Restriction map for
pFN21AB0949
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HinfI HpaI KpnI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp45I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 33 GTAC GT^AC
AspHI 249 GAGCTC G[AT]GC[AT]^C
BanII 249 GAGCTC G[GA]GC[CT]^C
BbsI 462 GAAGAC GAAGAC(2/6)
BciVI 431 GGATAC GTATCC(6/5)
BclI 293 TGATCA T^GATCA
BfaI 524 CTAG C^TAG
BglI 47 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 249 GAGCTC G[AGT]GC[ACT]^C
BpmI 36 CTGGAG CTGGAG(16/14)
BpuAI 462 GAAGAC GAAGAC(2/6)
BsgI 254 CTGCAC GTGCAG(16/14)
BsiHKAI 249 GAGCTC G[AT]GC[AT]^C
BsmAI 163 GTCTC GTCTC(1/5)
BsmBI 162 CGTCTC CGTCTC(1/5)
Bsp1286I 249 GAGCTC G[AGT]GC[ACT]^C
BsrBI 415 GAGCGG CCGCTC(-3/-3)
BsrDI 336 CATTGC GCAATG(2/0)
BsrFI 228 ACCGGC [GA]^CCGG[CT]
BstF5I 78 CATCC GGATG(2/0)
BstNI 330 CCTGG CC^[AT]GG
Cfr10I 228 ACCGGC [GA]^CCGG[CT]
DdeI 122 CTCAG C^T[ACGT]AG
DpnII 294 GATC ^GATC
DraI 502 TTTAAA TTT^AAA
DsaI 18 CCGCGG C^C[GA][CT]GG
EaeI 86 TGGCCG [CT]^GGCC[GA]
EcoRII 330 CCTGG ^CC[AT]GG
FbaI 293 TGATCA T^GATCA
FokI 78 CATCC GGATG(9/13)
KspI 18 CCGCGG CCGC^GG
MaeI 524 CTAG C^TAG
MaeII 161 ACGT A^CGT
MaeIII 211 GTTAC ^GT[ACGT]AC
MboI 294 GATC ^GATC
MseI 503 TTAA T^TAA
MspA1I 18 CCGCGG C[AC]G^C[GT]G
MvaI 330 CCTGG CC^[AT]GG
NdeII 294 GATC ^GATC
PstI 360 CTGCAG CTGCA^G
RsaI 33 GTAC GT^AC
SacI 249 GAGCTC GAGCT^C
SacII 18 CCGCGG CCGC^GG
Sau3AI 294 GATC ^GATC
SfcI 360 CTGCAG C^T[GA][CT]AG
Tru9I 503 TTAA T^TAA
Tsp509I 245 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 111 AGCT AG^CT
250 AGCT
BanI 3 GGCGCC G^G[CT][GA]CC
55 GGCGCC
BbeI 3 GGCGCC GGCGC^C
55 GGCGCC
BsrI 35 ACTGG ACTGG(1/-1)
208 CCAGT
Cfr13I 15 GGCCC G^G[ACGT]CC
22 GGGCC
EarI 145 GAAGAG CTCTTC(1/4)
369 GAAGAG
HgaI 27 GACGC GACGC(5/10)
218 GCGTC
HphI 276 GGTGA GGTGA(8/7)
299 TCACC
KasI 3 GGCGCC G^GCGCC
55 GGCGCC
Ksp632I 145 GAAGAG CTCTTC(1/4)
369 GAAGAG
MslI 78 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
297 CATCACCATG
NarI 3 GGCGCC GG^CGCC
55 GGCGCC
Sau96I 15 GGCCC G^G[ACGT]CC
22 GGGCC
SfoI 3 GGCGCC GGC^GCC
55 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI AcyI BbvI BcnI
BsaHI BsaJI BseRI BsiYI BslI
BssKI BstUI Cac8I CfoI FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI MboII
MnlI MspI MvnI MwoI NciI
NlaIII NlaIV ScrFI TseI