(Circular) (Six-base) MAPSORT of: pFN21AE4471.seq Check: 4118 from: 1 to: 6181 pFN21AE4471 6181 bp With 182 enzymes: SgfI * March 28, 2012 14:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3524 1937 Size: 1587 4594 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2298 2645 2676 4244 5628 5896 1632 Size: 666 347 31 1568 1384 268 1917 Fragments arranged by size: 1917 1568 1384 666 347 268 31 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 2945 3317 1166 Size: 1779 372 4030 Fragments arranged by size: 4030 1779 372 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3140 1184 Size: 1956 4225 AlwNI CAG_nnn'CTG Cuts at: 2276 2327 3924 5496 2276 Size: 51 1597 1572 2961 Fragments arranged by size: 2961 1597 1572 51 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 2387 2983 3068 3239 3375 3490 3716 3868 Size: 894 596 85 171 136 115 226 152 Cuts at: 3868 5977 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 894 596 226 171 152 136 115 85 AseI AT'TA_AT Cuts at: 160 1032 2064 4735 160 Size: 872 1032 2671 1606 Fragments arranged by size: 2671 1606 1032 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2114 3506 1870 Size: 73 171 1392 4545 Fragments arranged by size: 4545 1392 171 73 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3124 3502 4957 618 Size: 325 217 627 1337 378 1455 1842 Fragments arranged by size: 1842 1455 1337 627 378 325 217 BanII G_rGCy'C Cuts at: 1391 3500 1391 Size: 2109 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 2291 928 Size: 971 317 75 4818 Fragments arranged by size: 4818 971 317 75 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BfrBI ATG'CAT Cuts at: 2584 2584 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4792 136 Size: 107 122 71 631 605 3120 1525 Fragments arranged by size: 3120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2439 6176 2439 Size: 3737 2444 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 4242 5398 1790 Size: 2452 1156 2573 Fragments arranged by size: 2573 2452 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3264 3912 4870 Size: 416 53 83 399 24 1847 648 958 Cuts at: 4870 442 Size: 1753 Fragments arranged by size: 1847 1753 958 648 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3158 4841 1239 Size: 1919 1683 2579 Fragments arranged by size: 2579 1919 1683 Bpu10I CC'TnA_GC Cuts at: 2154 2154 Size: 6181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2801 2986 4306 5171 5469 2801 Size: 185 1320 865 298 3513 Fragments arranged by size: 3513 1320 865 298 185 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3332 4844 882 Size: 617 1833 1512 2219 Fragments arranged by size: 2219 1833 1512 617 BsaAI yAC'GTr Cuts at: 493 1204 2299 3319 3972 493 Size: 711 1095 1020 653 2702 Fragments arranged by size: 2702 1095 1020 711 653 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4370 5005 275 Size: 53 83 186 936 2455 382 635 1451 Fragments arranged by size: 2455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2449 2769 4614 5012 5286 5433 5844 2449 Size: 320 1845 398 274 147 411 2786 Fragments arranged by size: 2786 1845 411 398 320 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2256 2286 2775 2805 1178 Size: 30 165 30 853 30 489 30 4554 Fragments arranged by size: 4554 853 489 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3268 1186 Size: 2082 4099 BseYI C'CCAG_C Cuts at: 3166 5384 5880 3166 Size: 2218 496 3467 Fragments arranged by size: 3467 2218 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3042 3341 1163 Size: 573 1306 299 4003 Fragments arranged by size: 4003 1306 573 299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3631 4392 5420 1540 Size: 275 186 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3402 4242 4327 5398 3402 Size: 840 85 1071 4185 Fragments arranged by size: 4185 1071 840 85 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3402 3500 4242 4327 5398 1391 Size: 399 1612 98 742 85 1071 2174 Fragments arranged by size: 2174 1612 1071 742 399 98 85 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 2796 2865 4070 5800 2796 Size: 69 1205 1730 3177 Fragments arranged by size: 3177 1730 1205 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3536 844 Size: 2692 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3294 4676 4850 65 Size: 1183 2046 1382 174 1396 Fragments arranged by size: 2046 1396 1382 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2244 4825 5012 1700 Size: 110 434 2581 187 2869 Fragments arranged by size: 2869 2581 434 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6181 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6181 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2587 2753 3223 2215 Size: 372 166 470 5173 Fragments arranged by size: 5173 470 372 166 BstYI r'GATC_y Cuts at: 1972 2406 2439 3511 3878 4263 4280 5732 5818 Size: 434 33 1072 367 385 17 1452 86 Cuts at: 5818 5830 6176 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1072 434 385 367 346 86 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2601 2694 3015 513 Size: 552 1536 93 321 3679 Fragments arranged by size: 3679 1536 552 321 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5442 Fragments arranged by size: 5442 683 56 BtsI GCAGTG_nn' Cuts at: 2277 3697 4491 4511 2277 Size: 1420 794 20 3947 Fragments arranged by size: 3947 1420 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3012 3225 3628 Size: 54 1425 50 275 18 1182 213 403 Cuts at: 3628 4517 6146 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1182 889 403 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3628 1537 Size: 275 1816 4090 Fragments arranged by size: 4090 1816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4111 1353 Size: 2758 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2645 2676 3158 4244 4841 5628 Size: 393 666 347 31 482 1086 597 787 Cuts at: 5628 5896 1239 Size: 268 1524 Fragments arranged by size: 1524 1086 787 666 597 482 393 347 268 31 EcoNI CCTnn'n_nnAGG Cuts at: 3227 3227 Size: 6181 EcoO109I rG'GnC_Cy Cuts at: 3133 3133 Size: 6181 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 3264 5328 1678 Size: 1586 2064 2531 Fragments arranged by size: 2531 2064 1586 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2775 2807 3196 3228 4822 Size: 32 135 32 1203 32 389 32 1594 Cuts at: 4822 4854 4896 4928 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1594 1203 389 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2559 3082 3370 3442 3525 3771 Size: 988 281 621 523 288 72 83 246 Cuts at: 3771 669 Size: 3079 Fragments arranged by size: 3079 988 621 523 288 281 246 83 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 2559 3771 2559 Size: 1212 4969 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5295 5479 1305 Size: 3990 184 2007 Fragments arranged by size: 3990 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3227 10 Size: 54 1425 343 1395 2964 Fragments arranged by size: 2964 1425 1395 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2330 2606 2945 3317 4139 4498 Size: 648 1131 33 276 339 372 822 359 Cuts at: 4498 4657 518 Size: 159 2042 Fragments arranged by size: 2042 1131 822 648 372 359 339 276 159 33 MspA1I CmG'CkG Cuts at: 1606 2654 3597 4274 5422 5667 1606 Size: 1048 943 677 1148 245 2120 Fragments arranged by size: 2120 1148 1048 943 677 245 NcoI C'CATG_G Cuts at: 513 1065 2601 3015 513 Size: 552 1536 414 3679 Fragments arranged by size: 3679 1536 552 414 NdeI CA'TA_TG Cuts at: 387 3004 3436 387 Size: 2617 432 3132 Fragments arranged by size: 3132 2617 432 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NsiI A_TGCA'T Cuts at: 2586 2586 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 2162 2615 3539 5084 1132 Size: 97 933 453 924 1545 2229 Fragments arranged by size: 2229 1545 933 924 453 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6181 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 3021 1108 Size: 1913 4268 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2898 2930 4250 4282 5763 5795 2898 Size: 32 1320 32 1481 32 3284 Fragments arranged by size: 3284 1481 1320 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PstI C_TGCA'G Cuts at: 830 3533 830 Size: 2703 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2654 2654 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SfcI C'TryA_G Cuts at: 826 1045 3529 4664 5345 5536 826 Size: 219 2484 1135 681 191 1471 Fragments arranged by size: 2484 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 2116 3508 1872 Size: 244 1392 4545 Fragments arranged by size: 4545 1392 244 SmlI C'TyrA_G Cuts at: 820 1017 1943 2816 2965 4285 5186 5448 820 Size: 197 926 873 149 1320 901 262 1553 Fragments arranged by size: 1553 1320 926 901 873 262 197 149 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 2162 2615 3539 1132 Size: 1030 453 924 3774 Fragments arranged by size: 3774 1030 924 453 SspI AAT'ATT Cuts at: 5 52 2068 4105 5 Size: 47 2016 2037 2081 Fragments arranged by size: 2081 2037 2016 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6181 StyI C'CwwG_G Cuts at: 513 1065 2044 2601 3015 3228 3255 3306 3620 Size: 552 979 557 414 213 27 51 314 Cuts at: 3620 513 Size: 3074 Fragments arranged by size: 3074 979 557 552 414 314 213 51 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3319 4208 4225 4378 4563 5008 325 Size: 1255 1739 889 17 153 185 445 1498 Fragments arranged by size: 1739 1498 1255 889 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 2777 4427 Size: 274 80 33 51 1443 360 440 1650 Cuts at: 4427 6139 96 Size: 1712 138 Fragments arranged by size: 1712 1650 1443 440 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2256 2288 6141 6173 2256 Size: 32 3853 32 2264 Fragments arranged by size: 3853 2264 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3415 3415 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 2114 3506 1870 Size: 244 1392 4545 Fragments arranged by size: 4545 1392 244 XmnI GAAnn'nnTTC Cuts at: 2595 4310 2595 Size: 1715 4466 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI