(Linear) (Six-base) MAPSORT of: KIEE1402.seq Check: 2496 from: 1 to: 1797 KIEE1402.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AccI GT'mk_AC Cuts at: 0 680 1797 Size: 680 1117 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 964 1708 1797 Size: 964 744 89 Fragments arranged by size: 964 744 89 AleI CACnn'nnGTG Cuts at: 0 91 926 1797 Size: 91 835 871 Fragments arranged by size: 871 835 91 AlwNI CAG_nnn'CTG Cuts at: 0 525 765 1050 1797 Size: 525 240 285 747 Fragments arranged by size: 747 525 285 240 ApoI r'AATT_y Cuts at: 0 234 1075 1797 Size: 234 841 722 Fragments arranged by size: 841 722 234 AvaI C'yCGr_G Cuts at: 0 824 1361 1797 Size: 824 537 436 Fragments arranged by size: 824 537 436 AvrII C'CTAG_G Cuts at: 0 1599 1797 Size: 1599 198 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 739 772 1797 Size: 739 33 1025 Fragments arranged by size: 1025 739 33 BanI G'GyrC_C Cuts at: 0 81 574 801 1157 1797 Size: 81 493 227 356 640 Fragments arranged by size: 640 493 356 227 81 BanII G_rGCy'C Cuts at: 0 890 1192 1439 1578 1796 1797 Size: 890 302 247 139 218 1 Fragments arranged by size: 890 302 247 218 139 1 BbeI G_GCGC'C Cuts at: 0 85 805 1797 Size: 85 720 992 Fragments arranged by size: 992 720 85 BlpI GC'TnA_GC Cuts at: 0 950 1797 Size: 950 847 BmrI ACTGGGnnnn_n' Cuts at: 0 365 1797 Size: 365 1432 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 346 438 1797 Size: 346 92 1359 Fragments arranged by size: 1359 346 92 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 1797 Size: 730 1067 Bpu10I CC'TnA_GC Cuts at: 0 544 1797 Size: 544 1253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 736 1573 1797 Size: 736 837 224 Fragments arranged by size: 837 736 224 BsaI GGTCTCn'nnnn_ Cuts at: 0 1006 1600 1797 Size: 1006 594 197 Fragments arranged by size: 1006 594 197 BsaAI yAC'GTr Cuts at: 0 916 1041 1797 Size: 916 125 756 Fragments arranged by size: 916 756 125 BsaBI GATnn'nnATC Cuts at: 0 1027 1797 Size: 1027 770 BsaHI Gr'CG_yC Cuts at: 0 82 802 1797 Size: 82 720 995 Fragments arranged by size: 995 720 82 BsaWI w'CCGG_w Cuts at: 0 20 230 1620 1797 Size: 20 210 1390 177 Fragments arranged by size: 1390 210 177 20 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 703 733 1462 1492 1797 Size: 703 30 729 30 305 Fragments arranged by size: 729 703 305 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1048 1797 Size: 1048 749 BseYI C'CCAG_C Cuts at: 0 247 410 518 1797 Size: 247 163 108 1279 Fragments arranged by size: 1279 247 163 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 156 186 1063 1797 Size: 156 30 877 734 Fragments arranged by size: 877 734 156 30 BsiHKAI G_wGCw'C Cuts at: 0 923 956 1439 1642 1796 1797 Size: 923 33 483 203 154 1 Fragments arranged by size: 923 483 203 154 33 1 Bsp1286I G_dGCh'C Cuts at: 0 890 923 956 1192 1439 1578 1642 1796 Size: 890 33 33 236 247 139 64 154 Cuts at: 1796 1797 Size: 1 Fragments arranged by size: 890 247 236 154 139 64 33 33 1 BspEI T'CCGG_A Cuts at: 0 230 1620 1797 Size: 230 1390 177 Fragments arranged by size: 1390 230 177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 947 1797 Size: 947 850 BsrBI CCG'CTC Cuts at: 0 54 1797 Size: 54 1743 BsrDI GCAATG_nn' Cuts at: 0 1124 1797 Size: 1124 673 BstXI CCAn_nnnn'nTGG Cuts at: 0 483 666 697 1239 1288 1797 Size: 483 183 31 542 49 509 Fragments arranged by size: 542 509 483 183 49 31 BtgI C'CryG_G Cuts at: 0 482 672 1130 1797 Size: 482 190 458 667 Fragments arranged by size: 667 482 458 190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 983 1797 Size: 983 814 BtsI GCAGTG_nn' Cuts at: 0 238 1797 Size: 238 1559 EaeI y'GGCC_r Cuts at: 0 687 1290 1679 1797 Size: 687 603 389 118 Fragments arranged by size: 687 603 389 118 EarI CTCTTCn'nnn_ Cuts at: 0 791 1797 Size: 791 1006 EcoICRI GAG'CTC Cuts at: 0 1437 1794 1797 Size: 1437 357 3 Fragments arranged by size: 1437 357 3 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 964 1708 1797 Size: 730 234 744 89 Fragments arranged by size: 744 730 234 89 EcoNI CCTnn'n_nnAGG Cuts at: 0 943 1797 Size: 943 854 EcoO109I rG'GnC_Cy Cuts at: 0 646 1257 1797 Size: 646 611 540 Fragments arranged by size: 646 611 540 EcoRI G'AATT_C Cuts at: 0 234 1797 Size: 234 1563 HaeII r_GCGC'y Cuts at: 0 85 288 805 1797 Size: 85 203 517 992 Fragments arranged by size: 992 517 203 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 26 1258 1290 1560 1592 1768 1797 Size: 26 1232 32 270 32 176 29 Fragments arranged by size: 1232 270 176 32 32 29 26 KasI G'GCGC_C Cuts at: 0 81 801 1797 Size: 81 720 996 Fragments arranged by size: 996 720 81 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 383 449 1448 1478 1797 Size: 383 66 999 30 319 Fragments arranged by size: 999 383 319 66 30 MslI CAynn'nnrTG Cuts at: 0 91 481 695 926 1129 1231 1237 1797 Size: 91 390 214 231 203 102 6 560 Fragments arranged by size: 560 390 231 214 203 102 91 6 MspA1I CmG'CkG Cuts at: 0 410 1050 1071 1797 Size: 410 640 21 726 Fragments arranged by size: 726 640 410 21 NarI GG'CG_CC Cuts at: 0 82 802 1797 Size: 82 720 995 Fragments arranged by size: 995 720 82 NcoI C'CATG_G Cuts at: 0 482 672 1130 1797 Size: 482 190 458 667 Fragments arranged by size: 667 482 458 190 NspI r_CATG'y Cuts at: 0 1336 1797 Size: 1336 461 PasI CC'CwG_GG Cuts at: 0 76 1797 Size: 76 1721 PflMI CCAn_nnn'nTGG Cuts at: 0 525 765 908 1797 Size: 525 240 143 889 Fragments arranged by size: 889 525 240 143 PfoI T'CCnGG_A Cuts at: 0 1430 1797 Size: 1430 367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1078 1110 1797 Size: 1078 32 687 Fragments arranged by size: 1078 687 32 PpuMI rG'GwC_Cy Cuts at: 0 646 1797 Size: 646 1151 PstI C_TGCA'G Cuts at: 0 1070 1379 1797 Size: 1070 309 418 Fragments arranged by size: 1070 418 309 PvuII CAG'CTG Cuts at: 0 410 1050 1071 1797 Size: 410 640 21 726 Fragments arranged by size: 726 640 410 21 SacI G_AGCT'C Cuts at: 0 1439 1796 1797 Size: 1439 357 1 Fragments arranged by size: 1439 357 1 ScaI AGT'ACT Cuts at: 0 1627 1797 Size: 1627 170 SexAI A'CCwGG_T Cuts at: 0 758 1797 Size: 758 1039 SfcI C'TryA_G Cuts at: 0 1066 1375 1797 Size: 1066 309 422 Fragments arranged by size: 1066 422 309 SfoI GGC'GCC Cuts at: 0 83 803 1797 Size: 83 720 994 Fragments arranged by size: 994 720 83 SmlI C'TyrA_G Cuts at: 0 751 1588 1797 Size: 751 837 209 Fragments arranged by size: 837 751 209 StuI AGG'CCT Cuts at: 0 664 1797 Size: 664 1133 StyI C'CwwG_G Cuts at: 0 482 570 659 665 672 1130 1599 1797 Size: 482 88 89 6 7 458 469 198 Fragments arranged by size: 482 469 458 198 89 88 7 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 277 495 628 1797 Size: 277 218 133 1169 Fragments arranged by size: 1169 277 218 133 TatI w'GTAC_w Cuts at: 0 1199 1625 1797 Size: 1199 426 172 Fragments arranged by size: 1199 426 172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 766 868 1797 Size: 766 102 929 Fragments arranged by size: 929 766 102 Enzymes that do cut: AccI AcuI AleI AlwNI ApoI AvaI AvrII BaeI BanI BanII BbeI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BstXI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI MslI MspA1I NarI NcoI NspI PasI PflMI PfoI PpiI PpuMI PstI PvuII SacI ScaI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BsiEI BsiWI BsmI BsmBI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI