Restriction map for
pFN21AB5907
(Linear) (Six-base) MAPSORT of: KIBB5907.seq Check: 6321 from: 1 to: 1512
KIBB5907.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 0 145 1512
Size: 145 1367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 664 680 1512
Size: 135 529 16 832
Fragments arranged by size:
832 529 135 16
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 39 1512
Size: 39 1473
AlwNI CAG_nnn'CTG
Cuts at: 0 60 642 1512
Size: 60 582 870
Fragments arranged by size:
870 582 60
ApaLI G'TGCA_C
Cuts at: 0 14 1512
Size: 14 1498
ApoI r'AATT_y
Cuts at: 0 615 677 1512
Size: 615 62 835
Fragments arranged by size:
835 615 62
AvaI C'yCGr_G
Cuts at: 0 563 575 990 1512
Size: 563 12 415 522
Fragments arranged by size:
563 522 415 12
AvrII C'CTAG_G
Cuts at: 0 955 1512
Size: 955 557
BanI G'GyrC_C
Cuts at: 0 364 1195 1300 1512
Size: 364 831 105 212
Fragments arranged by size:
831 364 212 105
BanII G_rGCy'C
Cuts at: 0 694 1512
Size: 694 818
BbvCI CC'TCA_GC
Cuts at: 0 1329 1512
Size: 1329 183
BglI GCCn_nnn'nGGC
Cuts at: 0 698 1512
Size: 698 814
BlpI GC'TnA_GC
Cuts at: 0 104 1512
Size: 104 1408
Bme1580I G_kGCm'C
Cuts at: 0 18 367 1512
Size: 18 349 1145
Fragments arranged by size:
1145 349 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 778 1512
Size: 778 734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 304 475 579 1103 1465 1512
Size: 274 30 171 104 524 362 47
Fragments arranged by size:
524 362 274 171 104 47 30
Bpu10I CC'TnA_GC
Cuts at: 0 109 1329 1512
Size: 109 1220 183
Fragments arranged by size:
1220 183 109
BsaI GGTCTCn'nnnn_
Cuts at: 0 328 809 841 1512
Size: 328 481 32 671
Fragments arranged by size:
671 481 328 32
BsaAI yAC'GTr
Cuts at: 0 611 854 1512
Size: 611 243 658
Fragments arranged by size:
658 611 243
BsaWI w'CCGG_w
Cuts at: 0 727 1512
Size: 727 785
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 728 758 838 868 1512
Size: 728 30 80 30 644
Fragments arranged by size:
728 644 80 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 424 1512
Size: 283 141 1088
Fragments arranged by size:
1088 283 141
BseYI C'CCAG_C
Cuts at: 0 360 1512
Size: 360 1152
BsiHKAI G_wGCw'C
Cuts at: 0 18 1512
Size: 18 1494
Bsp1286I G_dGCh'C
Cuts at: 0 18 367 694 1512
Size: 18 349 327 818
Fragments arranged by size:
818 349 327 18
BsrBI CCG'CTC
Cuts at: 0 381 1512
Size: 381 1131
BsrGI T'GTAC_A
Cuts at: 0 1307 1512
Size: 1307 205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 507 1512
Size: 411 96 1005
Fragments arranged by size:
1005 411 96
BstZ17I GTA'TAC
Cuts at: 0 146 1512
Size: 146 1366
Bsu36I CC'TnA_GG
Cuts at: 0 370 1512
Size: 370 1142
BtgI C'CryG_G
Cuts at: 0 1095 1512
Size: 1095 417
BtsI GCAGTG_nn'
Cuts at: 0 569 1512
Size: 569 943
EaeI y'GGCC_r
Cuts at: 0 132 158 497 1512
Size: 132 26 339 1015
Fragments arranged by size:
1015 339 132 26
EarI CTCTTCn'nnn_
Cuts at: 0 455 1512
Size: 455 1057
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 874 1512
Size: 874 638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 274 304 475 579 664 680 1103
Size: 135 139 30 171 104 85 16 423
Cuts at: 1103 1465 1512
Size: 362 47
Fragments arranged by size:
423 362 171 139 135 104 85 47
30 16
EcoO109I rG'GnC_Cy
Cuts at: 0 326 1512
Size: 326 1186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1473 1512
Size: 1473 39
HaeII r_GCGC'y
Cuts at: 0 1016 1512
Size: 1016 496
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 1512
Size: 679 32 801
Fragments arranged by size:
801 679 32
HincII GTy'rAC
Cuts at: 0 1347 1512
Size: 1347 165
MfeI C'AATT_G
Cuts at: 0 301 1512
Size: 301 1211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 488 1512
Size: 392 96 1024
Fragments arranged by size:
1024 392 96
MscI TGG'CCA
Cuts at: 0 160 499 1512
Size: 160 339 1013
Fragments arranged by size:
1013 339 160
MspA1I CmG'CkG
Cuts at: 0 49 57 179 390 1512
Size: 49 8 122 211 1122
Fragments arranged by size:
1122 211 122 49 8
PfoI T'CCnGG_A
Cuts at: 0 635 1512
Size: 635 877
PmlI CAC'GTG
Cuts at: 0 854 1512
Size: 854 658
PstI C_TGCA'G
Cuts at: 0 28 353 1512
Size: 28 325 1159
Fragments arranged by size:
1159 325 28
PvuII CAG'CTG
Cuts at: 0 49 57 179 390 1512
Size: 49 8 122 211 1122
Fragments arranged by size:
1122 211 122 49 8
SfcI C'TryA_G
Cuts at: 0 24 349 423 1512
Size: 24 325 74 1089
Fragments arranged by size:
1089 325 74 24
SpeI A'CTAG_T
Cuts at: 0 741 1512
Size: 741 771
StuI AGG'CCT
Cuts at: 0 1160 1512
Size: 1160 352
StyI C'CwwG_G
Cuts at: 0 308 955 1512
Size: 308 647 557
Fragments arranged by size:
647 557 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 856 1512
Size: 253 603 656
Fragments arranged by size:
656 603 253
TatI w'GTAC_w
Cuts at: 0 986 1307 1415 1512
Size: 986 321 108 97
Fragments arranged by size:
986 321 108 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 838 870 1512
Size: 838 32 642
Fragments arranged by size:
838 642 32
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AvaI AvrII
BanI BanII BbvCI BglI BlpI Bme1580I BplI BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BsrBI BsrGI BstXI BstZ17I Bsu36I BtgI BtsI
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
HincII MfeI MmeI MscI MspA1I PfoI PmlI PstI
PvuII SfcI SpeI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI BpuEI BsaBI BsaHI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI