Restriction map for
pFN21AE1402
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BpuAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MfeI MflI MluI
MluNI MscI MunI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 679 GTAGAC GT^[AC][GT]AC
AccII 69 CGCG CG^CG
AlwI 1295 GGATC GGATC(4/5)
AvrII 1599 CCTAGG C^CTAGG
BfaI 1600 CTAG C^TAG
BlnI 1599 CCTAGG C^CTAGG
BlpI 949 GCTGAGC GC^T[ACGT]AGC
BpmI 709 CTGGAG CTGGAG(16/14)
Bpu1102I 949 GCTGAGC GC^T[ACGT]AGC
BsaBI 1023 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1033 GAGGAG GAGGAG(10/8)
BspMI 938 ACCTGC ACCTGC(4/8)
BsrBI 52 CCGCTC CCGCTC(-3/-3)
BsrDI 1125 CATTGC GCAATG(2/0)
BstUI 69 CGCG CG^CG
CelII 949 GCTGAGC GC^T[ACGT]AGC
DpnII 1296 GATC ^GATC
EarI 796 GAAGAG CTCTTC(1/4)
EcoNI 939 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 234 GAATTC G^AATTC
HgaI 714 GCGTC GACGC(5/10)
Ksp632I 796 GAAGAG CTCTTC(1/4)
MaeI 1600 CTAG C^TAG
MamI 1023 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1296 GATC ^GATC
MvnI 69 CGCG CG^CG
NdeII 1296 GATC ^GATC
NspI 1332 GCATGT [GA]CATG^[CT]
PpuMI 645 GGGTCCT [GA]G^G[AT]CC[CT]
Sau3AI 1296 GATC ^GATC
ScaI 1625 AGTACT AGT^ACT
SexAI 758 ACCAGGT A^CC[AT]GGT
StuI 662 AGGCCT AGG^CCT
TaqI 1031 TCGA T^CGA
TthHB8I 1031 TCGA T^CGA
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccIII 230 TCCGGA T^CCGGA
1620 TCCGGA
AcsI 234 GAATTC [GA]^AATT[CT]
1075 AAATTT
AcyI 81 GGCGCC G[GA]^CG[CT]C
801 GGCGCC
ApoI 234 GAATTC [GA]^AATT[CT]
1075 AAATTT
AvaI 824 CCCGAG C^[CT]CG[GA]G
1361 CTCGGG
BbeI 81 GGCGCC GGCGC^C
801 GGCGCC
BsaAI 914 TACGTG [CT]AC^GT[GA]
1039 TACGTG
BsaHI 81 GGCGCC G[GA]^CG[CT]C
801 GGCGCC
BsaI 1012 GAGACC GGTCTC(1/5)
1594 GGTCTC
BseAI 230 TCCGGA T^CCGGA
1620 TCCGGA
BsmFI 374 GTCCC GGGAC(10/14)
1721 GTCCC
BsoBI 824 CCCGAG C^[CT]CG[GA]G
1361 CTCGGG
BspEI 230 TCCGGA T^CCGGA
1620 TCCGGA
DraII 645 GGGTCCT [GA]G^G[ACGT]CC[CT]
1256 AGGCCCT
EcoO109I 645 GGGTCCT [GA]G^G[ACGT]CC[CT]
1256 AGGCCCT
Hin1I 81 GGCGCC G[GA]^CG[CT]C
801 GGCGCC
KasI 81 GGCGCC G^GCGCC
801 GGCGCC
MroI 230 TCCGGA T^CCGGA
1620 TCCGGA
MseI 737 TTAA T^TAA
1664 TTAA
NarI 81 GGCGCC GG^CGCC
801 GGCGCC
PstI 1066 CTGCAG CTGCA^G
1375 CTGCAG
SacI 1435 GAGCTC GAGCT^C
1792 GAGCTC
SfcI 1066 CTGCAG C^T[GA][CT]AG
1375 CTGCAG
SfoI 81 GGCGCC GGC^GCC
801 GGCGCC
SmlI 751 CTCAAG C^T[CT][GA]AG
1588 CTCAAG
TfiI 96 GAATC G^A[AT]TC
1605 GAATC
Tru9I 737 TTAA T^TAA
1664 TTAA
Tsp45I 167 GTGAC ^GT[GC]AC
935 GTGAC
XcmI 759 CCAGGTGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
861 CCAGGAGGTGTTTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbvI BmyI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NcoI NlaIII NlaIV
PflMI PleI PvuII RsaI Sau96I
ScrFI SfaNI StyI TseI Tsp509I
TspRI Van91I