(Circular) (Six-base) MAPSORT of: pFN21AE1402.seq Check: 5752 from: 1 to: 6502 pFN21AE1402 6502 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2685 3845 1937 Size: 748 1160 4594 Fragments arranged by size: 4594 1160 748 Acc65I G'GTAC_C Cuts at: 3823 3823 Size: 6502 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2969 3713 4565 5949 6217 1632 Size: 1337 744 852 1384 268 1917 Fragments arranged by size: 1917 1384 1337 852 744 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 5401 6378 1203 Size: 4198 977 1327 Fragments arranged by size: 4198 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 2096 2931 1166 Size: 930 835 4737 Fragments arranged by size: 4737 930 835 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6502 AlwNI CAG_nnn'CTG Cuts at: 2530 2770 3055 4245 5817 2530 Size: 240 285 1190 1572 3215 Fragments arranged by size: 3215 1572 1190 285 240 ApaLI G'TGCA_C Cuts at: 4559 5715 4559 Size: 1156 5346 ApoI r'AATT_y Cuts at: 1493 2239 3080 3811 4037 4189 6298 1493 Size: 746 841 731 226 152 2109 1697 Fragments arranged by size: 2109 1697 841 746 731 226 152 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 2829 3366 3827 1870 Size: 73 886 537 461 4545 Fragments arranged by size: 4545 886 537 461 73 AvrII C'CTAG_G Cuts at: 3604 3604 Size: 6502 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2744 2777 2744 Size: 33 6469 BamHI G'GATC_C Cuts at: 3832 4199 3832 Size: 367 6135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2579 2806 3162 3823 Size: 325 217 627 299 493 227 356 661 Cuts at: 3823 5278 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 661 627 493 356 325 299 227 217 BanII G_rGCy'C Cuts at: 1391 2895 3197 3444 3583 3801 3821 1391 Size: 1504 302 247 139 218 20 4072 Fragments arranged by size: 4072 1504 302 247 218 139 20 BbeI G_GCGC'C Cuts at: 2090 2810 2090 Size: 720 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4693 4727 1903 Size: 34 2756 34 3678 Fragments arranged by size: 3678 2756 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 1548 Size: 6502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5113 136 Size: 107 122 71 631 605 3441 1525 Fragments arranged by size: 3441 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6497 6497 Size: 6502 BlpI GC'TnA_GC Cuts at: 2955 3919 2955 Size: 964 5538 Bme1580I G_kGCm'C Cuts at: 1790 4563 5719 1790 Size: 2773 1156 2573 Fragments arranged by size: 2773 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2370 4233 5191 Size: 416 53 83 399 24 953 1863 958 Cuts at: 5191 442 Size: 1753 Fragments arranged by size: 1863 1753 958 953 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2351 2443 2351 Size: 92 6410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 5162 1239 Size: 1496 2427 2579 Fragments arranged by size: 2579 2427 1496 Bpu10I CC'TnA_GC Cuts at: 2549 2549 Size: 6502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2741 3578 4627 5492 5790 2741 Size: 837 1049 865 298 3453 Fragments arranged by size: 3453 1049 865 837 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3011 3605 5165 882 Size: 617 1512 594 1560 2219 Fragments arranged by size: 2219 1560 1512 617 594 BsaAI yAC'GTr Cuts at: 493 1204 2921 3046 4293 493 Size: 711 1717 125 1247 2702 Fragments arranged by size: 2702 1717 1247 711 125 BsaBI GATnn'nnATC Cuts at: 3032 6496 3032 Size: 3464 3038 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2087 2807 4309 4691 Size: 53 83 186 936 554 720 1502 382 Cuts at: 4691 5326 275 Size: 635 1451 Fragments arranged by size: 1502 1451 936 720 635 554 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2025 2235 3625 4935 5333 5607 5754 6165 2025 Size: 210 1390 1310 398 274 147 411 2362 Fragments arranged by size: 2362 1390 1310 411 398 274 210 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 3467 3497 1178 Size: 30 165 30 1305 30 729 30 4183 Fragments arranged by size: 4183 1305 729 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3053 1186 Size: 1867 4635 BseYI C'CCAG_C Cuts at: 2252 2415 2523 5705 6201 2252 Size: 163 108 3182 496 2553 Fragments arranged by size: 3182 2553 496 163 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2161 2191 3068 1163 Size: 573 425 30 877 4597 Fragments arranged by size: 4597 877 573 425 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3952 4713 5741 1540 Size: 275 186 1951 761 1028 2301 Fragments arranged by size: 2301 1951 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2928 2961 3444 3647 3801 4563 4648 5719 2928 Size: 33 483 203 154 762 85 1071 3711 Fragments arranged by size: 3711 1071 762 483 203 154 85 33 BsmI GAATG_Cn' Cuts at: 4013 4106 4013 Size: 93 6409 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2895 2928 2961 3197 3444 3583 3647 Size: 399 1105 33 33 236 247 139 64 Cuts at: 3647 3801 3821 4563 4648 5719 1391 Size: 154 20 742 85 1071 2174 Fragments arranged by size: 2174 1105 1071 742 399 247 236 154 139 85 64 33 33 20 BspEI T'CCGG_A Cuts at: 2235 3625 6165 2235 Size: 1390 2540 2572 Fragments arranged by size: 2572 2540 1390 BspHI T'CATG_A Cuts at: 4391 6121 4391 Size: 1730 4772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2952 3857 844 Size: 2108 905 3489 Fragments arranged by size: 3489 2108 905 BsrBI CCG'CTC Cuts at: 1394 1823 2059 4389 1394 Size: 429 236 2330 3507 Fragments arranged by size: 3507 2330 429 236 BsrDI GCAATG_nn' Cuts at: 65 1248 3129 4997 5171 65 Size: 1183 1881 1868 174 1396 Fragments arranged by size: 1881 1868 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5146 5333 1700 Size: 110 3336 187 2869 Fragments arranged by size: 3336 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6502 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 4562 5574 4562 Size: 1012 5490 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6502 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstXI CCAn_nnnn'nTGG Cuts at: 2488 2671 2702 3244 3293 2488 Size: 183 31 542 49 5697 Fragments arranged by size: 5697 542 183 49 31 BstYI r'GATC_y Cuts at: 1972 3832 4199 4584 4601 6053 6139 6151 6497 Size: 1860 367 385 17 1452 86 12 346 Cuts at: 6497 1972 Size: 1977 Fragments arranged by size: 1977 1860 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2487 2677 3135 513 Size: 552 1422 190 458 3880 Fragments arranged by size: 3880 1422 552 458 190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2988 487 Size: 683 56 1762 4001 Fragments arranged by size: 4001 1762 683 56 BtsI GCAGTG_nn' Cuts at: 2243 4018 4812 4832 2243 Size: 1775 794 20 3913 Fragments arranged by size: 3913 1775 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2692 3295 3684 Size: 54 1425 50 275 18 862 603 389 Cuts at: 3684 3949 4838 6467 8 Size: 265 889 1629 43 Fragments arranged by size: 1629 1425 889 862 603 389 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2796 4432 1353 Size: 1443 1636 3423 Fragments arranged by size: 3423 1636 1443 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3442 3799 3442 Size: 357 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2735 2969 3713 4565 5162 5949 6217 Size: 393 1103 234 744 852 597 787 268 Cuts at: 6217 1239 Size: 1524 Fragments arranged by size: 1524 1103 852 787 744 597 393 268 234 EcoNI CCTnn'n_nnAGG Cuts at: 2948 2948 Size: 6502 EcoO109I rG'GnC_Cy Cuts at: 2651 3262 2651 Size: 611 5891 EcoRI G'AATT_C Cuts at: 2239 3811 4189 2239 Size: 1572 378 4552 Fragments arranged by size: 4552 1572 378 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 2090 2293 2810 5649 1678 Size: 412 203 517 2839 2531 Fragments arranged by size: 2839 2531 517 412 203 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 3263 3295 3565 Size: 32 135 32 427 32 1232 32 270 Cuts at: 3565 3597 3773 3805 5143 5175 5217 5249 1373 Size: 32 176 32 1338 32 42 32 2626 Fragments arranged by size: 2626 1338 1232 427 270 176 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3846 4092 669 Size: 988 281 1908 246 3079 Fragments arranged by size: 3079 1908 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6502 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KasI G'GCGC_C Cuts at: 2086 2806 2086 Size: 720 5782 KpnI G_GTAC'C Cuts at: 3827 3827 Size: 6502 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2388 2454 3453 3483 5616 5800 1305 Size: 1083 66 999 30 2133 184 2007 Fragments arranged by size: 2133 2007 1083 999 184 66 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4680 Fragments arranged by size: 4680 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 2486 2700 2931 3134 3236 3242 Size: 648 930 390 214 231 203 102 6 Cuts at: 3242 4460 4819 4978 518 Size: 1218 359 159 2042 Fragments arranged by size: 2042 1218 930 648 390 359 231 214 203 159 102 6 MspA1I CmG'CkG Cuts at: 1606 2415 3055 3076 3918 4595 5743 5988 1606 Size: 809 640 21 842 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 809 677 640 245 21 NarI GG'CG_CC Cuts at: 2087 2807 2087 Size: 720 5782 NcoI C'CATG_G Cuts at: 513 1065 2487 2677 3135 513 Size: 552 1422 190 458 3880 Fragments arranged by size: 3880 1422 552 458 190 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 3341 3860 5405 1132 Size: 97 2112 519 1545 2229 Fragments arranged by size: 2229 2112 1545 519 97 PasI CC'CwG_GG Cuts at: 1348 2081 1348 Size: 733 5769 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 2770 2913 1108 Size: 1422 240 143 4697 Fragments arranged by size: 4697 1422 240 143 PfoI T'CCnGG_A Cuts at: 3435 3435 Size: 6502 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3083 3115 4571 4603 6084 6116 3083 Size: 32 1456 32 1481 32 3469 Fragments arranged by size: 3469 1481 1456 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2651 2651 Size: 6502 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PstI C_TGCA'G Cuts at: 830 3075 3384 3854 830 Size: 2245 309 470 3478 Fragments arranged by size: 3478 2245 470 309 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 2415 3055 3076 2415 Size: 640 21 5841 Fragments arranged by size: 5841 640 21 SacI G_AGCT'C Cuts at: 3444 3801 3444 Size: 357 6145 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 3632 4750 6462 3632 Size: 1118 1712 3672 Fragments arranged by size: 3672 1712 1118 SexAI A'CCwGG_T Cuts at: 2763 2763 Size: 6502 SfcI C'TryA_G Cuts at: 826 1045 3071 3380 3850 4985 5666 5857 826 Size: 219 2026 309 470 1135 681 191 1471 Fragments arranged by size: 2026 1471 1135 681 470 309 219 191 SfoI GGC'GCC Cuts at: 2088 2808 2088 Size: 720 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 3829 1872 Size: 1957 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 3593 4606 5507 5769 820 Size: 197 926 813 837 1013 901 262 1553 Fragments arranged by size: 1553 1013 926 901 837 813 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 2669 1517 Size: 1152 5350 StyI C'CwwG_G Cuts at: 513 1065 2487 2575 2664 2670 2677 3135 3604 Size: 552 1422 88 89 6 7 458 469 Cuts at: 3604 3941 513 Size: 337 3074 Fragments arranged by size: 3074 1422 552 469 458 337 89 88 7 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 2500 2633 4529 4546 4699 4884 Size: 1255 702 218 133 1896 17 153 185 Cuts at: 4884 5329 325 Size: 445 1498 Fragments arranged by size: 1896 1498 1255 702 445 218 185 153 133 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3204 3630 4748 Size: 274 80 33 51 1443 1227 426 1118 Cuts at: 4748 6460 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1227 1118 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 3838 3838 Size: 6502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2771 2873 2771 Size: 102 6400 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 3827 1870 Size: 1957 4545 XmnI GAAnn'nnTTC Cuts at: 4631 4631 Size: 6502 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI