Restriction map for pFN21AE4471
 (Linear) (Six-base) MAPSORT of: KIEE4471.seq  Check: 5250  from: 1  to: 1476



KIEE4471.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     640     671    1476 
   Size:        293     347      31     805

  Fragments arranged by size: 

                 805     347     293      31
 
AleI CACnn'nnGTG

Cuts at:      0     940    1312    1476 
   Size:        940     372     164

  Fragments arranged by size: 

                 940     372     164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1135    1476 
   Size:       1135     341
 
AlwNI CAG_nnn'CTG

Cuts at:      0     271     322    1476 
   Size:        271      51    1154

  Fragments arranged by size: 

                1154     271      51
 
ApoI r'AATT_y

Cuts at:      0     382     978    1063    1234    1370    1476 
   Size:        382     596      85     171     136     106

  Fragments arranged by size: 

                 596     382     171     136     106      85
 
AseI AT'TA_AT

Cuts at:      0      59    1476 
   Size:         59    1417
 
AvaI C'yCGr_G

Cuts at:      0     109    1476 
   Size:        109    1367
 
BanI G'GyrC_C

Cuts at:      0    1119    1476 
   Size:       1119     357
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     286    1476 
   Size:        211      75    1190

  Fragments arranged by size: 

                1190     211      75
 
BfrBI ATG'CAT

Cuts at:      0     579    1476 
   Size:        579     897
 
BglII A'GATC_T

Cuts at:      0     434    1476 
   Size:        434    1042
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1259    1476 
   Size:       1259     217
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1153    1476 
   Size:       1153     323
 
Bpu10I CC'TnA_GC

Cuts at:      0     149    1476 
   Size:        149    1327
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796     981    1476 
   Size:        796     185     495

  Fragments arranged by size: 

                 796     495     185
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1327    1476 
   Size:       1327     149
 
BsaAI yAC'GTr

Cuts at:      0     294    1314    1476 
   Size:        294    1020     162

  Fragments arranged by size: 

                1020     294     162
 
BsaWI w'CCGG_w

Cuts at:      0     444     764    1476 
   Size:        444     320     712

  Fragments arranged by size: 

                 712     444     320
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     251     281     770     800    1476 
   Size:        251      30     489      30     676

  Fragments arranged by size: 

                 676     489     251      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1263    1476 
   Size:       1263     213
 
BseYI C'CCAG_C

Cuts at:      0    1161    1476 
   Size:       1161     315
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1336    1476 
   Size:       1037     299     140

  Fragments arranged by size: 

                1037     299     140
 
BsiHKAI G_wGCw'C

Cuts at:      0    1397    1476 
   Size:       1397      79
 
Bsp1286I G_dGCh'C

Cuts at:      0    1397    1476 
   Size:       1397      79
 
BspHI T'CATG_A

Cuts at:      0     791     860    1476 
   Size:        791      69     616

  Fragments arranged by size: 

                 791     616      69
 
BsrDI GCAATG_nn'

Cuts at:      0    1289    1476 
   Size:       1289     187
 
BsrFI r'CCGG_y

Cuts at:      0     239    1476 
   Size:        239    1237
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     582     748    1218    1476 
   Size:        210     372     166     470     258

  Fragments arranged by size: 

                 470     372     258     210     166
 
BstYI r'GATC_y

Cuts at:      0     401     434    1476 
   Size:        401      33    1042

  Fragments arranged by size: 

                1042     401      33
 
BtgI C'CryG_G

Cuts at:      0     596     689    1010    1476 
   Size:        596      93     321     466

  Fragments arranged by size: 

                 596     466     321      93
 
BtsI GCAGTG_nn'

Cuts at:      0     272    1476 
   Size:        272    1204
 
EaeI y'GGCC_r

Cuts at:      0    1007    1220    1476 
   Size:       1007     213     256

  Fragments arranged by size: 

                1007     256     213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     640     671    1153    1476 
   Size:        293     347      31     482     323

  Fragments arranged by size: 

                 482     347     323     293      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1222    1476 
   Size:       1222     254
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1128    1476 
   Size:       1128     348
 
HaeII r_GCGC'y

Cuts at:      0    1259    1476 
   Size:       1259     217
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     770     802    1191    1223    1476 
   Size:        770      32     389      32     253

  Fragments arranged by size: 

                 770     389     253      32      32
 
HincII GTy'rAC

Cuts at:      0     554    1077    1365    1437    1476 
   Size:        554     523     288      72      39

  Fragments arranged by size: 

                 554     523     288      72      39
 
HpaI GTT'AAC

Cuts at:      0     554    1476 
   Size:        554     922
 
MscI TGG'CCA

Cuts at:      0    1222    1476 
   Size:       1222     254
 
MslI CAynn'nnrTG

Cuts at:      0     292     325     601     940    1312    1476 
   Size:        292      33     276     339     372     164

  Fragments arranged by size: 

                 372     339     292     276     164      33
 
MspA1I CmG'CkG

Cuts at:      0     649    1476 
   Size:        649     827
 
NcoI C'CATG_G

Cuts at:      0     596    1010    1476 
   Size:        596     414     466

  Fragments arranged by size: 

                 596     466     414
 
NdeI CA'TA_TG

Cuts at:      0     999    1431    1476 
   Size:        999     432      45

  Fragments arranged by size: 

                 999     432      45
 
NsiI A_TGCA'T

Cuts at:      0     581    1476 
   Size:        581     895
 
NspI r_CATG'y

Cuts at:      0     157     610    1476 
   Size:        157     453     866

  Fragments arranged by size: 

                 866     453     157
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1016    1476 
   Size:       1016     460
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     893     925    1476 
   Size:        893      32     551

  Fragments arranged by size: 

                 893     551      32
 
PvuII CAG'CTG

Cuts at:      0     649    1476 
   Size:        649     827
 
SmaI CCC'GGG

Cuts at:      0     111    1476 
   Size:        111    1365
 
SmlI C'TyrA_G

Cuts at:      0     811     960    1476 
   Size:        811     149     516

  Fragments arranged by size: 

                 811     516     149
 
SphI G_CATG'C

Cuts at:      0     157     610    1476 
   Size:        157     453     866

  Fragments arranged by size: 

                 866     453     157
 
SspI AAT'ATT

Cuts at:      0      63    1476 
   Size:         63    1413
 
StyI C'CwwG_G

Cuts at:      0      39     596    1010    1223    1250    1301    1476 
   Size:         39     557     414     213      27      51     175

  Fragments arranged by size: 

                 557     414     213     175      51      39      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1314    1476 
   Size:       1314     162
 
TatI w'GTAC_w

Cuts at:      0     332     772    1476 
   Size:        332     440     704

  Fragments arranged by size: 

                 704     440     332
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     251     283    1476 
   Size:        251      32    1193

  Fragments arranged by size: 

                1193     251      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1410    1476 
   Size:       1410      66
 
XmaI C'CCGG_G

Cuts at:      0     109    1476 
   Size:        109    1367
 
XmnI GAAnn'nnTTC

Cuts at:      0     590    1476 
   Size:        590     886
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApoI     AseI     AvaI     BanI
     BbsI    BfrBI    BglII     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrDI    BsrFI    BstXI    BstYI     BtgI     BtsI     EaeI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     HpaI     MscI
     MslI   MspA1I     NcoI     NdeI     NsiI     NspI    PflMI     PpiI
    PvuII     SmaI     SmlI     SphI     SspI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaBI    BsaHI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
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