(Linear) (Six-base) MAPSORT of: KIEE4452.seq Check: 2273 from: 1 to: 1410 KIEE4452.orf With 182 enzymes: SgfI * March 22, 2012 18:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1003 1410 Size: 1003 407 AatII G_ACGT'C Cuts at: 0 151 533 1410 Size: 151 382 877 Fragments arranged by size: 877 382 151 AccI GT'mk_AC Cuts at: 0 527 1410 Size: 527 883 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 745 890 1410 Size: 745 145 520 Fragments arranged by size: 745 520 145 AflII C'TTAA_G Cuts at: 0 715 1410 Size: 715 695 AflIII A'CryG_T Cuts at: 0 791 1410 Size: 791 619 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 694 726 758 790 1410 Size: 694 32 32 32 620 Fragments arranged by size: 694 620 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1170 1410 Size: 1170 240 ApaI G_GGCC'C Cuts at: 0 63 1410 Size: 63 1347 AvaI C'yCGr_G Cuts at: 0 63 1410 Size: 63 1347 BanI G'GyrC_C Cuts at: 0 488 1124 1410 Size: 488 636 286 Fragments arranged by size: 636 488 286 BanII G_rGCy'C Cuts at: 0 63 94 133 344 461 837 1410 Size: 63 31 39 211 117 376 573 Fragments arranged by size: 573 376 211 117 63 39 31 BbeI G_GCGC'C Cuts at: 0 492 1410 Size: 492 918 BbsI GAAGACnn'nnnn_ Cuts at: 0 1336 1410 Size: 1336 74 BbvCI CC'TCA_GC Cuts at: 0 875 1410 Size: 875 535 BclI T'GATC_A Cuts at: 0 1340 1410 Size: 1340 70 BglI GCCn_nnn'nGGC Cuts at: 0 1075 1410 Size: 1075 335 BlpI GC'TnA_GC Cuts at: 0 88 755 1410 Size: 88 667 655 Fragments arranged by size: 667 655 88 Bme1580I G_kGCm'C Cuts at: 0 63 1021 1410 Size: 63 958 389 Fragments arranged by size: 958 389 63 BmgBI CAC'GTC Cuts at: 0 176 642 1410 Size: 176 466 768 Fragments arranged by size: 768 466 176 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 406 694 1129 1246 1410 Size: 120 286 288 435 117 164 Fragments arranged by size: 435 288 286 164 120 117 Bpu10I CC'TnA_GC Cuts at: 0 875 1298 1410 Size: 875 423 112 Fragments arranged by size: 875 423 112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 553 997 1114 1410 Size: 412 141 444 117 296 Fragments arranged by size: 444 412 296 141 117 BsaI GGTCTCn'nnnn_ Cuts at: 0 838 1410 Size: 838 572 BsaHI Gr'CG_yC Cuts at: 0 148 489 530 935 1007 1410 Size: 148 341 41 405 72 403 Fragments arranged by size: 405 403 341 148 72 41 BsaWI w'CCGG_w Cuts at: 0 1214 1410 Size: 1214 196 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 580 610 694 724 1410 Size: 580 30 84 30 686 Fragments arranged by size: 686 580 84 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 123 231 481 484 601 715 748 892 Size: 123 108 250 3 117 114 33 144 Cuts at: 892 1126 1249 1410 Size: 234 123 161 Fragments arranged by size: 250 234 161 144 123 123 117 114 108 33 3 BseYI C'CCAG_C Cuts at: 0 219 1410 Size: 219 1191 BsiHKAI G_wGCw'C Cuts at: 0 94 344 461 506 1409 1410 Size: 94 250 117 45 903 1 Fragments arranged by size: 903 250 117 94 45 1 Bsp1286I G_dGCh'C Cuts at: 0 63 94 133 344 461 506 837 1021 Size: 63 31 39 211 117 45 331 184 Cuts at: 1021 1409 1410 Size: 388 1 Fragments arranged by size: 388 331 211 184 117 63 45 39 31 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 554 566 762 1003 1178 1410 Size: 554 12 196 241 175 232 Fragments arranged by size: 554 241 232 196 175 12 BsrBI CCG'CTC Cuts at: 0 75 637 1410 Size: 75 562 773 Fragments arranged by size: 773 562 75 BsrDI GCAATG_nn' Cuts at: 0 1097 1410 Size: 1097 313 BsrFI r'CCGG_y Cuts at: 0 215 422 1410 Size: 215 207 988 Fragments arranged by size: 988 215 207 BssHII G'CGCG_C Cuts at: 0 516 518 520 1410 Size: 516 2 2 890 Fragments arranged by size: 890 516 2 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1404 1410 Size: 1404 6 BstXI CCAn_nnnn'nTGG Cuts at: 0 219 1410 Size: 219 1191 BstYI r'GATC_y Cuts at: 0 737 980 1410 Size: 737 243 430 Fragments arranged by size: 737 430 243 Bsu36I CC'TnA_GG Cuts at: 0 820 1410 Size: 820 590 BtgI C'CryG_G Cuts at: 0 106 1410 Size: 106 1304 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 834 1410 Size: 834 576 BtsI GCAGTG_nn' Cuts at: 0 809 1410 Size: 809 601 EaeI y'GGCC_r Cuts at: 0 278 452 1410 Size: 278 174 958 Fragments arranged by size: 958 278 174 EarI CTCTTCn'nnn_ Cuts at: 0 725 1410 Size: 725 685 EcoICRI GAG'CTC Cuts at: 0 92 342 459 1410 Size: 92 250 117 951 Fragments arranged by size: 951 250 117 92 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 406 694 745 890 1129 1246 1410 Size: 120 286 288 51 145 239 117 164 Fragments arranged by size: 288 286 239 164 145 120 117 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 910 943 1111 1410 Size: 910 33 168 299 Fragments arranged by size: 910 299 168 33 EcoO109I rG'GnC_Cy Cuts at: 0 59 60 192 1167 1410 Size: 59 1 132 975 243 Fragments arranged by size: 975 243 132 59 1 EcoRV GAT'ATC Cuts at: 0 828 1410 Size: 828 582 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 396 1410 Size: 396 1014 HaeII r_GCGC'y Cuts at: 0 406 492 1410 Size: 406 86 918 Fragments arranged by size: 918 406 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 965 997 1410 Size: 965 32 413 Fragments arranged by size: 965 413 32 HincII GTy'rAC Cuts at: 0 528 1410 Size: 528 882 KasI G'GCGC_C Cuts at: 0 488 1410 Size: 488 922 MspA1I CmG'CkG Cuts at: 0 87 108 564 1410 Size: 87 21 456 846 Fragments arranged by size: 846 456 87 21 NaeI GCC'GGC Cuts at: 0 217 1410 Size: 217 1193 NarI GG'CG_CC Cuts at: 0 489 1410 Size: 489 921 NgoMIV G'CCGG_C Cuts at: 0 215 1410 Size: 215 1195 NspI r_CATG'y Cuts at: 0 795 1410 Size: 795 615 PciI A'CATG_T Cuts at: 0 791 1410 Size: 791 619 PfoI T'CCnGG_A Cuts at: 0 383 1410 Size: 383 1027 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 694 726 1410 Size: 694 32 684 Fragments arranged by size: 694 684 32 PpuMI rG'GwC_Cy Cuts at: 0 1167 1410 Size: 1167 243 PspOMI G'GGCC_C Cuts at: 0 59 1410 Size: 59 1351 PstI C_TGCA'G Cuts at: 0 338 563 584 1410 Size: 338 225 21 826 Fragments arranged by size: 826 338 225 21 SacI G_AGCT'C Cuts at: 0 94 344 461 1410 Size: 94 250 117 949 Fragments arranged by size: 949 250 117 94 SacII CC_GC'GG Cuts at: 0 109 1410 Size: 109 1301 SalI G'TCGA_C Cuts at: 0 526 1410 Size: 526 884 SexAI A'CCwGG_T Cuts at: 0 161 1410 Size: 161 1249 SfcI C'TryA_G Cuts at: 0 334 559 580 1410 Size: 334 225 21 830 Fragments arranged by size: 830 334 225 21 SfoI GGC'GCC Cuts at: 0 490 1410 Size: 490 920 SmaI CCC'GGG Cuts at: 0 65 1410 Size: 65 1345 SmlI C'TyrA_G Cuts at: 0 427 568 715 1012 1129 1410 Size: 427 141 147 297 117 281 Fragments arranged by size: 427 297 281 147 141 117 StuI AGG'CCT Cuts at: 0 10 1410 Size: 10 1400 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1076 1410 Size: 1076 334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 324 1410 Size: 324 1086 XmaI C'CCGG_G Cuts at: 0 63 1410 Size: 63 1347 ZraI GAC'GTC Cuts at: 0 149 531 1410 Size: 149 382 879 Fragments arranged by size: 879 382 149 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PpiI PpuMI PspOMI PstI SacI SacII SalI SexAI SfcI SfoI SmaI SmlI StuI TaqII XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BglII BmrI BmtI BplI BsaAI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI