: 1377 1315 3423 Fragments arranged by size: 3423 1377 1315 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4702 5076 5222 387 Size: 1064 138 3113 374 146 1280 Fragments arranged by size: 3113 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2097 2347 2464 2097 Size: 250 117 5748 Fragments arranged by size: 5748 250 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2411 2699 2750 2895 3134 3251 Size: 393 493 286 288 51 145 239 117 Cuts at: 3251 4178 4775 5562 5830 1239 Size: 927 597 787 268 1524 Fragments arranged by size: 1524 927 787 597 493 393 288 286 268 239 145 117 51 EcoNI CCTnn'n_nnAGG Cuts at: 2915 2948 3116 2915 Size: 33 168 5914 Fragments arranged by size: 5914 168 33 EcoO109I rG'GnC_Cy Cuts at: 2064 2065 2197 3172 2064 Size: 1 132 975 5007 Fragments arranged by size: 5007 975 132 1 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 EcoRV GAT'ATC Cuts at: 2833 2833 Size: 6115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 6115 FspI TGC'GCA Cuts at: 4621 4621 Size: 6115 HaeII r_GCGC'y Cuts at: 1678 2411 2497 5262 1678 Size: 733 86 2765 2531 Fragments arranged by size: 2765 2531 733 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2970 3002 4756 4788 4830 Size: 32 135 32 1398 32 1754 32 42 Cuts at: 4830 4862 1373 Size: 32 2626 Fragments arranged by size: 2626 1754 1398 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2533 3459 3705 669 Size: 988 281 595 926 246 3079 Fragments arranged by size: 3079 988 926 595 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6115 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KasI G'GCGC_C Cuts at: 2493 2493 Size: 6115 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5229 5413 1305 Size: 3924 184 2007 Fragments arranged by size: 3924 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4293 Fragments arranged by size: 4293 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4073 4432 4591 518 Size: 648 2907 359 159 2042 Fragments arranged by size: 2907 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2113 2569 3531 4208 5356 5601 1606 Size: 486 21 456 962 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 677 486 456 245 21 NaeI GCC'GGC Cuts at: 2222 2222 Size: 6115 NarI GG'CG_CC Cuts at: 2494 2494 Size: 6115 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5563 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NgoMIV G'CCGG_C Cuts at: 2220 2220 Size: 6115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 2800 3473 5018 1132 Size: 97 1571 673 1545 2229 Fragments arranged by size: 2229 1571 1545 673 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6115 PciI A'CATG_T Cuts at: 2796 5014 2796 Size: 2218 3897 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6115 PfoI T'CCnGG_A Cuts at: 2388 2388 Size: 6115 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2699 2731 4184 4216 5697 5729 2699 Size: 32 1453 32 1481 32 3085 Fragments arranged by size: 3085 1481 1453 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3172 3172 Size: 6115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspOMI G'GGCC_C Cuts at: 2064 2064 Size: 6115 PstI C_TGCA'G Cuts at: 830 2343 2568 2589 3467 830 Size: 1513 225 21 878 3478 Fragments arranged by size: 3478 1513 878 225 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 SacI G_AGCT'C Cuts at: 2099 2349 2466 2099 Size: 250 117 5748 Fragments arranged by size: 5748 250 117 SacII CC_GC'GG Cuts at: 2114 2114 Size: 6115 SalI G'TCGA_C Cuts at: 1936 2531 3457 1936 Size: 595 926 4594 Fragments arranged by size: 4594 926 595 SbfI CC_TGCA'GG Cuts at: 3467 3467 Size: 6115 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SexAI A'CCwGG_T Cuts at: 2166 2166 Size: 6115 SfcI C'TryA_G Cuts at: 826 1045 2339 2564 2585 3463 4598 5279 5470 Size: 219 1294 225 21 878 1135 681 191 Cuts at: 5470 826 Size: 1471 Fragments arranged by size: 1471 1294 1135 878 681 225 219 191 21 SfoI GGC'GCC Cuts at: 2495 2495 Size: 6115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6115 SmaI CCC'GGG Cuts at: 1872 2070 3442 1872 Size: 198 1372 4545 Fragments arranged by size: 4545 1372 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 2432 2573 2720 3017 3134 4219 Size: 197 926 489 141 147 297 117 1085 Cuts at: 4219 5120 5382 820 Size: 901 262 1553 Fragments arranged by size: 1553 1085 926 901 489 297 262 197 147 141 117 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 3473 1132 Size: 2341 3774 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 2015 1517 Size: 498 5617 StyI C'CwwG_G Cuts at: 513 1065 3554 513 Size: 552 2489 3074 Fragments arranged by size: 3074 2489 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3081 4142 4159 4312 4497 4942 325 Size: 1255 1501 1061 17 153 185 445 1498 Fragments arranged by size: 1501 1498 1255 1061 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4361 6073 96 Size: 274 80 33 51 1443 2384 1712 138 Fragments arranged by size: 2384 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6075 6107 6075 Size: 32 6083 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 2329 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 2068 3440 1870 Size: 198 1372 4545 Fragments arranged by size: 4545 1372 198 XmnI GAAnn'nnTTC Cuts at: 4244 4244 Size: 6115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2154 2536 3923 276 Size: 53 83 186 936 620 382 1387 2468 Fragments arranged by size: 2468 1387 936 620 382 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI