(Linear) (Six-base) MAPSORT of: KIEE3930.seq Check: 2977 from: 1 to: 1203 KIEE3930.orf With 182 enzymes: SgfI * March 22, 2012 16:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 222 1203 Size: 222 981 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 711 739 1203 Size: 711 28 464 Fragments arranged by size: 711 464 28 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 130 319 735 1203 Size: 130 189 416 468 Fragments arranged by size: 468 416 189 130 AlwNI CAG_nnn'CTG Cuts at: 0 123 261 1138 1203 Size: 123 138 877 65 Fragments arranged by size: 877 138 123 65 ApaI G_GGCC'C Cuts at: 0 102 215 1203 Size: 102 113 988 Fragments arranged by size: 988 113 102 AscI GG'CGCG_CC Cuts at: 0 127 1203 Size: 127 1076 AvaI C'yCGr_G Cuts at: 0 22 44 601 694 859 1203 Size: 22 22 557 93 165 344 Fragments arranged by size: 557 344 165 93 22 22 BamHI G'GATC_C Cuts at: 0 965 1203 Size: 965 238 BanI G'GyrC_C Cuts at: 0 18 27 162 605 649 698 1203 Size: 18 9 135 443 44 49 505 Fragments arranged by size: 505 443 135 49 44 18 9 BanII G_rGCy'C Cuts at: 0 8 102 191 215 626 1203 Size: 8 94 89 24 411 577 Fragments arranged by size: 577 411 94 89 24 8 BbeI G_GCGC'C Cuts at: 0 22 653 702 1203 Size: 22 631 49 501 Fragments arranged by size: 631 501 49 22 BbvCI CC'TCA_GC Cuts at: 0 1185 1203 Size: 1185 18 BglI GCCn_nnn'nGGC Cuts at: 0 1198 1203 Size: 1198 5 BlpI GC'TnA_GC Cuts at: 0 467 1203 Size: 467 736 Bme1580I G_kGCm'C Cuts at: 0 102 109 215 608 1203 Size: 102 7 106 393 595 Fragments arranged by size: 595 393 106 102 7 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 1115 1203 Size: 205 910 88 Fragments arranged by size: 910 205 88 Bpu10I CC'TnA_GC Cuts at: 0 1185 1203 Size: 1185 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 778 1203 Size: 331 447 425 Fragments arranged by size: 447 425 331 BsaI GGTCTCn'nnnn_ Cuts at: 0 47 412 983 1066 1203 Size: 47 365 571 83 137 Fragments arranged by size: 571 365 137 83 47 BsaHI Gr'CG_yC Cuts at: 0 19 650 699 1203 Size: 19 631 49 504 Fragments arranged by size: 631 504 49 19 BsaWI w'CCGG_w Cuts at: 0 881 1203 Size: 881 322 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 67 97 1203 Size: 67 30 1106 Fragments arranged by size: 1106 67 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 292 337 751 1126 1203 Size: 292 45 414 375 77 Fragments arranged by size: 414 375 292 77 45 BseYI C'CCAG_C Cuts at: 0 35 500 1090 1203 Size: 35 465 590 113 Fragments arranged by size: 590 465 113 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1070 1203 Size: 1070 133 BsiEI CG_ry'CG Cuts at: 0 71 155 638 1203 Size: 71 84 483 565 Fragments arranged by size: 565 483 84 71 BsiHKAI G_wGCw'C Cuts at: 0 191 1203 Size: 191 1012 Bsp1286I G_dGCh'C Cuts at: 0 8 102 109 191 215 608 626 1203 Size: 8 94 7 82 24 393 18 577 Fragments arranged by size: 577 393 94 82 24 18 8 7 BspEI T'CCGG_A Cuts at: 0 881 1203 Size: 881 322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 222 407 1203 Size: 222 185 796 Fragments arranged by size: 796 222 185 BsrBI CCG'CTC Cuts at: 0 762 1203 Size: 762 441 BsrFI r'CCGG_y Cuts at: 0 246 640 1203 Size: 246 394 563 Fragments arranged by size: 563 394 246 BssHII G'CGCG_C Cuts at: 0 127 306 327 329 507 1203 Size: 127 179 21 2 178 696 Fragments arranged by size: 696 179 178 127 21 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 261 1203 Size: 261 942 BstXI CCAn_nnnn'nTGG Cuts at: 0 544 873 1203 Size: 544 329 330 Fragments arranged by size: 544 330 329 BstYI r'GATC_y Cuts at: 0 120 749 965 1203 Size: 120 629 216 238 Fragments arranged by size: 629 238 216 120 Bsu36I CC'TnA_GG Cuts at: 0 665 1203 Size: 665 538 BtgI C'CryG_G Cuts at: 0 53 71 206 961 1203 Size: 53 18 135 755 242 Fragments arranged by