(Linear) (Six-base) MAPSORT of: KIEE4471.seq Check: 5250 from: 1 to: 1476 KIEE4471.orf With 182 enzymes: SgfI * March 22, 2012 18:08 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 293 640 671 1476 Size: 293 347 31 805 Fragments arranged by size: 805 347 293 31 AleI CACnn'nnGTG Cuts at: 0 940 1312 1476 Size: 940 372 164 Fragments arranged by size: 940 372 164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1135 1476 Size: 1135 341 AlwNI CAG_nnn'CTG Cuts at: 0 271 322 1476 Size: 271 51 1154 Fragments arranged by size: 1154 271 51 ApoI r'AATT_y Cuts at: 0 382 978 1063 1234 1370 1476 Size: 382 596 85 171 136 106 Fragments arranged by size: 596 382 171 136 106 85 AseI AT'TA_AT Cuts at: 0 59 1476 Size: 59 1417 AvaI C'yCGr_G Cuts at: 0 109 1476 Size: 109 1367 BanI G'GyrC_C Cuts at: 0 1119 1476 Size: 1119 357 BbsI GAAGACnn'nnnn_ Cuts at: 0 211 286 1476 Size: 211 75 1190 Fragments arranged by size: 1190 211 75 BfrBI ATG'CAT Cuts at: 0 579 1476 Size: 579 897 BglII A'GATC_T Cuts at: 0 434 1476 Size: 434 1042 BmrI ACTGGGnnnn_n' Cuts at: 0 1259 1476 Size: 1259 217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1153 1476 Size: 1153 323 Bpu10I CC'TnA_GC Cuts at: 0 149 1476 Size: 149 1327 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 796 981 1476 Size: 796 185 495 Fragments arranged by size: 796 495 185 BsaI GGTCTCn'nnnn_ Cuts at: 0 1327 1476 Size: 1327 149 BsaAI yAC'GTr Cuts at: 0 294 1314 1476 Size: 294 1020 162 Fragments arranged by size: 1020 294 162 BsaWI w'CCGG_w Cuts at: 0 444 764 1476 Size: 444 320 712 Fragments arranged by size: 712 444 320 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 251 281 770 800 1476 Size: 251 30 489 30 676 Fragments arranged by size: 676 489 251 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1263 1476 Size: 1263 213 BseYI C'CCAG_C Cuts at: 0 1161 1476 Size: 1161 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1037 1336 1476 Size: 1037 299 140 Fragments arranged by size: 1037 299 140 BsiHKAI G_wGCw'C Cuts at: 0 1397 1476 Size: 1397 79 Bsp1286I G_dGCh'C Cuts at: 0 1397 1476 Size: 1397 79 BspHI T'CATG_A Cuts at: 0 791 860 1476 Size: 791 69 616 Fragments arranged by size: 791 616 69 BsrDI GCAATG_nn' Cuts at: 0 1289 1476 Size: 1289 187 BsrFI r'CCGG_y Cuts at: 0 239 1476 Size: 239 1237 BstXI CCAn_nnnn'nTGG Cuts at: 0 210 582 748 1218 1476 Size: 210 372 166 470 258 Fragments arranged by size: 470 372 258 210 166 BstYI r'GATC_y Cuts at: 0 401 434 1476 Size: 401 33 1042 Fragments arranged by size: 1042 401 33 BtgI C'CryG_G Cuts at: 0 596 689 1010 1476 Size: 596 93 321 466 Fragments arranged by size: 596 466 321 93 BtsI GCAGTG_nn' Cuts at: 0 272 1476 Size: 272 1204 EaeI y'GGCC_r Cuts at: 0 1007 1220 1476 Size: 1007 213 256 Fragments arranged by size: 1007 256 213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 293 640 671 1153 1476 Size: 293 347 31 482 323 Fragments arranged by size: 482 347 323 293 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 1222 1476 Size: 1222 254 EcoO109I rG'GnC_Cy Cuts at: 0 1128 1476 Size: 1128 348 HaeII r_GCGC'y Cuts at: 0 1259 1476 Size: 1259 217 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 770 802 1191 1223 1476 Size: 770 32 389 32 253 Fragments arranged by size: 770 389 253 32 32 HincII GTy'rAC Cuts at: 0 554 1077 1365 1437 1476 Size: 554 523 288 72 39 Fragments arranged by size: 554 523 288 72 39 HpaI GTT'AAC Cuts at: 0 554 1476 Size: 554 922 MscI TGG'CCA Cuts at: 0 1222 1476 Size: 1222 254 MslI CAynn'nnrTG Cuts at: 0 292 325 601 940 1312 1476 Size: 292 33 276 339 372 164 Fragments arranged by size: 372 339 292 276 164 33 MspA1I CmG'CkG Cuts at: 0 649 1476 Size: 649 827 NcoI C'CATG_G Cuts at: 0 596 1010 1476 Size: 596 414 466 Fragments arranged by size: 596 466 414 NdeI CA'TA_TG Cuts at: 0 999 1431 1476 Size: 999 432 45 Fragments arranged by size: 999 432 45 NsiI A_TGCA'T Cuts at: 0 581 1476 Size: 581 895 NspI r_CATG'y Cuts at: 0 157 610 1476 Size: 157 453 866 Fragments arranged by size: 866 453 157 PflMI CCAn_nnn'nTGG Cuts at: 0 1016 1476 Size: 1016 460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 893 925 1476 Size: 893 32 551 Fragments arranged by size: 893 551 32 PvuII CAG'CTG Cuts at: 0 649 1476 Size: 649 827 SmaI CCC'GGG Cuts at: 0 111 1476 Size: 111 1365 SmlI C'TyrA_G Cuts at: 0 811 960 1476 Size: 811 149 516 Fragments arranged by size: 811 516 149 SphI G_CATG'C Cuts at: 0 157 610 1476 Size: 157 453 866 Fragments arranged by size: 866 453 157 SspI AAT'ATT Cuts at: 0 63 1476 Size: 63 1413 StyI C'CwwG_G Cuts at: 0 39 596 1010 1223 1250 1301 1476 Size: 39 557 414 213 27 51 175 Fragments arranged by size: 557 414 213 175 51 39 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1314 1476 Size: 1314 162 TatI w'GTAC_w Cuts at: 0 332 772 1476 Size: 332 440 704 Fragments arranged by size: 704 440 332 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 251 283 1476 Size: 251 32 1193 Fragments arranged by size: 1193 251 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1410 1476 Size: 1410 66 XmaI C'CCGG_G Cuts at: 0 109 1476 Size: 109 1367 XmnI GAAnn'nnTTC Cuts at: 0 590 1476 Size: 590 886 Enzymes that do cut: AcuI AleI AlfI AlwNI ApoI AseI AvaI BanI BbsI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstXI BstYI BtgI BtsI EaeI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI MscI MslI MspA1I NcoI NdeI NsiI NspI PflMI PpiI PvuII SmaI SmlI SphI SspI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmtI BplI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XhoI ZraI