Restriction map for pFN21AE9655
 (Circular) (Six-base) MAPSORT of: pFN21AE9655.seq  Check: 3310  from: 1  to: 5089



pFN21AE9655  5089 bp

 With 182 enzymes: SgfI * 

                        July 6, 2012 17:42  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2899     278 
   Size:         53      83     186     936    1363    2468

  Fragments arranged by size: 

                2468    1363     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2432    1937 
   Size:        495    4594
 
Acc65I G'GTAC_C

Cuts at:   2410    2410 
   Size:       5089
 
AclI AA'CG_TT

Cuts at:   1563    3216    3589    1563 
   Size:       1653     373    3063

  Fragments arranged by size: 

                3063    1653     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3152    4536    4804    1632 
   Size:       1520    1384     268    1917

  Fragments arranged by size: 

                1917    1520    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4892
 
AflIII A'CryG_T

Cuts at:   1203    2140    3988    4965    1203 
   Size:        937    1848     977    1327

  Fragments arranged by size: 

                1848    1327     977     937
 
AgeI A'CCGG_T

Cuts at:   3920    3920 
   Size:       5089
 
AhdI GACnn_n'nnGTC

Cuts at:   2130    3818    2130 
   Size:       1688    3401
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5089
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5089
 
AlwNI CAG_nnn'CTG

Cuts at:   2832    4404    2832 
   Size:       1572    3517
 
ApaLI G'TGCA_C

Cuts at:   3146    4302    3146 
   Size:       1156    3933
 
ApoI r'AATT_y

Cuts at:   1493    2398    2624    2776    4885    1493 
   Size:        905     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     905     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3643     160 
   Size:        872    2611    1606

  Fragments arranged by size: 

                2611    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5089
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2414    1870 
   Size:         73     471    4545

  Fragments arranged by size: 

                4545     471      73
 
BamHI G'GATC_C

Cuts at:   2419    2786    2419 
   Size:        367    4722
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2410    3865     618 
   Size:        325     217     627     623    1455    1842

  Fragments arranged by size: 

                1842    1455     627     623     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2408    1391 
   Size:       1017    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2387     928 
   Size:        971     488    3630

  Fragments arranged by size: 

                3630     971     488
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3280    3314    1903 
   Size:         34    1343      34    3678

  Fragments arranged by size: 

                3678    1343      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2981    4191    2981 
   Size:       1210    3879
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5089
 
BfrBI ATG'CAT

Cuts at:   2222    2222 
   Size:       5089
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3700     136 
   Size:        107     122      71     631     605    2028    1525

  Fragments arranged by size: 

                2028    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5084    5084 
   Size:       5089
 
BlpI GC'TnA_GC

Cuts at:   2506    2506 
   Size:       5089
 
Bme1580I G_kGCm'C

Cuts at:   1790    3150    4306    1790 
   Size:       1360    1156    2573

  Fragments arranged by size: 

                2573    1360    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2141    1318 
   Size:        823    4266
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2084    2820    3778 
   Size:        416      53      83     399      24     667     736     958

Cuts at:   3778     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     736     667     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5089
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2201    3749    1239 
   Size:        962    1548    2579

  Fragments arranged by size: 

                2579    1548     962
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3214    4079    4377    3214 
   Size:        865     298    3926

  Fragments arranged by size: 

                3926     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3752     882 
   Size:        617    2253    2219

  Fragments arranged by size: 

                2253    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2880     493 
   Size:        711    1676    2702

  Fragments arranged by size: 

                2702    1676     711
 
BsaBI GATnn'nnATC

Cuts at:   5083    5083 
   Size:       5089
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2896    3278    3913     275 
   Size:         53      83     186     936    1363     382     635    1451

  Fragments arranged by size: 

                1451    1363     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2033    3522    3920    4194    4341    4752    2033 
   Size:       1489     398     274     147     411    2370

  Fragments arranged by size: 

                2370    1489     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4864

  Fragments arranged by size: 

                4864     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5089
 
BseYI C'CCAG_C

Cuts at:   4292    4788    4292 
   Size:        496    4593
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4516
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2539    3300    4328    1540 
   Size:        275     186     538     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     538     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3150    3235    4306    3150 
   Size:         85    1071    3933

  Fragments arranged by size: 

                3933    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2600    2693    2600 
   Size:         93    4996
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2408    3150    3235    4306    1391 
   Size:        399     618     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     618     399      85
 
BspEI T'CCGG_A

Cuts at:   2033    4752    2033 
   Size:       2719    2370
 
BspHI T'CATG_A

Cuts at:   2978    4708    2978 
   Size:       1730    3359
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2444     844 
   Size:       1600    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2064    2976    1394 
   Size:        429     241     912    3507

  Fragments arranged by size: 

                3507     912     429     241
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3584    3758      65 
   Size:       1183    2336     174    1396

  Fragments arranged by size: 

                2336    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2075    3733    3920    1700 
   Size:        110     265    1658     187    2869

  Fragments arranged by size: 

                2869    1658     265     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2163      96 
   Size:       2067    3022
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5089
 
BssSI C'ACGA_G

Cuts at:   3149    4161    3149 
   Size:       1012    4077
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2219     794 
   Size:       1425    3664
 
BstBI TT'CG_AA

Cuts at:   3893    3893 
   Size:       5089
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5089
 
BstYI r'GATC_y

Cuts at:   1972    2419    2786    3171    3188    4640    4726    4738    5084 
   Size:        447     367     385      17    1452      86      12     346

Cuts at:   5084    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     447     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3598

  Fragments arranged by size: 

                3598     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4350

  Fragments arranged by size: 

                4350     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2605    3399    3419    2605 
   Size:        794      20    4275

  Fragments arranged by size: 

                4275     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5089
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5054
 
DraI TTT'AAA

Cuts at:   2393    2393 
   Size:       5089
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4096     809 
   Size:        507    2780    1802

  Fragments arranged by size: 

                2780    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2536    3425    5054 
   Size:         54    1425      50     275      18     706     889    1629

Cuts at:   5054       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     706     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2536    1537 
   Size:        275     724    4090

  Fragments arranged by size: 

                4090     724     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3019    1353 
   Size:       1666    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3676    4050    4196     387 
   Size:       1064     138    2087     374     146    1280

  Fragments arranged by size: 

                2087    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2201    3152    3749    4536    4804    1239 
   Size:        393     569     951     597     787     268    1524

  Fragments arranged by size: 

                1524     951     787     597     569     393     268
 
EcoRI G'AATT_C

Cuts at:   2398    2776    2398 
   Size:        378    4711
 
FspI TGC'GCA

Cuts at:   3595    3595 
   Size:       5089
 
HaeII r_GCGC'y

Cuts at:   1678    4236    1678 
   Size:       2558    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2116    2148    3730    3762    3804 
   Size:         32     135      32     544      32    1582      32      42

Cuts at:   3804    3836    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1582     544     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2433    2679     669 
   Size:        988     281     495     246    3079

  Fragments arranged by size: 

                3079     988     495     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5089
 
HpaI GTT'AAC

Cuts at:   2679    2679 
   Size:       5089
 
KpnI G_GTAC'C

Cuts at:   2414    2414 
   Size:       5089
 
MfeI C'AATT_G

Cuts at:   2316    2688    2316 
   Size:        372    4717
 
MluI A'CGCG_T

Cuts at:   4965    4965 
   Size:       5089
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4203    4387    1305 
   Size:       2898     184    2007

  Fragments arranged by size: 

                2898    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3267

  Fragments arranged by size: 

                3267    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2139    3047    3406    3565     518 
   Size:        648     973     908     359     159    2042

  Fragments arranged by size: 

                2042     973     908     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2159    2505    3182    4330    4575    1606 
   Size:        553     346     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     553     346     245
 
NaeI GCC'GGC

Cuts at:   2077    2077 
   Size:       5089
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3598

  Fragments arranged by size: 

                3598     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5089
 
NgoMIV G'CCGG_C

Cuts at:   2075    2075 
   Size:       5089
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5089
 
NotI GC'GGCC_GC

Cuts at:   2536    2536 
   Size:       5089
 
NsiI A_TGCA'T

Cuts at:   2224    2224 
   Size:       5089
 
NspI r_CATG'y

Cuts at:   1132    1229    2447    3992    1132 
   Size:         97    1218    1545    2229

  Fragments arranged by size: 

                2229    1545    1218      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5089
 
PciI A'CATG_T

Cuts at:   3988    3988 
   Size:       5089
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5089
 
PfoI T'CCnGG_A

Cuts at:   2178    2178 
   Size:       5089
 
PmeI GTTT'AAAC

Cuts at:   2393    2393 
   Size:       5089
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3158    3190    4671    4703    3158 
   Size:         32    1481      32    3544

  Fragments arranged by size: 

                3544    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5089
 
PsiI TTA'TAA

Cuts at:   2659    2659 
   Size:       5089
 
PstI C_TGCA'G

Cuts at:    830    2441     830 
   Size:       1611    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5089
 
SalI G'TCGA_C

Cuts at:   1936    2431    1936 
   Size:        495    4594
 
SbfI CC_TGCA'GG

Cuts at:   2441    2441 
   Size:       5089
 
ScaI AGT'ACT

Cuts at:   3337    5049    3337 
   Size:       1712    3377
 
SfcI C'TryA_G

Cuts at:    826    1045    2437    3572    4253    4444     826 
   Size:        219    1392    1135     681     191    1471

  Fragments arranged by size: 

                1471    1392    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5089
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5089
 
SmaI CCC'GGG

Cuts at:   1872    2416    1872 
   Size:        544    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3193    4094    4356     820 
   Size:        197     926    1250     901     262    1553

  Fragments arranged by size: 

                1553    1250     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2880     493 
   Size:       2387    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5089
 
SphI G_CATG'C

Cuts at:   1132    2447    1132 
   Size:       1315    3774
 
SspI AAT'ATT

Cuts at:      5      52    3013       5 
   Size:         47    2961    2081

  Fragments arranged by size: 

                2961    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5089
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2528     513 
   Size:        552     939     524    3074

  Fragments arranged by size: 

                3074     939     552     524
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3116    3133    3286    3471    3916     325 
   Size:       1255    1536      17     153     185     445    1498

  Fragments arranged by size: 

                1536    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2163    3335    5047 
   Size:        274      80      33      51    1443     186    1172    1712

Cuts at:   5047      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1172     274     186     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5049    5081    5049 
   Size:         32    5057
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5089
 
XbaI T'CTAG_A

Cuts at:   2425    2425 
   Size:       5089
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5089
 
XmaI C'CCGG_G

Cuts at:   1870    2414    1870 
   Size:        544    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3218    3218 
   Size:       5089
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2897     276 
   Size:         53      83     186     936    1363    2468

  Fragments arranged by size: 

                2468    1363     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NarI     NruI     PacI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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