Restriction map for pFN21AE1448
 (Circular) (Six-base) MAPSORT of: pFN21AE1448.seq  Check: 7216  from: 1  to: 5794



pFN21AE1448  5794 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2020    2020 
   Size:       5794
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3604     278 
   Size:         53      83     186     936    2068    2468

  Fragments arranged by size: 

                2468    2068     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3137    1937 
   Size:       1200    4594
 
Acc65I G'GTAC_C

Cuts at:   3115    3115 
   Size:       5794
 
AclI AA'CG_TT

Cuts at:   1563    3921    4294    1563 
   Size:       2358     373    3063

  Fragments arranged by size: 

                3063    2358     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2934    3036    3857    5241    5509    1632 
   Size:       1302     102     821    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1302     821     268     102
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5597
 
AflIII A'CryG_T

Cuts at:   1203    4693    5670    1203 
   Size:       3490     977    1327

  Fragments arranged by size: 

                3490    1327     977
 
AgeI A'CCGG_T

Cuts at:   4625    4625 
   Size:       5794
 
AhdI GACnn_n'nnGTC

Cuts at:   4523    4523 
   Size:       5794
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5794
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5794
 
AlwNI CAG_nnn'CTG

Cuts at:   2722    3537    5109    2722 
   Size:        815    1572    3407

  Fragments arranged by size: 

                3407    1572     815
 
ApaI G_GGCC'C

Cuts at:   2640    2640 
   Size:       5794
 
ApaLI G'TGCA_C

Cuts at:   3851    5007    3851 
   Size:       1156    4638
 
ApoI r'AATT_y

Cuts at:   1493    2189    3103    3329    3481    5590    1493 
   Size:        696     914     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     914     696     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4348     160 
   Size:        872    3316    1606

  Fragments arranged by size: 

                3316    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5794
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2023    3119    1870 
   Size:         73      80    1096    4545

  Fragments arranged by size: 

                4545    1096      80      73
 
BamHI G'GATC_C

Cuts at:   3124    3491    3124 
   Size:        367    5427
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2008    3115    4570     618 
   Size:        325     217     627     221    1107    1455    1842

  Fragments arranged by size: 

                1842    1455    1107     627     325     221     217
 
BanII G_rGCy'C

Cuts at:   1391    2133    2640    2667    2889    3019    3113    1391 
   Size:        742     507      27     222     130      94    4072

  Fragments arranged by size: 

                4072     742     507     222     130      94      27
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2660     928 
   Size:        971     761    4062

  Fragments arranged by size: 

                4062     971     761
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3985    4019    1903 
   Size:         34    2048      34    3678

  Fragments arranged by size: 

                3678    2048      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3686    4896    3686 
   Size:       1210    4584
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5794
 
BfrBI ATG'CAT

Cuts at:   2557    2557 
   Size:       5794
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2837    4405     136 
   Size:        107     122      71     631     605    1165    1568    1525

  Fragments arranged by size: 

                1568    1525    1165     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5789    5789 
   Size:       5794
 
BlpI GC'TnA_GC

Cuts at:   3211    3211 
   Size:       5794
 
Bme1580I G_kGCm'C

Cuts at:   1790    2640    3855    5011    1790 
   Size:        850    1215    1156    2573

  Fragments arranged by size: 

                2573    1215    1156     850
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5794
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3525    4483     442 
   Size:        416      53      83     399      24    2108     958    1753

  Fragments arranged by size: 

                2108    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5794
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2075    2123    2124    2810    4454    1239 
   Size:        836      48       1     686    1644    2579

  Fragments arranged by size: 

                2579    1644     836     686      48       1
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2215    2702    3919    4784    5082    2215 
   Size:        487    1217     865     298    2927

  Fragments arranged by size: 

                2927    1217     865     487     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4457     882 
   Size:        617    2958    2219

  Fragments arranged by size: 

                2958    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3585     493 
   Size:        711    2381    2702

  Fragments arranged by size: 

                2702    2381     711
 
BsaBI GATnn'nnATC

Cuts at:   5788    5788 
   Size:       5794
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2020    3601    3983    4618 
   Size:         53      83     186     936     487    1581     382     635

Cuts at:   4618     275 
   Size:       1451

  Fragments arranged by size: 

                1581    1451     936     635     487     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4227    4625    4899    5046    5457    4227 
   Size:        398     274     147     411    4564

  Fragments arranged by size: 

                4564     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5569

  Fragments arranged by size: 

                5569     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2927    1186 
   Size:       1741    4053
 
BseYI C'CCAG_C

Cuts at:   2126    2967    4997    5493    2126 
   Size:        841    2030     496    2427

  Fragments arranged by size: 

                2427    2030     841     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2735    1163 
   Size:        573     999    4222

  Fragments arranged by size: 

                4222     999     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3244    4005    5033    1540 
   Size:        275     186    1243     761    1028    2301

  Fragments arranged by size: 

                2301    1243    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2466    2667    3855    3940    5011    2466 
   Size:        201    1188      85    1071    3249

  Fragments arranged by size: 

                3249    1188    1071     201      85
 
BsmI GAATG_Cn'

Cuts at:   3305    3398    3305 
   Size:         93    5701
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2133    2466    2640    2667    2889    3019    3113 
   Size:        399     343     333     174      27     222     130      94

Cuts at:   3113    3855    3940    5011    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     343     333     222     174
                 130      94      85      27
 
BspEI T'CCGG_A

Cuts at:   5457    5457 
   Size:       5794
 
BspHI T'CATG_A

Cuts at:   2667    3683    5413    2667 
   Size:       1016    1730    3048

  Fragments arranged by size: 

                3048    1730    1016
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2020    3149     844 
   Size:       1176    1129    3489

  Fragments arranged by size: 

                3489    1176    1129
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3681    1394 
   Size:        429    1858    3507

  Fragments arranged by size: 

                3507    1858     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4289    4463      65 
   Size:       1183    3041     174    1396

  Fragments arranged by size: 

                3041    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4438    4625    1700 
   Size:        110    2628     187    2869

  Fragments arranged by size: 

                2869    2628     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5794
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5794
 
BssSI C'ACGA_G

Cuts at:   3854    4866    3854 
   Size:       1012    4782
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5794
 
BstBI TT'CG_AA

Cuts at:   4598    4598 
   Size:       5794
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5794
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2305    2305 
   Size:       5794
 
BstYI r'GATC_y

Cuts at:   1972    2340    2764    3124    3491    3876    3893    5345    5431 
   Size:        368     424     360     367     385      17    1452      86

Cuts at:   5431    5443    5789    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     424     385     368     367     360     346
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2517     513 
   Size:        552     939     513    3790

  Fragments arranged by size: 

                3790     939     552     513
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5055

  Fragments arranged by size: 

                5055     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2154    3310    4104    4124    2154 
   Size:       1156     794      20    3824

  Fragments arranged by size: 

                3824    1156     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5794
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5759
 
DraI TTT'AAA

Cuts at:   3098    3098 
   Size:       5794
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3066    4801     809 
   Size:        507    1750    1735    1802

  Fragments arranged by size: 

                1802    1750    1735     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3241    4130    5759 
   Size:         54    1425      50     275      18    1411     889    1629

Cuts at:   5759       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1411     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3241    1537 
   Size:        275    1429    4090

  Fragments arranged by size: 

                4090    1429     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2102    3724    1353 
   Size:        749    1622    3423

  Fragments arranged by size: 

                3423    1622     749
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4381    4755    4901     387 
   Size:       1064     138    2792     374     146    1280

  Fragments arranged by size: 

                2792    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2665    2665 
   Size:       5794
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2075    2123    2124    2810    2934    3036    3857 
   Size:        393     443      48       1     686     124     102     821

Cuts at:   3857    4454    5241    5509    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     821     787     686     597     443     393     268
                 124     102      48       1
 
EcoRI G'AATT_C

Cuts at:   2189    3103    3481    2189 
   Size:        914     378    4502

  Fragments arranged by size: 

                4502     914     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3106    3106 
   Size:       5794
 
FspI TGC'GCA

Cuts at:   4300    4300 
   Size:       5794
 
HaeII r_GCGC'y

Cuts at:   1678    2053    4941    1678 
   Size:        375    2888    2531

  Fragments arranged by size: 

                2888    2531     375
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2196    2228    4435    4467    4509 
   Size:         32     135      32     624      32    2207      32      42

Cuts at:   4509    4541    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2207     624     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2177    3138    3384     669 
   Size:        988     281     239     961     246    3079

  Fragments arranged by size: 

                3079     988     961     281     246     239
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5794
 
HpaI GTT'AAC

Cuts at:   3384    3384 
   Size:       5794
 
KpnI G_GTAC'C

Cuts at:   3119    3119 
   Size:       5794
 
MfeI C'AATT_G

Cuts at:   2904    3393    2904 
   Size:        489    5305
 
MluI A'CGCG_T

Cuts at:   5670    5670 
   Size:       5794
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4908    5092    1305 
   Size:       3603     184    2007

  Fragments arranged by size: 

                3603    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3972

  Fragments arranged by size: 

                3972    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3752    4111    4270     518 
   Size:        648    2586     359     159    2042

  Fragments arranged by size: 

                2586    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2967    3210    3887    5035    5280    1606 
   Size:       1361     243     677    1148     245    2120

  Fragments arranged by size: 

                2120    1361    1148     677     245     243
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4303

  Fragments arranged by size: 

                4303     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5794
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5794
 
NotI GC'GGCC_GC

Cuts at:   3241    3241 
   Size:       5794
 
NsiI A_TGCA'T

Cuts at:   2559    2559 
   Size:       5794
 
NspI r_CATG'y

Cuts at:   1132    1229    2557    2989    3152    4697    1132 
   Size:         97    1328     432     163    1545    2229

  Fragments arranged by size: 

                2229    1545    1328     432     163      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5794
 
PciI A'CATG_T

Cuts at:   4693    4693 
   Size:       5794
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2811    3000    1108 
   Size:       1703     189    3902

  Fragments arranged by size: 

                3902    1703     189
 
PmeI GTTT'AAAC

Cuts at:   3098    3098 
   Size:       5794
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3863    3895    5376    5408    3863 
   Size:         32    1481      32    4249

  Fragments arranged by size: 

                4249    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2791    1613 
   Size:       1178    4616
 
PsiI TTA'TAA

Cuts at:   3364    3364 
   Size:       5794
 
PspOMI G'GGCC_C

Cuts at:   2636    2636 
   Size:       5794
 
PstI C_TGCA'G

Cuts at:    830    3146     830 
   Size:       2316    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5794
 
PvuII CAG'CTG

Cuts at:   2967    2967 
   Size:       5794
 
SacI G_AGCT'C

Cuts at:   2667    2667 
   Size:       5794
 
SalI G'TCGA_C

Cuts at:   1936    3136    1936 
   Size:       1200    4594
 
SbfI CC_TGCA'GG

Cuts at:   3146    3146 
   Size:       5794
 
ScaI AGT'ACT

Cuts at:   4042    5754    4042 
   Size:       1712    4082
 
SfcI C'TryA_G

Cuts at:    826    1045    3142    4277    4958    5149     826 
   Size:        219    2097    1135     681     191    1471

  Fragments arranged by size: 

                2097    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5794
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5794
 
SmaI CCC'GGG

Cuts at:   1872    2025    3121    1872 
   Size:        153    1096    4545

  Fragments arranged by size: 

                4545    1096     153
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2194    2681    3898    4799    5061     820 
   Size:        197     926     251     487    1217     901     262    1553

  Fragments arranged by size: 

                1553    1217     926     901     487     262     251     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3585     493 
   Size:       3092    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5794
 
SphI G_CATG'C

Cuts at:   1132    3152    1132 
   Size:       2020    3774
 
SspI AAT'ATT

Cuts at:      5      52    3718       5 
   Size:         47    3666    2081

  Fragments arranged by size: 

                3666    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2840    1517 
   Size:       1323    4471
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3233     513 
   Size:        552     939    1229    3074

  Fragments arranged by size: 

                3074    1229     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2175    3821    3838    3991    4176    4621     325 
   Size:       1255     595    1646      17     153     185     445    1498

  Fragments arranged by size: 

                1646    1498    1255     595     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4040    5752      96 
   Size:        274      80      33      51    1443    2063    1712     138

  Fragments arranged by size: 

                2063    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2246    2278    5754    5786    2246 
   Size:         32    3476      32    2254

  Fragments arranged by size: 

                3476    2254      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5794
 
XbaI T'CTAG_A

Cuts at:   3130    3130 
   Size:       5794
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5794
 
XmaI C'CCGG_G

Cuts at:   1870    2023    3119    1870 
   Size:        153    1096    4545

  Fragments arranged by size: 

                4545    1096     153
 
XmnI GAAnn'nnTTC

Cuts at:   3923    3923 
   Size:       5794
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3602     276 
   Size:         53      83     186     936    2068    2468

  Fragments arranged by size: 

                2468    2068     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SacI     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI     BplI
   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII    EcoNI EcoO109I
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI    RsrII    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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