(Circular) (Six-base) MAPSORT of: pFN21AB5907.seq Check: 4593 from: 1 to: 6217 pFN21AB5907 6217 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2150 3560 1937 Size: 213 1410 4594 Fragments arranged by size: 4594 1410 213 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2140 2669 2685 4280 5664 5932 1632 Size: 508 529 16 1595 1384 268 1917 Fragments arranged by size: 1917 1595 1384 529 508 268 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2044 1184 Size: 860 5357 AlwNI CAG_nnn'CTG Cuts at: 2065 2647 3960 5532 2065 Size: 582 1313 1572 2750 Fragments arranged by size: 2750 1572 1313 582 ApaLI G'TGCA_C Cuts at: 2019 4274 5430 2019 Size: 2255 1156 2806 Fragments arranged by size: 2806 2255 1156 ApoI r'AATT_y Cuts at: 1493 2620 2682 3526 3752 3904 6013 1493 Size: 1127 62 844 226 152 2109 1697 Fragments arranged by size: 2109 1697 1127 844 226 152 62 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2568 2580 2995 3542 1870 Size: 73 625 12 415 547 4545 Fragments arranged by size: 4545 625 547 415 73 12 AvrII C'CTAG_G Cuts at: 2960 2960 Size: 6217 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2369 3200 3305 3538 4993 Size: 325 217 627 582 831 105 233 1455 Cuts at: 4993 618 Size: 1842 Fragments arranged by size: 1842 1455 831 627 582 325 233 217 105 BanII G_rGCy'C Cuts at: 1391 2699 3536 1391 Size: 1308 837 4072 Fragments arranged by size: 4072 1308 837 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BbvCI CC'TCA_GC Cuts at: 3334 3334 Size: 6217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2703 4828 136 Size: 107 122 71 631 605 1031 2125 1525 Fragments arranged by size: 2125 1525 1031 631 605 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2109 3634 2109 Size: 1525 4692 Bme1580I G_kGCm'C Cuts at: 1790 2023 2372 4278 5434 1790 Size: 233 349 1906 1156 2573 Fragments arranged by size: 2573 1906 1156 349 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2783 2783 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2309 2480 2584 3108 3470 4877 1239 Size: 1040 30 171 104 524 362 1407 2579 Fragments arranged by size: 2579 1407 1040 524 362 171 104 30 Bpu10I CC'TnA_GC Cuts at: 2114 3334 2114 Size: 1220 4997 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2333 2814 2846 4880 882 Size: 617 834 481 32 2034 2219 Fragments arranged by size: 2219 2034 834 617 481 32 BsaAI yAC'GTr Cuts at: 493 1204 2616 2859 4008 493 Size: 711 1412 243 1149 2702 Fragments arranged by size: 2702 1412 1149 711 243 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2732 4650 5048 5322 5469 5880 2732 Size: 1918 398 274 147 411 3069 Fragments arranged by size: 3069 1918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2733 2763 2843 2873 1178 Size: 30 165 30 1330 30 80 30 4522 Fragments arranged by size: 4522 1330 165 80 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2429 1186 Size: 1102 141 4974 Fragments arranged by size: 4974 1102 141 BseYI C'CCAG_C Cuts at: 2365 5420 5916 2365 Size: 3055 496 2666 Fragments arranged by size: 3055 2666 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5644 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2023 4278 4363 5434 2023 Size: 2255 85 1071 2806 Fragments arranged by size: 2806 2255 1071 85 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2372 2699 3536 4278 4363 5434 Size: 399 233 349 327 837 742 85 1071 Cuts at: 5434 1391 Size: 2174 Fragments arranged by size: 2174 1071 837 742 399 349 327 233 85 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2386 4104 1394 Size: 429 563 1718 3507 Fragments arranged by size: 3507 1718 563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4861 5048 1700 Size: 110 3051 187 2869 Fragments arranged by size: 3051 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3312 96 Size: 3216 3001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2512 2416 Size: 96 6121 BstYI r'GATC_y Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212 Size: 1575 367 385 17 1452 86 12 346 Cuts at: 6212 1972 Size: 1977 Fragments arranged by size: 1977 1575 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2151 2151 Size: 6217 Bsu36I CC'TnA_GG Cuts at: 2375 2375 Size: 6217 BtgI C'CryG_G Cuts at: 513 1065 3100 513 Size: 552 2035 3630 Fragments arranged by size: 3630 2035 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2574 3733 4527 4547 2574 Size: 1159 794 20 4244 Fragments arranged by size: 4244 1159 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2163 2502 Size: 54 1425 50 275 18 307 26 339 Cuts at: 2502 3664 4553 6182 8 Size: 1162 889 1629 43 Fragments arranged by size: 1629 1425 1162 889 339 307 275 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 4147 1353 Size: 1107 1687 3423 Fragments arranged by size: 3423 1687 1107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2879 4804 5178 5324 387 Size: 1064 138 1290 1925 374 146 1280 Fragments arranged by size: 1925 1290 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2279 2309 2480 2584 2669 2685 Size: 393 508 139 30 171 104 85 16 Cuts at: 2685 3108 3470 4280 4877 5664 5932 1239 Size: 423 362 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 597 508 423 393 362 268 171 139 104 85 30 16 EcoO109I rG'GnC_Cy Cuts at: 2331 2331 Size: 6217 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3478 3478 Size: 6217 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 3021 5364 1678 Size: 1343 2343 2531 Fragments arranged by size: 2531 2343 1343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2684 2716 4858 4890 4932 Size: 32 135 32 1112 32 2142 32 42 Cuts at: 4932 4964 1373 Size: 32 2626 Fragments arranged by size: 2626 2142 1112 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3352 3561 3807 669 Size: 988 281 1414 209 246 3079 Fragments arranged by size: 3079 1414 988 281 246 209 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 2306 3816 2306 Size: 1510 4707 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 2493 5331 5515 1305 Size: 1092 96 2838 184 2007 Fragments arranged by size: 2838 2007 1092 184 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 2504 10 Size: 54 1425 343 333 339 3723 Fragments arranged by size: 3723 1425 343 339 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 4175 4534 4693 518 Size: 648 3009 359 159 2042 Fragments arranged by size: 3009 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2062 2184 2395 3633 4310 5458 5703 Size: 448 8 122 211 1238 677 1148 245 Cuts at: 5703 1606 Size: 2120 Fragments arranged by size: 2120 1238 1148 677 448 245 211 122 8 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5665 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PfoI T'CCnGG_A Cuts at: 2640 2640 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PmlI CAC'GTG Cuts at: 2859 2859 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PstI C_TGCA'G Cuts at: 830 2033 2358 3569 830 Size: 1203 325 1211 3478 Fragments arranged by size: 3478 1211 1203 325 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2054 2062 2184 2395 2054 Size: 8 122 211 5876 Fragments arranged by size: 5876 211 122 8 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2029 2354 2428 3565 4700 5381 5572 Size: 219 984 325 74 1137 1135 681 191 Cuts at: 5572 826 Size: 1471 Fragments arranged by size: 1471 1137 1135 984 681 325 219 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 5222 5484 820 Size: 197 926 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 2746 152 Size: 2594 3623 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 3165 1517 Size: 1648 4569 StyI C'CwwG_G Cuts at: 513 1065 2313 2960 3656 513 Size: 552 1248 647 696 3074 Fragments arranged by size: 3074 1248 696 647 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 2861 4244 4261 4414 4599 5044 Size: 1255 678 603 1383 17 153 185 445 Cuts at: 5044 325 Size: 1498 Fragments arranged by size: 1498 1383 1255 678 603 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2991 3312 3420 Size: 274 80 33 51 1443 1014 321 108 Cuts at: 3420 4463 6175 96 Size: 1043 1712 138 Fragments arranged by size: 1712 1443 1043 1014 321 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2843 2875 6177 6209 2843 Size: 32 3302 32 2851 Fragments arranged by size: 3302 2851 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI