Restriction map for
pFN21AE1394
(Linear) (Six-base) MAPSORT of: KIEE1394.seq Check: 3056 from: 1 to: 1566
KIEE1394.orf
With 182 enzymes: SgfI *
January 28, 2010 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 672 1152 1566
Size: 672 480 414
Fragments arranged by size:
672 480 414
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1071 1256 1471 1566
Size: 1064 7 185 215 95
Fragments arranged by size:
1064 215 185 95 7
AflII C'TTAA_G
Cuts at: 0 516 1566
Size: 516 1050
AleI CACnn'nnGTG
Cuts at: 0 707 1566
Size: 707 859
AlwNI CAG_nnn'CTG
Cuts at: 0 673 1566
Size: 673 893
ApoI r'AATT_y
Cuts at: 0 46 152 1302 1566
Size: 46 106 1150 264
Fragments arranged by size:
1150 264 106 46
BamHI G'GATC_C
Cuts at: 0 197 717 1566
Size: 197 520 849
Fragments arranged by size:
849 520 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 599 1010 1566
Size: 599 411 556
Fragments arranged by size:
599 556 411
BfrBI ATG'CAT
Cuts at: 0 99 1566
Size: 99 1467
BglI GCCn_nnn'nGGC
Cuts at: 0 247 1566
Size: 247 1319
BglII A'GATC_T
Cuts at: 0 484 1566
Size: 484 1082
BmgBI CAC'GTC
Cuts at: 0 921 1566
Size: 921 645
BmrI ACTGGGnnnn_n'
Cuts at: 0 754 1566
Size: 754 812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 1255 1566
Size: 167 1088 311
Fragments arranged by size:
1088 311 167
BsaAI yAC'GTr
Cuts at: 0 684 1566
Size: 684 882
BsaBI GATnn'nnATC
Cuts at: 0 196 1566
Size: 196 1370
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 165 195 287 317 1566
Size: 165 30 92 30 1249
Fragments arranged by size:
1249 165 92 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 898 1252 1566
Size: 170 728 354 314
Fragments arranged by size:
728 354 314 170
BsiHKAI G_wGCw'C
Cuts at: 0 1143 1566
Size: 1143 423
Bsp1286I G_dGCh'C
Cuts at: 0 1143 1566
Size: 1143 423
BspHI T'CATG_A
Cuts at: 0 752 1566
Size: 752 814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 672 1152 1566
Size: 672 480 414
Fragments arranged by size:
672 480 414
BsrDI GCAATG_nn'
Cuts at: 0 81 227 446 731 1494 1566
Size: 81 146 219 285 763 72
Fragments arranged by size:
763 285 219 146 81 72
BsrFI r'CCGG_y
Cuts at: 0 1035 1566
Size: 1035 531
BsrGI T'GTAC_A
Cuts at: 0 1292 1566
Size: 1292 274
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 1566
Size: 744 822
BstYI r'GATC_y
Cuts at: 0 197 484 613 717 1465 1566
Size: 197 287 129 104 748 101
Fragments arranged by size:
748 287 197 129 104 101
BtgI C'CryG_G
Cuts at: 0 428 965 1566
Size: 428 537 601
Fragments arranged by size:
601 537 428
EaeI y'GGCC_r
Cuts at: 0 425 1566
Size: 425 1141
EarI CTCTTCn'nnn_
Cuts at: 0 981 1149 1409 1566
Size: 981 168 260 157
Fragments arranged by size:
981 260 168 157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 1064 1071 1255 1256 1471 1566
Size: 167 897 7 184 1 215 95
Fragments arranged by size:
897 215 184 167 95 7 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 317 1566
Size: 317 1249
EcoRI G'AATT_C
Cuts at: 0 46 1566
Size: 46 1520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 165 197 1566
Size: 98 32 35 32 1369
Fragments arranged by size:
1369 98 35 32 32
HincII GTy'rAC
Cuts at: 0 957 1566
Size: 957 609
MfeI C'AATT_G
Cuts at: 0 569 1566
Size: 569 997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1521 1566
Size: 1521 45
MscI TGG'CCA
Cuts at: 0 427 1566
Size: 427 1139
MslI CAynn'nnrTG
Cuts at: 0 217 553 707 857 1566
Size: 217 336 154 150 709
Fragments arranged by size:
709 336 217 154 150
MspA1I CmG'CkG
Cuts at: 0 1109 1566
Size: 1109 457
NcoI C'CATG_G
Cuts at: 0 428 965 1566
Size: 428 537 601
Fragments arranged by size:
601 537 428
NsiI A_TGCA'T
Cuts at: 0 101 1566
Size: 101 1465
NspI r_CATG'y
Cuts at: 0 103 1566
Size: 103 1463
PfoI T'CCnGG_A
Cuts at: 0 183 1566
Size: 183 1383
PsiI TTA'TAA
Cuts at: 0 481 1566
Size: 481 1085
PstI C_TGCA'G
Cuts at: 0 669 909 1473 1566
Size: 669 240 564 93
Fragments arranged by size:
669 564 240 93
SapI GCTCTTCn'nnn_
Cuts at: 0 1149 1566
Size: 1149 417
SfcI C'TryA_G
Cuts at: 0 665 905 1318 1398 1469 1566
Size: 665 240 413 80 71 97
Fragments arranged by size:
665 413 240 97 80 71
SmlI C'TyrA_G
Cuts at: 0 516 1566
Size: 516 1050
SspI AAT'ATT
Cuts at: 0 834 1566
Size: 834 732
StuI AGG'CCT
Cuts at: 0 12 39 1566
Size: 12 27 1527
Fragments arranged by size:
1527 27 12
StyI C'CwwG_G
Cuts at: 0 428 965 1566
Size: 428 537 601
Fragments arranged by size:
601 537 428
TatI w'GTAC_w
Cuts at: 0 741 1292 1418 1566
Size: 741 551 126 148
Fragments arranged by size:
741 551 148 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 668 700 1023 1055 1566
Size: 668 32 323 32 511
Fragments arranged by size:
668 511 323 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 155 1049 1566
Size: 155 894 517
Fragments arranged by size:
894 517 155
Enzymes that do cut:
AarI AcuI AflII AleI AlwNI ApoI BamHI BbsI
BfrBI BglI BglII BmgBI BmrI BpmI BsaAI BsaBI
BsaXI BseRI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstXI BstYI BtgI EaeI EarI Eco57MI EcoNI
EcoRI Hin4I HincII MfeI MmeI MscI MslI MspA1I
NcoI NsiI NspI PfoI PsiI PstI SapI SfcI
SmlI SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI
BclI BlpI Bme1580I BmtI BplI Bpu10I BpuEI BsaI
BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI