Restriction map for pFN21AE6134
 (Circular) (Six-base) MAPSORT of: pFN21AE6134.seq  Check: 8737  from: 1  to: 5134



pFN21AE6134  5134 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:53  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2944     278 
   Size:         53      83     186     936    1408    2468

  Fragments arranged by size: 

                2468    1408     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2477    1937 
   Size:        540    4594
 
Acc65I G'GTAC_C

Cuts at:   2455    2455 
   Size:       5134
 
AclI AA'CG_TT

Cuts at:   1563    3261    3634    1563 
   Size:       1698     373    3063

  Fragments arranged by size: 

                3063    1698     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3197    4581    4849    1632 
   Size:       1565    1384     268    1917

  Fragments arranged by size: 

                1917    1565    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4937
 
AflIII A'CryG_T

Cuts at:   1203    4033    5010    1203 
   Size:       2830     977    1327

  Fragments arranged by size: 

                2830    1327     977
 
AgeI A'CCGG_T

Cuts at:   3965    3965 
   Size:       5134
 
AhdI GACnn_n'nnGTC

Cuts at:   3863    3863 
   Size:       5134
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5134
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5134
 
AlwNI CAG_nnn'CTG

Cuts at:   2167    2877    4449    2167 
   Size:        710    1572    2852

  Fragments arranged by size: 

                2852    1572     710
 
ApaLI G'TGCA_C

Cuts at:   3191    4347    3191 
   Size:       1156    3978
 
ApoI r'AATT_y

Cuts at:   1493    2443    2669    2821    4930    1493 
   Size:        950     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     950     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3688     160 
   Size:        872    2656    1606

  Fragments arranged by size: 

                2656    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5134
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2459    1870 
   Size:         73     516    4545

  Fragments arranged by size: 

                4545     516      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2022    2055    2022 
   Size:         33    5101
 
BamHI G'GATC_C

Cuts at:   2464    2831    2464 
   Size:        367    4767
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2455    3910     618 
   Size:        325     217     627     668    1455    1842

  Fragments arranged by size: 

                1842    1455     668     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2390    2453    1391 
   Size:        999      63    4072

  Fragments arranged by size: 

                4072     999      63
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4163
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3325    3359    1903 
   Size:         34    1388      34    3678

  Fragments arranged by size: 

                3678    1388      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3026    4236    3026 
   Size:       1210    3924
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5134
 
BfrBI ATG'CAT

Cuts at:   2014    2014 
   Size:       5134
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3745     136 
   Size:        107     122      71     631     605    2073    1525

  Fragments arranged by size: 

                2073    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5129    5129 
   Size:       5134
 
BlpI GC'TnA_GC

Cuts at:   2551    2551 
   Size:       5134
 
Bme1580I G_kGCm'C

Cuts at:   1790    3195    4351    1790 
   Size:       1405    1156    2573

  Fragments arranged by size: 

                2573    1405    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5134
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2865    3823     442 
   Size:        416      53      83     399      24    1448     958    1753

  Fragments arranged by size: 

                1753    1448     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5134
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2077    3794    1239 
   Size:        838    1717    2579

  Fragments arranged by size: 

                2579    1717     838
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3259    4124    4422    3259 
   Size:        865     298    3971

  Fragments arranged by size: 

                3971     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3797     882 
   Size:        617    2298    2219

  Fragments arranged by size: 

                2298    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2925     493 
   Size:        711    1721    2702

  Fragments arranged by size: 

                2702    1721     711
 
BsaBI GATnn'nnATC

Cuts at:   5128    5128 
   Size:       5134
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2941    3323    3958     275 
   Size:         53      83     186     936    1408     382     635    1451

  Fragments arranged by size: 

                1451    1408     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3567    3965    4239    4386    4797    3567 
   Size:        398     274     147     411    3904

  Fragments arranged by size: 

                3904     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4909

  Fragments arranged by size: 

                4909     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5134
 
BseYI C'CCAG_C

Cuts at:   4337    4833    4337 
   Size:        496    4638
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4561
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2584    3345    4373    1540 
   Size:        275     186     583     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     583     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2103    3195    3280    4351    2103 
   Size:       1092      85    1071    2886

  Fragments arranged by size: 

                2886    1092    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2645    2738    2645 
   Size:         93    5041
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2103    2390    2453    3195    3280    4351    1391 
   Size:        399     313     287      63     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     313     287      85      63
            
 
BspEI T'CCGG_A

Cuts at:   4797    4797 
   Size:       5134
 
BspHI T'CATG_A

Cuts at:   3023    4753    3023 
   Size:       1730    3404
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2184    2489     844 
   Size:       1340     305    3489

  Fragments arranged by size: 

                3489    1340     305
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3021    1394 
   Size:        429    1198    3507

  Fragments arranged by size: 

                3507    1198     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3629    3803      65 
   Size:       1183    2381     174    1396

  Fragments arranged by size: 

                2381    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3778    3965    1700 
   Size:        110    1968     187    2869

  Fragments arranged by size: 

                2869    1968     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5134
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5134
 
BssSI C'ACGA_G

Cuts at:   3194    4206    3194 
   Size:       1012    4122
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5134
 
BstBI TT'CG_AA

Cuts at:   3938    3938 
   Size:       5134
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5134
 
BstYI r'GATC_y

Cuts at:   1972    2464    2831    3216    3233    4685    4771    4783    5129 
   Size:        492     367     385      17    1452      86      12     346

Cuts at:   5129    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     492     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3643

  Fragments arranged by size: 

                3643     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2024    2188     487 
   Size:        683      56     798     164    3433

  Fragments arranged by size: 

                3433     798     683     164      56
 
BtsI GCAGTG_nn'

Cuts at:   2650    3444    3464    2650 
   Size:        794      20    4320

  Fragments arranged by size: 

                4320     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5134
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5099
 
DraI TTT'AAA

Cuts at:   2438    2438 
   Size:       5134
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4141     809 
   Size:        507    2825    1802

  Fragments arranged by size: 

                2825    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2287    2340    2581 
   Size:         54    1425      50     275      18     457      53     241

Cuts at:   2581    3470    5099       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     457     275     241      54      53
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2581    1537 
   Size:        275     769    4090

  Fragments arranged by size: 

                4090     769     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3064    1353 
   Size:       1711    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3721    4095    4241     387 
   Size:       1064     138    2132     374     146    1280

  Fragments arranged by size: 

                2132    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2077    3197    3794    4581    4849    1239 
   Size:        393     445    1120     597     787     268    1524

  Fragments arranged by size: 

                1524    1120     787     597     445     393     268
 
EcoRI G'AATT_C

Cuts at:   2443    2821    2443 
   Size:        378    4756
 
FspI TGC'GCA

Cuts at:   3640    3640 
   Size:       5134
 
HaeII r_GCGC'y

Cuts at:   1678    4281    1678 
   Size:       2603    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2207    2239    3775    3807    3849 
   Size:         32     135      32     635      32    1536      32      42

Cuts at:   3849    3881    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1536     635     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2478    2724     669 
   Size:        988     281     540     246    3079

  Fragments arranged by size: 

                3079     988     540     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5134
 
HpaI GTT'AAC

Cuts at:   2724    2724 
   Size:       5134
 
KpnI G_GTAC'C

Cuts at:   2459    2459 
   Size:       5134
 
MfeI C'AATT_G

Cuts at:   2733    2733 
   Size:       5134
 
MluI A'CGCG_T

Cuts at:   5010    5010 
   Size:       5134
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4248    4432    1305 
   Size:       2943     184    2007

  Fragments arranged by size: 

                2943    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2289      10 
   Size:         54    1425     343     457    2855

  Fragments arranged by size: 

                2855    1425     457     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    2312    3092    3451    3610     518 
   Size:        648     843     303     780     359     159    2042

  Fragments arranged by size: 

                2042     843     780     648     359     303     159
 
MspA1I CmG'CkG

Cuts at:   1606    2036    2432    2550    3227    4375    4620    1606 
   Size:        430     396     118     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     430     396     245     118
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3643

  Fragments arranged by size: 

                3643     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5134
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5134
 
NotI GC'GGCC_GC

Cuts at:   2581    2581 
   Size:       5134
 
NsiI A_TGCA'T

Cuts at:   2016    2016 
   Size:       5134
 
NspI r_CATG'y

Cuts at:   1132    1229    2492    4037    1132 
   Size:         97    1263    1545    2229

  Fragments arranged by size: 

                2229    1545    1263      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5134
 
PciI A'CATG_T

Cuts at:   4033    4033 
   Size:       5134
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2167    1108 
   Size:       1059    4075
 
PmeI GTTT'AAAC

Cuts at:   2438    2438 
   Size:       5134
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3203    3235    4716    4748    3203 
   Size:         32    1481      32    3589

  Fragments arranged by size: 

                3589    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5134
 
PsiI TTA'TAA

Cuts at:   2704    2704 
   Size:       5134
 
PstI C_TGCA'G

Cuts at:    830    2230    2486     830 
   Size:       1400     256    3478

  Fragments arranged by size: 

                3478    1400     256
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5134
 
PvuII CAG'CTG

Cuts at:   2432    2432 
   Size:       5134
 
SalI G'TCGA_C

Cuts at:   1936    2476    1936 
   Size:        540    4594
 
SbfI CC_TGCA'GG

Cuts at:   2486    2486 
   Size:       5134
 
ScaI AGT'ACT

Cuts at:   3382    5094    3382 
   Size:       1712    3422
 
SexAI A'CCwGG_T

Cuts at:   2160    2160 
   Size:       5134
 
SfcI C'TryA_G

Cuts at:    826    1045    2226    2482    3617    4298    4489     826 
   Size:        219    1181     256    1135     681     191    1471

  Fragments arranged by size: 

                1471    1181    1135     681     256     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5134
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5134
 
SmaI CCC'GGG

Cuts at:   1872    2461    1872 
   Size:        589    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3238    4139    4401     820 
   Size:        197     926    1295     901     262    1553

  Fragments arranged by size: 

                1553    1295     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2925     493 
   Size:       2432    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5134
 
SphI G_CATG'C

Cuts at:   1132    2492    1132 
   Size:       1360    3774
 
SspI AAT'ATT

Cuts at:      5      52    3058       5 
   Size:         47    3006    2081

  Fragments arranged by size: 

                3006    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5134
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2371    2573     513 
   Size:        552     939     367     202    3074

  Fragments arranged by size: 

                3074     939     552     367     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3161    3178    3331    3516    3961     325 
   Size:       1255    1581      17     153     185     445    1498

  Fragments arranged by size: 

                1581    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2116    3380    5092 
   Size:        274      80      33      51    1443     139    1264    1712

Cuts at:   5092      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1264     274     139     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5094    5126    5094 
   Size:         32    5102
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5134
 
XbaI T'CTAG_A

Cuts at:   2470    2470 
   Size:       5134
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2072    2072 
   Size:       5134
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5134
 
XmaI C'CCGG_G

Cuts at:   1870    2459    1870 
   Size:        589    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3263    3263 
   Size:       5134
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2942     276 
   Size:         53      83     186     936    1408    2468

  Fragments arranged by size: 

                2468    1408     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI
     SfiI     SfoI     SrfI     SwaI
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