################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 91 CGCG CG^CG AcyI 304 GACGCC G[GA]^CG[CT]C AlwI 66 GATCC GGATC(4/5) Asp700I 111 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 148 GGCACC G^G[CT][GA]CC BmyI 231 GGGCAC G[AGT]GC[ACT]^C BsaHI 304 GACGCC G[GA]^CG[CT]C BsaI 167 GAGACC GGTCTC(1/5) BseRI 127 CTCCTC GAGGAG(10/8) BsmAI 167 GAGAC GTCTC(1/5) Bsp1286I 231 GGGCAC G[AGT]GC[ACT]^C BssSI 217 CTCGTG CACGAG(-5/-1) BstNI 81 CCAGG CC^[AT]GG BstUI 91 CGCG CG^CG Cac8I 254 GCCCGC GC[ACGT]^[ACGT]GC Cfr13I 213 GGGCC G^G[ACGT]CC DpnII 66 GATC ^GATC DsaI 239 CCGTGG C^C[GA][CT]GG EcoRII 81 CCAGG ^CC[AT]GG FauI 255 CCCGC CCCGC(4/6) HgaI 304 GACGC GACGC(5/10) Hin1I 304 GACGCC G[GA]^CG[CT]C HinfI 104 GAATC G^A[ACGT]TC MaeII 235 ACGT A^CGT MboI 66 GATC ^GATC MslI 234 CACGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 208 CAGCTG C[AC]G^C[GT]G MvaI 81 CCAGG CC^[AT]GG MvnI 91 CGCG CG^CG NdeII 66 GATC ^GATC NlaIV 148 GGCACC GG[ACGT]^[ACGT]CC NspI 161 ACATGC [GA]CATG^[CT] PvuII 208 CAGCTG CAG^CTG Sau3AI 66 GATC ^GATC Sau96I 213 GGGCC G^G[ACGT]CC SfaNI 32 GCATC GCATC(5/9) TfiI 104 GAATC G^A[AT]TC TspRI 99 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 111 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 188 AGCT AG^CT 209 AGCT BbvI 207 GCAGC GCAGC(8/12) 297 GCAGC BsrI 98 CCAGT ACTGG(1/-1) 180 CCAGT BstF5I 276 CATCC GGATG(2/0) 291 CATCC FokI 276 CATCC GGATG(9/13) 291 CATCC MboII 15 GAAGA GAAGA(8/7) 18 GAAGA NlaIII 162 CATG CATG^ 267 CATG TaqI 56 TCGA T^CGA 119 TCGA TseI 207 GCAGC G^C[AT]GC 297 GCAGC TthHB8I 56 TCGA T^CGA 119 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AciI AfaI BcnI BsaJI BsiYI BslI BssKI Fnu4HI HaeIII HapII HpaII ItaI MnlI MspI NciI RsaI ScrFI