(Circular) (Six-base) MAPSORT of: pFN21AE5408.seq Check: 132 from: 1 to: 5713 pFN21AE5408 5713 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2524 2524 Size: 5713 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3056 1937 Size: 1119 4594 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 3776 5160 5428 1632 Size: 569 1575 1384 268 1917 Fragments arranged by size: 1917 1575 1384 569 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5516 AflIII A'CryG_T Cuts at: 1203 2753 4612 5589 1203 Size: 1550 1859 977 1327 Fragments arranged by size: 1859 1550 1327 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 2899 4442 2899 Size: 1543 4170 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2492 1184 Size: 1308 4405 AlwNI CAG_nnn'CTG Cuts at: 2017 2170 2227 2239 3456 5028 2017 Size: 153 57 12 1217 1572 2702 Fragments arranged by size: 2702 1572 1217 153 57 12 ApaLI G'TGCA_C Cuts at: 2477 2876 3770 4926 2477 Size: 399 894 1156 3264 Fragments arranged by size: 3264 1156 894 399 ApoI r'AATT_y Cuts at: 1493 2723 2828 3022 3248 3400 5509 1493 Size: 1230 105 194 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 226 194 152 105 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 2293 3038 1870 Size: 73 350 745 4545 Fragments arranged by size: 4545 745 350 73 BamHI G'GATC_C Cuts at: 3043 3410 3043 Size: 367 5346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 3034 4489 618 Size: 325 217 627 265 982 1455 1842 Fragments arranged by size: 1842 1455 982 627 325 265 217 BanII G_rGCy'C Cuts at: 1391 2736 3032 1391 Size: 1345 296 4072 Fragments arranged by size: 4072 1345 296 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2101 2116 2602 928 Size: 971 202 15 486 4039 Fragments arranged by size: 4039 971 486 202 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3904 3938 1903 Size: 34 50 34 1883 34 3678 Fragments arranged by size: 3678 1883 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4815 3605 Size: 1210 4503 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4324 136 Size: 107 122 71 631 605 2652 1525 Fragments arranged by size: 2652 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 2282 3130 2282 Size: 848 4865 Bme1580I G_kGCm'C Cuts at: 1790 2379 2481 2880 3774 4930 1790 Size: 589 102 399 894 1156 2573 Fragments arranged by size: 2573 1156 894 589 399 102 BmgBI CAC'GTC Cuts at: 1318 2482 1318 Size: 1164 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2317 2971 3444 Size: 416 53 83 399 24 900 654 473 Cuts at: 3444 4402 442 Size: 958 1753 Fragments arranged by size: 1753 958 900 654 473 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2273 2642 4373 1239 Size: 1010 24 369 1731 2579 Fragments arranged by size: 2579 1731 1010 369 24 Bpu10I CC'TnA_GC Cuts at: 2672 2672 Size: 5713 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3838 4703 5001 3838 Size: 865 298 4550 Fragments arranged by size: 4550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4376 882 Size: 617 2877 2219 Fragments arranged by size: 2877 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2754 3504 493 Size: 711 1550 750 2702 Fragments arranged by size: 2702 1550 750 711 BsaBI GATnn'nnATC Cuts at: 2747 5707 2747 Size: 2960 2753 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3520 3902 4537 275 Size: 53 83 186 936 1987 382 635 1451 Fragments arranged by size: 1987 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 4544 4818 4965 5376 4146 Size: 398 274 147 411 4483 Fragments arranged by size: 4483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 2303 2333 1178 Size: 30 165 30 789 30 81 30 4558 Fragments arranged by size: 4558 789 165 81 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2119 2213 2899 1186 Size: 933 94 686 4000 Fragments arranged by size: 4000 933 686 94 BseYI C'CCAG_C Cuts at: 2086 2517 4916 5412 2086 Size: 431 2399 496 2387 Fragments arranged by size: 2399 2387 496 431 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5140 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2604 3163 3924 4952 1540 Size: 275 186 603 559 761 1028 2301 Fragments arranged by size: 2301 1028 761 603 559 275 186 BsiHKAI G_wGCw'C Cuts at: 2205 2283 2349 2481 2562 2678 2736 2880 3774 Size: 78 66 132 81 116 58 144 894 Cuts at: 3774 3859 4930 2205 Size: 85 1071 2988 Fragments arranged by size: 2988 1071 894 144 132 116 85 81 78 66 58 BsmI GAATG_Cn' Cuts at: 2427 3224 3317 2427 Size: 797 93 4823 Fragments arranged by size: 4823 797 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2283 2349 2379 2481 2562 2678 Size: 399 415 78 66 30 102 81 116 Cuts at: 2678 2736 2880 3032 3774 3859 4930 1391 Size: 58 144 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 152 144 116 102 85 81 78 66 58 30 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 3602 5332 3602 Size: 1730 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2524 2769 3068 844 Size: 1319 361 245 299 3489 Fragments arranged by size: 3489 1319 361 299 245 BsrBI CCG'CTC Cuts at: 1394 1823 2050 2058 3600 1394 Size: 429 227 8 1542 3507 Fragments arranged by size: 3507 1542 429 227 8 BsrDI GCAATG_nn' Cuts at: 65 1248 4208 4382 65 Size: 1183 2960 174 1396 Fragments arranged by size: 2960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2494 4357 4544 1700 Size: 110 211 473 1863 187 2869 Fragments arranged by size: 2869 1863 473 211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 2029 2204 3773 4785 2029 Size: 175 1569 1012 2957 Fragments arranged by size: 2957 1569 1012 175 BstAPI GCAn_nnn'nTGC Cuts at: 794 2031 794 Size: 1237 4476 BstBI TT'CG_AA Cuts at: 4517 4517 Size: 5713 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstXI CCAn_nnnn'nTGG Cuts at: 2536 2686 2930 2536 Size: 150 244 5319 Fragments arranged by size: 5319 244 150 BstYI r'GATC_y Cuts at: 1972 3043 3410 3795 3812 5264 5350 5362 5708 Size: 1071 367 385 17 1452 86 12 346 Cuts at: 5708 1972 Size: 1977 Fragments arranged by size: 1977 1452 1071 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4974 Fragments arranged by size: 4974 683 56 BtsI GCAGTG_nn' Cuts at: 2124 3229 4023 4043 2124 Size: 1105 794 20 3794 Fragments arranged by size: 3794 1105 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5678 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DraIII CAC_nnn'GTG Cuts at: 2374 2374 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2365 2541 Size: 54 1425 50 275 18 189 346 176 Cuts at: 2541 2601 2970 3160 4049 5678 8 Size: 60 369 190 889 1629 43 Fragments arranged by size: 1629 1425 889 369 346 275 190 189 176 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2601 3160 1537 Size: 275 789 559 4090 Fragments arranged by size: 4090 789 559 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 3643 1353 Size: 980 1310 3423 Fragments arranged by size: 3423 1310 980 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4300 4674 4820 387 Size: 1064 138 2711 374 146 1280 Fragments arranged by size: 2711 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2734 2734 Size: 5713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2249 2273 2642 3776 4373 5160 Size: 393 569 48 24 369 1134 597 787 Cuts at: 5160 5428 1239 Size: 268 1524 Fragments arranged by size: 1524 1134 787 597 569 393 369 268 48 24 EcoNI CCTnn'n_nnAGG Cuts at: 2706 2706 Size: 5713 EcoO109I rG'GnC_Cy Cuts at: 2152 2152 Size: 5713 EcoRI G'AATT_C Cuts at: 3022 3400 3022 Size: 378 5335 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 5713 FspI TGC'GCA Cuts at: 2174 4219 2174 Size: 2045 3668 HaeII r_GCGC'y Cuts at: 1678 4860 1678 Size: 3182 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2303 2335 2885 Size: 32 135 32 626 32 73 32 550 Cuts at: 2885 2917 4354 4386 4428 4460 1373 Size: 32 1437 32 42 32 2626 Fragments arranged by size: 2626 1437 626 550 135 73 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4827 5011 1305 Size: 3522 184 2007 Fragments arranged by size: 3522 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2367 2543 2972 10 Size: 54 1425 343 535 176 429 2751 Fragments arranged by size: 2751 1425 535 429 343 176 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 3671 4030 4189 518 Size: 648 1308 1197 359 159 2042 Fragments arranged by size: 2042 1308 1197 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2227 2517 2606 2930 3129 3806 4954 5199 Size: 621 290 89 324 199 677 1148 245 Cuts at: 5199 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 621 324 290 245 199 89 NaeI GCC'GGC Cuts at: 2023 2023 Size: 5713 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5161 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NgoMIV G'CCGG_C Cuts at: 2021 2021 Size: 5713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 3071 4616 1132 Size: 97 1842 1545 2229 Fragments arranged by size: 2229 1842 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5713 PciI A'CATG_T Cuts at: 4612 4612 Size: 5713 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5713 PfoI T'CCnGG_A Cuts at: 2619 2640 2619 Size: 21 5692 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PmlI CAC'GTG Cuts at: 2754 2754 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2152 2152 Size: 5713 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PstI C_TGCA'G Cuts at: 830 2013 3065 830 Size: 1183 1052 3478 Fragments arranged by size: 3478 1183 1052 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 PvuII CAG'CTG Cuts at: 2227 2930 2227 Size: 703 5010 SacI G_AGCT'C Cuts at: 2736 2736 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SapI GCTCTTCn'nnn_ Cuts at: 2333 2333 Size: 5713 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SexAI A'CCwGG_T Cuts at: 2103 2103 Size: 5713 SfcI C'TryA_G Cuts at: 826 1045 2009 3061 4196 4877 5068 826 Size: 219 964 1052 1135 681 191 1471 Fragments arranged by size: 1471 1135 1052 964 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3817 4718 4980 820 Size: 197 926 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 3637 5 Size: 47 3585 2081 Fragments arranged by size: 3585 2081 47 StuI AGG'CCT Cuts at: 1517 2399 1517 Size: 882 4831 StyI C'CwwG_G Cuts at: 513 1065 2563 3152 513 Size: 552 1498 589 3074 Fragments arranged by size: 3074 1498 589 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2990 3740 3757 3910 4095 4540 325 Size: 1255 1410 750 17 153 185 445 1498 Fragments arranged by size: 1498 1410 1255 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3959 5671 96 Size: 274 80 33 51 1443 1982 1712 138 Fragments arranged by size: 1982 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2192 2224 5673 5705 2192 Size: 32 3449 32 2200 Fragments arranged by size: 3449 2200 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2536 2536 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 2828 3842 2828 Size: 1014 4699 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI