(Circular) (Six-base) MAPSORT of: pFN21AE1505.seq Check: 4739 from: 1 to: 6193 pFN21AE1505 6193 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2602 2737 2602 Size: 135 6058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 3047 4256 5640 5908 1632 Size: 896 519 1209 1384 268 1917 Fragments arranged by size: 1917 1384 1209 896 519 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2838 5092 6069 1203 Size: 1635 2254 977 1327 Fragments arranged by size: 2254 1635 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 2546 1166 Size: 1380 4813 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2564 2937 3242 1184 Size: 1380 373 305 4135 Fragments arranged by size: 4135 1380 373 305 AlwNI CAG_nnn'CTG Cuts at: 2158 3025 3073 3295 3936 5508 2158 Size: 867 48 222 641 1572 2843 Fragments arranged by size: 2843 1572 867 641 222 48 ApaLI G'TGCA_C Cuts at: 2673 4250 5406 2673 Size: 1577 1156 3460 Fragments arranged by size: 3460 1577 1156 ApoI r'AATT_y Cuts at: 1493 3502 3728 3880 5989 1493 Size: 2009 226 152 2109 1697 Fragments arranged by size: 2109 2009 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2011 3482 3518 1870 Size: 73 68 1471 36 4545 Fragments arranged by size: 4545 1471 73 68 36 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3058 3233 3358 3389 3514 Size: 325 217 627 1271 175 125 31 125 Cuts at: 3514 4969 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1271 627 325 217 175 125 125 31 BanII G_rGCy'C Cuts at: 1391 3512 1391 Size: 2121 4072 BbeI G_GCGC'C Cuts at: 3237 3237 Size: 6193 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2458 3184 928 Size: 971 559 726 3937 Fragments arranged by size: 3937 971 726 559 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2621 2655 4384 4418 1903 Size: 34 684 34 1729 34 3678 Fragments arranged by size: 3678 1729 684 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2591 4804 136 Size: 107 122 71 631 605 919 2213 1525 Fragments arranged by size: 2213 1525 919 631 605 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 3455 3610 3455 Size: 155 6038 Bme1580I G_kGCm'C Cuts at: 1790 2677 3053 4254 5410 1790 Size: 887 376 1201 1156 2573 Fragments arranged by size: 2573 1201 1156 887 376 BmgBI CAC'GTC Cuts at: 1318 2410 2656 1318 Size: 1092 246 4855 Fragments arranged by size: 4855 1092 246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2560 2687 3218 Size: 416 53 83 399 24 1143 127 531 Cuts at: 3218 3924 4882 442 Size: 706 958 1753 Fragments arranged by size: 1753 1143 958 706 531 416 399 127 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 3356 3467 4853 1239 Size: 929 1188 111 1386 2579 Fragments arranged by size: 2579 1386 1188 929 111 Bpu10I CC'TnA_GC Cuts at: 3288 3288 Size: 6193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4318 5183 5481 4318 Size: 865 298 5030 Fragments arranged by size: 5030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3335 4856 882 Size: 617 1836 1521 2219 Fragments arranged by size: 2219 1836 1521 617 BsaAI yAC'GTr Cuts at: 493 1204 3984 493 Size: 711 2780 2702 Fragments arranged by size: 2780 2702 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3234 4000 4382 5017 Size: 53 83 186 936 1701 766 382 635 Cuts at: 5017 275 Size: 1451 Fragments arranged by size: 1701 1451 936 766 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2025 3402 4626 5024 5298 5445 5856 2025 Size: 1377 1224 398 274 147 411 2362 Fragments arranged by size: 2362 1377 1224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2924 2954 1178 Size: 30 165 30 1521 30 4417 Fragments arranged by size: 4417 1521 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 3353 1186 Size: 1462 705 4026 Fragments arranged by size: 4026 1462 705 BseYI C'CCAG_C Cuts at: 2599 3295 5396 5892 2599 Size: 696 2101 496 2900 Fragments arranged by size: 2900 2101 696 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2630 2771 3032 3248 3278 1163 Size: 573 414 480 141 261 216 30 4078 Fragments arranged by size: 4078 573 480 414 261 216 141 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2018 2553 2677 2883 4254 4339 5410 2018 Size: 535 124 206 1371 85 1071 2801 Fragments arranged by size: 2801 1371 1071 535 206 124 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2553 2677 2883 3053 3512 4254 Size: 399 228 535 124 206 170 459 742 Cuts at: 4254 4339 5410 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 535 459 399 228 206 170 124 85 BspEI T'CCGG_A Cuts at: 3402 5856 3402 Size: 2454 3739 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2602 2737 3178 3450 3548 844 Size: 1758 135 441 272 98 3489 Fragments arranged by size: 3489 1758 441 272 135 98 BsrBI CCG'CTC Cuts at: 1394 1823 2800 3369 4080 1394 Size: 429 977 569 711 3507 Fragments arranged by size: 3507 977 711 569 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2522 3055 3344 4837 5024 1700 Size: 110 712 533 289 1493 187 2869 Fragments arranged by size: 2869 1493 712 533 289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 3283 1925 Size: 1358 4835 BssSI C'ACGA_G Cuts at: 2706 4253 5265 2706 Size: 1547 1012 3634 Fragments arranged by size: 3634 1547 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2158 3022 3148 3216 794 Size: 1364 864 126 68 3771 Fragments arranged by size: 3771 1364 864 126 68 BstBI TT'CG_AA Cuts at: 2793 4997 2793 Size: 2204 3989 BstEII G'GTnAC_C Cuts at: 1184 2021 1184 Size: 837 5356 BstYI r'GATC_y Cuts at: 1972 3523 3890 4275 4292 5744 5830 5842 6188 Size: 1551 367 385 17 1452 86 12 346 Cuts at: 6188 1972 Size: 1977 Fragments arranged by size: 1977 1551 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2847 2971 3269 513 Size: 552 939 843 124 298 3437 Fragments arranged by size: 3437 939 843 552 298 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2502 487 Size: 683 56 1276 4178 Fragments arranged by size: 4178 1276 683 56 BtsI GCAGTG_nn' Cuts at: 2118 2613 2695 3015 3153 3709 4503 4523 2118 Size: 495 82 320 138 556 794 20 3788 Fragments arranged by size: 3788 794 556 495 320 138 82 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3086 5200 809 Size: 507 1770 2114 1802 Fragments arranged by size: 2114 1802 1770 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2520 2583 2658 Size: 54 1425 50 275 18 690 63 75 Cuts at: 2658 3165 3640 4529 6158 8 Size: 507 475 889 1629 43 Fragments arranged by size: 1629 1425 889 690 507 475 275 75 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2790 4123 1353 Size: 1437 1333 3423 Fragments arranged by size: 3423 1437 1333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2342 2831 4780 5154 5300 387 Size: 1064 138 753 489 1949 374 146 1280 Fragments arranged by size: 1949 1280 1064 753 489 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2528 3047 3356 3467 4256 4853 Size: 393 536 360 519 309 111 789 597 Cuts at: 4853 5640 5908 1239 Size: 787 268 1524 Fragments arranged by size: 1524 789 787 597 536 519 393 360 309 268 111 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2630 3335 2252 Size: 378 705 5110 Fragments arranged by size: 5110 705 378 EcoO109I rG'GnC_Cy Cuts at: 2367 3070 2367 Size: 703 5490 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FspI TGC'GCA Cuts at: 3123 4699 3123 Size: 1576 4617 HaeII r_GCGC'y Cuts at: 1678 2651 3237 5340 1678 Size: 973 586 2103 2531 Fragments arranged by size: 2531 2103 973 586 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4834 4866 4908 4940 1373 Size: 32 135 32 3262 32 42 32 2626 Fragments arranged by size: 3262 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2275 3537 3783 669 Size: 988 281 337 1262 246 3079 Fragments arranged by size: 3079 1262 988 337 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 2275 3783 2275 Size: 1508 4685 KasI G'GCGC_C Cuts at: 3233 3233 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5307 5491 1305 Size: 4002 184 2007 Fragments arranged by size: 4002 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2546 2888 3353 4151 4510 4669 518 Size: 648 1380 342 465 798 359 159 2042 Fragments arranged by size: 2042 1380 798 648 465 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2395 2849 2973 3216 3262 3271 3382 3609 Size: 789 454 124 243 46 9 111 227 Cuts at: 3609 4286 5434 5679 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 789 677 454 245 243 227 124 111 46 9 NaeI GCC'GGC Cuts at: 2524 2524 Size: 6193 NarI GG'CG_CC Cuts at: 3234 3234 Size: 6193 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4702 Fragments arranged by size: 4702 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NgoMIV G'CCGG_C Cuts at: 2522 2522 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3551 5096 1132 Size: 97 2322 1545 2229 Fragments arranged by size: 2322 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 2260 2362 1108 Size: 1152 102 4939 Fragments arranged by size: 4939 1152 102 PfoI T'CCnGG_A Cuts at: 2400 3333 2400 Size: 933 5260 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2951 2983 4262 4294 5775 5807 2951 Size: 32 1279 32 1481 32 3337 Fragments arranged by size: 3337 1481 1279 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspXI vC'TCGA_Gb Cuts at: 2011 3482 2011 Size: 1471 4722 PstI C_TGCA'G Cuts at: 830 3010 3545 830 Size: 2180 535 3478 Fragments arranged by size: 3478 2180 535 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 2395 3216 3262 2395 Size: 821 46 5326 Fragments arranged by size: 5326 821 46 SacII CC_GC'GG Cuts at: 2850 2974 3272 2850 Size: 124 298 5771 Fragments arranged by size: 5771 298 124 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SapI GCTCTTCn'nnn_ Cuts at: 2790 2790 Size: 6193 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 3488 4441 6153 3488 Size: 953 1712 3528 Fragments arranged by size: 3528 1712 953 SexAI A'CCwGG_T Cuts at: 3081 3081 Size: 6193 SfcI C'TryA_G Cuts at: 826 1045 2371 3006 3541 4676 5357 5548 826 Size: 219 1326 635 535 1135 681 191 1471 Fragments arranged by size: 1471 1326 1135 681 635 535 219 191 SfoI GGC'GCC Cuts at: 3235 3235 Size: 6193 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 3055 1700 Size: 1355 4838 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2011 3482 4297 5198 5460 820 Size: 197 926 68 1471 815 901 262 1553 Fragments arranged by size: 1553 1471 926 901 815 262 197 68 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2004 2139 2943 3168 3423 3632 513 Size: 552 939 135 804 225 255 209 3074 Fragments arranged by size: 3074 939 804 552 255 225 209 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4237 4390 4575 5020 325 Size: 1255 2640 17 153 185 445 1498 Fragments arranged by size: 2640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3240 3486 4439 Size: 274 80 33 51 1443 1263 246 953 Cuts at: 4439 6151 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1263 953 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2924 2956 6153 6185 2924 Size: 32 3197 32 2932 Fragments arranged by size: 3197 2932 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2950 2950 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 2011 3482 1943 Size: 68 1471 4654 Fragments arranged by size: 4654 1471 68 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 2180 4322 2180 Size: 2142 4051 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SanDI SfiI SrfI SwaI