(Linear) (Six-base) MAPSORT of: KIEE4531.seq Check: 7381 from: 1 to: 1782 KIEE4531.orf With 182 enzymes: SgfI * March 22, 2012 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 206 1637 1782 Size: 206 1431 145 Fragments arranged by size: 1431 206 145 Acc65I G'GTAC_C Cuts at: 0 1496 1782 Size: 1496 286 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 12 504 1782 Size: 12 492 1278 Fragments arranged by size: 1278 492 12 AleI CACnn'nnGTG Cuts at: 0 463 1782 Size: 463 1319 AlwNI CAG_nnn'CTG Cuts at: 0 444 972 1782 Size: 444 528 810 Fragments arranged by size: 810 528 444 ApaI G_GGCC'C Cuts at: 0 182 452 1400 1782 Size: 182 270 948 382 Fragments arranged by size: 948 382 270 182 AvaI C'yCGr_G Cuts at: 0 234 263 288 413 1581 1782 Size: 234 29 25 125 1168 201 Fragments arranged by size: 1168 234 201 125 29 25 BanI G'GyrC_C Cuts at: 0 165 342 420 1496 1782 Size: 165 177 78 1076 286 Fragments arranged by size: 1076 286 177 165 78 BanII G_rGCy'C Cuts at: 0 182 452 908 1400 1455 1588 1782 Size: 182 270 456 492 55 133 194 Fragments arranged by size: 492 456 270 194 182 133 55 BbeI G_GCGC'C Cuts at: 0 169 346 424 1782 Size: 169 177 78 1358 Fragments arranged by size: 1358 177 169 78 BbsI GAAGACnn'nnnn_ Cuts at: 0 1152 1178 1782 Size: 1152 26 604 Fragments arranged by size: 1152 604 26 BglI GCCn_nnn'nGGC Cuts at: 0 245 386 1306 1395 1782 Size: 245 141 920 89 387 Fragments arranged by size: 920 387 245 141 89 Bme1580I G_kGCm'C Cuts at: 0 182 452 1400 1782 Size: 182 270 948 382 Fragments arranged by size: 948 382 270 182 BmrI ACTGGGnnnn_n' Cuts at: 0 1104 1782 Size: 1104 678 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 626 692 1782 Size: 626 66 1090 Fragments arranged by size: 1090 626 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 213 637 1037 1492 1558 1741 1782 Size: 213 424 400 455 66 183 41 Fragments arranged by size: 455 424 400 213 183 66 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1363 1782 Size: 1363 419 BsaI GGTCTCn'nnnn_ Cuts at: 0 515 770 1782 Size: 515 255 1012 Fragments arranged by size: 1012 515 255 BsaAI yAC'GTr Cuts at: 0 465 1242 1782 Size: 465 777 540 Fragments arranged by size: 777 540 465 BsaHI Gr'CG_yC Cuts at: 0 166 343 421 1782 Size: 166 177 78 1361 Fragments arranged by size: 1361 177 166 78 BsaWI w'CCGG_w Cuts at: 0 714 908 1782 Size: 714 194 874 Fragments arranged by size: 874 714 194 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 83 113 1570 1600 1782 Size: 83 30 1457 30 182 Fragments arranged by size: 1457 182 83 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1009 1040 1782 Size: 1009 31 742 Fragments arranged by size: 1009 742 31 BseYI C'CCAG_C Cuts at: 0 307 444 1782 Size: 307 137 1338 Fragments arranged by size: 1338 307 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1222 1782 Size: 1222 560 BsiEI CG_ry'CG Cuts at: 0 53 260 1782 Size: 53 207 1522 Fragments arranged by size: 1522 207 53 BsiHKAI G_wGCw'C Cuts at: 0 908 1573 1782 Size: 908 665 209 Fragments arranged by size: 908 665 209 BsmI GAATG_Cn' Cuts at: 0 856 968 1782 Size: 856 112 814 Fragments arranged by size: 856 814 112 Bsp1286I G_dGCh'C Cuts at: 0 182 452 908 1400 1455 1573 1588 1782 Size: 182 270 456 492 55 118 15 194 Fragments arranged by size: 492 456 270 194 182 118 55 15 BspEI T'CCGG_A Cuts at: 0 714 1782 Size: 714 1068 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 206 713 1637 1782 Size: 206 507 924 145 Fragments arranged by size: 924 507 206 145 BsrBI CCG'CTC Cuts at: 0 83 270 949 1782 Size: 83 187 679 833 Fragments arranged by size: 833 679 187 83 BsrFI r'CCGG_y Cuts at: 0 380 1305 1782 Size: 380 925 477 Fragments arranged by size: 925 477 380 BssHII G'CGCG_C Cuts at: 0 109 111 278 302 1782 Size: 109 2 167 24 1480 Fragments arranged by size: 1480 167 109 24 2 BssSI C'ACGA_G Cuts at: 0 1636 1782 Size: 1636 146 BstAPI GCAn_nnn'nTGC Cuts at: 0 1632 1782 Size: 1632 150 BstXI CCAn_nnnn'nTGG Cuts at: 0 636 1301 1470 1782 Size: 636 665 169 312 Fragments arranged by size: 665 636 312 169 BstYI r'GATC_y Cuts at: 0 1286 1782 Size: 1286 496 BtgI C'CryG_G Cuts at: 0 243 689 982 1250 1421 1782 Size: 243 446 293 268 171 361 Fragments arranged by size: 446 361 293 268 243 171 BtsI GCAGTG_nn' Cuts at: 0 336 1326 1782 Size: 336 990 456 Fragments arranged by size: 990 456 336 DraIII CAC_nnn'GTG Cuts at: 0 210 1782 Size: 210 1572 DrdI GACnn_nn'nnGTC Cuts at: 0 1558 1782 Size: 1558 224 EaeI y'GGCC_r Cuts at: 0 257 314 356 692 1303 1614 1782 Size: 257 57 42 336 611 311 168 Fragments arranged by size: 611 336 311 257 168 57 42 EagI C'GGCC_G Cuts at: 0 257 1782 Size: 257 1525 EarI CTCTTCn'nnn_ Cuts at: 0 595 986 1561 1782 Size: 595 391 575 221 Fragments arranged by size: 595 575 391 221 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 104 1782 Size: 104 1678 EcoICRI GAG'CTC Cuts at: 0 906 1782 Size: 906 876 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 12 213 504 637 1037 1492 1558 1741 Size: 12 201 291 133 400 455 66 183 Cuts at: 1741 1782 Size: 41 Fragments arranged by size: 455 400 291 201 183 133 66 41 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1052 1782 Size: 1052 730 EcoO109I rG'GnC_Cy Cuts at: 0 407 448 816 1202 1396 1782 Size: 407 41 368 386 194 386 Fragments arranged by size: 407 386 386 368 194 41 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 609 1782 Size: 609 1173 FseI GG_CCGG'CC Cuts at: 0 384 1782 Size: 384 1398 FspI TGC'GCA Cuts at: 0 160 1782 Size: 160 1622 HaeII r_GCGC'y Cuts at: 0 157 169 346 368 424 1782 Size: 157 12 177 22 56 1358 Fragments arranged by size: 1358 177 157 56 22 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 933 965 1035 1067 1782 Size: 933 32 70 32 715 Fragments arranged by size: 933 715 70 32 32 HincII GTy'rAC Cuts at: 0 1275 1782 Size: 1275 507 KasI G'GCGC_C Cuts at: 0 165 342 420 1782 Size: 165 177 78 1362 Fragments arranged by size: 1362 177 165 78 KpnI G_GTAC'C Cuts at: 0 1500 1782 Size: 1500 282 MscI TGG'CCA Cuts at: 0 1616 1782 Size: 1616 166 MslI CAynn'nnrTG Cuts at: 0 463 1603 1782 Size: 463 1140 179 Fragments arranged by size: 1140 463 179 MspA1I CmG'CkG Cuts at: 0 245 296 399 1782 Size: 245 51 103 1383 Fragments arranged by size: 1383 245 103 51 NaeI GCC'GGC Cuts at: 0 382 1307 1782 Size: 382 925 475 Fragments arranged by size: 925 475 382 NarI GG'CG_CC Cuts at: 0 166 343 421 1782 Size: 166 177 78 1361 Fragments arranged by size: 1361 177 166 78 NcoI C'CATG_G Cuts at: 0 982 1782 Size: 982 800 NdeI CA'TA_TG Cuts at: 0 804 1782 Size: 804 978 NgoMIV G'CCGG_C Cuts at: 0 380 1305 1782 Size: 380 925 477 Fragments arranged by size: 925 477 380 NspI r_CATG'y Cuts at: 0 750 1608 1782 Size: 750 858 174 Fragments arranged by size: 858 750 174 PflMI CCAn_nnn'nTGG Cuts at: 0 444 1392 1782 Size: 444 948 390 Fragments arranged by size: 948 444 390 PmlI CAC'GTG Cuts at: 0 1242 1782 Size: 1242 540 PpuMI rG'GwC_Cy Cuts at: 0 816 1202 1782 Size: 816 386 580 Fragments arranged by size: 816 580 386 PshAI GACnn'nnGTC Cuts at: 0 49 1782 Size: 49 1733 PspOMI G'GGCC_C Cuts at: 0 178 448 1396 1782 Size: 178 270 948 386 Fragments arranged by size: 948 386 270 178 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 522 554 1782 Size: 522 32 1228 Fragments arranged by size: 1228 522 32 PstI C_TGCA'G Cuts at: 0 301 1740 1782 Size: 301 1439 42 Fragments arranged by size: 1439 301 42 PvuII CAG'CTG Cuts at: 0 296 1782 Size: 296 1486 SacI G_AGCT'C Cuts at: 0 908 1782 Size: 908 874 SacII CC_GC'GG Cuts at: 0 246 1782 Size: 246 1536 SapI GCTCTTCn'nnn_ Cuts at: 0 1561 1782 Size: 1561 221 ScaI AGT'ACT Cuts at: 0 475 1782 Size: 475 1307 SexAI A'CCwGG_T Cuts at: 0 437 1782 Size: 437 1345 SfcI C'TryA_G Cuts at: 0 297 1215 1736 1782 Size: 297 918 521 46 Fragments arranged by size: 918 521 297 46 SfiI GGCCn_nnn'nGGCC Cuts at: 0 245 1782 Size: 245 1537 SfoI GGC'GCC Cuts at: 0 167 344 422 1782 Size: 167 177 78 1360 Fragments arranged by size: 1360 177 167 78 SmaI CCC'GGG Cuts at: 0 265 1583 1782 Size: 265 1318 199 Fragments arranged by size: 1318 265 199 SmlI C'TyrA_G Cuts at: 0 1378 1782 Size: 1378 404 SphI G_CATG'C Cuts at: 0 1608 1782 Size: 1608 174 SspI AAT'ATT Cuts at: 0 922 1782 Size: 922 860 StuI AGG'CCT Cuts at: 0 1300 1782 Size: 1300 482 StyI C'CwwG_G Cuts at: 0 982 1336 1391 1500 1782 Size: 982 354 55 109 282 Fragments arranged by size: 982 354 282 109 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 39 1031 1278 1782 Size: 39 992 247 504 Fragments arranged by size: 992 504 247 39 TatI w'GTAC_w Cuts at: 0 473 523 1782 Size: 473 50 1259 Fragments arranged by size: 1259 473 50 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 196 228 736 768 1782 Size: 196 32 508 32 1014 Fragments arranged by size: 1014 508 196 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1672 1782 Size: 1672 110 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1298 1782 Size: 1298 484 XmaI C'CCGG_G Cuts at: 0 263 1581 1782 Size: 263 1318 201 Fragments arranged by size: 1318 263 201 Enzymes that do cut: AarI Acc65I AcuI AleI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PflMI PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaBI BsiWI BsmBI BspHI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoRI EcoRV FspAI HindIII HpaI MfeI MluI MmeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PsiI PspXI PvuI RsrII SalI SanDI SbfI SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI XmnI ZraI