(Circular) (Six-base) MAPSORT of: pFN21AE3930.seq Check: 1256 from: 1 to: 5908 pFN21AE3930 5908 bp With 182 enzymes: SgfI * March 28, 2012 11:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2227 2227 Size: 5908 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2716 2744 3971 5355 5623 1632 Size: 1084 28 1227 1384 268 1917 Fragments arranged by size: 1917 1384 1227 1084 268 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 4807 5784 1203 Size: 3604 977 1327 Fragments arranged by size: 3604 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2135 2324 2740 1184 Size: 951 189 416 4352 Fragments arranged by size: 4352 951 416 189 AlwNI CAG_nnn'CTG Cuts at: 2128 2266 3143 3651 5223 2128 Size: 138 877 508 1572 2813 Fragments arranged by size: 2813 1572 877 508 138 ApaI G_GGCC'C Cuts at: 2107 2220 2107 Size: 113 5795 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 3217 3443 3595 5704 1493 Size: 1724 226 152 2109 1697 Fragments arranged by size: 2109 1724 1697 226 152 AscI GG'CGCG_CC Cuts at: 2132 2132 Size: 5908 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2049 2606 2699 2864 3233 1870 Size: 73 84 22 557 93 165 369 4545 Fragments arranged by size: 4545 557 369 165 93 84 73 22 BamHI G'GATC_C Cuts at: 2970 3238 3605 2970 Size: 268 367 5273 Fragments arranged by size: 5273 367 268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2032 2167 2610 2654 Size: 325 217 627 236 9 135 443 44 Cuts at: 2654 2703 3229 4684 618 Size: 49 526 1455 1842 Fragments arranged by size: 1842 1455 627 526 443 325 236 217 135 49 44 9 BanII G_rGCy'C Cuts at: 1391 2013 2107 2196 2220 2631 3227 1391 Size: 622 94 89 24 411 596 4072 Fragments arranged by size: 4072 622 596 411 94 89 24 BbeI G_GCGC'C Cuts at: 2027 2658 2707 2027 Size: 631 49 5228 Fragments arranged by size: 5228 631 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4937 BbvCI CC'TCA_GC Cuts at: 3190 3190 Size: 5908 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3203 4519 136 Size: 107 122 71 631 605 1531 1316 1525 Fragments arranged by size: 1531 1525 1316 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 2472 3325 2472 Size: 853 5055 Bme1580I G_kGCm'C Cuts at: 1790 2107 2114 2220 2613 3969 5125 1790 Size: 317 7 106 393 1356 1156 2573 Fragments arranged by size: 2573 1356 1156 393 317 106 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3639 4597 442 Size: 416 53 83 399 24 2222 958 1753 Fragments arranged by size: 2222 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 3120 4568 1239 Size: 971 910 1448 2579 Fragments arranged by size: 2579 1448 971 910 Bpu10I CC'TnA_GC Cuts at: 3190 3190 Size: 5908 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 2783 4033 4898 5196 2336 Size: 447 1250 865 298 3048 Fragments arranged by size: 3048 1250 865 447 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2052 2417 2988 3071 4571 882 Size: 617 553 365 571 83 1500 2219 Fragments arranged by size: 2219 1500 617 571 553 365 83 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 5902 5902 Size: 5908 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 2655 2704 3715 Size: 53 83 186 936 491 631 49 1011 Cuts at: 3715 4097 4732 275 Size: 382 635 1451 Fragments arranged by size: 1451 1011 936 635 631 491 382 186 83 53 49 BsaWI w'CCGG_w Cuts at: 2886 4341 4739 5013 5160 5571 2886 Size: 1455 398 274 147 411 3223 Fragments arranged by size: 3223 1455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2072 2102 1178 Size: 30 165 30 669 30 4984 Fragments arranged by size: 4984 669 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2342 2756 3131 1186 Size: 1111 45 414 375 3963 Fragments arranged by size: 3963 1111 414 375 45 BseYI C'CCAG_C Cuts at: 2040 2505 3095 5111 5607 2040 Size: 465 590 2016 496 2341 Fragments arranged by size: 2341 2016 590 496 465 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3075 1163 Size: 573 1339 3996 Fragments arranged by size: 3996 1339 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2076 2160 2643 3358 4119 5147 Size: 275 186 75 84 483 715 761 1028 Cuts at: 5147 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 715 483 275 186 84 75 BsiHKAI G_wGCw'C Cuts at: 2196 3969 4054 5125 2196 Size: 1773 85 1071 2979 Fragments arranged by size: 2979 1773 1071 85 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2107 2114 2196 2220 2613 2631 Size: 399 223 94 7 82 24 393 18 Cuts at: 2631 3227 3969 4054 5125 1391 Size: 596 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 596 399 393 223 94 85 82 24 18 7 BspEI T'CCGG_A Cuts at: 2886 5571 2886 Size: 2685 3223 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2227 2412 3263 844 Size: 1383 185 851 3489 Fragments arranged by size: 3489 1383 851 185 BsrBI CCG'CTC Cuts at: 1394 1823 2767 3795 1394 Size: 429 944 1028 3507 Fragments arranged by size: 3507 1028 944 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4403 4577 65 Size: 1183 3155 174 1396 Fragments arranged by size: 3155 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2251 2645 4552 4739 1700 Size: 110 441 394 1907 187 2869 Fragments arranged by size: 2869 1907 441 394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 2132 2311 2332 2334 2512 1925 Size: 207 179 21 2 178 5321 Fragments arranged by size: 5321 207 179 178 21 2 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 794 Size: 1472 4436 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstXI CCAn_nnnn'nTGG Cuts at: 2549 2878 2549 Size: 329 5579 BstYI r'GATC_y Cuts at: 1972 2125 2754 2970 3238 3605 3990 4007 5459 Size: 153 629 216 268 367 385 17 1452 Cuts at: 5459 5545 5557 5903 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 629 385 367 346 268 216 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2670 2670 Size: 5908 BtgI C'CryG_G Cuts at: 513 1065 2004 2058 2076 2211 2966 513 Size: 552 939 54 18 135 755 3455 Fragments arranged by size: 3455 939 755 552 135 54 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 2729 2953 3424 4218 4238 2729 Size: 224 471 794 20 4399 Fragments arranged by size: 4399 794 471 224 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2846 4915 809 Size: 507 1530 2069 1802 Fragments arranged by size: 2069 1802 1530 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2157 2416 Size: 54 1425 50 275 18 243 84 259 Cuts at: 2416 2493 3355 4244 5873 8 Size: 77 862 889 1629 43 Fragments arranged by size: 1629 1425 889 862 275 259 243 84 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2073 2157 3355 1537 Size: 275 261 84 1198 4090 Fragments arranged by size: 4090 1198 275 261 84 EarI CTCTTCn'nnn_ Cuts at: 1353 3108 3838 1353 Size: 1755 730 3423 Fragments arranged by size: 3423 1755 730 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2270 2696 3138 4495 4869 5015 Size: 1064 138 681 426 442 1357 374 146 Cuts at: 5015 387 Size: 1280 Fragments arranged by size: 1357 1280 1064 681 442 426 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 2194 Size: 5908 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2716 2744 3120 3971 4568 5355 Size: 393 578 506 28 376 851 597 787 Cuts at: 5355 5623 1239 Size: 268 1524 Fragments arranged by size: 1524 851 787 597 578 506 393 376 268 28 EcoNI CCTnn'n_nnAGG Cuts at: 2798 2798 Size: 5908 EcoO109I rG'GnC_Cy Cuts at: 2103 2104 2216 2674 2848 3186 2103 Size: 1 112 458 174 338 4825 Fragments arranged by size: 4825 458 338 174 112 1 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 2027 2189 2296 2455 2542 2658 2707 5055 Size: 349 162 107 159 87 116 49 2348 Cuts at: 5055 1678 Size: 2531 Fragments arranged by size: 2531 2348 349 162 159 116 107 87 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4549 4581 4623 4655 1373 Size: 32 135 32 2977 32 42 32 2626 Fragments arranged by size: 2977 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3252 3498 669 Size: 988 281 1314 246 3079 Fragments arranged by size: 3079 1314 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KasI G'GCGC_C Cuts at: 2023 2654 2703 2023 Size: 631 49 5228 Fragments arranged by size: 5228 631 49 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5022 5206 1305 Size: 3717 184 2007 Fragments arranged by size: 3717 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3866 4225 4384 518 Size: 648 1710 990 359 159 2042 Fragments arranged by size: 2042 1710 990 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2060 2078 2374 2446 2509 2824 2896 3324 Size: 454 18 296 72 63 315 72 428 Cuts at: 3324 4001 5149 5394 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 454 428 315 296 245 72 72 63 18 NaeI GCC'GGC Cuts at: 2253 2647 2253 Size: 394 5514 NarI GG'CG_CC Cuts at: 2024 2655 2704 2024 Size: 631 49 5228 Fragments arranged by size: 5228 631 49 NcoI C'CATG_G Cuts at: 513 1065 2004 2966 513 Size: 552 939 962 3455 Fragments arranged by size: 3455 962 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NgoMIV G'CCGG_C Cuts at: 2251 2645 2251 Size: 394 5514 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 3266 4811 1132 Size: 97 2037 1545 2229 Fragments arranged by size: 2229 2037 1545 97 PasI CC'CwG_GG Cuts at: 1348 2107 2660 1348 Size: 759 553 4596 Fragments arranged by size: 4596 759 553 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2674 2848 2674 Size: 174 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PspOMI G'GGCC_C Cuts at: 2103 2216 2103 Size: 113 5795 PspXI vC'TCGA_Gb Cuts at: 2864 2864 Size: 5908 PstI C_TGCA'G Cuts at: 830 2124 2238 2451 3260 830 Size: 1294 114 213 809 3478 Fragments arranged by size: 3478 1294 809 213 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2446 2509 2446 Size: 63 5845 RsrII CG'GwC_CG Cuts at: 2365 2365 Size: 5908 SacI G_AGCT'C Cuts at: 2196 2196 Size: 5908 SacII CC_GC'GG Cuts at: 2061 2079 2061 Size: 18 5890 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SanDI GG'GwC_CC Cuts at: 2848 2848 Size: 5908 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SfcI C'TryA_G Cuts at: 826 1045 2120 2234 2447 3256 4391 5072 5263 Size: 219 1075 114 213 809 1135 681 191 Cuts at: 5263 826 Size: 1471 Fragments arranged by size: 1471 1135 1075 809 681 219 213 191 114 SfoI GGC'GCC Cuts at: 2025 2656 2705 2025 Size: 631 49 5228 Fragments arranged by size: 5228 631 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 2645 1700 Size: 945 4963 SmaI CCC'GGG Cuts at: 1872 2029 2608 3235 1872 Size: 157 579 627 4545 Fragments arranged by size: 4545 627 579 157 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 2798 2864 4012 4913 5175 Size: 197 926 408 447 66 1148 901 262 Cuts at: 5175 820 Size: 1553 Fragments arranged by size: 1553 1148 926 901 447 408 262 197 66 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SrfI GCCC'GGGC Cuts at: 2608 2608 Size: 5908 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 2004 2966 3347 513 Size: 552 939 962 381 3074 Fragments arranged by size: 3074 962 939 552 381 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2382 2441 3109 3935 3952 4105 4290 Size: 1255 802 59 668 826 17 153 185 Cuts at: 4290 4735 325 Size: 445 1498 Fragments arranged by size: 1498 1255 826 802 668 445 185 153 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4154 5866 96 Size: 274 80 33 51 1443 2177 1712 138 Fragments arranged by size: 2177 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5868 5900 5868 Size: 32 5876 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5908 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 2864 1943 Size: 921 4987 XmaI C'CCGG_G Cuts at: 1870 2027 2606 3233 1870 Size: 157 579 627 4545 Fragments arranged by size: 4545 627 579 157 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI SapI SexAI SfiI SwaI XcmI