(Linear) (Six-base) MAPSORT of: KIEE1326.seq Check: 648 from: 1 to: 1722 KIEE1326.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AatII G_ACGT'C Cuts at: 0 806 1722 Size: 806 916 Acc65I G'GTAC_C Cuts at: 0 1457 1722 Size: 1457 265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1286 1722 Size: 1286 436 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 7 39 553 585 1722 Size: 7 32 514 32 1137 Fragments arranged by size: 1137 514 32 32 7 AlwNI CAG_nnn'CTG Cuts at: 0 150 180 549 1119 1449 1722 Size: 150 30 369 570 330 273 Fragments arranged by size: 570 369 330 273 150 30 ApaI G_GGCC'C Cuts at: 0 771 1722 Size: 771 951 AvaI C'yCGr_G Cuts at: 0 784 1274 1466 1722 Size: 784 490 192 256 Fragments arranged by size: 784 490 256 192 AvrII C'CTAG_G Cuts at: 0 1017 1722 Size: 1017 705 BamHI G'GATC_C Cuts at: 0 494 1722 Size: 494 1228 BanI G'GyrC_C Cuts at: 0 1457 1722 Size: 1457 265 BanII G_rGCy'C Cuts at: 0 93 245 541 668 771 1722 Size: 93 152 296 127 103 951 Fragments arranged by size: 951 296 152 127 103 93 BbsI GAAGACnn'nnnn_ Cuts at: 0 1527 1722 Size: 1527 195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 361 395 865 899 1722 Size: 361 34 470 34 823 Fragments arranged by size: 823 470 361 34 34 BclI T'GATC_A Cuts at: 0 320 1722 Size: 320 1402 BglI GCCn_nnn'nGGC Cuts at: 0 130 749 892 1279 1573 1722 Size: 130 619 143 387 294 149 Fragments arranged by size: 619 387 294 149 143 130 BlpI GC'TnA_GC Cuts at: 0 1437 1711 1722 Size: 1437 274 11 Fragments arranged by size: 1437 274 11 Bme1580I G_kGCm'C Cuts at: 0 157 771 1722 Size: 157 614 951 Fragments arranged by size: 951 614 157 BmgBI CAC'GTC Cuts at: 0 879 1722 Size: 879 843 BmrI ACTGGGnnnn_n' Cuts at: 0 500 1722 Size: 500 1222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 1000 1141 1544 1722 Size: 77 923 141 403 178 Fragments arranged by size: 923 403 178 141 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 1722 Size: 295 1427 BsaI GGTCTCn'nnnn_ Cuts at: 0 51 803 1640 1722 Size: 51 752 837 82 Fragments arranged by size: 837 752 82 51 BsaBI GATnn'nnATC Cuts at: 0 1586 1722 Size: 1586 136 BsaHI Gr'CG_yC Cuts at: 0 731 803 1722 Size: 731 72 919 Fragments arranged by size: 919 731 72 BsaWI w'CCGG_w Cuts at: 0 477 572 906 1722 Size: 477 95 334 816 Fragments arranged by size: 816 477 334 95 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 220 250 1637 1667 1722 Size: 220 30 1387 30 55 Fragments arranged by size: 1387 220 55 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1330 1544 1722 Size: 1330 214 178 Fragments arranged by size: 1330 214 178 BseYI C'CCAG_C Cuts at: 0 150 1032 1442 1722 Size: 150 882 410 280 Fragments arranged by size: 882 410 280 150 Bsp1286I G_dGCh'C Cuts at: 0 93 157 245 541 668 771 1722 Size: 93 64 88 296 127 103 951 Fragments arranged by size: 951 296 127 103 93 88 64 BspEI T'CCGG_A Cuts at: 0 572 906 1722 Size: 572 334 816 Fragments arranged by size: 816 572 334 BspHI T'CATG_A Cuts at: 0 1700 1722 Size: 1700 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 415 821 1722 Size: 415 406 901 Fragments arranged by size: 901 415 406 BsrBI CCG'CTC Cuts at: 0 1436 1493 1722 Size: 1436 57 229 Fragments arranged by size: 1436 229 57 BsrDI GCAATG_nn' Cuts at: 0 269 338 1478 1722 Size: 269 69 1140 244 Fragments arranged by size: 1140 269 244 69 BsrFI r'CCGG_y Cuts at: 0 443 633 1301 1722 Size: 443 190 668 421 Fragments arranged by size: 668 443 421 190 BssSI C'ACGA_G Cuts at: 0 370 1722 Size: 370 1352 BstAPI GCAn_nnn'nTGC Cuts at: 0 180 1480 1722 Size: 180 1300 242 Fragments arranged by size: 1300 242 180 BstEII G'GTnAC_C Cuts at: 0 708 1359 1722 Size: 708 651 363 Fragments arranged by size: 708 651 363 BstXI CCAn_nnnn'nTGG Cuts at: 0 462 879 1722 Size: 462 417 843 Fragments arranged by size: 843 462 417 BstYI r'GATC_y Cuts at: 0 482 494 1028 1722 Size: 482 12 534 694 Fragments arranged by size: 694 534 482 12 BtgI C'CryG_G Cuts at: 0 245 299 360 449 1626 1722 Size: 245 54 61 89 1177 96 Fragments arranged by size: 1177 245 96 89 61 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 375 441 1181 1722 Size: 375 66 740 541 Fragments arranged by size: 740 541 375 66 BtsI GCAGTG_nn' Cuts at: 0 155 1298 1722 Size: 155 1143 424 Fragments arranged by size: 1143 424 155 DraIII CAC_nnn'GTG Cuts at: 0 599 1722 Size: 599 1123 EaeI y'GGCC_r Cuts at: 0 452 1144 1431 1722 Size: 452 692 287 291 Fragments arranged by size: 692 452 291 287 EarI CTCTTCn'nnn_ Cuts at: 0 955 1722 Size: 955 767 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 1000 1141 1544 1722 Size: 77 923 141 403 178 Fragments arranged by size: 923 403 178 141 77 EcoO109I rG'GnC_Cy Cuts at: 0 767 1014 1722 Size: 767 247 708 Fragments arranged by size: 767 708 247 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1251 1722 Size: 1251 471 HaeII r_GCGC'y Cuts at: 0 309 1077 1336 1722 Size: 309 768 259 386 Fragments arranged by size: 768 386 309 259 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 4 36 75 107 220 252 467 499 Size: 4 32 39 32 113 32 215 32 Cuts at: 499 905 937 1722 Size: 406 32 785 Fragments arranged by size: 785 406 215 113 39 32 32 32 32 32 4 KpnI G_GTAC'C Cuts at: 0 1461 1722 Size: 1461 261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 497 1623 1722 Size: 497 1126 99 Fragments arranged by size: 1126 497 99 MscI TGG'CCA Cuts at: 0 454 1722 Size: 454 1268 MslI CAynn'nnrTG Cuts at: 0 1000 1114 1414 1705 1722 Size: 1000 114 300 291 17 Fragments arranged by size: 1000 300 291 114 17 MspA1I CmG'CkG Cuts at: 0 106 180 187 1308 1722 Size: 106 74 7 1121 414 Fragments arranged by size: 1121 414 106 74 7 NspI r_CATG'y Cuts at: 0 1267 1722 Size: 1267 455 PflMI CCAn_nnn'nTGG Cuts at: 0 150 549 1722 Size: 150 399 1173 Fragments arranged by size: 1173 399 150 PfoI T'CCnGG_A Cuts at: 0 8 93 206 401 977 1722 Size: 8 85 113 195 576 745 Fragments arranged by size: 745 576 195 113 85 8 PpuMI rG'GwC_Cy Cuts at: 0 1014 1722 Size: 1014 708 PspOMI G'GGCC_C Cuts at: 0 767 1722 Size: 767 955 PstI C_TGCA'G Cuts at: 0 32 503 1722 Size: 32 471 1219 Fragments arranged by size: 1219 471 32 PvuII CAG'CTG Cuts at: 0 180 187 1308 1722 Size: 180 7 1121 414 Fragments arranged by size: 1121 414 180 7 SbfI CC_TGCA'GG Cuts at: 0 503 1722 Size: 503 1219 SexAI A'CCwGG_T Cuts at: 0 1649 1722 Size: 1649 73 SfcI C'TryA_G Cuts at: 0 28 499 1722 Size: 28 471 1223 Fragments arranged by size: 1223 471 28 SfiI GGCCn_nnn'nGGCC Cuts at: 0 130 1722 Size: 130 1592 SmaI CCC'GGG Cuts at: 0 786 1276 1722 Size: 786 490 446 Fragments arranged by size: 786 490 446 SmlI C'TyrA_G Cuts at: 0 310 1722 Size: 310 1412 SphI G_CATG'C Cuts at: 0 1267 1722 Size: 1267 455 SrfI GCCC'GGGC Cuts at: 0 786 1722 Size: 786 936 SspI AAT'ATT Cuts at: 0 618 1722 Size: 618 1104 StyI C'CwwG_G Cuts at: 0 470 1017 1226 1722 Size: 470 547 209 496 Fragments arranged by size: 547 496 470 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 26 591 1722 Size: 26 565 1131 Fragments arranged by size: 1131 565 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1637 1669 1722 Size: 1637 32 53 Fragments arranged by size: 1637 53 32 Tth111I GACn'n_nGTC Cuts at: 0 844 1722 Size: 844 878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1116 1182 1374 1568 1722 Size: 1116 66 192 194 154 Fragments arranged by size: 1116 194 192 154 66 XmaI C'CCGG_G Cuts at: 0 784 1274 1722 Size: 784 490 448 Fragments arranged by size: 784 490 448 XmnI GAAnn'nnTTC Cuts at: 0 1045 1722 Size: 1045 677 ZraI GAC'GTC Cuts at: 0 804 1722 Size: 804 918 Enzymes that do cut: AatII Acc65I AlfI AloI AlwNI ApaI AvaI AvrII BamHI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NspI PflMI PfoI PpuMI PspOMI PstI PvuII SbfI SexAI SfcI SfiI SmaI SmlI SphI SrfI SspI StyI TaqII TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI BaeI BbeI BbvCI BciVI BfrBI BglII BmtI BplI Bpu10I BsaAI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SfoI SgfI SgrAI SnaBI SpeI StuI SwaI TatI XbaI XhoI