Restriction map for
pFN21AE4471
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HgaI Hin1I
HindIII KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AseI 58 ATTAAT AT^TAAT
AsnI 58 ATTAAT AT^TAAT
Asp700I 586 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1393 GTGCTC G[AT]GC[AT]^C
AvaI 109 CCCGGG C^[CT]CG[GA]G
AvaII 1199 GGACC G^G[AT]CC
BalI 1220 TGGCCA TGG^CCA
BanI 1119 GGTGCC G^G[CT][GA]CC
BglII 434 AGATCT A^GATCT
BmyI 1393 GTGCTC G[AGT]GC[ACT]^C
BpmI 1132 CTGGAG CTGGAG(16/14)
BsaI 1333 GAGACC GGTCTC(1/5)
BseRI 1272 CTCCTC GAGGAG(10/8)
BsiHKAI 1393 GTGCTC G[AT]GC[AT]^C
BsoBI 109 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1393 GTGCTC G[AGT]GC[ACT]^C
BsrDI 1290 CATTGC GCAATG(2/0)
BsrFI 239 ACCGGC [GA]^CCGG[CT]
CfoI 1256 GCGC GCG^C
Cfr10I 239 ACCGGC [GA]^CCGG[CT]
DraII 1127 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 1218 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1127 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 577 ATGCAT ATGCA^T
HaeII 1255 GGCGCT [GA]GCGC^[CT]
HhaI 1256 GCGC GCG^C
HinP1I 1256 GCGC G^CGC
HpaI 552 GTTAAC GTT^AAC
MluNI 1220 TGGCCA TGG^CCA
MscI 1220 TGGCCA TGG^CCA
MspA1I 647 CAGCTG C[AC]G^C[GT]G
NsiI 577 ATGCAT ATGCA^T
PflMI 1010 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 58 ATTAAT AT^TAAT
PvuII 647 CAGCTG CAG^CTG
SmaI 109 CCCGGG CCC^GGG
SspI 61 AATATT AAT^ATT
Van91I 1010 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1403 CCATATTCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 109 CCCGGG C^CCGGG
XmaI 109 CCCGGG C^CCGGG
XmnI 586 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 266 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
317 CAGACACTG
BbsI 279 GAAGAC GAAGAC(2/6)
218 GTCTTC
BfaI 428 CTAG C^TAG
770 CTAG
BpuAI 279 GAAGAC GAAGAC(2/6)
218 GTCTTC
BsaAI 292 TACGTG [CT]AC^GT[GA]
1312 TACGTG
BsaWI 444 ACCGGA [AT]^CCGG[AT]
764 TCCGGT
BsgI 1052 CTGCAC GTGCAG(16/14)
1315 GTGCAG
BsmAI 1333 GAGAC GTCTC(1/5)
144 GTCTC
BspHI 791 TCATGA T^CATGA
860 TCATGA
BstF5I 600 GGATG GGATG(2/0)
705 GGATG
BstYI 401 AGATCC [GA]^GATC[CT]
434 AGATCT
EaeI 1007 CGGCCA [CT]^GGCC[GA]
1220 TGGCCA
FauI 1376 CCCGC CCCGC(4/6)
137 GCGGG
FokI 600 GGATG GGATG(9/13)
705 GGATG
MaeI 428 CTAG C^TAG
770 CTAG
MaeII 293 ACGT A^CGT
1313 ACGT
MflI 401 AGATCC [GA]^GATC[CT]
434 AGATCT
MseI 59 TTAA T^TAA
553 TTAA
NcoI 596 CCATGG C^CATGG
1010 CCATGG
NdeI 998 CATATG CA^TATG
1430 CATATG
NspI 153 GCATGC [GA]CATG^[CT]
606 GCATGC
RcaI 791 TCATGA T^CATGA
860 TCATGA
SfaNI 262 GATGC GCATC(5/9)
1379 GCATC
SmlI 811 CTCAAG C^T[CT][GA]AG
960 CTTGAG
SphI 153 GCATGC GCATG^C
606 GCATGC
Tru9I 59 TTAA T^TAA
553 TTAA
XhoII 401 AGATCC [GA]^GATC[CT]
434 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BcnI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI StyI
TfiI TseI Tsp45I Tsp509I TspRI