(Circular) (Six-base) MAPSORT of: pFN21AE1448.seq Check: 7216 from: 1 to: 5794 pFN21AE1448 5794 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2020 2020 Size: 5794 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3137 1937 Size: 1200 4594 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 3036 3857 5241 5509 1632 Size: 1302 102 821 1384 268 1917 Fragments arranged by size: 1917 1384 1302 821 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5597 AflIII A'CryG_T Cuts at: 1203 4693 5670 1203 Size: 3490 977 1327 Fragments arranged by size: 3490 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5794 AlwNI CAG_nnn'CTG Cuts at: 2722 3537 5109 2722 Size: 815 1572 3407 Fragments arranged by size: 3407 1572 815 ApaI G_GGCC'C Cuts at: 2640 2640 Size: 5794 ApaLI G'TGCA_C Cuts at: 3851 5007 3851 Size: 1156 4638 ApoI r'AATT_y Cuts at: 1493 2189 3103 3329 3481 5590 1493 Size: 696 914 226 152 2109 1697 Fragments arranged by size: 2109 1697 914 696 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 2023 3119 1870 Size: 73 80 1096 4545 Fragments arranged by size: 4545 1096 80 73 BamHI G'GATC_C Cuts at: 3124 3491 3124 Size: 367 5427 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 3115 4570 618 Size: 325 217 627 221 1107 1455 1842 Fragments arranged by size: 1842 1455 1107 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2133 2640 2667 2889 3019 3113 1391 Size: 742 507 27 222 130 94 4072 Fragments arranged by size: 4072 742 507 222 130 94 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2660 928 Size: 971 761 4062 Fragments arranged by size: 4062 971 761 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3985 4019 1903 Size: 34 2048 34 3678 Fragments arranged by size: 3678 2048 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 1548 Size: 5794 BfrBI ATG'CAT Cuts at: 2557 2557 Size: 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2837 4405 136 Size: 107 122 71 631 605 1165 1568 1525 Fragments arranged by size: 1568 1525 1165 631 605 122 107 71 BglII A'GATC_T Cuts at: 5789 5789 Size: 5794 BlpI GC'TnA_GC Cuts at: 3211 3211 Size: 5794 Bme1580I G_kGCm'C Cuts at: 1790 2640 3855 5011 1790 Size: 850 1215 1156 2573 Fragments arranged by size: 2573 1215 1156 850 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3525 4483 442 Size: 416 53 83 399 24 2108 958 1753 Fragments arranged by size: 2108 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2123 2124 2810 4454 1239 Size: 836 48 1 686 1644 2579 Fragments arranged by size: 2579 1644 836 686 48 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2215 2702 3919 4784 5082 2215 Size: 487 1217 865 298 2927 Fragments arranged by size: 2927 1217 865 487 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4457 882 Size: 617 2958 2219 Fragments arranged by size: 2958 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3585 493 Size: 711 2381 2702 Fragments arranged by size: 2702 2381 711 BsaBI GATnn'nnATC Cuts at: 5788 5788 Size: 5794 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2020 3601 3983 4618 Size: 53 83 186 936 487 1581 382 635 Cuts at: 4618 275 Size: 1451 Fragments arranged by size: 1581 1451 936 635 487 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4227 4625 4899 5046 5457 4227 Size: 398 274 147 411 4564 Fragments arranged by size: 4564 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5569 Fragments arranged by size: 5569 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2927 1186 Size: 1741 4053 BseYI C'CCAG_C Cuts at: 2126 2967 4997 5493 2126 Size: 841 2030 496 2427 Fragments arranged by size: 2427 2030 841 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2735 1163 Size: 573 999 4222 Fragments arranged by size: 4222 999 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3244 4005 5033 1540 Size: 275 186 1243 761 1028 2301 Fragments arranged by size: 2301 1243 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2466 2667 3855 3940 5011 2466 Size: 201 1188 85 1071 3249 Fragments arranged by size: 3249 1188 1071 201 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2466 2640 2667 2889 3019 3113 Size: 399 343 333 174 27 222 130 94 Cuts at: 3113 3855 3940 5011 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 333 222 174 130 94 85 27 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 2667 3683 5413 2667 Size: 1016 1730 3048 Fragments arranged by size: 3048 1730 1016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2020 3149 844 Size: 1176 1129 3489 Fragments arranged by size: 3489 1176 1129 BsrBI CCG'CTC Cuts at: 1394 1823 3681 1394 Size: 429 1858 3507 Fragments arranged by size: 3507 1858 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4289 4463 65 Size: 1183 3041 174 1396 Fragments arranged by size: 3041 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4438 4625 1700 Size: 110 2628 187 2869 Fragments arranged by size: 2869 2628 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5794 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 3854 4866 3854 Size: 1012 4782 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5794 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5794 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 5794 BstYI r'GATC_y Cuts at: 1972 2340 2764 3124 3491 3876 3893 5345 5431 Size: 368 424 360 367 385 17 1452 86 Cuts at: 5431 5443 5789 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 424 385 368 367 360 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2517 513 Size: 552 939 513 3790 Fragments arranged by size: 3790 939 552 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 2154 3310 4104 4124 2154 Size: 1156 794 20 3824 Fragments arranged by size: 3824 1156 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5759 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3066 4801 809 Size: 507 1750 1735 1802 Fragments arranged by size: 1802 1750 1735 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3241 4130 5759 Size: 54 1425 50 275 18 1411 889 1629 Cuts at: 5759 8 Size: 43 Fragments arranged by size: 1629 1425 1411 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3241 1537 Size: 275 1429 4090 Fragments arranged by size: 4090 1429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 3724 1353 Size: 749 1622 3423 Fragments arranged by size: 3423 1622 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4381 4755 4901 387 Size: 1064 138 2792 374 146 1280 Fragments arranged by size: 2792 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2665 2665 Size: 5794 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2123 2124 2810 2934 3036 3857 Size: 393 443 48 1 686 124 102 821 Cuts at: 3857 4454 5241 5509 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 821 787 686 597 443 393 268 124 102 48 1 EcoRI G'AATT_C Cuts at: 2189 3103 3481 2189 Size: 914 378 4502 Fragments arranged by size: 4502 914 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3106 3106 Size: 5794 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 2053 4941 1678 Size: 375 2888 2531 Fragments arranged by size: 2888 2531 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2196 2228 4435 4467 4509 Size: 32 135 32 624 32 2207 32 42 Cuts at: 4509 4541 1373 Size: 32 2626 Fragments arranged by size: 2626 2207 624 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2177 3138 3384 669 Size: 988 281 239 961 246 3079 Fragments arranged by size: 3079 988 961 281 246 239 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 2904 3393 2904 Size: 489 5305 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4908 5092 1305 Size: 3603 184 2007 Fragments arranged by size: 3603 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3972 Fragments arranged by size: 3972 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3752 4111 4270 518 Size: 648 2586 359 159 2042 Fragments arranged by size: 2586 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2967 3210 3887 5035 5280 1606 Size: 1361 243 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 677 245 243 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4303 Fragments arranged by size: 4303 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NsiI A_TGCA'T Cuts at: 2559 2559 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 2557 2989 3152 4697 1132 Size: 97 1328 432 163 1545 2229 Fragments arranged by size: 2229 1545 1328 432 163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5794 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 2811 3000 1108 Size: 1703 189 3902 Fragments arranged by size: 3902 1703 189 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3863 3895 5376 5408 3863 Size: 32 1481 32 4249 Fragments arranged by size: 4249 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2791 1613 Size: 1178 4616 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspOMI G'GGCC_C Cuts at: 2636 2636 Size: 5794 PstI C_TGCA'G Cuts at: 830 3146 830 Size: 2316 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 PvuII CAG'CTG Cuts at: 2967 2967 Size: 5794 SacI G_AGCT'C Cuts at: 2667 2667 Size: 5794 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SfcI C'TryA_G Cuts at: 826 1045 3142 4277 4958 5149 826 Size: 219 2097 1135 681 191 1471 Fragments arranged by size: 2097 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5794 SmaI CCC'GGG Cuts at: 1872 2025 3121 1872 Size: 153 1096 4545 Fragments arranged by size: 4545 1096 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 2194 2681 3898 4799 5061 820 Size: 197 926 251 487 1217 901 262 1553 Fragments arranged by size: 1553 1217 926 901 487 262 251 197 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 3152 1132 Size: 2020 3774 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 2840 1517 Size: 1323 4471 StyI C'CwwG_G Cuts at: 513 1065 2004 3233 513 Size: 552 939 1229 3074 Fragments arranged by size: 3074 1229 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2175 3821 3838 3991 4176 4621 325 Size: 1255 595 1646 17 153 185 445 1498 Fragments arranged by size: 1646 1498 1255 595 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4040 5752 96 Size: 274 80 33 51 1443 2063 1712 138 Fragments arranged by size: 2063 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2246 2278 5754 5786 2246 Size: 32 3476 32 2254 Fragments arranged by size: 3476 2254 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5794 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5794 XmaI C'CCGG_G Cuts at: 1870 2023 3119 1870 Size: 153 1096 4545 Fragments arranged by size: 4545 1096 153 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI