(Circular) (Six-base) MAPSORT of: pFN21AE1317.seq Check: 9072 from: 1 to: 6919 pFN21AE1317 6919 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3420 4729 278 Size: 53 83 186 936 1884 1309 2468 Fragments arranged by size: 2468 1884 1309 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4262 1937 Size: 2325 4594 Acc65I G'GTAC_C Cuts at: 4240 4240 Size: 6919 AclI AA'CG_TT Cuts at: 1563 5046 5419 1563 Size: 3483 373 3063 Fragments arranged by size: 3483 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2316 3432 4982 6366 6634 1632 Size: 684 1116 1550 1384 268 1917 Fragments arranged by size: 1917 1550 1384 1116 684 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6722 AflIII A'CryG_T Cuts at: 1203 2910 5818 6795 1203 Size: 1707 2908 977 1327 Fragments arranged by size: 2908 1707 1327 977 AgeI A'CCGG_T Cuts at: 5750 5750 Size: 6919 AhdI GACnn_n'nnGTC Cuts at: 5648 5648 Size: 6919 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3460 1184 Size: 2276 4643 AlwNI CAG_nnn'CTG Cuts at: 2224 4662 6234 2224 Size: 2438 1572 2909 Fragments arranged by size: 2909 2438 1572 ApaI G_GGCC'C Cuts at: 2023 2023 Size: 6919 ApaLI G'TGCA_C Cuts at: 2770 4976 6132 2770 Size: 2206 1156 3557 Fragments arranged by size: 3557 2206 1156 ApoI r'AATT_y Cuts at: 1493 2548 2563 2579 4228 4454 4606 6715 1493 Size: 1055 15 16 1649 226 152 2109 1697 Fragments arranged by size: 2109 1697 1649 1055 226 152 16 15 AseI AT'TA_AT Cuts at: 160 1032 5473 160 Size: 872 4441 1606 Fragments arranged by size: 4441 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 AvaI C'yCGr_G Cuts at: 1870 1943 2553 4244 1870 Size: 73 610 1691 4545 Fragments arranged by size: 4545 1691 610 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3084 3117 3084 Size: 33 6886 BamHI G'GATC_C Cuts at: 2643 4249 4616 2643 Size: 1606 367 4946 Fragments arranged by size: 4946 1606 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4240 5695 618 Size: 325 217 627 2453 1455 1842 Fragments arranged by size: 2453 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2023 2755 4238 1391 Size: 632 732 1483 4072 Fragments arranged by size: 4072 1483 732 632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3420 928 Size: 971 1521 4427 Fragments arranged by size: 4427 1521 971 BbvCI CC'TCA_GC Cuts at: 2211 4093 2211 Size: 1882 5037 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5110 5144 1903 Size: 34 50 34 3089 34 3678 Fragments arranged by size: 3678 3089 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4811 6021 4811 Size: 1210 5709 BclI T'GATC_A Cuts at: 1548 2739 3837 3852 1548 Size: 1191 1098 15 4615 Fragments arranged by size: 4615 1191 1098 15 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 3665 5530 Size: 107 122 71 631 605 355 1638 1865 Cuts at: 5530 136 Size: 1525 Fragments arranged by size: 1865 1638 1525 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 4111 6914 4111 Size: 2803 4116 BlpI GC'TnA_GC Cuts at: 2754 4336 2754 Size: 1582 5337 Bme1580I G_kGCm'C Cuts at: 1790 2023 2397 2774 4980 6136 1790 Size: 233 374 377 2206 1156 2573 Fragments arranged by size: 2573 2206 1156 377 374 233 BmgBI CAC'GTC Cuts at: 1318 2203 2242 4066 1318 Size: 885 39 1824 4171 Fragments arranged by size: 4171 1824 885 39 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2391 3250 4650 Size: 416 53 83 399 24 974 859 1400 Cuts at: 4650 5608 442 Size: 958 1753 Fragments arranged by size: 1753 1400 974 958 859 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6919 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2774 2774 Size: 6919 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3048 3570 3733 5579 1239 Size: 1809 522 163 1846 2579 Fragments arranged by size: 2579 1846 1809 522 163 Bpu10I CC'TnA_GC Cuts at: 2211 4093 2211 Size: 1882 5037 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2099 3096 5044 5909 6207 2099 Size: 997 1948 865 298 2811 Fragments arranged by size: 2811 1948 997 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2550 5582 882 Size: 617 1051 3032 2219 Fragments arranged by size: 3032 2219 1051 617 BsaAI yAC'GTr Cuts at: 493 1204 2913 4710 493 Size: 711 1709 1797 2702 Fragments arranged by size: 2702 1797 1709 711 BsaBI GATnn'nnATC Cuts at: 3521 6913 3521 Size: 3392 3527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3417 4726 5108 5743 Size: 53 83 186 936 1884 1309 382 635 Cuts at: 5743 275 Size: 1451 Fragments arranged by size: 1884 1451 1309 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2301 2732 4152 5352 5750 6024 6171 6582 2301 Size: 431 1420 1200 398 274 147 411 2638 Fragments arranged by size: 2638 1420 1200 431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2246 2273 2276 3695 Size: 30 165 30 840 3 27 3 1419 Cuts at: 3695 3725 3731 3761 1178 Size: 30 6 30 4336 Fragments arranged by size: 4336 1419 840 165 30 30 30 30 27 6 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 1186 Size: 1065 5854 BseYI C'CCAG_C Cuts at: 3231 6122 6618 3231 Size: 2891 496 3532 Fragments arranged by size: 3532 2891 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3145 3703 4088 1163 Size: 573 1409 558 385 3994 Fragments arranged by size: 3994 1409 573 558 385 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4369 5130 6158 1540 Size: 275 186 2368 761 1028 2301 Fragments arranged by size: 2368 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2276 2755 2774 2898 3061 4980 5065 6136 2276 Size: 479 19 124 163 1919 85 1071 3059 Fragments arranged by size: 3059 1919 1071 479 163 124 85 19 BsmI GAATG_Cn' Cuts at: 4430 4523 4430 Size: 93 6826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2276 2397 2755 2774 2898 3061 Size: 399 233 253 121 358 19 124 163 Cuts at: 3061 4238 4980 5065 6136 1391 Size: 1177 742 85 1071 2174 Fragments arranged by size: 2174 1177 1071 742 399 358 253 233 163 124 121 85 19 BspEI T'CCGG_A Cuts at: 2301 4152 6582 2301 Size: 1851 2430 2638 Fragments arranged by size: 2638 2430 1851 BspHI T'CATG_A Cuts at: 4808 6538 4808 Size: 1730 5189 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3158 4274 844 Size: 2314 1116 3489 Fragments arranged by size: 3489 2314 1116 BsrBI CCG'CTC Cuts at: 1394 1823 4806 1394 Size: 429 2983 3507 Fragments arranged by size: 3507 2983 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3575 3840 3855 4029 5414 5588 65 Size: 1183 2327 265 15 174 1385 174 1396 Fragments arranged by size: 2327 1396 1385 1183 265 174 174 15 BsrFI r'CCGG_y Cuts at: 1700 1810 5563 5750 1700 Size: 110 3753 187 2869 Fragments arranged by size: 3753 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4140 96 Size: 4044 2875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6919 BssSI C'ACGA_G Cuts at: 4979 5991 4979 Size: 1012 5907 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 794 Size: 1427 5492 BstBI TT'CG_AA Cuts at: 5723 5723 Size: 6919 BstEII G'GTnAC_C Cuts at: 1184 2683 1184 Size: 1499 5420 BstYI r'GATC_y Cuts at: 1972 2304 2643 3587 3964 4111 4249 4616 5001 Size: 332 339 944 377 147 138 367 385 Cuts at: 5001 5018 6470 6556 6568 6914 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 944 385 377 367 346 339 332 147 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2886 3797 4173 513 Size: 552 1821 911 376 3259 Fragments arranged by size: 3259 1821 911 552 376 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6180 Fragments arranged by size: 6180 683 56 BtsI GCAGTG_nn' Cuts at: 2177 2361 3162 3825 4435 5229 5249 2177 Size: 184 801 663 610 794 20 3847 Fragments arranged by size: 3847 801 794 663 610 184 20 ClaI AT'CG_AT Cuts at: 1554 3302 1554 Size: 1748 5171 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3519 3554 529 Size: 35 2955 35 3894 Fragments arranged by size: 3894 2955 35 35 DraI TTT'AAA Cuts at: 3339 4223 3339 Size: 884 6035 DraIII CAC_nnn'GTG Cuts at: 2037 2037 Size: 6919 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3182 5926 809 Size: 507 1866 2744 1802 Fragments arranged by size: 2744 1866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2883 3861 4366 Size: 54 1425 50 275 18 1053 978 505 Cuts at: 4366 5255 6884 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1053 978 889 505 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4366 1537 Size: 275 2554 4090 Fragments arranged by size: 4090 2554 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2264 4849 1353 Size: 764 147 2585 3423 Fragments arranged by size: 3423 2585 764 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5506 5880 6026 387 Size: 1064 138 3917 374 146 1280 Fragments arranged by size: 3917 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2753 2753 Size: 6919 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 3048 3432 3570 3733 4982 5579 Size: 393 684 732 384 138 163 1249 597 Cuts at: 5579 6366 6634 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1249 787 732 684 597 393 384 268 163 138 EcoNI CCTnn'n_nnAGG Cuts at: 2795 2795 Size: 6919 EcoO109I rG'GnC_Cy Cuts at: 2479 2479 Size: 6919 EcoRI G'AATT_C Cuts at: 2548 2579 4228 4606 2548 Size: 31 1649 378 4861 Fragments arranged by size: 4861 1649 378 31 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4223 4223 Size: 6919 FspI TGC'GCA Cuts at: 5425 5425 Size: 6919 HaeII r_GCGC'y Cuts at: 1678 6066 1678 Size: 4388 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3267 3299 3829 3861 3944 Size: 32 135 32 1695 32 530 32 83 Cuts at: 3944 3976 5560 5592 5634 5666 1373 Size: 32 1584 32 42 32 2626 Fragments arranged by size: 2626 1695 1584 530 135 83 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2116 2455 2962 4263 4509 669 Size: 988 281 178 339 507 1301 246 3079 Fragments arranged by size: 3079 1301 988 507 339 281 246 178 HindIII A'AGCT_T Cuts at: 748 748 Size: 6919 HpaI GTT'AAC Cuts at: 4509 4509 Size: 6919 KpnI G_GTAC'C Cuts at: 4244 4244 Size: 6919 MfeI C'AATT_G Cuts at: 2496 4518 2496 Size: 2022 4897 MluI A'CGCG_T Cuts at: 6795 6795 Size: 6919 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3120 3969 3991 6033 6217 1305 Size: 1815 849 22 2042 184 2007 Fragments arranged by size: 2042 2007 1815 849 184 22 MscI TGG'CCA Cuts at: 10 64 1489 1832 2885 10 Size: 54 1425 343 1053 4044 Fragments arranged by size: 4044 1425 1053 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2326 2735 2780 2885 3833 4877 5236 Size: 648 1160 409 45 105 948 1044 359 Cuts at: 5236 5395 518 Size: 159 2042 Fragments arranged by size: 2042 1160 1044 948 648 409 359 159 105 45 MspA1I CmG'CkG Cuts at: 1606 4097 4335 5012 6160 6405 1606 Size: 2491 238 677 1148 245 2120 Fragments arranged by size: 2491 2120 1148 677 245 238 NcoI C'CATG_G Cuts at: 513 1065 2886 3797 513 Size: 552 1821 911 3635 Fragments arranged by size: 3635 1821 911 552 NdeI CA'TA_TG Cuts at: 387 2649 387 Size: 2262 4657 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6919 NotI GC'GGCC_GC Cuts at: 4366 4366 Size: 6919 NspI r_CATG'y Cuts at: 1132 1229 4277 5822 1132 Size: 97 3048 1545 2229 Fragments arranged by size: 3048 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2403 1348 Size: 1055 5864 PciI A'CATG_T Cuts at: 5818 5818 Size: 6919 PflMI CCAn_nnn'nTGG Cuts at: 1108 2037 2380 1108 Size: 929 343 5647 Fragments arranged by size: 5647 929 343 PmeI GTTT'AAAC Cuts at: 4223 4223 Size: 6919 PmlI CAC'GTG Cuts at: 2913 2913 Size: 6919 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4988 5020 6501 6533 4988 Size: 32 1481 32 5374 Fragments arranged by size: 5374 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6919 PsiI TTA'TAA Cuts at: 4489 4489 Size: 6919 PspOMI G'GGCC_C Cuts at: 2019 2019 Size: 6919 PstI C_TGCA'G Cuts at: 830 2172 2368 3169 4271 830 Size: 1342 196 801 1102 3478 Fragments arranged by size: 3478 1342 1102 801 196 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6919 PvuII CAG'CTG Cuts at: 4097 4097 Size: 6919 SacI G_AGCT'C Cuts at: 2755 2755 Size: 6919 SalI G'TCGA_C Cuts at: 1936 4261 1936 Size: 2325 4594 SapI GCTCTTCn'nnn_ Cuts at: 2117 2264 2117 Size: 147 6772 SbfI CC_TGCA'GG Cuts at: 4271 4271 Size: 6919 ScaI AGT'ACT Cuts at: 5167 6879 5167 Size: 1712 5207 SexAI A'CCwGG_T Cuts at: 2373 2373 Size: 6919 SfcI C'TryA_G Cuts at: 826 1045 2168 2364 3165 4267 5402 6083 6274 Size: 219 1123 196 801 1102 1135 681 191 Cuts at: 6274 826 Size: 1471 Fragments arranged by size: 1471 1135 1123 1102 801 681 219 196 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2027 2027 Size: 6919 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6919 SmaI CCC'GGG Cuts at: 1872 4246 1872 Size: 2374 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2553 3111 5023 5924 6186 Size: 197 926 135 475 558 1912 901 262 Cuts at: 6186 820 Size: 1553 Fragments arranged by size: 1912 1553 926 901 558 475 262 197 135 SnaBI TAC'GTA Cuts at: 493 4710 493 Size: 4217 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6919 SphI G_CATG'C Cuts at: 1132 4277 1132 Size: 3145 3774 SspI AAT'ATT Cuts at: 5 52 4843 5 Size: 47 4791 2081 Fragments arranged by size: 4791 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6919 StyI C'CwwG_G Cuts at: 513 1065 2023 2434 2886 2940 3019 3797 3888 Size: 552 958 411 452 54 79 778 91 Cuts at: 3888 4358 513 Size: 470 3074 Fragments arranged by size: 3074 958 778 552 470 452 411 91 79 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2725 3677 4946 4963 5116 5301 5746 Size: 1255 1145 952 1269 17 153 185 445 Cuts at: 5746 325 Size: 1498 Fragments arranged by size: 1498 1269 1255 1145 952 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2330 2871 4071 Size: 274 80 33 51 1443 353 541 1200 Cuts at: 4071 4140 5165 6877 96 Size: 69 1025 1712 138 Fragments arranged by size: 1712 1443 1200 1025 541 353 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2700 2732 6879 6911 2700 Size: 32 4147 32 2708 Fragments arranged by size: 4147 2708 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4007 1316 Size: 2691 4228 XbaI T'CTAG_A Cuts at: 4190 4255 4190 Size: 65 6854 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2410 2677 2852 2989 2410 Size: 267 175 137 6340 Fragments arranged by size: 6340 267 175 137 XhoI C'TCGA_G Cuts at: 1943 2553 1943 Size: 610 6309 XmaI C'CCGG_G Cuts at: 1870 4244 1870 Size: 2374 4545 XmnI GAAnn'nnTTC Cuts at: 5048 5048 Size: 6919 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3418 4727 276 Size: 53 83 186 936 1884 1309 2468 Fragments arranged by size: 2468 1884 1309 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI