Restriction map for
pFN21AE1344
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BcnI
BglI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 891 GTAC GT^AC
AlwNI 1096 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 825 GGGCCC GGGCC^C
AvaI 59 CTCGGG C^[CT]CG[GA]G
AvrII 982 CCTAGG C^CTAGG
BalI 907 TGGCCA TGG^CCA
BciVI 763 GTATCC GTATCC(6/5)
BclI 1412 TGATCA T^GATCA
BglII 1385 AGATCT A^GATCT
BlnI 982 CCTAGG C^CTAGG
BlpI 915 GCTAAGC GC^T[ACGT]AGC
BpmI 84 CTCCAG CTGGAG(16/14)
Bpu1102I 915 GCTAAGC GC^T[ACGT]AGC
BseRI 134 CTCCTC GAGGAG(10/8)
BsoBI 59 CTCGGG C^[CT]CG[GA]G
Bsp1407I 890 TGTACA T^GTACA
BspMI 1488 GCAGGT ACCTGC(4/8)
BsrGI 890 TGTACA T^GTACA
BssSI 137 CTCGTG CACGAG(-5/-1)
CelII 915 GCTAAGC GC^T[ACGT]AGC
DraI 676 TTTAAA TTT^AAA
DraIII 928 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 306 GACAATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 910 CCATGG C^C[GA][CT]GG
EaeI 907 TGGCCA [CT]^GGCC[GA]
EcoNI 654 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 974 GAATTC G^AATTC
FauI 1130 CCCGC CCCGC(4/6)
FbaI 1412 TGATCA T^GATCA
HapII 639 CCGG C^CGG
HindIII 1643 AAGCTT A^AGCTT
HpaII 639 CCGG C^CGG
MaeII 171 ACGT A^CGT
MluNI 907 TGGCCA TGG^CCA
MscI 907 TGGCCA TGG^CCA
MspI 639 CCGG C^CGG
MwoI 474 GCAAGCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 910 CCATGG C^CATGG
NdeI 808 CATATG CA^TATG
PleI 1204 GAGTC GAGTC(4/5)
RsaI 891 GTAC GT^AC
SspBI 890 TGTACA T^GTACA
TaqI 1071 TCGA T^CGA
TthHB8I 1071 TCGA T^CGA
XbaI 1087 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 1131 CCGC CCGC(-3/-1)
1495 GCGG
AflII 271 CTTAAG C^TTAAG
633 CTTAAG
AspHI 605 GTGCTC G[AT]GC[AT]^C
1355 GAGCAC
BanII 825 GGGCCC G[GA]GC[CT]^C
1531 GGGCTC
BbvI 513 GCTGC GCAGC(8/12)
1111 GCTGC
BfrI 271 CTTAAG C^TTAAG
633 CTTAAG
BsiHKAI 605 GTGCTC G[AT]GC[AT]^C
1355 GAGCAC
BstYI 960 GGATCT [GA]^GATC[CT]
1385 AGATCT
DraII 824 AGGGCCC [GA]G^G[ACGT]CC[CT]
825 GGGCCCC
EcoO109I 824 AGGGCCC [GA]G^G[ACGT]CC[CT]
825 GGGCCCC
EcoT14I 910 CCATGG C^C[AT][AT]GG
982 CCTAGG
HphI 345 TCACC GGTGA(8/7)
1255 TCACC
MflI 960 GGATCT [GA]^GATC[CT]
1385 AGATCT
MslI 565 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
831 CATGATTGTG
PstI 815 CTGCAG CTGCA^G
1112 CTGCAG
SfaNI 91 GATGC GCATC(5/9)
1404 GATGC
StyI 910 CCATGG C^C[AT][AT]GG
982 CCTAGG
TseI 513 GCTGC G^C[AT]GC
1111 GCTGC
XcmI 164 CCAGGAAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
830 CCATGATTGTGTTGG
XhoII 960 GGATCT [GA]^GATC[CT]
1385 AGATCT
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI NdeII NlaIII NlaIV
NspI PvuII Sau3AI Sau96I ScrFI
SfcI SmlI TfiI Tru9I Tsp45I
Tsp509I TspRI