(Circular) (Six-base) MAPSORT of: pFN21AE0972.seq Check: 742 from: 1 to: 6265 pFN21AE0972 6265 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2895 4075 278 Size: 53 83 186 936 1359 1180 2468 Fragments arranged by size: 2468 1359 1180 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 2633 3088 3299 4328 5712 5980 1632 Size: 887 114 455 211 1029 1384 268 1917 Fragments arranged by size: 1917 1384 1029 887 455 268 211 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2383 1184 Size: 1199 5066 AlwNI CAG_nnn'CTG Cuts at: 2575 4008 5580 2575 Size: 1433 1572 3260 Fragments arranged by size: 3260 1572 1433 ApaI G_GGCC'C Cuts at: 2829 2829 Size: 6265 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2307 2530 2906 3074 3299 3590 Size: 73 97 267 223 376 168 225 291 Cuts at: 3590 1870 Size: 4545 Fragments arranged by size: 4545 376 291 267 225 223 168 97 73 BamHI G'GATC_C Cuts at: 2035 2780 3595 3962 2035 Size: 745 815 367 4338 Fragments arranged by size: 4338 815 745 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2414 3316 3586 5041 618 Size: 325 217 627 627 902 270 1455 1842 Fragments arranged by size: 1842 1455 902 627 627 325 270 217 BanII G_rGCy'C Cuts at: 1391 2829 3225 3264 3366 3513 3584 1391 Size: 1438 396 39 102 147 71 4072 Fragments arranged by size: 4072 1438 396 147 102 71 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2460 928 Size: 971 561 4733 Fragments arranged by size: 4733 971 561 BbvCI CC'TCA_GC Cuts at: 2670 3479 2670 Size: 809 5456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3077 4876 136 Size: 107 122 71 631 605 1405 1799 1525 Fragments arranged by size: 1799 1525 1405 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3033 3682 3033 Size: 649 5616 Bme1580I G_kGCm'C Cuts at: 1790 2829 2844 2982 3173 4326 5482 1790 Size: 1039 15 138 191 1153 1156 2573 Fragments arranged by size: 2573 1156 1153 1039 191 138 15 BmgBI CAC'GTC Cuts at: 1318 2519 1318 Size: 1201 5064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2603 3531 4925 1239 Size: 1037 327 928 1394 2579 Fragments arranged by size: 2579 1394 1037 928 327 Bpu10I CC'TnA_GC Cuts at: 2670 3479 2670 Size: 809 5456 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3248 4390 5255 5553 3248 Size: 1142 865 298 3960 Fragments arranged by size: 3960 1142 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2344 2629 2787 2983 4056 493 Size: 711 1140 285 158 196 1073 2702 Fragments arranged by size: 2702 1140 1073 711 285 196 158 BsaBI GATnn'nnATC Cuts at: 2606 6259 2606 Size: 3653 2612 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2892 3003 3138 4072 Size: 53 83 186 936 1359 111 135 934 Cuts at: 4072 4454 5089 275 Size: 382 635 1451 Fragments arranged by size: 1451 1359 936 934 635 382 186 135 111 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 1178 Size: 30 165 30 1173 30 4837 Fragments arranged by size: 4837 1173 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2879 3272 3374 3392 3395 3425 3428 Size: 919 774 393 102 18 3 30 3 Cuts at: 3428 1186 Size: 4023 Fragments arranged by size: 4023 919 774 393 102 30 18 3 3 BseYI C'CCAG_C Cuts at: 2191 2267 2665 3058 3443 5468 5964 2191 Size: 76 398 393 385 2025 496 2492 Fragments arranged by size: 2492 2025 496 398 393 385 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 1163 Size: 573 657 5035 Fragments arranged by size: 5035 657 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3264 3387 4326 4411 5482 3264 Size: 123 939 85 1071 4047 Fragments arranged by size: 4047 1071 939 123 85 BsmI GAATG_Cn' Cuts at: 3062 3776 3869 3062 Size: 714 93 5458 Fragments arranged by size: 5458 714 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3296 3296 Size: 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2829 2844 2982 3173 3225 3264 3366 Size: 399 1039 15 138 191 52 39 102 Cuts at: 3366 3387 3513 3584 4326 4411 5482 1391 Size: 21 126 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 1039 742 399 191 138 126 102 85 71 52 39 21 15 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2892 2967 3620 844 Size: 2048 75 653 3489 Fragments arranged by size: 3489 2048 653 75 BsrBI CCG'CTC Cuts at: 1394 1823 2236 2587 4152 1394 Size: 429 413 351 1565 3507 Fragments arranged by size: 3507 1565 429 413 351 BsrDI GCAATG_nn' Cuts at: 65 1248 2475 4760 4934 65 Size: 1183 1227 2285 174 1396 Fragments arranged by size: 2285 1396 1227 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 2795 1925 Size: 870 5395 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 2575 794 Size: 1781 4484 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 3343 1184 Size: 2159 4106 BstYI r'GATC_y Cuts at: 1972 2035 2780 3595 3962 4347 4364 5816 5902 Size: 63 745 815 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 815 745 385 367 346 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 3237 3237 Size: 6265 BtgI C'CryG_G Cuts at: 513 1065 2004 2141 2280 3225 3453 513 Size: 552 939 137 139 945 228 3325 Fragments arranged by size: 3325 945 939 552 228 139 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3385 3781 4575 4595 2917 Size: 468 396 794 20 4587 Fragments arranged by size: 4587 794 468 396 20 ClaI AT'CG_AT Cuts at: 1554 2073 1554 Size: 519 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DraIII CAC_nnn'GTG Cuts at: 2344 2344 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3002 5272 809 Size: 507 1686 2270 1802 Fragments arranged by size: 2270 1802 1686 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 2346 3094 Size: 54 1425 50 275 18 407 109 748 Cuts at: 3094 3712 4601 6230 8 Size: 618 889 1629 43 Fragments arranged by size: 1629 1425 889 748 618 407 275 109 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3522 4195 1353 Size: 2169 673 3423 Fragments arranged by size: 3423 2169 673 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2332 4852 5226 5372 387 Size: 1064 138 743 2520 374 146 1280 Fragments arranged by size: 2520 1280 1064 743 374 146 138 EcoICRI GAG'CTC Cuts at: 3262 3262 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2519 2603 2633 3088 3299 3531 Size: 393 644 243 84 30 455 211 232 Cuts at: 3531 4328 4925 5712 5980 1239 Size: 797 597 787 268 1524 Fragments arranged by size: 1524 797 787 644 597 455 393 268 243 232 211 84 30 EcoNI CCTnn'n_nnAGG Cuts at: 2317 2819 2317 Size: 502 5763 EcoO109I rG'GnC_Cy Cuts at: 2285 2538 2653 2825 3268 2285 Size: 253 115 172 443 5282 Fragments arranged by size: 5282 443 253 172 115 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3508 3508 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2765 2797 4906 4938 4980 Size: 32 135 32 1193 32 2109 32 42 Cuts at: 4980 5012 1373 Size: 32 2626 Fragments arranged by size: 2626 2109 1193 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 2219 748 Size: 1471 4794 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 5379 5563 1305 Size: 1674 2400 184 2007 Fragments arranged by size: 2400 2007 1674 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2348 10 Size: 54 1425 343 516 3927 Fragments arranged by size: 3927 1425 516 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2631 2837 4223 4582 4741 518 Size: 648 909 556 206 1386 359 159 2042 Fragments arranged by size: 2042 1386 909 648 556 359 206 159 MspA1I CmG'CkG Cuts at: 1606 2021 2377 2572 2669 3037 3447 3681 4358 Size: 415 356 195 97 368 410 234 677 Cuts at: 4358 5506 5751 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 415 410 368 356 245 234 195 97 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4774 Fragments arranged by size: 4774 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3623 5168 1132 Size: 97 2394 1545 2229 Fragments arranged by size: 2394 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2551 1348 Size: 1203 5062 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 1108 Size: 1236 5029 PfoI T'CCnGG_A Cuts at: 2655 3547 2655 Size: 892 5373 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PmlI CAC'GTG Cuts at: 2344 2629 2787 2983 2344 Size: 285 158 196 5626 Fragments arranged by size: 5626 285 196 158 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3472 3504 4334 4366 5847 5879 3472 Size: 32 830 32 1481 32 3858 Fragments arranged by size: 3858 1481 830 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2538 2653 3268 2538 Size: 115 615 5535 Fragments arranged by size: 5535 615 115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2825 2825 Size: 6265 PspXI vC'TCGA_Gb Cuts at: 2906 2906 Size: 6265 PstI C_TGCA'G Cuts at: 830 2397 2697 3392 3617 830 Size: 1567 300 695 225 3478 Fragments arranged by size: 3478 1567 695 300 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2021 2377 2572 2669 3037 3447 2021 Size: 356 195 97 368 410 4839 Fragments arranged by size: 4839 410 368 356 195 97 SacI G_AGCT'C Cuts at: 3264 3264 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SanDI GG'GwC_CC Cuts at: 2538 2538 Size: 6265 SapI GCTCTTCn'nnn_ Cuts at: 3522 3522 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 2176 2912 4513 6225 2176 Size: 736 1601 1712 2216 Fragments arranged by size: 2216 1712 1601 736 SfcI C'TryA_G Cuts at: 826 1045 2228 2393 2693 3388 3613 4748 5429 Size: 219 1183 165 300 695 225 1135 681 Cuts at: 5429 5620 826 Size: 191 1471 Fragments arranged by size: 1471 1183 1135 695 681 300 225 219 191 165 SfiI GGCCn_nnn'nGGCC Cuts at: 3077 3077 Size: 6265 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 2309 3592 1872 Size: 437 1283 4545 Fragments arranged by size: 4545 1283 437 SmlI C'TyrA_G Cuts at: 820 1017 1943 2906 3263 4369 5270 5532 820 Size: 197 926 963 357 1106 901 262 1553 Fragments arranged by size: 1553 1106 963 926 901 357 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 3116 1517 Size: 1599 4666 StyI C'CwwG_G Cuts at: 513 1065 2004 3117 3338 3704 513 Size: 552 939 1113 221 366 3074 Fragments arranged by size: 3074 1113 939 552 366 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3331 4292 4309 4462 4647 5092 325 Size: 1255 1751 961 17 153 185 445 1498 Fragments arranged by size: 1751 1498 1255 961 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2174 2385 2910 Size: 274 80 33 51 1443 197 211 525 Cuts at: 2910 3147 4511 6223 96 Size: 237 1364 1712 138 Fragments arranged by size: 1712 1443 1364 525 274 237 211 197 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2962 3104 3460 2962 Size: 142 356 5767 Fragments arranged by size: 5767 356 142 XhoI C'TCGA_G Cuts at: 1943 2906 1943 Size: 963 5302 XmaI C'CCGG_G Cuts at: 1870 2307 3590 1870 Size: 437 1283 4545 Fragments arranged by size: 4545 1283 437 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2893 4073 276 Size: 53 83 186 936 1359 1180 2468 Fragments arranged by size: 2468 1359 1180 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII SexAI SfoI SrfI SwaI