################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BanI BbeI BcgI BciVI BfrI BlnI BsiEI BsiWI BsmBI BsmI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CfoI Cfr10I CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI HinP1I HindIII HpaI KasI KpnI KspI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 1411 GACGTC GACGT^C AcyI 1411 GACGTC G[GA]^CG[CT]C AflIII 905 ACACGT A^C[GA][CT]GT Alw44I 765 GTGCAC G^TGCAC AlwNI 214 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 14 GGGCCC GGGCC^C ApaLI 765 GTGCAC G^TGCAC AspI 1999 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 548 CTCGAG C^[CT]CG[GA]G BalI 878 TGGCCA TGG^CCA BamHI 638 GGATCC G^GATCC BbrPI 906 CACGTG CAC^GTG BbsI 1408 GAAGAC GAAGAC(2/6) BcnI 1494 CCCGG CC^[GC]GG BglII 2106 AGATCT A^GATCT BlpI 748 GCTCAGC GC^T[ACGT]AGC Bpu1102I 748 GCTCAGC GC^T[ACGT]AGC BpuAI 1408 GAAGAC GAAGAC(2/6) BsaAI 906 CACGTG [CT]AC^GT[GA] BsaBI 1512 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1411 GACGTC G[GA]^CG[CT]C BsaI 551 GAGACC GGTCTC(1/5) BseRI 255 CTCCTC GAGGAG(10/8) BsoBI 548 CTCGAG C^[CT]CG[GA]G Bsp1407I 2135 TGTACA T^GTACA BspDI 1296 ATCGAT AT^CGAT BspMI 1162 GCAGGT ACCTGC(4/8) BsrGI 2135 TGTACA T^GTACA BstAPI 210 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 678 GGTCACC G^GT[ACGT]ACC BstPI 678 GGTCACC G^GT[ACGT]ACC CelII 748 GCTCAGC GC^T[ACGT]AGC ClaI 1296 ATCGAT AT^CGAT DraI 1332 TTTAAA TTT^AAA DraII 473 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 27 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1171 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 786 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 473 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 678 GGTCACC G^GT[ACGT]ACC Hin1I 1411 GACGTC G[GA]^CG[CT]C MamI 1512 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 491 CAATTG C^AATTG MluNI 878 TGGCCA TGG^CCA MscI 878 TGGCCA TGG^CCA MspA1I 2090 CAGCTG C[AC]G^C[GT]G MunI 491 CAATTG C^AATTG NciI 1494 CCCGG CC^[GC]GG NdeI 643 CATATG CA^TATG PaeR7I 548 CTCGAG C^TCGAG PflFI 1999 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 906 CACGTG CAC^GTG PmlI 906 CACGTG CAC^GTG PvuII 2090 CAGCTG CAG^CTG SacI 746 GAGCTC GAGCT^C SexAI 368 ACCAGGT A^CC[AT]GGT SfiI 15 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 2135 TGTACA T^GTACA Tth111I 1999 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2185 TCTAGA T^CTAGA XhoI 548 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 174 CGCG CG^CG 598 CGCG AccIII 296 TCCGGA T^CCGGA 2147 TCCGGA BanII 14 GGGCCC G[GA]GC[CT]^C 746 GAGCTC BbvCI 205 CCTCAGC CCTCAGC(-5/-2) 2087 CCTCAGC BglI 16 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1654 GCCACCCAGGC BseAI 296 TCCGGA T^CCGGA 2147 TCCGGA BspEI 296 TCCGGA T^CCGGA 2147 TCCGGA BstUI 174 CGCG CG^CG 598 CGCG Cac8I 122 GCCTGC GC[ACGT]^[ACGT]GC 1917 GCCAGC EaeI 878 TGGCCA [CT]^GGCC[GA] 1856 CGGCCA EarI 117 GAAGAG CTCTTC(1/4) 264 GAAGAG EcoRI 543 GAATTC G^AATTC 574 GAATTC Ksp632I 117 GAAGAG CTCTTC(1/4) 264 GAAGAG MroI 296 TCCGGA T^CCGGA 2147 TCCGGA MvnI 174 CGCG CG^CG 598 CGCG NcoI 881 CCATGG C^CATGG 1792 CCATGG PflMI 26 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 369 CCAGGTGTTGG SapI 117 GAAGAGC GCTCTTC(1/4) 264 GAAGAGC Van91I 26 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 369 CCAGGTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BclI BfaI BmyI BpmI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII