Restriction map for
pFN21AE1105
(Circular) (Six-base) MAPSORT of: pFN21AE1105.seq Check: 952 from: 1 to: 4987
pFN21AE1105 4987 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2797 278
Size: 53 83 186 936 1261 2468
Fragments arranged by size:
2468 1261 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2330 1937
Size: 393 4594
Acc65I G'GTAC_C
Cuts at: 2308 2308
Size: 4987
AclI AA'CG_TT
Cuts at: 1563 3114 3487 1563
Size: 1551 373 3063
Fragments arranged by size:
3063 1551 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3050 4434 4702 1632
Size: 1418 1384 268 1917
Fragments arranged by size:
1917 1418 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4790
AflIII A'CryG_T
Cuts at: 1203 3886 4863 1203
Size: 2683 977 1327
Fragments arranged by size:
2683 1327 977
AgeI A'CCGG_T
Cuts at: 3818 3818
Size: 4987
AhdI GACnn_n'nnGTC
Cuts at: 3716 3716
Size: 4987
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2299 1184
Size: 1115 3872
AlwNI CAG_nnn'CTG
Cuts at: 2730 4302 2730
Size: 1572 3415
ApaLI G'TGCA_C
Cuts at: 3044 4200 3044
Size: 1156 3831
ApoI r'AATT_y
Cuts at: 1493 2296 2522 2674 4783 1493
Size: 803 226 152 2109 1697
Fragments arranged by size:
2109 1697 803 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3541 160
Size: 872 2509 1606
Fragments arranged by size:
2509 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4987
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 1870
Size: 73 369 4545
Fragments arranged by size:
4545 369 73
BamHI G'GATC_C
Cuts at: 2317 2684 2317
Size: 367 4620
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2308 3763 618
Size: 325 217 627 521 1455 1842
Fragments arranged by size:
1842 1455 627 521 325 217
BanII G_rGCy'C
Cuts at: 1391 2306 1391
Size: 915 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2272 928
Size: 971 373 3643
Fragments arranged by size:
3643 971 373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3178 3212 1903
Size: 34 1241 34 3678
Fragments arranged by size:
3678 1241 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2879 4089 2879
Size: 1210 3777
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4987
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 3598 136
Size: 107 122 71 631 605 379 1547 1525
Fragments arranged by size:
1547 1525 631 605 379 122 107 71
BglII A'GATC_T
Cuts at: 4982 4982
Size: 4987
BlpI GC'TnA_GC
Cuts at: 2061 2404 2061
Size: 343 4644
Bme1580I G_kGCm'C
Cuts at: 1790 3048 4204 1790
Size: 1258 1156 2573
Fragments arranged by size:
2573 1258 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4987
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2718 3676 442
Size: 416 53 83 399 24 1301 958 1753
Fragments arranged by size:
1753 1301 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 3647 1239
Size: 815 1593 2579
Fragments arranged by size:
2579 1593 815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 3112 3977 4275 2167
Size: 945 865 298 2879
Fragments arranged by size:
2879 945 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3650 882
Size: 617 2151 2219
Fragments arranged by size:
2219 2151 617
BsaAI yAC'GTr
Cuts at: 493 1204 2778 493
Size: 711 1574 2702
Fragments arranged by size:
2702 1574 711
BsaBI GATnn'nnATC
Cuts at: 4981 4981
Size: 4987
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2794 3176 3811 275
Size: 53 83 186 936 1261 382 635 1451
Fragments arranged by size:
1451 1261 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3420 3818 4092 4239 4650 3420
Size: 398 274 147 411 3757
Fragments arranged by size:
3757 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4762
Fragments arranged by size:
4762 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4987
BseYI C'CCAG_C
Cuts at: 4190 4686 4190
Size: 496 4491
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2437 3198 4226 1540
Size: 275 186 436 761 1028 2301
Fragments arranged by size:
2301 1028 761 436 275 186
BsiHKAI G_wGCw'C
Cuts at: 3048 3133 4204 3048
Size: 85 1071 3831
Fragments arranged by size:
3831 1071 85
BsmI GAATG_Cn'
Cuts at: 2276 2498 2591 2276
Size: 222 93 4672
Fragments arranged by size:
4672 222 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2306 3048 3133 4204 1391
Size: 399 516 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 399 85
BspEI T'CCGG_A
Cuts at: 4650 4650
Size: 4987
BspHI T'CATG_A
Cuts at: 2876 4606 2876
Size: 1730 3257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2258 2342 844
Size: 1414 84 3489
Fragments arranged by size:
3489 1414 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2874 1394
Size: 429 1051 3507
Fragments arranged by size:
3507 1051 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2223 3482 3656 65
Size: 1183 975 1259 174 1396
Fragments arranged by size:
1396 1259 1183 975 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3631 3818 1700
Size: 110 1821 187 2869
Fragments arranged by size:
2869 1821 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4987
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4987
BssSI C'ACGA_G
Cuts at: 3047 4059 3047
Size: 1012 3975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4987
BstBI TT'CG_AA
Cuts at: 3791 3791
Size: 4987
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4987
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2038
Size: 4987
BstYI r'GATC_y
Cuts at: 1972 2317 2684 3069 3086 4538 4624 4636 4982
Size: 345 367 385 17 1452 86 12 346
Cuts at: 4982 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 345 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4435
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4248
Fragments arranged by size:
4248 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3297 3317 2503
Size: 794 20 4173
Fragments arranged by size:
4173 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4987
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4952
DraI TTT'AAA
Cuts at: 2291 2291
Size: 4987
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3994 809
Size: 507 2678 1802
Fragments arranged by size:
2678 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2434 3323 4952
Size: 54 1425 50 275 18 604 889 1629
Cuts at: 4952 8
Size: 43
Fragments arranged by size:
1629 1425 889 604 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2434 1537
Size: 275 622 4090
Fragments arranged by size:
4090 622 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2917 1353
Size: 1564 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3574 3948 4094 387
Size: 1064 138 1985 374 146 1280
Fragments arranged by size:
1985 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 3050 3647 4434 4702 1239
Size: 393 422 996 597 787 268 1524
Fragments arranged by size:
1524 996 787 597 422 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2010 2010
Size: 4987
EcoRI G'AATT_C
Cuts at: 2296 2674 2296
Size: 378 4609
FspI TGC'GCA
Cuts at: 3493 3493
Size: 4987
HaeII r_GCGC'y
Cuts at: 1678 4134 1678
Size: 2456 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3628 3660 3702 3734 1373
Size: 32 135 32 2056 32 42 32 2626
Fragments arranged by size:
2626 2056 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2331 2577 669
Size: 988 281 393 246 3079
Fragments arranged by size:
3079 988 393 281 246
HindIII A'AGCT_T
Cuts at: 748 2158 748
Size: 1410 3577
HpaI GTT'AAC
Cuts at: 2577 2577
Size: 4987
KpnI G_GTAC'C
Cuts at: 2312 2312
Size: 4987
MfeI C'AATT_G
Cuts at: 2586 2586
Size: 4987
MluI A'CGCG_T
Cuts at: 4863 4863
Size: 4987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4101 4285 1305
Size: 2796 184 2007
Fragments arranged by size:
2796 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3165
Fragments arranged by size:
3165 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 3304 3463 518
Size: 648 1779 359 159 2042
Fragments arranged by size:
2042 1779 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2403 3080 4228 4473 1606
Size: 511 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 511 286 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4435
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4987
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4987
NotI GC'GGCC_GC
Cuts at: 2434 2434
Size: 4987
NspI r_CATG'y
Cuts at: 1132 1229 2345 3890 1132
Size: 97 1116 1545 2229
Fragments arranged by size:
2229 1545 1116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4987
PciI A'CATG_T
Cuts at: 3886 3886
Size: 4987
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4987
PmeI GTTT'AAAC
Cuts at: 2291 2291
Size: 4987
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 4569 4601 3056
Size: 32 1481 32 3442
Fragments arranged by size:
3442 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2010 2010
Size: 4987
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4987
PsiI TTA'TAA
Cuts at: 2557 2557
Size: 4987
PstI C_TGCA'G
Cuts at: 830 2052 2098 2339 830
Size: 1222 46 241 3478
Fragments arranged by size:
3478 1222 241 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4987
SalI G'TCGA_C
Cuts at: 1936 2329 1936
Size: 393 4594
SbfI CC_TGCA'GG
Cuts at: 2052 2339 2052
Size: 287 4700
ScaI AGT'ACT
Cuts at: 3235 4947 3235
Size: 1712 3275
SfcI C'TryA_G
Cuts at: 826 1045 2048 2094 2335 3470 4151 4342 826
Size: 219 1003 46 241 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 681 241 219 191 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 2051 2051
Size: 4987
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4987
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4987
SmaI CCC'GGG
Cuts at: 1872 2314 1872
Size: 442 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2182 3091 3992 4254 820
Size: 197 926 239 909 901 262 1553
Fragments arranged by size:
1553 926 909 901 262 239 197
SnaBI TAC'GTA
Cuts at: 493 2778 493
Size: 2285 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4987
SphI G_CATG'C
Cuts at: 1132 2345 1132
Size: 1213 3774
SspI AAT'ATT
Cuts at: 5 52 2911 5
Size: 47 2859 2081
Fragments arranged by size:
2859 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4987
StyI C'CwwG_G
Cuts at: 513 1065 2426 513
Size: 552 1361 3074
Fragments arranged by size:
3074 1361 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3014 3031 3184 3369 3814 325
Size: 1255 1434 17 153 185 445 1498
Fragments arranged by size:
1498 1434 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3233 4945 96
Size: 274 80 33 51 1443 1256 1712 138
Fragments arranged by size:
1712 1443 1256 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4947 4979 4947
Size: 32 4955
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4987
XbaI T'CTAG_A
Cuts at: 2323 2323
Size: 4987
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 4987
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4987
XmaI C'CCGG_G
Cuts at: 1870 2312 1870
Size: 442 4545
XmnI GAAnn'nnTTC
Cuts at: 3116 3116
Size: 4987
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2795 276
Size: 53 83 186 936 1261 2468
Fragments arranged by size:
2468 1261 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI