################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AfaI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraII DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 23 GACGTC GACGT^C AccI 148 GTCTAC GT^[AC][GT]AC AcsI 253 AAATTT [GA]^AATT[CT] AcyI 23 GACGTC G[GA]^CG[CT]C AhdI 94 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 319 AGCT AG^CT AlwI 3 GGATC GGATC(4/5) ApoI 253 AAATTT [GA]^AATT[CT] AspEI 94 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvI 54 GCTGC GCAGC(8/12) BciVI 191 GGATAC GTATCC(6/5) BsaHI 23 GACGTC G[GA]^CG[CT]C BsaI 46 GAGACC GGTCTC(1/5) BsaJI 18 CCATGG C^C[ACGT][ACGT]GG BsiYI 265 CCAAATCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 265 CCAAATCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BssKI 109 CCAGG ^CC[ACGT]GG BstNI 109 CCAGG CC^[AT]GG BstYI 3 GGATCT [GA]^GATC[CT] DsaI 18 CCATGG C^C[GA][CT]GG Eam1105I 94 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 109 CCAGG ^CC[AT]GG EcoT14I 18 CCATGG C^C[AT][AT]GG Fnu4HI 54 GCTGC GC^[ACGT]GC Hin1I 23 GACGTC G[GA]^CG[CT]C HphI 285 TCACC GGTGA(8/7) ItaI 54 GCTGC GC^[ACGT]GC MaeII 24 ACGT A^CGT MaeIII 368 GTAAC ^GT[ACGT]AC MboII 164 TCTTC GAAGA(8/7) MflI 3 GGATCT [GA]^GATC[CT] MvaI 109 CCAGG CC^[AT]GG NcoI 18 CCATGG C^CATGG NlaIII 19 CATG CATG^ PstI 200 CTGCAG CTGCA^G ScrFI 109 CCAGG CC^[ACGT]GG SfcI 200 CTGCAG C^T[GA][CT]AG SspI 142 AATATT AAT^ATT StyI 18 CCATGG C^C[AT][AT]GG TaqI 103 TCGA T^CGA TfiI 161 GAATC G^A[AT]TC TseI 54 GCTGC G^C[AT]GC TthHB8I 103 TCGA T^CGA XhoII 3 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### DpnII 4 GATC ^GATC 246 GATC DraI 136 TTTAAA TTT^AAA 291 TTTAAA MboI 4 GATC ^GATC 246 GATC MnlI 123 CCTC CCTC(7/6) 332 GAGG NdeII 4 GATC ^GATC 246 GATC PleI 100 GAGTC GAGTC(4/5) 113 GAGTC Sau3AI 4 GATC ^GATC 246 GATC SmlI 58 CTCAAG C^T[CT][GA]AG 321 CTTGAG Tsp509I 254 AATT ^AATT 341 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### BsmAI BstF5I FokI HinfI MseI Tru9I