################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AspHI AviII BbeI BbrPI BbvCI BciVI BfrI BglII BsaAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KspI MfeI MluI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SfoI SfuI SgrAI SnaBI SpeI SspBI StuI SwaI TfiI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 802 GACGTC GACGT^C Acc65I 1457 GGTACC G^GTACC ApaI 767 GGGCCC GGGCC^C Asp700I 1041 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1457 GGTACC G^GTACC AspI 841 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1017 CCTAGG C^CTAGG BalI 452 TGGCCA TGG^CCA BamHI 494 GGATCC G^GATCC BanI 1457 GGTACC G^G[CT][GA]CC BbsI 1534 GTCTTC GAAGAC(2/6) BclI 320 TGATCA T^GATCA BlnI 1017 CCTAGG C^CTAGG BpuAI 1534 GTCTTC GAAGAC(2/6) BsaBI 1582 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 1700 TCATGA T^CATGA BssSI 370 CACGAG CACGAG(-5/-1) DraIII 594 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 960 GAAGAG CTCTTC(1/4) FbaI 320 TGATCA T^GATCA KpnI 1457 GGTACC GGTAC^C Ksp632I 960 GAAGAG CTCTTC(1/4) MamI 1582 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 452 TGGCCA TGG^CCA MscI 452 TGGCCA TGG^CCA NspI 1263 GCATGC [GA]CATG^[CT] PflFI 841 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1013 GGGACCT [GA]G^G[AT]CC[CT] RcaI 1700 TCATGA T^CATGA SbfI 498 CCTGCAGG CCTGCA^GG SexAI 1649 ACCTGGT A^CC[AT]GGT SfiI 123 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 310 CTCAAG C^T[CT][GA]AG SphI 1263 GCATGC GCATG^C Sse8387I 498 CCTGCAGG CCTGCA^GG SspI 616 AATATT AAT^ATT Tth111I 841 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1041 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccIII 572 TCCGGA T^CCGGA 906 TCCGGA AcyI 730 GGCGTC G[GA]^CG[CT]C 802 GACGTC BcgI 372 CGAGTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 876 CGACGTGGCTGC BfaI 676 CTAG C^TAG 1018 CTAG BlpI 1436 GCTCAGC GC^T[ACGT]AGC 1710 GCTAAGC Bpu1102I 1436 GCTCAGC GC^T[ACGT]AGC 1710 GCTAAGC BsaHI 730 GGCGTC G[GA]^CG[CT]C 802 GACGTC BseAI 572 TCCGGA T^CCGGA 906 TCCGGA BseRI 1315 GAGGAG GAGGAG(10/8) 1529 GAGGAG BspEI 572 TCCGGA T^CCGGA 906 TCCGGA BspMI 812 ACCTGC ACCTGC(4/8) 424 GCAGGT BsrBI 1434 CCGCTC CCGCTC(-3/-3) 1491 CCGCTC BstAPI 174 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1474 GCAGTCATTGC BstEII 708 GGTGACC G^GT[ACGT]ACC 1359 GGTCACC BstPI 708 GGTGACC G^GT[ACGT]ACC 1359 GGTCACC BstXI 455 CCATCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 872 CCAGCGACGTGG CelII 1436 GCTCAGC GC^T[ACGT]AGC 1710 GCTAAGC DraII 766 GGGGCCC [GA]G^G[ACGT]CC[CT] 1013 GGGACCT EcoO109I 766 GGGGCCC [GA]G^G[ACGT]CC[CT] 1013 GGGACCT EcoO65I 708 GGTGACC G^GT[ACGT]ACC 1359 GGTCACC HgaI 576 GACGC GACGC(5/10) 731 GCGTC Hin1I 730 GGCGTC G[GA]^CG[CT]C 802 GACGTC MaeI 676 CTAG C^TAG 1018 CTAG MroI 572 TCCGGA T^CCGGA 906 TCCGGA PflMI 144 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 543 CCAGGTGCTGG PstI 28 CTGCAG CTGCA^G 499 CTGCAG SfcI 28 CTGCAG C^T[GA][CT]AG 499 CTGCAG SmaI 784 CCCGGG CCC^GGG 1274 CCCGGG TaqI 4 TCGA T^CGA 1025 TCGA Tsp509I 939 AATT ^AATT 1601 AATT TthHB8I 4 TCGA T^CGA 1025 TCGA Van91I 144 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 543 CCAGGTGCTGG XmaCI 784 CCCGGG C^CCGGG 1274 CCCGGG XmaI 784 CCCGGG C^CCGGG 1274 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BsaWI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI XcmI XhoII