################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BamHI BanII BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI Bst1107I BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HindIII HinfI KpnI MaeI MamI MfeI MflI MluI MluNI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspI PacI PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SfiI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 1397 TCCGGA T^CCGGA AcyI 1228 GGCGCC G[GA]^CG[CT]C AflIII 833 ACGTGT A^C[GA][CT]GT Alw44I 668 GTGCAC G^TGCAC ApaLI 668 GTGCAC G^TGCAC Asp700I 171 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1116 TGCGCA TGC^GCA BbeI 1228 GGCGCC GGCGC^C BcgI 627 CGAGGAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 580 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1449 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1449 GCTGAGC GC^T[ACGT]AGC BsaHI 1228 GGCGCC G[GA]^CG[CT]C BsaI 1336 GAGACC GGTCTC(1/5) BseAI 1397 TCCGGA T^CCGGA BsmAI 1336 GAGAC GTCTC(1/5) BsmFI 206 GGGAC GGGAC(10/14) BspEI 1397 TCCGGA T^CCGGA BssHII 1278 GCGCGC G^CGCGC BssSI 701 CTCGTG CACGAG(-5/-1) BstBI 787 TTCGAA TT^CGAA BstEII 16 GGTGACC G^GT[ACGT]ACC BstPI 16 GGTGACC G^GT[ACGT]ACC CelII 1449 GCTGAGC GC^T[ACGT]AGC DrdI 1075 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 790 GAAGAG CTCTTC(1/4) EcoO65I 16 GGTGACC G^GT[ACGT]ACC FspI 1116 TGCGCA TGC^GCA Hin1I 1228 GGCGCC G[GA]^CG[CT]C HincII 268 GTTAAC GT[CT]^[GA]AC HindII 268 GTTAAC GT[CT]^[GA]AC HpaI 268 GTTAAC GTT^AAC KasI 1228 GGCGCC G^GCGCC Ksp632I 790 GAAGAG CTCTTC(1/4) MroI 1397 TCCGGA T^CCGGA MseI 269 TTAA T^TAA NaeI 517 GCCGGC GCC^GGC NarI 1228 GGCGCC GG^CGCC NgoMIV 517 GCCGGC G^CCGGC NspV 787 TTCGAA TT^CGAA PstI 1001 CTGCAG CTGCA^G SapI 790 GAAGAGC GCTCTTC(1/4) ScaI 1481 AGTACT AGT^ACT SexAI 1076 ACCTGGT A^CC[AT]GGT SfoI 1228 GGCGCC GGC^GCC SfuI 787 TTCGAA TT^CGAA SgrAI 1049 CGCCGGTG C[GA]^CCGG[CT]G Tru9I 269 TTAA T^TAA XcmI 938 CCAAGGGCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 171 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 393 GATCC GGATC(4/5) 774 GATCC AvaI 6 CTCGAG C^[CT]CG[GA]G 1477 CTCGAG AvaII 447 GGACC G^G[AT]CC 1074 GGACC BbsI 460 GTCTTC GAAGAC(2/6) 1186 GTCTTC BpuAI 460 GTCTTC GAAGAC(2/6) 1186 GTCTTC BsaWI 20 ACCGGA [AT]^CCGG[AT] 1397 TCCGGA BseRI 628 GAGGAG GAGGAG(10/8) 1333 GAGGAG BsoBI 6 CTCGAG C^[CT]CG[GA]G 1477 CTCGAG BsrBI 793 GAGCGG CCGCTC(-3/-3) 1362 GAGCGG DraII 361 GGGGCCT [GA]G^G[ACGT]CC[CT] 1064 AGGCCCT EcoO109I 361 GGGGCCT [GA]G^G[ACGT]CC[CT] 1064 AGGCCCT HaeII 642 GGCGCT [GA]GCGC^[CT] 1228 GGCGCC PaeR7I 6 CTCGAG C^TCGAG 1477 CTCGAG PflMI 249 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 351 CCACAGTCTGG SfcI 366 CTACAG C^T[GA][CT]AG 1001 CTGCAG SmlI 6 CTCGAG C^T[CT][GA]AG 1477 CTCGAG Van91I 249 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 351 CCACAGTCTGG XhoI 6 CTCGAG C^TCGAG 1477 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI BanI BbvI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI KspI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I