size: 755 242 135 53 18 BtsI GCAGTG_nn' Cuts at: 0 724 948 1203 Size: 724 224 255 Fragments arranged by size: 724 255 224 DrdI GACnn_nn'nnGTC Cuts at: 0 841 1203 Size: 841 362 EaeI y'GGCC_r Cuts at: 0 68 152 411 488 1203 Size: 68 84 259 77 715 Fragments arranged by size: 715 259 84 77 68 EagI C'GGCC_G Cuts at: 0 68 152 1203 Size: 68 84 1051 Fragments arranged by size: 1051 84 68 EarI CTCTTCn'nnn_ Cuts at: 0 1103 1203 Size: 1103 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 265 691 1133 1203 Size: 265 426 442 70 Fragments arranged by size: 442 426 265 70 EcoICRI GAG'CTC Cuts at: 0 189 1203 Size: 189 1014 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 711 739 1115 1203 Size: 205 506 28 376 88 Fragments arranged by size: 506 376 205 88 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 793 1203 Size: 793 410 EcoO109I rG'GnC_Cy Cuts at: 0 98 99 211 669 843 1181 1203 Size: 98 1 112 458 174 338 22 Fragments arranged by size: 458 338 174 112 98 22 1 HaeII r_GCGC'y Cuts at: 0 22 184 291 450 537 653 702 1203 Size: 22 162 107 159 87 116 49 501 Fragments arranged by size: 501 162 159 116 107 87 49 22 KasI G'GCGC_C Cuts at: 0 18 649 698 1203 Size: 18 631 49 505 Fragments arranged by size: 631 505 49 18 MslI CAynn'nnrTG Cuts at: 0 871 1203 Size: 871 332 MspA1I CmG'CkG Cuts at: 0 55 73 369 441 504 819 891 1203 Size: 55 18 296 72 63 315 72 312 Fragments arranged by size: 315 312 296 72 72 63 55 18 NaeI GCC'GGC Cuts at: 0 248 642 1203 Size: 248 394 561 Fragments arranged by size: 561 394 248 NarI GG'CG_CC Cuts at: 0 19 650 699 1203 Size: 19 631 49 504 Fragments arranged by size: 631 504 49 19 NcoI C'CATG_G Cuts at: 0 961 1203 Size: 961 242 NgoMIV G'CCGG_C Cuts at: 0 246 640 1203 Size: 246 394 563 Fragments arranged by size: 563 394 246 PasI CC'CwG_GG Cuts at: 0 102 655 1203 Size: 102 553 548 Fragments arranged by size: 553 548 102 PpuMI rG'GwC_Cy Cuts at: 0 669 843 1203 Size: 669 174 360 Fragments arranged by size: 669 360 174 PspOMI G'GGCC_C Cuts at: 0 98 211 1203 Size: 98 113 992 Fragments arranged by size: 992 113 98 PspXI vC'TCGA_Gb Cuts at: 0 859 1203 Size: 859 344 PstI C_TGCA'G Cuts at: 0 119 233 446 1203 Size: 119 114 213 757 Fragments arranged by size: 757 213 119 114 PvuII CAG'CTG Cuts at: 0 441 504 1203 Size: 441 63 699 Fragments arranged by size: 699 441 63 RsrII CG'GwC_CG Cuts at: 0 360 1203 Size: 360 843 SacI G_AGCT'C Cuts at: 0 191 1203 Size: 191 1012 SacII CC_GC'GG Cuts at: 0 56 74 1203 Size: 56 18 1129 Fragments arranged by size: 1129 56 18 SanDI GG'GwC_CC Cuts at: 0 843 1203 Size: 843 360 SfcI C'TryA_G Cuts at: 0 115 229 442 1203 Size: 115 114 213 761 Fragments arranged by size: 761 213 115 114 SfoI GGC'GCC Cuts at: 0 20 651 700 1203 Size: 20 631 49 503 Fragments arranged by size: 631 503 49 20 SgrAI Cr'CCGG_yG Cuts at: 0 640 1203 Size: 640 563 SmaI CCC'GGG Cuts at: 0 24 603 1203 Size: 24 579 600 Fragments arranged by size: 600 579 24 SmlI C'TyrA_G Cuts at: 0 346 793 859 1203 Size: 346 447 66 344 Fragments arranged by size: 447 346 344 66 SrfI GCCC'GGGC Cuts at: 0 603 1203 Size: 603 600 StyI C'CwwG_G Cuts at: 0 961 1203 Size: 961 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 377 436 1104 1203 Size: 377 59 668 99 Fragments arranged by size: 668 377 99 59 XhoI C'TCGA_G Cuts at: 0 859 1203 Size: 859 344 XmaI C'CCGG_G Cuts at: 0 22 601 1203 Size: 22 579 602 Fragments arranged by size: 602 579 22 Enzymes that do cut: AarI AcuI AlfI AlwNI ApaI AscI AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PpuMI PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI SfcI SfoI SgrAI SmaI SmlI SrfI StyI TaqII XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PvuI SalI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XmnI ZraI